A. Ataei Azimi; B. Delnavaz Hashemloian
Abstract
In cytology studies, colors are used to differentiate the components of cells that are transparent. Most of the dyes are synthetic and can harm human and environment. Herbal dyes are safe. Since ancient times in Iran, the natural dye of madder (Rubia tinctorum L.) has been used in the textile industry. ...
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In cytology studies, colors are used to differentiate the components of cells that are transparent. Most of the dyes are synthetic and can harm human and environment. Herbal dyes are safe. Since ancient times in Iran, the natural dye of madder (Rubia tinctorum L.) has been used in the textile industry. In this research, the madder root dye was extracted with water, ethanol and acid at the end of spring, summer and autumn and its colors were separated by paper chromatography. Acetoronacin was prepared from the dye extracted from madder. Acetoronacin was used to stain epidermal cells, stomata guard cells, pollen, and the plant mitosis. The results showed that the best solvent to isolate the dye of madder was water. Chromatography results showed that the type and number of colors (5 colors) in the aqueous extract of madder root in the three seasons were similar, but some of them were higher at the end of the summer. Soluble colors in alcohol (3 colors) were observed in the ethanolic extract at the end of spring. Only one yellow color, probably alizarin, was isolated from the acid extract. Staining the cells with acetoronacin in all cases showed that the color was comparable and even better than acetocarmine. Nuclei and chromosomes of cells were stained brown by staining with acetoronacin in a transparent background. In general, the results showed that this dye can replace the synthetic dyes in the plant cell research laboratories.
N. Hosseinpour Azad; Gh.A. Nematzadeh; M. Azadbakht; S.K. Kazemitabar; E. Shokri
Abstract
In this study, two important physical and chemical properties including percentage of total seed oil and fatty acid levels in two ecotypes of Iranian ox tongue flower (Echium amoenum Fisch & Mey.) collected from Kermanshah and Neka ...
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In this study, two important physical and chemical properties including percentage of total seed oil and fatty acid levels in two ecotypes of Iranian ox tongue flower (Echium amoenum Fisch & Mey.) collected from Kermanshah and Neka (Mazandaran province) were investigated. Oil extraction and identification of fatty acids were conducted by soxhelt system and Gas Chromatography (GC). All data were compared by statistical methods. Results showed that total oil percentage of (19.16 ± 0.31%) & (17.16 ± 0.26) were calculated for Kermanshah and Neka ecotypes, respectively. Also 8 types of fatty acids including palmitic, stearic, oleic, soxenic, linoleic, alpha-linolenic, gamma-linolenic and stearidonic acids were detected by GC. Soxenic acid and alpha-linolenic were respectively identified as the least (0.2±0.4%) and highest amount (46±48%) in each ecotype. The obtained data indicate that with regard to the nutritional values of essential fatty acids in seed oil, Iranian ox tongue flower has a good potential for producing nutritional supplements of essential fatty acids i-e., omega3 and omega6. According to the results of the study, obtained data from the current study could be applied as a reference in selection of suitable breeding methods to improve oil quality and quantity of Iranian ox tongue flower.
S.M. Seyyedi; H. Rakhshandeh; H. Sadeghian
Abstract
In this research, the extraction and separation of sedative compounds of Rosa damascena Mill. (from Rosaceae family) was studied. The minimum dosage of sedative effect was the basis of physiological tests (on Balb-C mouse) which has done in MashhadUniversity of medical sciences. Several extractions were ...
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In this research, the extraction and separation of sedative compounds of Rosa damascena Mill. (from Rosaceae family) was studied. The minimum dosage of sedative effect was the basis of physiological tests (on Balb-C mouse) which has done in MashhadUniversity of medical sciences. Several extractions were done by ethanol, chloroform and water, among which the extracted syrup from ethanol showed the best effect (500mg/kg). This syrup showed five main spots on TLC (thin layer chromatography), from which the desired compound was separated by column chromatography. The physiological tests of the pure compound from column chromatography showed that the minimum effective dosage was 15mg/kg. The chemical structure of separated compounds was also studied by Mass, IR and NMR spectroscopy.
M. Naderi Hagybaghercandy; M.B. Rezaee; K. Jaimand
Volume 19, Issue 2 , August 2003, , Pages 149-156
Abstract
Gum arabic or gum acacia is oldest and best known of all the natural gums. Gum arabic is one of carbohydrat`s gum group that is nutritive and demulcent, and exerts a soothing influence upon irritated or inflamed mucous tissues, by shielding them from the influence of deleterrious agents, atmospheric ...
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Gum arabic or gum acacia is oldest and best known of all the natural gums. Gum arabic is one of carbohydrat`s gum group that is nutritive and demulcent, and exerts a soothing influence upon irritated or inflamed mucous tissues, by shielding them from the influence of deleterrious agents, atmospheric air, etc In this investigation gum of acacia nilotica plant collected from farm of medicinal plant Research in Booshehr province for study quantitative and qualitative. In this study quantitative, determination of carbohydrate has done by Antron method by spectrophotometry (HITACHI 340 ) in 625 nm. The percent of total carbohydrates in gum arabic is 6.51 %. Also in this study qualitative specified that there are pentoses and hexoses and uronic acid in gum arabic by TLC method. In this method used– methylethylketone several reagentes and solventes that best is formic acid – tertiary butanol – water and anisidine – phthalic acid – ethanol.
