Biotechnology
S.M. Mostafavi; M.R. Abdollahi; D. Dastan; H. Sarikhani
Abstract
Caper (Capparis spinosa L.) is a rich source of rutin, plays an essential role in human health. In the present study, the effects of cold (25°C as control, 4°C, and 7°C for 2, 4, and 7 days), heat (25°C as control, 30°C for 14 days, 32°C for 2 and 4 days, and 35°C for 8 hours), ...
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Caper (Capparis spinosa L.) is a rich source of rutin, plays an essential role in human health. In the present study, the effects of cold (25°C as control, 4°C, and 7°C for 2, 4, and 7 days), heat (25°C as control, 30°C for 14 days, 32°C for 2 and 4 days, and 35°C for 8 hours), and carbohydrate treatments on the androgenesis efficiency were studied in the anther culture of caper. Also, the effects of maltose and sucrose at the concentrations of 30 and 60 g L-1 in combination with two temperature treatments (1- 30°C for 14 days and 2- 7°C for 7 days + azacytidine and 2,4-D pretreatments) on the androgenesis induction was evaluated. The temperature and carbohydrate treatments showed statistically significant differences (p < /em>≤0.01) in terms of callus and embryo formation. The 7°C for 2, 4, and 7 days produced the highest percentage (at the third week: 80, 78.34, and 76.67%, respectively) and callogenesis speed (7.85, 7.75, and 7.60 calli week-1, respectively) and the 7°C for 7 days produced the highest embryo production (0.57 embryo anther-1). The 30°C for 14 days treatment showed the highest percentage (at the third week: 100%) and callogenesis speed (9.44 calli week-1). While the 32°C for 2 and 3 days and also 30°C for 14 days produced the highest number of embryos per anther (0.22, 0.20, and 0.18 embryo, respectively). The use of 30 g L-1 maltose in combination with the 30°C for 14 days produced the highest percentage (at the third week: 91.66%) and callogenesis speed (8.94 calli week-1), while the 30 g L-1 maltose in combination with the 7°C for 7 days + azacytidine and 2,4-D pretreatments produced the highest mean embryo number per anther (0.55 embryo). The results of this research are of great importance for the use in the caper breeding programs.
S. Saffarpour; M.H. Givianrad; P. Beheshti
Abstract
This paper is the first report on detection and determination of antioxidant compounds in seed oil of medicinal and edible shrub of Capparis spinosa L. collected from Dashte-Moghan. The results are expressed as the mean of three separate replications. Seed oil was extracted by hexane in a soxhelt apparatus ...
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This paper is the first report on detection and determination of antioxidant compounds in seed oil of medicinal and edible shrub of Capparis spinosa L. collected from Dashte-Moghan. The results are expressed as the mean of three separate replications. Seed oil was extracted by hexane in a soxhelt apparatus and 2.36% was recorded as the total percentage of unsaponifiable matter. Detection and determination of some of the components of the unsaponifiable matter which have antioxidant properties shows that 0.27 % of the oil is related to sterols. β-sitosterol (60%), campestrol (13%) and stigmasterol (10%) are as the most abundant sterols and this oil has a great potential source of -avenasterol (7%). Moreover, the sterol composition of this oil is comparable to peanut oil and olive oil. Total content of tocopherols and tocotrienols was 93.43 ppm which is too close to olive oil. In addition, g-isomer is predominant tocopherol. This oil is rich of beta-carotene (280mg/kg) that is close to the unbleached palm stearin. The total phenolic compounds were ignorable.