Agriculture and horticulture
F. Darabi; N. Abbasi; M.J. Zarea
Abstract
To investigate the role of putrescine and 24-epibrassinolide on the morphophysiological changes of Ocimum basilicum L. in the water deficiency tolerance, an experiment was conducted as a factorial split plot in a randomized complete block design with three replications in the research farm of Agriculture ...
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To investigate the role of putrescine and 24-epibrassinolide on the morphophysiological changes of Ocimum basilicum L. in the water deficiency tolerance, an experiment was conducted as a factorial split plot in a randomized complete block design with three replications in the research farm of Agriculture Faculty, Ilam University during 2017-19 crop years. The experimental treatments included the drought stress at three levels (40, 80, and 120 mm evaporation from Class A evaporation pan) in the main plots and putrescine foliar application at three levels (Put0: 0, Put1: 0.5, and Put2: 2 mM) and 24-epibrassinolide foliar application at three levels (Br0: 0, Br1: 0.5, and Br2: 2 μM) factorially in the subplots. The results showed that the multiple interaction of year, drought stress, putrescine, and 24-epibrassinolide had a significant effect on the traits including chlorophyll a, chlorophyll b, and basil essential oil percentage, so that the foliar application of 2 mM putrescine and 2 μM 24-epibrassinolide at all levels of drought stress, especially 80 mm evaporation from the evaporation pan, caused a further increase in these traits in both years of the experiment, which was higher in the first year than the second one. The optimal irrigation treatment (40 mm evaporation from evaporation pan) with application of 2 mM putrescine and 0.5 μM 24-epibrassinolide and drought stress treatments (80 and 120 mm evaporation from evaporation pan) with application of 2 mM putrescine and 2 μM 24-epibrassinolide had more leaves and secondary branches. The results of this study showed that the drought stress had an important role in reducing the basic factors in basil growth. In general, according to the results obtained, the foliar application of 2 mM putrescine with 2 μM 24-epibrassinolide to improve the physiological activities and increase the essential oil content, and 2 mM putrescine with 0.5 μM 24-epibrassinolide to increase the seed yield could be recommended in basil under drought stress conditions.
D. Rasouli; B. Fakheri
Abstract
In order to evaluate the effects of drought stress on quantity and quality of yield, physiological characteristics, and essential oil of Ocimum basilicum L. and Ocimum americanum L., a factorial experiment was carried out in a randomized complete block design with three replications in greenhouse condition. ...
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In order to evaluate the effects of drought stress on quantity and quality of yield, physiological characteristics, and essential oil of Ocimum basilicum L. and Ocimum americanum L., a factorial experiment was carried out in a randomized complete block design with three replications in greenhouse condition. Drought stress treatments consisted of control (without drought stress), 80, 60, and 40% of field capacity on two species of Ocimum. The traits measured were fresh and dry weights, essential oil, essential oil performance, carbohydrates, proline, nitrogen, protein, phosphorus and potassium contents. The results indicated that with increasing levels of drought stress from control to 40 percent of field capacity, the content of essential oil, carbohydrates, and proline were increased (28, 46, and 50%) in both species. However, the content of fresh and dry weight, essential performance, nitrogen, protein, phosphorus and potassium were reduced (57, 57.8, 44, 19, 16, 39 and 23%). In O. americanum, the content of fresh and dry weight, carbohydrate, proline, nitrogen, phosphorus, and protein were significantly increased under drought stress; while in O. basilicum, only essential oil content showed higher values significantly. It was observed that the majority of growth factors had the highest performance under low stress (80% of field capacity). Essential oil content, on the other hand, increased significantly under high stress condition, so that in O. basilicum, the highest essential oil content was obtained at 60 percent of field capacity. Our findings indicated that the plant performance and essential oil content were increased under average and moderately severe stress conditions, respectively.
M. Peyvandi; H. Parandeh; M. Mirza
Abstract
Sweet basil (Ocimum basilicum L.) belongs to the Lamiaceae family. Sweet basil essential oil is used in food, medicine, and hygiene industries. In this study, the effect of different concentrations of nano iron chelate fertilizer (1, 3, 5 Kg.ha-1) and iron chelate fertilizer (1.5, 4.5, 7.5kg.ha-1) on ...
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Sweet basil (Ocimum basilicum L.) belongs to the Lamiaceae family. Sweet basil essential oil is used in food, medicine, and hygiene industries. In this study, the effect of different concentrations of nano iron chelate fertilizer (1, 3, 5 Kg.ha-1) and iron chelate fertilizer (1.5, 4.5, 7.5kg.ha-1) on the quantity and quality of O. basilicum essential oil (EO) were investigated. The experiment was conducted in a completely randomized design with four replications in a farm located in Saveh. After collecting the aerial parts of O. balilicum, the EO was extracted via water distillation method. Then, the EO was analyzed by GC and GC/MS. Sixteen components were identified in the EO of O. basilicum. The major components were methyl chavicol (37%), geranial (26%), neral (21%) and caryophyllene oxide (3.5%), respectively. Among different treatments, the means of 10 components showed significant differences. The mean of EO percentage in all samples, treated by Fe and nano Fe fertilizers, was more than that of control.
H. Tehrani Sharif; E. Sharifi Ashoorabadi; A.A. Tajali; M. Makizadeh Tafti
Abstract
In order to evaluate soil fertilization systems on yield quantity and quality of basil (Ocimum basilicum L.), this study was conducted as a field experiment in Shahrerey Islamic Azad University in 2011. The experiment was conducted in split plots in time based on completely randomized block design with ...
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In order to evaluate soil fertilization systems on yield quantity and quality of basil (Ocimum basilicum L.), this study was conducted as a field experiment in Shahrerey Islamic Azad University in 2011. The experiment was conducted in split plots in time based on completely randomized block design with three replications. The treatments were various levels of chemical fertilizers (N-P-K), different levels of manure, mixture of different ratios of fertilize and manure and without fertilizer and manure (control). Two harvests were arranged in main plots and 16 levels of fertilizer were assigned to the sub plots. According to the results, the highest amount of dry yield (2442 kgha-1) was obtained in second harvest and in intermediate treatment (25 tonha-1 of manure mixed with N=80, P=64 and K=80 kgha-1 of chemical fertilizers). Results showed that the intermediate treatment (10 tonha-1 of manure mixed with N=120, P=96 and K=120 kgha-1 of chemical fertilizers) in second harvest produced the highest amount of essential oil (0.69%). The results suggest that the use of organic fertilizer alone or in combination with chemical fertilizers has a positive effect in improving the yield quantity and quality of basil.
M.R. Shahraki; H. Mirshekari; A.R Shahraki; E. Shahraki
Abstract
Since Ocimum basilicumL. is used as a traditional herbal medicine, this study was carried out to evaluate the effect of aqueous and metanolic extract of Ocimum basilicum leaf on blood glucose, lipid profiles and liver enzymes in fructose-fed male rats. The experiment was performed on 32 Wistar-Albino ...
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Since Ocimum basilicumL. is used as a traditional herbal medicine, this study was carried out to evaluate the effect of aqueous and metanolic extract of Ocimum basilicum leaf on blood glucose, lipid profiles and liver enzymes in fructose-fed male rats. The experiment was performed on 32 Wistar-Albino rats including one control group (A) and three tests groups (B, C and D). Test groups consumed fructose-enriched water and received zero and 20mg/kg aqueous and metabolic extract daily and respectively for 60 days but control group consumed tap water in treatment duration. At the end, serum glucose, insulin, liver enzymes and lipid profile were measured. The obtained data were analyzed by ANOVA and Tukey tests. Results were expressed as mean ± SE and significant differences were found at 0.05% level of probability. Our findings showed that insulin resistance index, insulin and FBS in tests groups significantly increased compared with those of group A; however, serum insulin value in group D significantly increased compared with that of group B and C. On the other hand, AST (aspartate aminotransfrase) and ALT (alanine amino transferase) values in group D significantly decreased compared with those of the B and C groups. Also, the value of LDL in group C was significantly lower in comparison to other test groups. The results of this study showed that consumption of Ocimum basilicumleaf extract had an effect on LDL and liver enzymes in fructose-fed male rats.
V. Weisany; S. Rahimzadeh; Y. Sohrabi
Abstract
Fertilizer management is utmost important factor in success of pharmaceutical crops cultivation. Application of biological fertilizers is important in production of these plants with the aim of elimination or significant reduction of chemical inputs and also increase of soil fertility and improvement ...
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Fertilizer management is utmost important factor in success of pharmaceutical crops cultivation. Application of biological fertilizers is important in production of these plants with the aim of elimination or significant reduction of chemical inputs and also increase of soil fertility and improvement of plant growth and quality. Therefore, an experiment was conducted on basil (Ocimum basilicum L.) in greenhouse of Agriculture Faculty of Agriculture of Kurdistan University. A complete randomized design with five treatments and four replications were used. Treatments were nitroxin, biological phosphorus, nitroxin+biological phosphorus, chemical origin of nitrogen+phosphorus and control (no fertilizer). The results indicated significant effect of treatments on the dry weight of total shoots, plant height, number of leaves, leaf dry weight, photosynthesis, transpiration, essential oil content and yield, root dry weight, number of flowering branches and chlorophyll content. But, this effect was not significant on root length, stem dry weigh and sub-stomata CO2. The plant performanced better with application of chemical fertilizer and a mixture of nitroxin and biological phosphorus in terms of the mentioned criteria. Therefore, it could be concluded that biological fertilizers may be considered as a suitable replacement for a lot of chemical fertilizers consumption in sustainable agricultural systems.
Z. Aslani; A. Hassani; M. Rasooli Sadaghiyani; F. Sefidkon; M. Barin
Abstract
To study the influence of arbuscular mycorrhizal (AM) fungi inoculation and drought stress on growth, chlorophyll content and phosphorus uptake of basil (Ocimum basilicum L.), a pot experiment was conducted using a factorial based on randomized complete blocks design with four replications. The factors ...
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To study the influence of arbuscular mycorrhizal (AM) fungi inoculation and drought stress on growth, chlorophyll content and phosphorus uptake of basil (Ocimum basilicum L.), a pot experiment was conducted using a factorial based on randomized complete blocks design with four replications. The factors were mycorrhizal inoculation (non-inoculated and inoculated with Glomus mosseae T.H. Nicolson & Gerd. and Glomus intraradices N.C. Schenck & G.S. Sm) and three irrigation regimes (irrigation intervals were every 4, 8 and 12 days). The results showed that drought stress had significant effects on evaluated characteristics. As with decrease of the soil water content, plant height, stem diameter, number and area of leaves, number of axillary shoot, root dry weight, fresh and dry herb yield, chlorophyll content, P concentration in leaves and root colonization decreased. Also, AM fungi inoculation had significant effects on growth parameters and P uptake. Plants inoculated with AM fungi had higher growth, yield and P uptake than non-inoculated plants under droughtstress and non-stress conditions. G. mosseae was more effective than G. intraradices in alleviation of drought stress. It could be concluded that AM fungi are able to enhance the growth and yield of basil under drought stress condition through enhancing P uptake.
