F. Najafi; S. Mehrabian; R. Khavari-Nejad; Y. Ghorbani
Abstract
In the present study, the effects of different concentrations of NaCl (0, 15, 30, 45, 60, 75 and 100mM) were investigated on some physiological parameters and antibacterial activities of dill (Anethum graveolens L.). Ten-days-old seedlings, prepared in sterilized petri dishes, were transferred into pots ...
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In the present study, the effects of different concentrations of NaCl (0, 15, 30, 45, 60, 75 and 100mM) were investigated on some physiological parameters and antibacterial activities of dill (Anethum graveolens L.). Ten-days-old seedlings, prepared in sterilized petri dishes, were transferred into pots containing washed sand and irrigated with Hoagland nutrient solution. Pots were located in a growth chamber (at 25°C for 16h in light and 18°C for 8h in darkness) and after certain growth period, plants were harvested for analyses. For antibacterial analyses, the plants were treated throughout flowering and fruit production stages. Results showed that in all treated plants, proline content was increased and photosynthetic pigments were decreased in high concentrations of NaCl. Also, the studies on growth factors showed that in dill, these parameters were affected by NaCl. Studies on antibacterial activities showed that in most of the cases, the zone of inhibition was increased by increasing of salt concentration and the antibacterial effect of extract was significant in high concentrations. Of course, choosing the best way and solute for preparing the extract is important for reaching a noticeable antibacterial effect.
M. Lotfi; B. Abbaszadeh; M. Mirza
Abstract
In this study the effect of drought stress on morphological traits, metabolites proline accumulation, soluble carbohydrates and the yield of Tarragon (Artemisia dracunculus L.), was evaluated in order to produce Tarragon in drought stress condition as a medicinal plant, and also determine the threshold ...
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In this study the effect of drought stress on morphological traits, metabolites proline accumulation, soluble carbohydrates and the yield of Tarragon (Artemisia dracunculus L.), was evaluated in order to produce Tarragon in drought stress condition as a medicinal plant, and also determine the threshold of stress tolerance. This research was done using randomized complete blocks design with 4 treatments and 3 replications. The stress treatments were included T1 (100% of field capacity), T2 (80% of field capacity), T3 (60% of field capacity) and T4 (40% of field capacity). According to the results of statistical analysis, the drought stress had significant effect on morphological traits, flowering shoot yield, proline and soluble carbohydrates. In addition, the enhancement of drought stress led to decrease in plant height, canopy diameter, leaf length, leaf width and leaf surface, stem diameter, the length of longest lateral shoot, root length and its development, shoot yield and dry leaf yield. Comparison of treatment means showed that the highest plant height, canopy diameter, leaf length, leaf width and leaf surface, stem diameter, the length of longest lateral shoot, root length, shoot yield and dry leaf yield were related to T1 (non stress); in addition, the highest amount of proline, soluble carbohydrates and root development were related to T4 (40%FC). Therefore, drought stress had negative effect on most morphological traits and flowering shoot yield; however, it led to increase in the root length, proline and soluble carbohydrates of flowering shoots.
M. Ghorbanli; A. Alibabaee; M. Payvandi
Abstract
Hypericum perforatum L. is a valuable medicinal species. The plant geneus includes 17 species in Iran, but H. perforatum is the only valuable species.The species plays an essential role in the treatment of depression. In this study, Hypericum perforatum was treated with different concentrations of molybdenum ...
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Hypericum perforatum L. is a valuable medicinal species. The plant geneus includes 17 species in Iran, but H. perforatum is the only valuable species.The species plays an essential role in the treatment of depression. In this study, Hypericum perforatum was treated with different concentrations of molybdenum including (control, 0.1, 0.4, 0.8, 1.6 µΜ) in Hoagland solution at 6.8 pH and then samples were used to measure biochemical parameters. Molybdenum significantly increased proline content. The shoot catalase and ascorbate peroxidase activities increased in all treatments of molybdenum, but in root, it was reduced in higher concentrations. Shoot peroxidase activities increased by treatments of molybdenum, except at a concentration of 0.8µΜ. The increase in peroxidase activity was also observed in roots but it was reduced at higher concentrations. The malondealdehyde and molybdenum content increased in higher concentrations of molybdenum. Molybdenum content increased by increasing treated molybdenum. Therefore, molybdenum stress increased the proline content and peroxidase, catalase and ascorbate peroxidase activities, and toxicity symptoms were observed in high concentrations of molybdenum as lipid peroxidation. Data were analyzed by SPSS and mean comparisons was performed by Duncan's multiple range test. In each experiment, 3 replications were used.
M.R. Ardakani; B. Abbaszadeh; M.H. Assare; F. Paknezhad; A. Kashani; M. Layegh Haghighi
Abstract
One of the methods of natural resource management is utilization of inefficient lands and use of resistant plant species in hard conditions. In this study, sampling from natural habitat in Arak (Markazi province) was conducted in 2009 at 3 growth stages including growth, full flowering and maturity using ...
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One of the methods of natural resource management is utilization of inefficient lands and use of resistant plant species in hard conditions. In this study, sampling from natural habitat in Arak (Markazi province) was conducted in 2009 at 3 growth stages including growth, full flowering and maturity using plots with replication. In full flowering stage, plant height, number of tillers, big and small diameter, root length, root weight, chlorophyll, plant dry weight, soluble sugar content, proline, sodium, potassium, magnesium, calcium, chlorine and iron were measured. Mean comparison of morphological characters showed that the largest values of plant height, number of tillers, canopy cover, total shoot and flowering shoot were recorded for the region (locality 3) with less salinity (EC= 7.6 dS/m). The largest values of root length, root weight and essential oil percentage at growthing, full flowering and maturity stages were also recorded for locality 1 with high salinity (EC= 11.7dS/m). Locality 1 showed the highest content of soluble sugar, proline, sodium, potassium, magnesium, calcium, and chlorine. Locality 3 had the highest total content of chlorophyll, chlorophyll a and b while the highest iron content was related to locality 2. Results of correlation showed that there was negative significant relationship between plants height with essential oil percentage in full flowering stage. Significant correlation was also observed between soluble sugar and proline, magnesium, calcium and chlorine. There was significant negative correlation between soluble sugar and total chlorophyll 2. According to the results, Camphorosma monspilica L. is one of the salt tolerant plant species probably with medicinal and forage values.
M. Ghorbanli; F. Ahmadi; A. Monfared; Gh. Bakhshi Khaniki
Abstract
Ascorbate as a strong antioxidant has a considerable bio effect on growth of plants, such as increase in their tolerance against environment stresses. In this investigation, the effect of salt stress and its interaction with ascorbate on amount of proline, catalase and ascorbate peroxidase enzymes and ...
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Ascorbate as a strong antioxidant has a considerable bio effect on growth of plants, such as increase in their tolerance against environment stresses. In this investigation, the effect of salt stress and its interaction with ascorbate on amount of proline, catalase and ascorbate peroxidase enzymes and MDA in a medicinal plant of Cuminum cyminum L. was randomly studied in the green house conditions with three replications. Plants were treated by different concentrations of NaCl (0, 25, 50, 75, 100, 125 mmolar) and ascorbate (9 mmolar). In the plants treated with salt, the amount of proline, antioxidant enzymes activity and MDA were increased with increasing NaCl concentrations. The plants treated with NaCl and ascorbate at the same time in a same NaCl concentration, the amount of proline, catalase activity, ascorbate peroxidase and MDA were increased. The results indicated that the ascorbate was one of the antioxidants that caused an increase in resistance of Cuminum cyminum L. to salt stress.
M. Ghorbanli; Gh. Bakhshi Khaniki; A. Zakeri
Abstract
Water deficit is one of the most important factors affecting the growth of plants. In this research, Linum usitatissimum L. seeds were sown in plastic pots containing sand, clay and peat (2: 1: 1). When the third leave was appeared, water stress was done at three levels of control, 1/3 field capacity ...
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Water deficit is one of the most important factors affecting the growth of plants. In this research, Linum usitatissimum L. seeds were sown in plastic pots containing sand, clay and peat (2: 1: 1). When the third leave was appeared, water stress was done at three levels of control, 1/3 field capacity and 2/3 field capacity for 10 days in three duplication on the basis completely randomized design. The amount of carotenoid, anthocyanin, flavonoid, phenolic compound and proline was measured to study the effect of drought stress on non-enzymatic defense mechanisms. Data were subjected to analysis of variance by SPSS statistical software and means were compared by Duncan's test at p ≤ 0.05 significance level. It is noteworthy to state that proline accumulation in aerial parts of the Linum was more than that of the root. The results showed that the amount of praline, phenolic compound and anthocyanin significantly increased but the amount of flavonoid in 2/3 FC increased and then slightly decreased however it increased as compared with the control. In the current study, the amount of carotenoid in the leaves of Linum significantly decreased.
F. Salimi; F. Shekari; M.R. Azimi; E. Zangani
Abstract
To evaluate the effect of spraying with methyl jasmonate on membrance properties, ion uptake and osmotic adjustment and flower dry weight of chamomile (Matricaria chamomilla L.) under salt stress an experiment was conducted at greenhouse with three replications. The experimental design was factorial ...
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To evaluate the effect of spraying with methyl jasmonate on membrance properties, ion uptake and osmotic adjustment and flower dry weight of chamomile (Matricaria chamomilla L.) under salt stress an experiment was conducted at greenhouse with three replications. The experimental design was factorial based on randomized complete blocks design. The studied factors were as follows: spraying with methyl jasmonate at five levels including, 0, 75, 150, 225 and 300 μM in three growth stages: 1) 3 to 4 leaves, 2) stem elongation and 3) flowering (anthesis) and salt stress applied at four levels of 2.1, 6, 10 and 14 dS/m NaCl. The results showed that spraying with methyl jasmonate and salinity stress had significant effects (p ≤ 0.05) on all measured traits. The highest cell membrane stability, proline, K+, Ca+content, ratio of K+/Na+ and flower dry weight, with the lowest content of Na+ were obtained in spraying 75 µM MeJA and 6dS/m salinity stress. High amount of the mentioned parameters at the concentration of 6 dS/m salinity could be related to the assisting role of MeJA through preventing salt entrance and preferential absorption of K+ in mild salinities compared with the control treatment. In addition, application of MeJA induced significant increase in proline content. It seems that increase in proline content leads to cell membrane stability and salt resistance.
M. Ghorbanli; ْا. Bakhshi Khaniki; S Salimi Elizei; M. Hedayati
Abstract
In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: ...
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In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: 1) Control with irrigation equal to field capacity, 2) two third of field capacity, 3) one third of field capacity, 4) two third of field capacity along with 10 mM ascorbic acid, 5) two third of field capacity along with 1 mM ascorbic acid, 6) one third of field capacity along with 10 mM ascorbic acid, 7) one third of field capacity along with 1 mM ascorbic acid. Proline and soluble sugars levels in shoots and roots were significantly different for all treatments except for the treatment with 2/3 field capacity along with 10 mM ascorbic acid. Enzymes levels in 1/3 and 2/3 treatments were significantly different in both shoots and roots. One mM ascorbic acid along with different field capacity showed no reducing effect. However, 10 mM ascorbic acid especially in 2/3 field capacity could reduce the effect of water deficit through reduction in level of metabolites produced in response to low irrigation.
K. Babaee; M. Amini Dehaghi; S.A.M. Modares Sanavi; R. Jabbari
Abstract
Water deficit stress, permanent or temporary, limits the growth and the distribution of natural vegetation and the performance of cultivated plants more than any other environmental factors. Mechanism involved is still not clear. Thyme (Thymus vulgaris L.) is an important medicinal plant in perfume and ...
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Water deficit stress, permanent or temporary, limits the growth and the distribution of natural vegetation and the performance of cultivated plants more than any other environmental factors. Mechanism involved is still not clear. Thyme (Thymus vulgaris L.) is an important medicinal plant in perfume and medical industry. In this research, the effects of water deficit stress on growth, yield, metabolites compatible (prolin) and oil composition of thyme (thymol) were studied. Complete randomized block design with 4 treatments and 4 replications have been used in 2006. Water deficit levels included control (100% field capacity), mild stress (85% field capacity), medium stress (70% field capacity) and severe stress (55% field capacity). The statistical analysis showed that water deficit had significant effect on growth parameters, yield biomass, prolin and thymol. Water stress decreased plant height, number of secondary branches, dry and fresh weight growth and root mass, dry and fresh weight root and length root. In addition, thymol percentage and prolin content increased with severe stress (55% field capacity). Morphological yield with secondary metabolites prolin and thymol were obversed with water deficit changes.
B. Abbaszadeh; E. Sharifi Ashourabadi; M.H. Lebaschi; M. Naderi hajibagher Kandy; F. Moghadami
Abstract
In this research effect of drought stress on physiological characteristics of balm (Melissa officinalis L.) was performed under field condition in Karaj, Iran in 2005. Experiment was conducted using randomized complete block design with 4 replications. Treatment included T1 (non stress), T2 (80%FC), ...
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In this research effect of drought stress on physiological characteristics of balm (Melissa officinalis L.) was performed under field condition in Karaj, Iran in 2005. Experiment was conducted using randomized complete block design with 4 replications. Treatment included T1 (non stress), T2 (80%FC), T3 (60%FC), T4 (40%FC) and T5 (20%FC). Effect of drought stress on chlorophyll a, chlorophyll b, proline, soluble sugars, relative water contents, percentage and yield of essential oil were significant (α=0.001). Effect of drought stress on total chlorophyll was significant (α=0.005). Comparison of treatment means showed that highest chlorophyll a (1.921mg/l), chlorophyll b (1.197mg/l), total chlorophyll (2/283mg/l) were related to T1 (control), T5 (20%FC) and T1 (control), respectively. Highest accumulation of proline with 2.545 mg/l mean was related to T3 (60%FC). Maximum relative water content with 93.369 mean percentage related to T1 (control). It could be concluded that plant accumulation of proline and solublesugars increased tolerance and showed that moderate drought stress is beneficial for balm production.
Z. Baher Nik; M. Mirza; B. Abbaszadeh; M. Naderi Hajy Bagher Candy
Volume 23, Issue 3 , November 2007, , Pages 315-322
Abstract
Since the relationship between soil and water status of plants and its effect on growth, propagation and metabolism process, the effect of water stress on Parthenium argetatum, was investigated. Irrigation treatments were selected based on different percentage of field capacity (FC), including (1)a control ...
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Since the relationship between soil and water status of plants and its effect on growth, propagation and metabolism process, the effect of water stress on Parthenium argetatum, was investigated. Irrigation treatments were selected based on different percentage of field capacity (FC), including (1)a control sample which was irrigated to full field capacity during the growing season (FC), (2) two moderate water stress treatments (LS1=75% and LS2=50% of field capacity) and (3) severe water stress treatments (HS=25% of field capacity). Results showed that plant RWC (relative water content) decreased from 65.8% to 42.8%. Also water stress induced high amount of sugar and proline. The amount of sugar was the highest in sever treatment (HS=2.9 mg/g FW) while it was the least in FC treatment (1.07 mg/g FW). The amount of proline was the highest in sever treatment (HS= 4.28 mM) while it changed from 1.1 in LS1, 1.56 in LS2 and the least (0.96 mM) in FC treatments.
H. Aliabadi Farahani; M.H. Lebaschi; A.H. Shiranirad; S.A.R. Valadabadi; A. Hamidi; A. Alizadeh Sahzabi
Volume 23, Issue 3 , November 2007, , Pages 405-415
Abstract
In order to study the effects of Glomus hoi fungi, different levels of phosphorus and drought stress on some physiological characteristics of coriander (Coriandrum sativum L.), was conducted at Karaj Research Institute of Forest and Rangelands in 2006. Field experiment was carried out by a split factorial ...
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In order to study the effects of Glomus hoi fungi, different levels of phosphorus and drought stress on some physiological characteristics of coriander (Coriandrum sativum L.), was conducted at Karaj Research Institute of Forest and Rangelands in 2006. Field experiment was carried out by a split factorial design with 4 replications. The factors were two level drought stress with irrigation after 30 mm water evaporation from evaporation pan (without stress conditions) and irrigation after 60 mm water evaporation (drought stress conditions) and also application and non-application of mycorrhiza, 0, 35 and 70 kgha-1 phosphorus fertilizer. The results showed drought stress has significant effect on water use efficiency, relative water content and proline accumulation rate (α=1%). Highest water use efficiency with 0.449 kgm-3 and proline accumulation rate with 6.767 mmol/ml were achieved under stress conditions and highest relative water content with %90.6 was achieved without stress conditions. Also, the results showed mycorrhiza and phosphorus had significant effects on water use efficiency (α=5%). Comparison of means showed that highest water use efficiency with 0.395 kgm-3 and 0.4 kgm-3 was achieved under application of mycorrhiza and application of 70 kgha-1 phosphorus, respectively. Relative water content and proline accumulation rate were not significantly affected due to phosphorus and mycorrhiza. The results showed that water use efficiency and proline accumulation rate were increased under drought stress conditions but relative water content decreased.
Z. Baher Nik; M.B. Rezaii; M. Ghorbanli; F. Asgari; M.K. Araghi
Volume 20, Issue 3 , October 2004, , Pages 263-275
Abstract
Because of the importance of water stress influence on plant growth, metabolism and yield, Satureja hortensis L. was imposed towater stress treaments. Irrigation treatments were selected basis on the different percentage of field capacity (FC), including (i) a control which was irrigated to full field ...
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Because of the importance of water stress influence on plant growth, metabolism and yield, Satureja hortensis L. was imposed towater stress treaments. Irrigation treatments were selected basis on the different percentage of field capacity (FC), including (i) a control which was irrigated to full field capacity during the growing season (FC), (ii) two moderate water stress treatments during vegetative (LS1) and flowering stages(LS2)and (iii) severe water stress treatments during flowering stages(HS). Results showed that plant water potential reduced from -0.5 to 1.5 Mpa and RWC (relative water content) decreased too. Also water stress induced high amount of proline. The amount of proline was the highest in sever treatment while it changed from 0.69 to 7.36 μ M/g FW in FC, LS2 treatments.
