S. Khalili; A. Moieni; M. Abdoli
Abstract
Echinacea is one of the most important medicinal plants and an important source of cichoric acid. In this research, callus induction, indirect shoot regeneration, and shoot rooting of Echinacea angustifolia DC. were investigated. The effects of explant (leaf, petiole and root) and NAA concentrations ...
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Echinacea is one of the most important medicinal plants and an important source of cichoric acid. In this research, callus induction, indirect shoot regeneration, and shoot rooting of Echinacea angustifolia DC. were investigated. The effects of explant (leaf, petiole and root) and NAA concentrations (0.5, 1 and 1.5 mg l-1) on callus induction as well as the effects of explant (leaf, petiole and root) and BAP concentrations (3, 4 and 5 mg l-1) on the indirect shoot regeneration of callus were evaluated in two separated experiments. The study was conducted as factorial in a completely randomized design (CRD). The effects of IBA concentrations (0.5, 1, 1.5 and 2 mg l-1) on shoot rooting were also investigated in a completely randomized design. The effects of the factors studied on the callogenesis were not significant while these factors had significant effects on the shoot regeneration and rooting at 1% probability level. In general, the results indicated that the highest indirect shoot regeneration (72%) was obtained from the leaf explant and by the use of 4 mgl-1 BAP. In addition, the highest shoot rooting (38% and 40%) was obtained using 1 and 1.5 mgl-1 IBA. The good potential of this species for indirect shoot regeneration was shown in this research. These results can be used in micropropagation programs, gene transfer, and transgenic plants production.
R. Shahhoseini; M. Moghaddam; D. Kiani; R. Mansori
Abstract
Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD ...
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Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD (Randomized Complete Block Design) with six treatments (0, 1000, 2000, 3000, 4000 and 5000 mg l-1) and four replications in order to evaluate the effects of different concentrations of IBA and NAA on rooting of semi-hardwood cuttings of rosemary. Cuttings were treated in solutions of IBA and NAA for 1 min and held in plastic tunnel under mist system. After 70 days, the percentage of rotting, number of roots, average root length, the root fresh and dry weight, length of cutting, leaves and stem fresh and dry weight were measured. The analysis of variance showed that application of IBA and NAA had a significant effect on all the parameters studied. The highest rooting percentage was obtained at concentration of 1000 mg l-1 NAA (84%) and 5000 mg l-1 IBA (66%). The concentration of 4000 mgl-1 IBA was the best treatment on quality and rooting of the semi-hardwood cuttings of rosemary.
F. Alavi Naeini; Z. Asrar; H. Mozafari
Abstract
Rosemary (Rosmarinus officinalis L.) is of great value as an ornamental and medicinal plant. Therefore, studying the growth, morphological and anatomical parameters of rosemary cuttings in rooting stage is of utmost importance. In recent research, the separate and combined effects ...
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Rosemary (Rosmarinus officinalis L.) is of great value as an ornamental and medicinal plant. Therefore, studying the growth, morphological and anatomical parameters of rosemary cuttings in rooting stage is of utmost importance. In recent research, the separate and combined effects of Indole 3- Butyric acid (IBA) and vitamin- B1 (thiamine) on growth, rooting and anatomical structure of adventitious roots in Rosmarinus officinalis cuttings were investigated under hydroponic conditions. A factorial experiment was implemented using nine combined treatments including IBA and vitamin B1 at three levels. The treatments were applied on cuttings for two weeks under standard condition of light, temperature and humidity in hydroponic medium. Then, using cross-section method and double staining, the anatomy of root, in terms of cross-section, cortex cells growth and the density of secondary roots, were studied by a light microscope. Results showed that IBA application with or without B1 significantly affected the growth, anatomy and morphology of rosemary cuttings in rooting stage. However, our results clearly showed that vitamin B1 had an essential role on the growth of roots and IBA stimulated cell division in pericycle, causing the stimulation of rooting response of cuttings. According to he obtained results, the combined use of IBA and vitamin B1 showed significant effect on the propagation of rosemary as an ornamental and medicinal plant; therefore, their application could be recommended in commercial and farm conditions, and even in the case of other ornamental plants having difficulty propagating.
A. Bidmeshki; M.J. Arvin; K. Maghsoudi
Abstract
Drought stress is the most important factor which negatively affects the plant growth and crop productivity in arid and semi-arid areas. The main problem which Iranian farmers are faced with cultivation of garlic (Allium sativum L.) is low production efficiency and performance. Therefore, in a field ...
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Drought stress is the most important factor which negatively affects the plant growth and crop productivity in arid and semi-arid areas. The main problem which Iranian farmers are faced with cultivation of garlic (Allium sativum L.) is low production efficiency and performance. Therefore, in a field experiment, the effect of IBA (0 and 100 ppm) was studied on growth parameters, bulb yield and allicin content of a local garlic cultivar under water deficit conditions (40% reduction of available water) in 2010 in Jiroft. The results showed that drought stress significantly reduced chlorophylls (a, b and total), caretenoids, anthocyanins, bulb and allicin yield, plant fresh weight, root fresh weight and also increased ion leakage. However, it had no significant effects on the percentage of allicin. In contrast, under non-stress conditions, IBA significantly improved most recorded parameters and reduced ion leakage and amount of anthocyanins while it had no effect on product quality and quantity under water deficit conditions. Compared with the controls, IBA increased plant fresh weight, bulb yield, and allicin respectively by 30%, 19% and 25%.
M.H. Assareh; Z. Abravesh; S.R. Tabaei Aghdaei
Abstract
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature ...
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Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature shrubs. The branches were laid in sand bed, soaked for 24 h by different concentrations of IBA and NAA hormones. The results showed that the rate of rooting was high in sand bedding material in autumn and winter. The highest rooting percentage was %100 with 50 mgl-1 concentration of IBA in autumn and winter seasons and the lowest of rooting percentage was %53.33 with 50 mgl-1 concentration of NAA in autumn season. The rooting branches were cut from the parent plants after 3 months and planted in pots containing soil: sand: peatmoss complex (1:1:1) in greenhouse conditions, survival percentage was 90%.
