Agriculture and horticulture
A. Rafati; N. Valizadeh; F. Sefidkon; Y. Imani; F. Noormand Moaied
Abstract
Nepeta crassifolia Boiss. & Buhse, from Lamiaceae family, is an aromatic species with medicinal properties which is endemic to Iran. The seeds and aerial parts of this plant were collected from six different regions of East Azerbaijan province. After drying, the plant aerial parts essential oil was ...
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Nepeta crassifolia Boiss. & Buhse, from Lamiaceae family, is an aromatic species with medicinal properties which is endemic to Iran. The seeds and aerial parts of this plant were collected from six different regions of East Azerbaijan province. After drying, the plant aerial parts essential oil was obtained by hydrodistillation and their compounds were identified by GC and GC/MS. The collected seeds, after producing greenhouse seedlings, were planted in the field in a randomized complete block design with three replications. In the second year after planting, the flowering shoots were harvested, dried, and distilled. The results showed that there was significant differences among essential oil percentage of natural habitat samples which the highest amount belonged to "Marand" region, but the farm essential oil samples did not differ with each other significantly. The compounds nepetalactone I (4aα, 7α, 7aα) and II (4aα, 7α, 7aβ), globulol, germacrene B, caryophyllene oxide, and 1,8-cineole were identified as the main essential oil compounds of this species. The highest amount of nepetalactone I or II under the natural habitat (69% and 21.7%, respectively) and field (27.3% and 51.5%, respectively) conditions belonged to "Misho" region in "Marand". "Sarab" region also had the highest amount of nepetalactone II under the natural habitat and farm conditions compared to the other samples. The highest amount of 1,8-cineole (21.8%) in the oil was also obtained from the natural habitat of "Kharil" region.
Agriculture and horticulture
M. Babaei; F. Sefidkon; M. Nasiri
Abstract
Two species Nepeta cataria L. and Nepeta bracteata Benth. (fam. lamiaceae) are native to Iran. To study the quantity and quality of the two species essential oil in the field, the seeds of eight populations from these two species were collected from natural habitats and planted in a randomized complete ...
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Two species Nepeta cataria L. and Nepeta bracteata Benth. (fam. lamiaceae) are native to Iran. To study the quantity and quality of the two species essential oil in the field, the seeds of eight populations from these two species were collected from natural habitats and planted in a randomized complete block design in Alborz Research Station of Research Institute of Forests and Rangelands (Karaj, Alborz province). The flowering branches were harvested at the full flowering stage and after room temperature-drying, their essential oils were extracted by hydrodistillation, and analyzed and identified by GC and GC/MS. The essential oil yield of N. cataria populations varied between 0.02 (Karaj) and 0.50% (Arak). Twenty-three compounds were identified in the essential oil of this species, and the main compound in all populations was from nepetalactone isomers. NepetalactoneIII (4aα,7β,7aα-nepetalactone) and nepetalactoneI (4aα,7α,7aα-nepetalactone) constituted 44.4 (Karaj) to 91.6% (Arak) and 0.8 (Karaj) to 15.9% (Bafgh1) of the essential oil, respectively. NepetalactoneII (4aα,7α,7aβ-nepetalactone) was observed only in the population Bafgh2 (21.2%). The amount of 1,8-cineole in the essential oil of different N. cataria populations varied from 0.4 (Taft1) to 12.8% (Karaj). The essential oil yield of N. bractaeta populations was obtained between 0.02 (Ardakan) and 0.70% (Taft2). Twenty-seven compounds were identified in the essential oil of this species, and the main compounds were 1,8-cineole (1.0, 9.6, and 41.0% in Tabas, Ardakan, and Taft2, respectively) and geranyl acetate (0.9, 3.4, and 39.8% in Tabas, Taft2, and Ardakan, respectively). In general, the results showed that the N. cataria populations were all from the same chemotype, but N. bracteata populations were from two chemotypes (ct. geranyl acetate and ct. 1,8-cineole).
B. Bahreininejad; B. Abaszadeh; F. Sefidkon; Z. Jaberalansar
Abstract
Two species of Nepeta assurgens Hausskn. & Bornm. and N. eremokosmos Rech., are endemic medicinal plants to Iran. This study was conducted to investigate the vegetative traits, aerial parts yield, the quantity and quality of essential oil, as well as evaluating the relationships between these traits ...
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Two species of Nepeta assurgens Hausskn. & Bornm. and N. eremokosmos Rech., are endemic medicinal plants to Iran. This study was conducted to investigate the vegetative traits, aerial parts yield, the quantity and quality of essential oil, as well as evaluating the relationships between these traits in mentioned species under field conditions in Isfahan. The experiment was carried out in a randomized complete block design with three replications over 2017 and 2018. Results showed that the fresh and dry weight of aerial parts in N. assurgens (11353 and 2351 kg ha-1, respectively) was remarkably higher than that of N. eremokosmos (2638 and 527 kg ha-1, respectively). The average content and yield of essential oil in N. assurgens and N. eremokosmos was 1.60 and 0.64%, and 20.16 and 2.21 kg ha-1, respectively. Based on qualitative analysis of essential oil, the total amount of nepetalactone isomers (4aα, 7α, 7aα-nepetalactone, 4aα, 7α, 7aβ-nepetalactone, 4aα, 7β, 7aα-nepetalactone) in N. assurgens and N. eremokosmos was 78.6 and 62.8% on average, respectively. Correlation analysis showed that leaf dry weight, total fresh and dry weight, and large diameter of the plant had the highest correlation with essential oil yield. Stepwise regression analysis indicated that essential oil yield changes were mainly explained by leaf dry weight, essential oil content, total fresh weight, the number of stems and the amount of plant crown cover. In general, according to the results, the superiority of the N. assurgens species was significant and could be recommended as a valuable and high potential yielding plant for pharmaceutical purposes.
Sh. Ahmadi; M. Mirza; F. Askari
Abstract
N. elymatica Bornm. is one of the endemic species in the genus Nepeta in Iran. This genus has important aromatic species. So far, 79 species have been reported from Iran, of which 39 species are endemic. The aim of this study was to identify the chemical composition of the essential oil of N. elymatica ...
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N. elymatica Bornm. is one of the endemic species in the genus Nepeta in Iran. This genus has important aromatic species. So far, 79 species have been reported from Iran, of which 39 species are endemic. The aim of this study was to identify the chemical composition of the essential oil of N. elymatica Bornm. The flowering branches of this species were collected in July from Oshterankuh, Kamandan region of Azna. After drying at room temperature, the flowering branches of the plant were subjected to hydro-distillation. The oil yield was calculated and the oil composition was identified by GC and GC/MS analysis and retention index. Results showed that the yield oil of this species was 0.95% w/w and 27 components were identified, among which 1,8-cineole (20.6%), 4a-a,7-a,7a-a-nepetalactone (17.5%), E-caryophyllene (15%) and linalool (5.8%), were the main compounds.
M. Layeghhaghighi; M. Hassanpour Asil; B. Abbaszadeh; F. Sefidkon; M. Matinizadeh
Abstract
About 67 species of the genus Nepeta have been found in Iran and Nepeta pogonosperma Jamzad is endemic to Iran. In order to investigate the effect of water deficit on Nepetapogonosperma, an experiment was conducted under field conditions in 2015, at the Alborz Research Station, Research Institute of ...
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About 67 species of the genus Nepeta have been found in Iran and Nepeta pogonosperma Jamzad is endemic to Iran. In order to investigate the effect of water deficit on Nepetapogonosperma, an experiment was conducted under field conditions in 2015, at the Alborz Research Station, Research Institute of Forests and Rangelands, Karaj, Iran. The experiment was conducted in a randomized complete blocks design with three replications. Treatments consisted of three levels: 30, 60, and 90% of field capacity. Harvesting was done in full flowering stage. Essential oil was extracted by distillation for 2 hours and 30 minutes. The percentage of essential oil components was determined using GC and GC/MS. In the Nepeta pogonosperma Jamzad essential oil, 16 components were identified and the highest percentage of 15 components including α-thujene, α-pinene, sabinene, β-pinene, myrcene, α-terpinene, ρ-cymene, e-β-ocimene, γ-terpinene, terpinen-4-ol, α-terpineol, 4aα,7α,7aα-nepetalactone, 4aα,7α,7aβ-nepetalactone, E-caryophyllene and β-bisabolene was related to the control treatment. Analysis of variance revealed that water deficit significantly affected the essential oil percentage, yield, and components detected. The highest essential oil percentage with an average of 3.18% belonged to the severe stress treatment (30% FC). The highest oil yield (78.321 kg per hectare) was recorded for the moderate stress treatment (60% FC). The highest content of 1.8-cineole belonged to 30% FC. The highest total content of nepetalactone with an average of 28.2% was obtained in the control group. Our results clearly showed that severe and moderate water deficit stress could be recommended for the production of plants with high essential oil content. However, no water deficit stress is recommended to obtain high content of nepetalactone.
N. Hadi; F. Sefidkon; A. Shojaeiyan; A.A. Jafari
Abstract
The genus Nepeta is one of the largest genera of the Lamiaceae family, and Iran, particularly, is one of the main centers of origin of this genus. Nepetalactones and flavonoids were reported as major constituents of Nepeta species, and the main cause of their medicinal value and biological properties. ...
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The genus Nepeta is one of the largest genera of the Lamiaceae family, and Iran, particularly, is one of the main centers of origin of this genus. Nepetalactones and flavonoids were reported as major constituents of Nepeta species, and the main cause of their medicinal value and biological properties. There are lots of reports related to biological activities of secondary metabolites of genus Nepeta showing the importance of this genus. In this work, the essential oil (EO) diversity of 21 wild populations from Nepeta kotschyi Boiss., Iranian endemic species, was investigated. For removing the environmental effect, the seeds of populations were planted in one place. Plant aerial parts were harvested at full flowering stage, and after shade-drying, their EO was extracted by hydrodistillation method. EO was quantitatively and qualitatively analyzed by GC and GC/MS. After botanical study and EO analysis, it was revealed that the populations were from two different varieties. Two populations including buyer-ahmad1 and Buyer-Ahmad2, were from N. kotschyi var. kotschyi, and others were stood in N. kotschyi var. persica. Twenty-four components were characterized in the EO of N. kotschyi. The highest amount of EO yield (w/w) was obtained in populations of var. kotschyi (0.5-0.7%). Three main chemotypes were identified among populations of var. persica based on the main component(s) of EO, including a containing NepI (4aα,7α,7aα-nepetalactone), b containing NepII (4aα,7α,7aβ-nepetalactone) and cubenol, and c containing geranyl acetate and cubenol. Except of semirom and Taft5 which were stood in b-chemotype, and Taft4 which was placed in c-chemotype, other populations of var. persica, also populations of var. kotschyi, were stood in a-chemotype. In addition, based on the main component of EO, the populations of var. kotschyi were put in a-chemotype. In a-chemotype, the amount of NepI was obtained between %53.9 (Chelgard) and %84.8 (Buyer-Ahmad2), and NepII was measured between %1 (Taft1) and %13.7 (Chelgard). In b-chemotype, the amount of NepI was measured between %0.3 (Taft5) and %4.9 (Semirom), and NepII was obtained between %13.4 (Taft5) and 44.7% (Semirom). NepIII (4aα,7β,7aα-nepetalactone) (1.3-3.3%) was characterized only in the EO of var. Kotschyi populations.
