B. Mosallanejad; R. Avizeh; H. Najafzadeh Varzi; M. Pourmehdi
Abstract
In the present study, effect of silymarin was evaluated as a protective drug of liver against acute hepatotoxicity due to administration of mebendazole in German shepherd dogs (mixed breeds). Twenty five healthy dogs were randomly allotted to five equal groups. Dogs in group A were given mebendazole ...
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In the present study, effect of silymarin was evaluated as a protective drug of liver against acute hepatotoxicity due to administration of mebendazole in German shepherd dogs (mixed breeds). Twenty five healthy dogs were randomly allotted to five equal groups. Dogs in group A were given mebendazole with single dose 150 mg⁄kg, p.o.; group B consisted of dogs that received silymarin with single dose 30 mg⁄kg, p.o. concurrent with mebendazole administration; groups C, D and E were treated like group B, but silymarin was administered 2, 12 and 24 h after administration of mebendazole respectively. The serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and total and direct bilirubin were measured before administration of mebendazole and 2, 12, 24 and 72 h later as indices of liver injury. A single oral administration of mebendazole significantly elevated serum concentrations of ALT, AST, ALP, LDH in all cases of group A (P<0.05), after 24 h. In both groups of B and C, levels of serumenzyme activities remained within the normal values. The difference was significant between groups B and C with group A (P<0.05). Levels of serumenzyme activities were higher than normal values in three cases of the group D and in all dogs of the group E. This study showed that silymarin could protect liver tissue against oxidative stress in dogs with mebendazole intoxication particularly in the first 2 hours after exposure.
L. Sadat Khorsandi; F. Javadnia; M. Orazizadeh; M. Abdolahi
Abstract
Regarding highly prescription of medicines, in recent years, herbal effects on prevention and treatment of toxicity induced by various drugs have received a great deal of attention. After water, tea is the most widely consumed beverage in the world. Green tea (Camellia sinensis L.) has antioxidant and ...
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Regarding highly prescription of medicines, in recent years, herbal effects on prevention and treatment of toxicity induced by various drugs have received a great deal of attention. After water, tea is the most widely consumed beverage in the world. Green tea (Camellia sinensis L.) has antioxidant and detoxification activities. In this study protective effect of green tea extract on hepaotoxicity induced by acetaminophen has been evaluated. 32 male mice were randomly divided into 4 groups. Control group received only normal saline. Green tea extract group was fed by 7mg/l green tea extract for 30 days. 500 mg/kg acetaminophen was administrated to acetaminophen group. Experimental group recieved 7mg/l green tea extract for 30 days and a single dose of 500 mg/kg acetaminophen prescribed in 29th day. In 31st day blood samples were taken from jugular arteries for biochemical tests. Then the liver placed in 10% formalin for histopathology assessments. ALT (Alanin Teransferase) and AST (Aspartate Teransferase) reduced significantly in experimental group (p<0.05). Histopathology assessments showed that necrosis of liver, congestion of blood cells and accumulation of inflammatory cells also reduced in experimental group compared to acetaminophen treated mice. These finding suggest that Green tea extract might have protective effect on liver damage induced by acetaminophen.