Biotechnology
B. Yousefi; S.R. Tabaei-Aghdaei
Abstract
To identify the appropriate detection methods of stable and adaptable genotypes for essential oil yield, an experiment was conducted on 35 Iranian Rosa damascena Mill. genotypes in a randomized complete block design with three replications in seven locations (Kurdistan, Markazi, Isfahan, Khuzestan, Fars, ...
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To identify the appropriate detection methods of stable and adaptable genotypes for essential oil yield, an experiment was conducted on 35 Iranian Rosa damascena Mill. genotypes in a randomized complete block design with three replications in seven locations (Kurdistan, Markazi, Isfahan, Khuzestan, Fars, Kerman, and Khorasan Razavi provinces) during two years (2007-2008). The total mean of essential oil yield was obtained 0.029%. There were significant differences (P≤0.01) between genotypes, locations, environments or location-years, and also the interactions of genotype × location and genotype × environment in terms of essential oil yield. The positive correlation between environmental variance, coefficient of variation, and regression coefficient of yield over environments with essential oil yield showed that only the low essential oil yield genotypes had the similar phenotype in different environments (static stability). There was no stable genotype with the static regression concept and the essential oil production of stable genotypes by environmental variance was very small, but some adaptable genotypes by the environmental coefficient of variation (e.g. KM1) had moderate essential oil yield and yield stability at the same time. The stable genotypes according to the dynamic stability concept had moderate essential oil yield. The superiority index introduced the genotypes with the highest essential oil as the adaptable ones. The stable genotypes with the lowest variance of the years within locations produced the least essential oil. The results showed that a R. damascena genotype can demonstrate the static and dynamic stabilities with the high essential oil yield at the same time. The coefficient of variation, dynamic aspect of regression statistics, and superiority index could be suggested as the desirable statistics to evaluate different aspects (static and dynamic) of essential oil stability in R. damascena. In general, the genotypes IS3, KZ1, and CM1 with the moderate essential oil yield and its general stability and adaptability could be recommended for Iran.
B. Yoosefi; H.R. Ghasempoor; B. Yousefi; S.R. Tabaeii Aghdaie; K. Jaimand
Abstract
Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of ...
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Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of this species and variations in the essential oil composition, the use of multivariate statistical analysis could be useful. In this study, 25 different accessions of Damask Rose were collected from Kermanshah province and other regions of Iran and were planted in a randomized complete blocks design. The essential oil was extracted from the petals with hydrodistillation method. Essential oil composition was identified by using the gas chromatography and gas chromatography coupled with mass spectrometry and the amount (percentage) of each composition was measured. Essential oil compositions were evaluated using cluster analysis. According to the results of cluster analysis, the accessions were grouped in four groups. Discriminant analysis confirmed the results of cluster analysis. Component analysis showed that the accessions were in four groups and there were four different trends among the accessions studied for the chemical compositions of essential oils. According to the correlation analysis, the two important factors, geraniol and citronellol, which increased the quality of essential oil compounds in damask rose, showed a significant positive relationship with each other and with citronellyl acetate and geranial.
