H. Najafzadeh; N. Erfanimajd; S. Gouraninejad; F. Haghighat
Abstract
Fertility and ovulation are affected by hormones and drugs. Hypericin is one of the drugs affecting the ovulation by neurotransmitters such as dopamine. Hypericin is an important component of Hypericum perforatom (S Johns Wot) used in treatment of depression and its important side effect is photosensitivity. ...
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Fertility and ovulation are affected by hormones and drugs. Hypericin is one of the drugs affecting the ovulation by neurotransmitters such as dopamine. Hypericin is an important component of Hypericum perforatom (S Johns Wot) used in treatment of depression and its important side effect is photosensitivity. In present study, the effect of hypericin on rat ovulation was evaluated. This study was carried as following on 30 female immature 25-day rats. At first day, PMSG was administrated to all rats at nineo’clock in the morning except control group. The control group was kept without drugs and under similar conditions with other groups. No drug was administrated on the second day of study. Positive control group (1) received gonadotropine at dose of 800 ng subcutaneously on day 3. Positive control group (2) received phenobarbital at dose of 4mg/kg interaperitoneally at one o’clock in the afternoon and positive control (3) received phenobarbital at dose of 4mg/kg interaperitoneally at one o’clock in the afternoon and GnRH at dose of 800 ng subcutaneously 2 hours later. Test group (1) was similar to positive control (3), in addition, hypericin was administrated orally at dose of 25mg/kg 30 min before phenobarbital. Test group (2) was similar to test group (1), but hypericin was given at dose of 50mg/kg. The rats were euthanized on day 4 of study at nine o’clock in the morning. The number of mature follicles, hyperemic follicles and corpus hemoragicum was counted. The results showed that administration of hypericin increased growth of follicles and number of mature follicles, hyperemic follicles, and corpus hemoragicum and it enhanced ovulation in comparison to control. However, a final comment on the mechanisms of hypericin effect needs the measurement of related hormones and neurotransmitters.
N. Moezzi; H. Najafzadeh Varzi; S. Shirali
Abstract
Decrease of collateral effect of wound repairing is one of the important objects of radiology and surgery studies. Identification of the effective, cheap and available drugs that have public grace is necessary. Whereas the Oleaster (Elaeagnus angustifolia L.) extract and Henna (Lowsonia intermis L.) ...
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Decrease of collateral effect of wound repairing is one of the important objects of radiology and surgery studies. Identification of the effective, cheap and available drugs that have public grace is necessary. Whereas the Oleaster (Elaeagnus angustifolia L.) extract and Henna (Lowsonia intermis L.) had anti-inflammatory effect in previous studies, so, in this study we compared their effect with silver sulfadiazine in wound healing. Female rats divided to four groups (four rats in each group). Rats anesthetized with standard combination of Ketamin-HCl and Acepromazine and incised with surgical blade on the mid dorsal line, then sutured with nylon suture. The wound was six centimeter length and five knobs with one centimeter distances applied. The wound length measured every day until complete recovery. Five hundred milligrams of oleaster extract, Henna paste and silver sulfadiazine ointment applied on wounds every day and one group didn't have any drugs as a control group. Average of repairing percent in first week in sulfadiazine and Oleaster groups were 90%, whereas this was 70% for henna group and 50% for control group. Complete wound healing in control groups; Henna, Oleaster and Sulfadiazine were observed in 13, 12, 10 and 8 days after surgery, consecutively. As a result, Olester extract can repair the wound same as silver sulfadiazine, whereas the Henna did not show the same effect in that definite time.
H. Morovvati; H. Najafzadeh; R. Avizeh; N. Khadivi Kashani
Volume 24, Issue 3 , November 2008, , Pages 363-373
Abstract
Drug-induced ototoxicity is one of the important factors for less auditory in dogs. Aminoglycosides including gentamicin can produce ototoxicity in dogs. The oxidative stress can be related to it. Silymarin, in the seed extract of Silybum marianum, is a potent antioxidant. In present study, the effect ...
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Drug-induced ototoxicity is one of the important factors for less auditory in dogs. Aminoglycosides including gentamicin can produce ototoxicity in dogs. The oxidative stress can be related to it. Silymarin, in the seed extract of Silybum marianum, is a potent antioxidant. In present study, the effect of silymarin and vitamin E was compared on gentamaicin-induced ototoxicity. The study was done on dogs in 5 groups: group1 was kept as control and received saline. Gentamicin was prescribed in other groups once for 9 days. Vitamin E and silymarin were given in group 3 and 4, respectively. In group 5, vitamin E and silymarin were co-administrated. After 9 days, the dogs were euthanized and ear samples were collected. One ear of dogs was colored by silver nitrate and another was decalcified by decalcificative solution. The decalcified samples were routinely sectioned and colored by Hematoxillin- Eosine (H&E) and studied by light microscope. Hair cells were not determined by silver nitrate. Organ of coarti body, tectorial membrane, limbus spiral, spiral ganglion, interdental cells, supporting cells and type I and II of hair cells were identified in samples and colored by H&E. These histological compartments were determined in control and silymarin groups. They were not observed in other groups, because gentamicin strongly destroyed cells. Thus, silymarin decreases gentamicin-induced changes in inner ear probably by its antioxidative property.
