Biotechnology
B. Yousefi; S.R. Tabaei-Aghdaei
Abstract
To identify the appropriate detection methods of stable and adaptable genotypes for essential oil yield, an experiment was conducted on 35 Iranian Rosa damascena Mill. genotypes in a randomized complete block design with three replications in seven locations (Kurdistan, Markazi, Isfahan, Khuzestan, Fars, ...
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To identify the appropriate detection methods of stable and adaptable genotypes for essential oil yield, an experiment was conducted on 35 Iranian Rosa damascena Mill. genotypes in a randomized complete block design with three replications in seven locations (Kurdistan, Markazi, Isfahan, Khuzestan, Fars, Kerman, and Khorasan Razavi provinces) during two years (2007-2008). The total mean of essential oil yield was obtained 0.029%. There were significant differences (P≤0.01) between genotypes, locations, environments or location-years, and also the interactions of genotype × location and genotype × environment in terms of essential oil yield. The positive correlation between environmental variance, coefficient of variation, and regression coefficient of yield over environments with essential oil yield showed that only the low essential oil yield genotypes had the similar phenotype in different environments (static stability). There was no stable genotype with the static regression concept and the essential oil production of stable genotypes by environmental variance was very small, but some adaptable genotypes by the environmental coefficient of variation (e.g. KM1) had moderate essential oil yield and yield stability at the same time. The stable genotypes according to the dynamic stability concept had moderate essential oil yield. The superiority index introduced the genotypes with the highest essential oil as the adaptable ones. The stable genotypes with the lowest variance of the years within locations produced the least essential oil. The results showed that a R. damascena genotype can demonstrate the static and dynamic stabilities with the high essential oil yield at the same time. The coefficient of variation, dynamic aspect of regression statistics, and superiority index could be suggested as the desirable statistics to evaluate different aspects (static and dynamic) of essential oil stability in R. damascena. In general, the genotypes IS3, KZ1, and CM1 with the moderate essential oil yield and its general stability and adaptability could be recommended for Iran.
S.R. Tabaei-Aghdaei; M. Abootorabi Najafabadi; M.H. Lebaschi; A. Najafi-Ashtiani; A.A. Jafari; F. Sefidkon; L. Mirjani
Abstract
To evaluate biomass and oil percentage at flowering stage in different accessions of medicinal plants Satureja spicigera (C. Koch) Boiss. (spic1, spic2, spic3)and S. sahendica Bornm. (sah1, sah2, sah3, sah4, sah5, sah6)under dry farming condition, an experiment was carried out based on three-replicated ...
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To evaluate biomass and oil percentage at flowering stage in different accessions of medicinal plants Satureja spicigera (C. Koch) Boiss. (spic1, spic2, spic3)and S. sahendica Bornm. (sah1, sah2, sah3, sah4, sah5, sah6)under dry farming condition, an experiment was carried out based on three-replicated randomized complete block design in Damavand, Iran in 2014. Variance analysis showed significant differences (P<0.01) between two species for all the traits with the higher values for S. spicigera. Based on means comparison, higher dry shoot weight per plant(108.5 g) was detected in S. spicigera compared to S. sahendica with 25.82g. The highest amount of dry shoot weight per plant was obtained from spic2 accession (183.5g) in S. spicigera and from sah2 (45.51 g) in S. sahendica.Also, S. spicigera showed higher oil content (2.65%) compared to S. sahendica with 1.34%. Spic2 with the highest amount of oil (2.90%, 36.10 kg/ha) could be the best accession for cultivation under dry farming condition of Damavand. Considering lower plant canopy diameter in S. sahendica (32.19 cm) compared to S. spicigera (54.90cm), biomass and oil yield increase is possible with higher plant density. Furthermore, considering dry shoot weigh, plant height, plant canopy and day to flowering as selection indices, sah2 could be proposed as superior genotype of S. sahendica for dry land farming in Damavand or similar climates.
A. Zarezadeh; F. Sefidkon; S.R. Tabaei Aghdaei; A. Mirhosseini; M.R. Arabzadeh; M.R. Mirjalili
Abstract
Essential oils of Satureja spp. contain valuable components, such as thymol and carvacrol. This research was carried due to evaluate essential oil quality and quantity of different accessions of Satureja species in cultivated condition., Seeds of 35 accessions of 10 Satureja species were collected from ...
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Essential oils of Satureja spp. contain valuable components, such as thymol and carvacrol. This research was carried due to evaluate essential oil quality and quantity of different accessions of Satureja species in cultivated condition., Seeds of 35 accessions of 10 Satureja species were collected from natural habitats and the seedlings were planted, using a randomized complete block design with for three replications at Medicinal Plants Research Station, Yazd, Iran. Aerial parts of plants were collected during four consecutive years, air dried in shadow and essential oils were extracted with hydrodistillation clevenger method. Essential oil compounds were identified by Gas chromatography (GC) and chromatography-mass spectroscopy (GC/MS). Analysis of variance showed significant differences at 1% between accessions for percentage of essential oil and essential oil yield per hectare. According to the results, accessions 15 (Satureja rechingeri) from Ilam province with 5.8% and 113.9 kg/ha essential oils, and 64% carvacrol and 12.2% thymol; accession 107 (S. spicigera) from Gillan province with 2.4% and 75.5 kg/ha essential oils, and 9.2% carvacrol and 43.4% thymol; accession 24 (S. rechingeri) from Ilam province with 4.2% and 63.8 kg/ha essential oils, and 75.4% carvacrol and 6.2% thymol; and SKM (S. bachtiarica ) from Yazd province with 2.6% and 51.5 kg/ha, essential oils, and 66% carvacrol and 0.5% thymol, respectively, showed the highest essential oil production in Yazd, Iran.
A. Zarezadeh; S.R. Tabaei aghdaei; A. Mirhosseini; M.R. Arabzadeh; L. Mirjani
Abstract
Satureja belongs to lamiaceae with different species throughout the world. Annual and perennial species of this medicinal plant grow naturally in Iran, of which nine species are endemic. In this research, perennial species of Iranian savory were evaluated during 2009-2013, Yazd, Iran. Accessions of different ...
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Satureja belongs to lamiaceae with different species throughout the world. Annual and perennial species of this medicinal plant grow naturally in Iran, of which nine species are endemic. In this research, perennial species of Iranian savory were evaluated during 2009-2013, Yazd, Iran. Accessions of different species were cultivated at Research Station of Medicinal Plants using a randomized complete block design. Different traits including percentage of plant establishment, plant height, plant canopy diameter, shoot fresh yield, shoot dry yield and oil content were measured. Variance analysis showed significant differences (p<0.01) among accessions for plant establishment, plant canopy diameter, plant height, essential oil percentage, shoot dry yield and leaf dry yield . Based on mean comparisons, SKM (Satureja bachtiarica) from Yazd, 107 (S. spicigera) from Gilan, 15 (S. rechingeri) from Ilam, 123-1 and 123-2 (S. mutica) from Khorasan province were superior accessions for valuable agronomic traits such as percentage of establishment, shoot yield and oil content.
E. Alimardan; P. Salehi Shanjani; A.A. Jafari; S.R. Tabaei Aghdaei
Abstract
Achillea L. is one of the most important native medicinal genuses of Iran. Morphological and agronomic traits in 27 populations of A. millefolium and in 14 populations of A. bieberestini were studied using a random complete blocks design with three replications in Alborz Research Center, Karaj, ...
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Achillea L. is one of the most important native medicinal genuses of Iran. Morphological and agronomic traits in 27 populations of A. millefolium and in 14 populations of A. bieberestini were studied using a random complete blocks design with three replications in Alborz Research Center, Karaj, Iran. ANOVA suggested significant differences among 27 populations of A. millefolium for all morphological and agronomic traits except full flowering time. Significant differences were found among 14 populations of A. bieberestini for all morphological and agronomic traits. In both species of A. millefolium and A. bieberestini significant correlation coefficient were found between the plant height and the flower diameter. Comparison of dry matter yield and other characteristics of different populations indicated that populations with dry and hot origin in both species of A. millefoliuum and A. bieberestini had better productivity. Population Semnan (21657) in A. millefoliuum and population Park golestan in A. bieberestini showed better characteristics in plant height, plant diameter, main inflorescence diameter, dry matter yield and inflorescence number as compared with other populations, which could be suggested for breeding programs.
K. Jaimand; M.B. Rezaee; S.R. Tabaei Aghdaei; M. Nadery Hajibagher Kandy; S. Meshkizadeh
Abstract
In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, ...
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In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, rose water, wastewater and petal residue were prepared. The amount of tannin in the samples was measured by colorimetric method and spectrophotometer apparatus (Model 340 Hitachi) at 760 nm. The highest amount of tannin in rose water, wastewater and petal residue was respectively obtained from Khuzistan and Ilam (2163 ppm), Qom (1846 ppm), and Gillan (1432 ppm) accessions for rose water, Zanjan (1919 ppm), Mazandaran (1895 ppm) Qom, Gilan, Lorestan and Isfahan (1871 ppm) accessions for wastewater, and Yazd (3432 ppm), Sistan and Baluchestan (3139 ppm) and Kohgiluyeh-Boyer Ahmad (2993 ppm) accessions for petal residual. According to the results, it is recommended to extract tannin from samples used in rose water production. In other words, this will be economically useful for rose water traditional producers.
A. Soleimani; H. Lessani; S.R. Tabaei-Agdaei
Abstract
The experiment was conducted in 1380-1381, on one year old olive plant cvs; "Zard", "Roghani" (native cvs.) and "Mission", "Koroneiki" (foreign cvs.) during two different growth status; cold and non-cold acclimated phase. Natural cold treatment was achieved by exposure plant to low temperature during ...
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The experiment was conducted in 1380-1381, on one year old olive plant cvs; "Zard", "Roghani" (native cvs.) and "Mission", "Koroneiki" (foreign cvs.) during two different growth status; cold and non-cold acclimated phase. Natural cold treatment was achieved by exposure plant to low temperature during fall and winter in 1381. the influence of cold stress on plant growth, wet and dry weight, shoot tip burn and water content were studied. In order to determine ionic leakage leaf tissues were exposure to artificial freezing temperature; 0, -4, -8 and -12°C ranged by 4°Cper hour. There was significant difference between cultivars with regards to vegetative growth index. The cv. "Koronieki" with more shoot growth, showed the more shoot tip burn damage. Based on ionic leakage study at non-acclimated phase, Olive cvs. were classified in distinct classes, includes: cold tolerance, semi tolerance and cold sensitive. However use of lower artificial freezing temperature especially during cold acclimated status will provide more reliable and certain results.
