S.R. Tabaei-Aghdaei; M. Abootorabi Najafabadi; M.H. Lebaschi; A. Najafi-Ashtiani; A.A. Jafari; F. Sefidkon; L. Mirjani
Abstract
To evaluate biomass and oil percentage at flowering stage in different accessions of medicinal plants Satureja spicigera (C. Koch) Boiss. (spic1, spic2, spic3)and S. sahendica Bornm. (sah1, sah2, sah3, sah4, sah5, sah6)under dry farming condition, an experiment was carried out based on three-replicated ...
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To evaluate biomass and oil percentage at flowering stage in different accessions of medicinal plants Satureja spicigera (C. Koch) Boiss. (spic1, spic2, spic3)and S. sahendica Bornm. (sah1, sah2, sah3, sah4, sah5, sah6)under dry farming condition, an experiment was carried out based on three-replicated randomized complete block design in Damavand, Iran in 2014. Variance analysis showed significant differences (P<0.01) between two species for all the traits with the higher values for S. spicigera. Based on means comparison, higher dry shoot weight per plant(108.5 g) was detected in S. spicigera compared to S. sahendica with 25.82g. The highest amount of dry shoot weight per plant was obtained from spic2 accession (183.5g) in S. spicigera and from sah2 (45.51 g) in S. sahendica.Also, S. spicigera showed higher oil content (2.65%) compared to S. sahendica with 1.34%. Spic2 with the highest amount of oil (2.90%, 36.10 kg/ha) could be the best accession for cultivation under dry farming condition of Damavand. Considering lower plant canopy diameter in S. sahendica (32.19 cm) compared to S. spicigera (54.90cm), biomass and oil yield increase is possible with higher plant density. Furthermore, considering dry shoot weigh, plant height, plant canopy and day to flowering as selection indices, sah2 could be proposed as superior genotype of S. sahendica for dry land farming in Damavand or similar climates.
M. Mottaghi; P. Salehi Shanjani; A.A. Jafari; M. Mirza; M.R. Bihamta
Abstract
Molecular, morphological and phenological markers were used to detect genetic diversity in several populations of Anthemis haussknechtii' Boiss. & Reut. growing in six different provinces in center and west of Iran. Variance analysis of morphologicalandphenological traits showed that all traits were ...
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Molecular, morphological and phenological markers were used to detect genetic diversity in several populations of Anthemis haussknechtii' Boiss. & Reut. growing in six different provinces in center and west of Iran. Variance analysis of morphologicalandphenological traits showed that all traits were significantly different among populations. Essential oil percentage, number of flowering stems and flower diameter showed the highest coefficient of variation (45.76, 38.76 and 32.86%, respectively). Six ISSR primers revealed 55 polymorphic bands, of which 38 (69.1%) were polymorphic. The ranges of distance between populations were varied from 0.009 to 0.439. According to the derived dendrogram from UPGMA, at a similarity level of 0.66, the populations were divided into two main groups. Grouping populations based on molecular markers was in accordance with geographical grouping. Among the populations, Ilam population is recommended for tropical areas with proper irrigation conditions, Ardakan and Khorramabad populations for commercial operators and research centers and Maybod population for cultivation in arid and semi-arid regions. Wide domain of genetic variation in this study could be considered as an available gene pool for A. haussknechtii improvement through selection and hybridization programs. Therefore, conservation strategies should be provided to maintain such diversity to apply in future breeding programs.
F. Shahbazi Asl; A.A. Jafari; H. Panahpour
Abstract
In order to study the biomass yield in flowering stage and morphological traits in Mentha spp., 17 accessions of M. pulegium L. and 8 accessions of M. mozaffariani Jamzad were assessed in a field experiment over two years in the Alborz research station, Karaj Iran. The accessions were planted using ...
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In order to study the biomass yield in flowering stage and morphological traits in Mentha spp., 17 accessions of M. pulegium L. and 8 accessions of M. mozaffariani Jamzad were assessed in a field experiment over two years in the Alborz research station, Karaj Iran. The accessions were planted using a complete randomized blocks design in two replications. Each plot contained 15 spaced plants with 0.5 m intervals and 75 cm between rows. The drip irrigation system was used. Data were collected for fresh and dry matter yield over two years and plant height, stem number, number of flowers per plant, the canopy area, and flower length for one year. Data were analyzed, and species means were compared based on DMRT Method. The results showed significant difference between the species for all traits except dry matter yield (P<0.01). In comparisons between species, M. pulegium had higher values of all traits except flower length than that for M. mozaffariani. For fresh yield, M. pulegium with an average value of 183 g/plant had significantly higher production as compared with M. mozaffariani with an average value of 147 g/plant. There were significant differences among accessions for all traits in both species (P<0.01). In comparisons among the accessions of M. pulegium, the accessions Rafsanjan (23031) and Maryvan (22607) with the average of 252 and 231 g/plant had higher fresh yield over two years, respectively. For dry mater yield, Rafsanjan (23031) and Arak (22381) with average values of 115 and 111 g/plant had higher dry matter production, respectively. In comparisons among the accessions of M. mozaffariani, Urmia (19625) with average values of 202 and 98 g/plant had higher fresh and dry matter production, respectively. For essential oil percentage, there were no significant differences between two species; however, there was a significant difference for oil yield. M. pulegium with an average value of 1.14 g/plant had higher oil production than that for M. mozaffariani with an average value of 0.76 g/plant.
M. Mahmoodi Sourestani; M. Derikvandi; M. Chehrazi; A.A. Jafari
Abstract
In order to evaluate the compatibility of some borage (Borago officinalis L.) populations and cultivars to Ahvaz weather condition, an experiment was carried out based on a randomized complete block design with eight treatments and three replications. The treatments were six populations (Ahvaz, ...
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In order to evaluate the compatibility of some borage (Borago officinalis L.) populations and cultivars to Ahvaz weather condition, an experiment was carried out based on a randomized complete block design with eight treatments and three replications. The treatments were six populations (Ahvaz, Mashad, Andimeshk, Ghazvin, Esfehan and Mazandaran) and two cultivars (F1 and F2). Plant height, number of branches, leaf number, leaf area, fresh and dry weight of plants were measured at the full flowering stage. The one-thousand seed weight, seed and biological yields were measured at the end of the growing period. The seed oil was extracted by Soxhelt system and analyzed by gas chromatoghraphy (GC). Results showed that there was a significant difference between populations and cultivars for all morphological traits (p≥0.01). The highest and lowest amounts of leaf number, leaf area, fresh and dry weight of plant, biological yield, and oil content were obtained in F1 and Esfehan population, respectively. Palmitic, stearic, oleic, linoleic, α-linolenic,γ-linolenic, arachidonic and erucicacids were detected in all populations and cultivars. The highest (43.41%) and lowest (17.21%) amounts of linoleic acid were observed in Andimeshk and Mashad, respectively. Mashad population had the maximum amount (60.54%) of oleic acid. The lowest amount of oleic acid (18.49%) was recorded in Andimeshk. The highest (15.25%) and lowest (7.96%) amounts of γ-linolenic acid were observed in F1 and Mashad, respectively. Mazandaran and Mashad populations had the maximum and minimum amounts of α-linolenic acid. The highest amount of palmitic and stearic acids was observed in Esfahan and Mazandaran populations, respectively. Mashad had the lowest amount of palmitic and stearic acids. According to the obtained results, further researches are recommended to increase the F1 cultivation inside Khuzestan province.
N. Hadi; F. Sefidkon; A. Shojaeiyan; A.A. Jafari
Abstract
The genus Nepeta is one of the largest genera of the Lamiaceae family, and Iran, particularly, is one of the main centers of origin of this genus. Nepetalactones and flavonoids were reported as major constituents of Nepeta species, and the main cause of their medicinal value and biological properties. ...
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The genus Nepeta is one of the largest genera of the Lamiaceae family, and Iran, particularly, is one of the main centers of origin of this genus. Nepetalactones and flavonoids were reported as major constituents of Nepeta species, and the main cause of their medicinal value and biological properties. There are lots of reports related to biological activities of secondary metabolites of genus Nepeta showing the importance of this genus. In this work, the essential oil (EO) diversity of 21 wild populations from Nepeta kotschyi Boiss., Iranian endemic species, was investigated. For removing the environmental effect, the seeds of populations were planted in one place. Plant aerial parts were harvested at full flowering stage, and after shade-drying, their EO was extracted by hydrodistillation method. EO was quantitatively and qualitatively analyzed by GC and GC/MS. After botanical study and EO analysis, it was revealed that the populations were from two different varieties. Two populations including buyer-ahmad1 and Buyer-Ahmad2, were from N. kotschyi var. kotschyi, and others were stood in N. kotschyi var. persica. Twenty-four components were characterized in the EO of N. kotschyi. The highest amount of EO yield (w/w) was obtained in populations of var. kotschyi (0.5-0.7%). Three main chemotypes were identified among populations of var. persica based on the main component(s) of EO, including a containing NepI (4aα,7α,7aα-nepetalactone), b containing NepII (4aα,7α,7aβ-nepetalactone) and cubenol, and c containing geranyl acetate and cubenol. Except of semirom and Taft5 which were stood in b-chemotype, and Taft4 which was placed in c-chemotype, other populations of var. persica, also populations of var. kotschyi, were stood in a-chemotype. In addition, based on the main component of EO, the populations of var. kotschyi were put in a-chemotype. In a-chemotype, the amount of NepI was obtained between %53.9 (Chelgard) and %84.8 (Buyer-Ahmad2), and NepII was measured between %1 (Taft1) and %13.7 (Chelgard). In b-chemotype, the amount of NepI was measured between %0.3 (Taft5) and %4.9 (Semirom), and NepII was obtained between %13.4 (Taft5) and 44.7% (Semirom). NepIII (4aα,7β,7aα-nepetalactone) (1.3-3.3%) was characterized only in the EO of var. Kotschyi populations.
E. Alimardan; P. Salehi Shanjani; A.A. Jafari; S.R. Tabaei Aghdaei
Abstract
Achillea L. is one of the most important native medicinal genuses of Iran. Morphological and agronomic traits in 27 populations of A. millefolium and in 14 populations of A. bieberestini were studied using a random complete blocks design with three replications in Alborz Research Center, Karaj, ...
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Achillea L. is one of the most important native medicinal genuses of Iran. Morphological and agronomic traits in 27 populations of A. millefolium and in 14 populations of A. bieberestini were studied using a random complete blocks design with three replications in Alborz Research Center, Karaj, Iran. ANOVA suggested significant differences among 27 populations of A. millefolium for all morphological and agronomic traits except full flowering time. Significant differences were found among 14 populations of A. bieberestini for all morphological and agronomic traits. In both species of A. millefolium and A. bieberestini significant correlation coefficient were found between the plant height and the flower diameter. Comparison of dry matter yield and other characteristics of different populations indicated that populations with dry and hot origin in both species of A. millefoliuum and A. bieberestini had better productivity. Population Semnan (21657) in A. millefoliuum and population Park golestan in A. bieberestini showed better characteristics in plant height, plant diameter, main inflorescence diameter, dry matter yield and inflorescence number as compared with other populations, which could be suggested for breeding programs.
A.A Hossienpour Ggazviniy; M.A. Alizadeh; A.A. Jafari; A.R. Valadabadi
Abstract
In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised ...
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In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised design with three replications were used. The different treatments including: cold stratification, after-ripening, physical scarification (sand paper), chemical scarification (Ethylic alcohol 70%) and control were used for dormancy breaking. The treated seeds were sown in 3 petri dishes as replications. Then, all samples were placed in a germinator with condition of 20°C and light-to-dark cycle of 16 hours light (1000 lux) to 8 hours dark. The germination characteristics including: germination percentage, speed of germination, length of root and shoot, vigour index, fresh weight and dry weight were evaluated during 18 days of the experiment. There were significant differences amoung species for all seed germination characteristics. The ecotype of Khuzistan from S. khuzistanica had more seed germination and vigour compared to the other three species. The seed characteristics specially speed of germination and vigour index of ecotypes from cold zone were affected by cold treatment more than the other treatments.
Z. Nemati Lafmajani; S.R. Tabaei-Aghdaei; M.H. Lebaschi; A.A. Jafari; A. Najafi Ashtiani; M. Daneshkhah
Abstract
In this research, effects of different traits on flower yield of 12 Rosa damascena Mill. accessions from different regions of Iran were analyzed under irrigation (control) and non-irrigation with and without mulch conditions. Randomized complete block designs were applied with three replications at Research ...
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In this research, effects of different traits on flower yield of 12 Rosa damascena Mill. accessions from different regions of Iran were analyzed under irrigation (control) and non-irrigation with and without mulch conditions. Randomized complete block designs were applied with three replications at Research Station of Homand Absard, Damavand, Iran. Flower weight, flower number, flower dry matter percentage, oil percentage, oil yield (per ha), petal weight, plant height and canopy cover were recorded. According to the correlation analysis, flower yield positively correlated with petal weight under non-irrigation with mulch, irrigated (control) and non-irrigation without mulch conditions. The statistical multivariate methods were also applied included multivariate linear regression and path analyses. Using three separate stepwise-regression analysis made it possible to determine the traits with most important impacts on the flower yield, under irrigation (control) and non-irrigation with and without mulch conditions. Flower number, petal weight, oil yield, and oil percentage under non-irrigation without mulch totally explained 91.7% of the variation among genotypes in the regression model. Petal weight, plant cover, and diameter of the species under irrigation (control) totally explained 98.6% of the variation among genotypes in the regression model. Oil percentage, oil yield, petal weight, plant height and flower number per ha under non-irrigation with mulch totally explained 90.6% of the variation among genotypes in the regression model. The results indicated that petal weight has the most direct impact on flower yield based on the path analysis and oil yield showed a negative direct effect on the flower yield under non-irrigation (without mulch) condition. Oil percentage, oil yield, petal weight, plant height and flower number per plant under non-irrigation with mulch, irrigated (control) and non-irrigation without mulch totally explained 95/5% of the variation among genotypes in the regression model. Petal weight had the highest direct impact on flower yield based on the path analysis under irrigation and non-irrigation conditions. Therefore, it is possible to consider petal weight as a magnificent character to improve flower yield of Rosa damascene.
S.R Tabaei-Aghdaei; S. Farhangian; A.A. Jafari; M.B. Rezaee
Abstract
To evaluate 17 Damask rose genotypes regarding their variation for morphological traits, an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Analysis of variance, mean comparison and phenotypic correlation analysis were performed. The variance analysis ...
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To evaluate 17 Damask rose genotypes regarding their variation for morphological traits, an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Analysis of variance, mean comparison and phenotypic correlation analysis were performed. The variance analysis showed significant differences among genotypes for plant height and flower number per branch (P<0.01), number of leaves per branch, leaflet length and thorn density (P<0.05). Also, comparison of means classified the genotypes for the above characteristics. Significant correlations were observed between different traits. A significant (P<0.01) phenotypic correlation (r=0.96) indicated a strong positive relationship between flower yield and number of flower per plant. From the results, a wide range of variation was observed among the genotypes. Morphological characteristics could, therefore, be useful criteria for selection for yield. However, more genotypes, as well as further analyses, including flower quality especially essential oil components are necessary to be considered, in an efficient breeding of Rosa damascena.
S.R. Tabaei-Aghdaei; M. Sahebi; A.A. Jafari; M.B. Rezaee
Volume 20, Issue 2 , July 2004, , Pages 199-211
Abstract
In order to study the genetic variation in Rosa damascena Mill., an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Eleven genotypes were evaluated, using a randomized complete blocks design with three replications. Flower yield, flower number, ...
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In order to study the genetic variation in Rosa damascena Mill., an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Eleven genotypes were evaluated, using a randomized complete blocks design with three replications. Flower yield, flower number, single flower weight, plant height, canopy, branch angel, leaf number, spine density and spine length, were analyzed, using analysis of variance, principal components analysis and cluster analysis. The results showed significant differences among genotypes for all of the traits, indicating a considerable genetic variation in germplasm available in Iran. Using principal components analysis, the first three components determined 68.43% of the total variation. According to the cluster analysis, genotypes could be grouped into 3 clusters. Genotypes in cluster 1 averaged well above the overall mean for productivity and the other characteristics.
