A. Zarezadeh; A. Mirhossaini; M. Mirza; Z. Jamzad; M. Arabzadeh
Abstract
Thymuskotschyanus Boiss. & Hohen. is a perennial medicinal shrub growing wild in some regions of Iran including Yazd province. This research was carried out in order to analyze oil quantity and quality of cultivated Thymuskotschyanus in the Medicinal Plants Farm, Yazd, Iran. The seeds of 19 accessions ...
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Thymuskotschyanus Boiss. & Hohen. is a perennial medicinal shrub growing wild in some regions of Iran including Yazd province. This research was carried out in order to analyze oil quantity and quality of cultivated Thymuskotschyanus in the Medicinal Plants Farm, Yazd, Iran. The seeds of 19 accessions collected from natural habitats were sown in a greenhouse and the seedlings were planted in the farm. Shoots were harvested at 50% flowering stage in the second year and dried under shadow. Essential oil was extracted with water distillation method and oil components were identified after determining oil content. The highest amounts of oil content respectively were related to accessions TK7 (3.42%), TK12 (3.22%), TK17 (2.66%) and the lowest amount was observed in accession TK6 (0.79%). The highest amounts of oil production respectively were recorded in accessions TK5 (38.3kg/ha), TK7 (36.4kg/ha) and TK12 (36.1 kg/ha) and the lowest amount was related to TK1 (1.3 kg/ha). Cluster analysis based on eleven oil components classified the accessions into three groups. The first group, (TK2, TK6, TK13, TK18, TK16, TK19), was rich for thymol-p-cymene, the scond group (TK1, TK3, TK4, TK5, TK7, TK11, TK14, TK15, TK17) for carvacrol-thymol and the 3rd group (TK8, TK9, TK10, TK12) was rich for Geraniol-linalool. In general, accessions TK7, with 3.42% oil content, 36.4 kg/ha oil production and 63.8% carvacrol; TK14, with 2.2% oil content 35.5 kg/ha oil production and 47.2% carvacrol and thymol, TK5 with 2.4% oil content, 38.3 kg/ha oil production and 57% thymol and carvacrol are proposed as superior accessions.
R. Kalvandi; M. Mirza; M. Atri; M. Hesamzadeh Hejazi; Z. Jamzad; K. Safikhani
Abstract
Thymus eriocalyx (Ronniger) Jalas belongs to the family Lamiaceae. Among different species of Thymus, distributed in the world, 18 species are growing in Iran, of which four species are endemic. To study the variation of essential oils among population individuals of this species in Iran D.S.S. (Determination ...
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Thymus eriocalyx (Ronniger) Jalas belongs to the family Lamiaceae. Among different species of Thymus, distributed in the world, 18 species are growing in Iran, of which four species are endemic. To study the variation of essential oils among population individuals of this species in Iran D.S.S. (Determination of special station) method was used. In this study, the aerial parts of 5 individuals of Thymus eriocalyx were collected from 10 special stations, at full flowering stage for chemical investigation and were dried at room temperature. The essential oil was extracted by hydrodistilation method using a Clevenger-type apparatus. The chemical composition of essential oils was analyzed by GC-FID and GC-MS. Results showed that the highest average yield of essential oil in three replications based on dry weight belonged to the first individual of Te2 population (4.09%), while the lowest was recorded for the second individual of Te10 population (0.21%). Totally, thirty one compounds were identified in the essential oil of 10 studied population individuals. In this study, it is noteworthy that the individuals of a population showed variation among themselves in terms of chemical compositions. Among existing chemical compounds in the essential oil of 50 individuals, the following compounds revealed the variation widely: p-cymene (0.1-20.1%), 1,8-cineole (0.4-29.4%), γ-terpinene (0.1-8.7%), linalool (0.1-82.3%), camphor (0.1-15%), borneol (1.6-22.7%), geraniol (0.1-74.6%), thymol (0.1-57.7%), geranyl acetate (0.1-49.6%), caryophyllene oxide (0.2-9.1%), limonene (0.2-24%) and α-terpineol (0.1-36.2%). The results of the analysis of chemical data using MVSP Software by UPGMA and PCO methods led to the identification of 9 chemotypes as follows of which 7 chemotypes were introduced as new chemotypes: 1-geraniol/geranyl acetate, 2-geraniol, 3- linalool/geraniol/geranyl acetate, 4-linalool,5-α-terpineol/1,8-cineole, 6-geranyl acetate, 7-geraniol/thymol/borneol/1,8-cineole, 8-thymol/limonene and 9-thymol/p-cymene/borneol.
R. Kalvandi; S.M. Hesamzadeh Hejazi; M. Mirza; M. Atri; Z. Jamzad; K. Safikhani; M. Ahmadian
Abstract
In order to evaluate several ecological factors, morphological traits, essential oil productivity and ploidy levels of Thymus eriocalyx (Ronniger) Jalas with uses of DSS method ten natural habitats were selected in Lorestan, Markazi, Hamadan, Kermanshah and Kurdestan provinces in Iran. To ...
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In order to evaluate several ecological factors, morphological traits, essential oil productivity and ploidy levels of Thymus eriocalyx (Ronniger) Jalas with uses of DSS method ten natural habitats were selected in Lorestan, Markazi, Hamadan, Kermanshah and Kurdestan provinces in Iran. To study and evaluate morphological characters, five complete plant samples and flowering branches were collected from each locality at flowering stage for essential oil extraction in spring season of 2008. Ecological data of ten localities were also recorded. Vegetative and reproductive traits and essential oil quantity of each population were studied. Seeds were also collected in the seed production season to study seed characters, chromosome counting and ploidy level. At least 10 mitotic cells of each population were studied to determine chromosome numbers and ploidy levels. All populations were classified in 4 groups. Results revealed that specimens from "Te9 population" had the highest average length and width of inflorescence leaf, inflorescence leaf length to width ratio, stem leaf length to width ratio, petiole length, calyx tube length, corolla length and the highest average length and width of bracteole. The results of chromosome counting showed that specimens of Te1, Te2, Te3, Te4, Te5, Te6 and Te8 populations were diploid (2n=2x=30) and specimens of Te7, Te9 and Te10 populations were tetraploid (2n=4x=60). The highest essential oil production belonged to Te5 population (3.04%).
E. Modiri; F. Sefidkon; Z. Jamzad; A. Tavasoli
Abstract
The genus Ziziphora (Lamiaceae) consists of four species (Z. clinopodioides L., Z. capitata L., Z. persica Bunge. and Z. tenuior L.), wildly distributed in Iran. Ziziphora clinopodioides with the common Persian name of “kakuti-e kuhi” is a rich source of essential oils. In this study, aerial ...
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The genus Ziziphora (Lamiaceae) consists of four species (Z. clinopodioides L., Z. capitata L., Z. persica Bunge. and Z. tenuior L.), wildly distributed in Iran. Ziziphora clinopodioides with the common Persian name of “kakuti-e kuhi” is a rich source of essential oils. In this study, aerial parts of five subspecies of Z. clinopodioides were collected. After drying the plants, the essential oils were obtained by hydro-distillation. The composition of the essential oils was analyzed and identified by gas chromatography (GC) and gas chromatography/Mass (GC/MS). The essential oils of different subspecies were different qualitatively and quantitatively. In the essential oil of subsp. Rigida, collected from Urmia and Fars, 15 and 19 compounds were identified, respectively. In both, the main compound was pulegone (46.8%) and (36.1%), respectively. In the essential oil of subsp. filicaulis from Arasbaran, subsp. bungeana from Tabriz and subsp. ronnigeri from Urmia, 15, 18 and 19 compounds were identified, respectively. The main compound was pulegone (46%), (23%) and (52%), respectively. In the essential oil of subsp. pseudodasyantha, collected from Qazvin (before to Zoshk) and Qazvin, 14 and 16 compounds were identified, respectively. Pulegone (49.8%) and Limonene (29%) were identified as the main compounds, respectively. In the essential oil of the mentioned subspecies, collected from Garmabdareh and Shahrestanak, 18 and 16 compounds were identified, respectively. The main compounds were g-terpinene (16.1%) and 1,8-cineol (32.4%), respectively.
S. Shahverdi; F. Sefidkon; Z. Jamzad; T. Nejhadsattari
Abstract
Origanum strobilaceum Mobayen & Ghahreman belongs to Lamiaceae. Thirty eight species of the genus were known so far which are categorized in 9 sections. The species is aromatic and its essential oil compositions are valuable. In this research, for the first time, the essential oil content and composition ...
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Origanum strobilaceum Mobayen & Ghahreman belongs to Lamiaceae. Thirty eight species of the genus were known so far which are categorized in 9 sections. The species is aromatic and its essential oil compositions are valuable. In this research, for the first time, the essential oil content and composition of O. strobilaceum was studied. The aerial parts of the plant were collected from janat rodbar in Mazandaran Province road at full flowering stage. After drying the plant materials, the essential oil was obtained by hydro-distillation method. Identification of essential oil constituents was done by using GC and GC/MS. The results showed 29 constituents in this oil that presented 97.3% of total oil. The main components of the oil were P-cymen (25%), E-caryophyllen (14.4%), thymol (9.6%), carvacrol (5.3%) and cumin aldehyde (5.1%). This oil had some similarities and differences with O. vulgare oil with high content of phenols, thymol and carvacrol as well as germacrene D and caryophyllene.
R. Abbas Azimi; F. Sefidkon; Z. Jamzad; Gh. Bakhshi Khaniki
Abstract
Vitex L. (250 species) is a large tropical genus with a few temperate species. Examining the morphological characters of leave, stem, corolla and calyx together, with the micro-morphological observations, revealed the occurrence of four species in the flora of Iran. These species are V. agnus-castus, ...
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Vitex L. (250 species) is a large tropical genus with a few temperate species. Examining the morphological characters of leave, stem, corolla and calyx together, with the micro-morphological observations, revealed the occurrence of four species in the flora of Iran. These species are V. agnus-castus, V. negundo, V. trifolia and V. pseudo-negundo. In this research, four species of Vitex werecollected in flowering stage from their natural habitats. Hydro-distillation method was used to obtaining the essential oils. Essential oils components were identified by capillary GC and GC/MS. In the oil of V. agnus-castus, the main components of essential oil were monoterpenoids (82.8%) with 1,8-cineole (18.5%), α-pinene (17.8%) and limonene (15.7%) as major components. In the oil of V. pseudo-negundo, the main components of the essential oil were sesquiterpenoids (50.7%) which have the highest amount in comparing with other species and include α-guaiene (14.2%), germacrene D (11/6%) and α-cadinol (10%). Also the monoterpenoids of the oil of this species were α-pinene (10.3-24.5%), limonene (4.4-12.8%) and 1,8-cineole (10.9%). In the oil of V. negundo the major components were monoterpenoids (66.6%), among them 1,8-cineole (20.8%) and α-pinene (18.8%) were the main components. In the oil of V. trifolia, the major components were monoterpenoids (67.1%) and the main constituents were identified as sabinene (24.6%) and α- pinene (23.9%). 1,8-cineole was not found in the oil of this species. β-caryophyllene was identified at higher percentage (10.5%) in the oil of V. trifolia
