A. Riazi; N. Majnoun Hosseini; H.A. Naghdi Badi; M.R. Naghavi; Sh. Rezazadeh
Abstract
Hypericin and hyperforin compounds are considered as the main active constituents of Hypericum perforatum L. In this research, phytochemical variations of 25 St. John’s Wort populations in Iran’s natural habitats were investigated. Plants were sampled randomly at the full flowering stage ...
Read More
Hypericin and hyperforin compounds are considered as the main active constituents of Hypericum perforatum L. In this research, phytochemical variations of 25 St. John’s Wort populations in Iran’s natural habitats were investigated. Plants were sampled randomly at the full flowering stage from Golestan, Mazandaran, Gilan, East Azerbaijan, West Azerbaijan, Ardabil, Kurdistan, Hamedan, Kohgiloyeh and Boyr-Ahmad, Qazvin, Zanjan, Khorasan and Tehran provinces during the spring and summer of 2010. Results indicated that the differences in the amounts of hypericin and hyperforin of leaf and flower tissues were found to be significant among populations (P<0.05). Flower tissues had more hypericin and hyperforin amounts as compared to leaf tissues. The amount of hyperforin in both tissues was higher than that of hypericin significantly. Simple correlation analysis showed positive significant correlations between leaf hypericin with dark and light glands density on the leaf and light glands density on the leaf surface area as well as positive significant correlation between leaf hyperforin and leaf hypericin. The principal components analysis indicated that the two components explained 66% of the total variance. The cluster analysis divided these populations into three groups with no consistency in their geographical distribution. In conclusion, the results indicated high variations of phytochemical characters among St. John’s Wort populations in Iran’s natural habitats, which can be utilized in the breeding programs.
M.` Ghorbanpour; N. Majnon Hossieni; Sh. Rezazadeh; M. Omidi; K. Khavazi; M. Hatami; R. Ghafarzadegan
Abstract
In order to indicate the water deficit stress and nitrogen fertilization effects on alkaloids yield and content of Hyoscyamus niger L. plant parts (root and shoot), this experiment was conducted at the full flowering growth stage in greenhouse conditions. Plants were treated with different nitrogen application ...
Read More
In order to indicate the water deficit stress and nitrogen fertilization effects on alkaloids yield and content of Hyoscyamus niger L. plant parts (root and shoot), this experiment was conducted at the full flowering growth stage in greenhouse conditions. Plants were treated with different nitrogen application (0, 75, 150 and 225 kg/ha N as ammonium nitrate in the form of solution, N0-N3) before the commencement of water deficit stress treatment (30, 60 and 90% depletion of water from field capacity, W1-W3). Extracted alkaloids were analyzed by Gas chromatography /mass spectrometery (GC/MS) analysis using a Younglin Acme 6000 GC system equipped with a flame ionization detector (FID) and HP-5MS capillary column (30 m × 0.25 mm, film thickness 0.25 µm). The identification of alkaloids was based on the comparison of their GC retention time and mass spectra data with their standards substances. Results showed that the highest alkaloid content values in root (HYO: 0.281 %DW; SCO: 0.232 % DW) and shoot (HYO: 0.937%DW; SCO: 0.416%DW) were achieved in plants grown under sever water deficit stress (W3) accompanied with nitrogen supply of 225kg/h (N3). The maximum and minimum (20.52 and 8.95mg.plant-1) total alkaloids yield in whole plant were obtained in N2W1 and N3W3 treatments, respectively. The survey results indicated that H. niger in the treatment of moderate water deficit stress (W2) along with 150kg N.h-1 (N2) in addition to having a good amount of content and performance of both alkaloids, it also contained the highest level of SCO, indicating alkaloid quality.
M. Alirezaie Noghondar; H. Arouiee; Sh. Rezazadeh; M. Shoor; R. Taghizad Farid
Abstract
The genus of Colchicum is known for the production of colchicine which some species such as C. kotschyi Boiss. and C. robustum Stefanov are grown in Iran. The level of colchicine varies in different species as well as stages of plant development stages. In the present study, colchicine content and dry ...
Read More
The genus of Colchicum is known for the production of colchicine which some species such as C. kotschyi Boiss. and C. robustum Stefanov are grown in Iran. The level of colchicine varies in different species as well as stages of plant development stages. In the present study, colchicine content and dry matter of corm and seeds in C. kotschyi and C. robustum, growing wild in Iran, were determined during different phenological stages. The plant materials were collected in different seasons (spring, summer, autumn and winter), during 2009-2010 and the amounts of colchicine were determined by high liquid performance chromatography method. The highest amount of colchicine of corm in both species was observed during autumn, in the time close to the beginning of activation of the root, with amount of 0.077 and 0.049% (wt/wt) in C. kotschyi and C. robustum, respectively. The lowest amount of colchicine of corm in C. kotschyi was found to be 0.0058% (wt/wt) during summer, before flowering stage, while the lowest amount of Colchicine in C. robustum was found to be 0.0075% (wt/wt) during winter, synchronous to flowering and vegetative growth. Colchicine content in seeds was found to be 0.128 and 0.0462% (wt/wt) in C. robustum and C. kotschyi, respectively. The highest and the lowest of corm dry matter in both species were found in summer and winter, respectively.
