Phytochemistry (extraction, identification and measurement of active components)
F. Askari; F. Sefidkon; M.A. Soltanipour; M. Mirza; S. Fekri Qomi
Abstract
The genus Pycnocyla belongs to the Apiaceae family and has eight perennial species in Iran. In this study, the essential oil content and chemical compounds of P. nodiflora Decne ex. Boiss. and P. flabellifolia Boiss. were investigated. The aerial parts of plants at seeding stage were collected from two ...
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The genus Pycnocyla belongs to the Apiaceae family and has eight perennial species in Iran. In this study, the essential oil content and chemical compounds of P. nodiflora Decne ex. Boiss. and P. flabellifolia Boiss. were investigated. The aerial parts of plants at seeding stage were collected from two habitats in Hormozgan province through two consecutive years and Kermanshah province in one year, respectively. Stem+leaf [SL] and seed [S] were separated and shade-dried. The essential oil was extracted by Clevenger apparatus and hydrodistillation method for three hours. Percentage and chemical composition of the essential oils were determined by GC and GC/MS. The essential oil content of P. nodiflora was obtained [SL]: 0.23 and 1.21% (two years) and [S]: 0.23% in Tangzagh habitat of Hormozgan and [SL]: 0.02 and 0.06% (two years) and [S]: 0.01% in Abmah habitat. Caryophyllene oxid (7.8-28.1%) and b-eudesmol (15.5-57.2%) were recognized as the main compounds of [SL] and [S] essentials oils of both habitats in this species. In P. flabellifolia, the essential oil content of [SL] and [S] was 0.66 and 0.76%, respectively. The main compounds in [SL] essential oil of this species were cis-β-ocimene (33.8%), trans-β-ocimene (39.6%), and terpinolene (12.9%) and in [S] essential oil were trans-β-ocimene (28%), cis-β-ocimene (31%), and terpinolene (12.6%). According to previous reports on the antimicrobial properties of trans-β-ocimene and cis-β-ocimene compounds on bacteria like Staphylococcus aureus and S. epidermidis and the predominance of these compounds in the essential oil of P. flabellifolia, studies on antimicrobial properties of this species essential oil is recommended.
F. Askari; M. Mirza; M. Golipour; S. Fekri Qomi
Abstract
The genus Achillea has 19 species of herbaceous, perennial and aromatic plant in Iran. This genus of compositae family has complex characteristics. Chamazolene is a major component of essential oil of inflorescence and leaf, reported to be used in food, cosmetic, and pharmaceutical industries. A. millefolium ...
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The genus Achillea has 19 species of herbaceous, perennial and aromatic plant in Iran. This genus of compositae family has complex characteristics. Chamazolene is a major component of essential oil of inflorescence and leaf, reported to be used in food, cosmetic, and pharmaceutical industries. A. millefolium L. subsp. elbursensis is an endemic subspecies of Iran and no studies have been conducted on the cultivation of this species; therefore a preliminary study was conducted on the cultivation and its effect on secondary metabolites. For this purpose, the seeds of Achillea were collected from Dizine area in October 2016. The seeds of Achillea were cultivated in a greenhouse at the beginning of March and seedlings were transplanted to the farm in late April 2017.At flowering stage in August, aerial parts were collected in two consecutive years to obtain the essential oils. To compare the essential oil of cultivated samples with habitat samples, the aerial parts of Achillea were collected from Dizin at the flowering stage in August 2017. The plant parts including leaf and inflorescence were dried in laboratory and were crushed to particles. The essential oils were obtained by hydrodistillation and were analyzed by GC-FID and GC/MS. The color of A. millefolium essential oils was dark blue. The yields of A. millefolium essential oils of leaf and inflorescence (w/w dried weight) from habitat samples were 0.11% and 0.53%, respectively, and from cultivated samples were 0.28% and 0.50% in 2017 and 0.26% and 1.30% in 2018, respectively. Chamazulene was the major constituent of leaf (5.7%) and inflorescence (52.5%) oils in the habitat samples. The content of this compound in cultivated samples was 50.6% and 67.1% in the first year, and 59.6% and 71.3% in the second year, respectively. Another major constituents were caryophyllene alcohol, caryophyllene oxide, camphor, borneol and b-eudesmol. Chamazulene as major compound of the oil, found in all aerial parts of cultivated samples, while, it was found only in the inflorescence of wild sample.
F. Askari; M. Mirza; M. Najafpour Navaei; Sh. Ahmadi
Abstract
Both Centaurea pterocaula Trautv. and Centaurea urvillei DC. are a type of cornflower. The aim of this study is to determine the chemical composition of essential oils of C. urvillei and C. pterocaula for food, cosmetics-health industry and medicinal uses. The plant parts of C. urvillei and C. pterocaula ...
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Both Centaurea pterocaula Trautv. and Centaurea urvillei DC. are a type of cornflower. The aim of this study is to determine the chemical composition of essential oils of C. urvillei and C. pterocaula for food, cosmetics-health industry and medicinal uses. The plant parts of C. urvillei and C. pterocaula were collected at flowering stage from Urmia (Ghasemloo Valley) and Zanjan in 2010 and 2011. The plant parts including stem, leaf and inflorescence were dried in laboratory and crushed to particles. The essential oils were obtained by hydrodistillation and were analyzed by GC-FID and GC/MS. The essential oil yield of stem plus leaf and inflorescence of Centaurea pterocaula (w/w dried weight) were 0.06% and 0.09%, respectively. The major constituents of stem plus leaf and inflorescence essential oils were germacrene D (20.4% and 5.4%), thymol (10.6% and 3.8%), cyperene (3.5% and 11.5%), spathulenol (7.7% and 4.9%), caryophyllene oxide (6.8% and 13.4%) and E-caryophyllene (4.9% and 8.1%), respectively. The essential oil yield of stem plus leaf and inflorescence of Centaurea urvillei (w/w dried weight) were 0.023% and 0.065%, respectively. The major constituents of stem plus leaf oils were benzyl benzoate (38.7%), caryophyllene oxide (17.9%) and b-eudesmol (19.8%) and the major constituents of inflorescence oil were caryophyllene oxide (25.7%), spathulenol (14.8%) and eudesma-4(15)-7-dien-1-β-ol (21.0%).
F. Askari; Sh. Ahmadi
Abstract
Pimpinella,with about 170-180 species in the world, is one of the largest genus of Apiaceae. Based on the flora of Iran, there are 25 species of Pimpinella in Iranian plateau, so that 22 species with six endemic species are found in Iran. P. olivierioides Boiss. & Hausskn. is distributed in West ...
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Pimpinella,with about 170-180 species in the world, is one of the largest genus of Apiaceae. Based on the flora of Iran, there are 25 species of Pimpinella in Iranian plateau, so that 22 species with six endemic species are found in Iran. P. olivierioides Boiss. & Hausskn. is distributed in West and Central Iran. The aim of this study was to determine chemical composition of P. olivierioides essential oil. The plant parts of P. olivieroides were collected at flowering stage from Nahavand (Hamedan Province), on July 2012. The plant organs including stem and leaf and inflorescence were dried in laboratory and crushed to particles. The essential oils were obtained by hydrodistillation and analyzed by GC and GC/MS. The yield of stem and leaf essential oils (w/w dried weight) was 0.09%, root 0.52% and inflorescence 0.57%. The major components of stem and leaf, root and inflorescence essential oils were Germacrene D (36.5%, 21.9% and 11.1%), Bicyclogermacrene (7.5%, 15.9% and 4.4%), respectively. b-bisabolene (24.9%) was found only in root essential oil. Trans-pinocamphene (14.7%) and cis-pinocamphene (13.4%) were found only in stem and leaf essential oils. The interesting thing about the essential oils was their colors. The color of stem and leaf, root and inflorescence essential oils was light blue, blue, and green grass, respectively. Our results clearly showed that very different compositions were found in the essential oil of P. olivierioides organs.
F. Askari; E. Sharifi Ashorabadi; M. Mirza; M. Teimouri; E. Ehsani
Abstract
This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized ...
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This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized design with four replications. In this experiment, collection locality at six levels including the habitats of West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan, essential oil dilution at three levels including one fifth (1:5), one twenty-fifth (1:25), one fiftieth (1:50) and comparison with the antibiotics of ciprofloxacin and ceftizoxime, and studied microorganisms at five levels including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Candida albicans were investigated. The seeds were collected from different habitats and cultivated in the field of Research Institute of Forests and Rangelands. The essential oil was isolated from shoots by hydro-distillation. Chemical compositions of the oils were analyzed by GC and GC/MS. The essential oil yield obtained from different localities varied between 0.39% to 0.83% (w/w). The major constituents of the essential oil obtained from the samples collected from West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan were as follows: West Azarbaijan: E-caryophyllene (26.0%) and camphor (24.2%); Tehran: geranial (30.9%) and geranyl acetate (23.9%); Zanjan: linalool (23.5%) and 1,8-cineol (22.2%); Qazvin: thymol (30.3%) and carvacrol (30.1%); Kurdistan: linalool (17.2%) and geranyl acetate (12.7%), and Guilan: α–terpineol (31.2%) and geraniol (11.2%). Analysis of variance of inhibition zone diameter of T. pubescence essential oil showed significant difference (p<0.05) among collection locality, microorganism type, essential oil dilution and their interaction. Mean comparisons of the collection locality, microorganism type and essential oil dilution showed that the highest inhibition zone diameter (35.5mm) was recorded for the essential oil obtained from West Azarbaijan, ciprofloxacin against Bacillus subtilis. The lowest inhibition zone diameter (8.0 mm) was observed in the essential oil obtained from West Azerbaijan with essential oil dilution of 1:50 against P. aeruginosa. According to the MIC and MBC results, the most and the least antimicrobial activity was recorded for the essential oils obtained from the seeds collected from Tehran and Kurdistan, respectively.
T. Mozafari Dehshiri; F. Sefidkon; F. Asgari; Gh. Bakhshi Khaniki
Abstract
Pimpinella aurea DC. is one of the most abundant perennial species from Pimpinella genus in Iran, distributed in northwest, west, center, northeast, and southeast of Iran. This aromatic species contains essential oil with antimicrobial effect. In this research, to study the quality and quantity of seed ...
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Pimpinella aurea DC. is one of the most abundant perennial species from Pimpinella genus in Iran, distributed in northwest, west, center, northeast, and southeast of Iran. This aromatic species contains essential oil with antimicrobial effect. In this research, to study the quality and quantity of seed essential oil of Pimpinella aurea and also the effect of seed size on essential oil content and composition of Pimpinella aurea, seeds were collected from three localities in Tehran province (Tochal, Vardavard and Lavasanat) and were, after drying, separated by sieves having mesh sizes of 20, 25 and 30. All samples were subjected to hydro-distillation, individually, for obtaining their essential oils. The mean oil yields for Tochal seeds were calculated to be 4.01%, 3.20% and 1.12% for 20, 25 and 30 meshes, respectively. These values were 5.69%, 3.20% and 1.94% for the samples from Vardavard and 3.21%, 2.70% and 1.85% for Lavasanat samples. Results showed that the oil yield decreased remarkably with decreasing the size of the seeds. The essential oils were analyzed by GC and GC/MS for the identification of their chemical composition. β-bisabolene was identified as the main component in the oils collected from different habitats (57.2-83.6%). The other major component was epoxy allo-aromadendrene (3.9-30.6%). According to the obtained results, seed size had no considerable effect on essential oil composition. In addition, more oil yield and β-bisabolene content were recorded for the seeds collected from Vardavard (with a warmer and dryer habitat).
F. Askari; V. Mozaffarian; E. Parsa
Abstract
Centaurea zuvandica(Sosn.) Sosn.is a perennial species, distributed in North and North-West of Iran. The shoots of C. zuvandica were collected at flowering stage from Firoozkoh, Ghaemshahr and Chaloos Road, in late June 2011. The plant parts were dried at ambient temperature and milled to small ...
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Centaurea zuvandica(Sosn.) Sosn.is a perennial species, distributed in North and North-West of Iran. The shoots of C. zuvandica were collected at flowering stage from Firoozkoh, Ghaemshahr and Chaloos Road, in late June 2011. The plant parts were dried at ambient temperature and milled to small particles. The essential oils of stem as well as leaves and inflorescences were obtained by hydrodistillation and were analyzed by GC and GC/MS. Essential oil yield of different organs varied (w/w dried weight) between 0.02% to 0.07%. The major constituents were: spathulenol (>28.8%), thymol (>21.7%), caryophyllene oxide (>20.9%), linalool (>19.5%), germacrene D (>16.3%) and carvacrol (>6.8%). According to the obtained results, a high variability was found for the major essential oil constituents among three studied localities and even among different organs of a locality. The major constituent in the stem and leaf oils, collected from Chaloos Road and Firoozkoh localities, was thymol (17.8% and 21.7%). Linalool (19.5% and 9.7%) was the major constituent in inflorescence oils, collected from Chaloos Road and Firoozkoh localities. For the samples collected from Ghaemshahr, caryophyllene oxide (20.9%) was identified as the major constituent in stem and leaf oil.
F. Askari; M. Mirza
Abstract
Centaurea depressa M. Bieb. (wheat flower) is a medicinal plant that its extraction is used for eye irrigation and somewhat reduces gastrointestinal irritation. This research was aimed to investigate the essential oil of C. depressa. Different organs of C. depressa were collected from Damavand and Tehran ...
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Centaurea depressa M. Bieb. (wheat flower) is a medicinal plant that its extraction is used for eye irrigation and somewhat reduces gastrointestinal irritation. This research was aimed to investigate the essential oil of C. depressa. Different organs of C. depressa were collected from Damavand and Tehran at flowering stage. The plant organs including stem plus leaf, root and inflorescence were separated and dried at laboratory temperature and then were ground to small particles. Essential oils were obtained by hydro-distillation method and analyzed by GC and GC/MS. The yield of essential oil of stem plus leaf, root and inflorescence of Damavand samples were 0.18%, 0.13% and 0.07% w/w (dried weight), respectively and for Tehran samples the values of 0.10%, 0.13% and 0.08% were recorded, respectively. In Damavand, the major constituents of essential oil obtained from stem plus leaf, root and inflorescence were thymol (56.5%), spathulenol (12.7%) and germacrene D (9.9%); pentadecadiene-1-ol (32.2%) and Z-7-hexadecene (29.5%); and germacrene D (32.4%), germacrene B (9.4%) and thymol (8.7%), respectively. In Tehran, the major constituents of essential oil obtained from stem plus leaf, root and inflorescence were Spathulenol (25.4%), Eudesms-4(15)-7-diene-1-b-ol (14.5%) and germacrene D (13.7%); tetradecanal (30.7%), caryophyllene oxide (23.7%) and Z-7-hexadecene (22.3%); and tetradecanal (82.3%) and Z-7-hexadecene (15.5%), respectively. According to the results, it can be concluded that there is variability in essential oil compositions of the two sites and even in different plant's organs.
F. Askari; E. Sharifi Ashorabadi
Abstract
The current study was performed to evaluate the effect of planting date and locality of seed on seed yield of Pimpinella affinis Ledeb. The experiment design was factorial done using a randomized complete blocks design with three replications. Treatments included locations from where seeds were collected ...
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The current study was performed to evaluate the effect of planting date and locality of seed on seed yield of Pimpinella affinis Ledeb. The experiment design was factorial done using a randomized complete blocks design with three replications. Treatments included locations from where seeds were collected (Khojir, Noshahr and Chalous Road areas) and planting dates (November 6th, December 6th and April 9th in 2006 and 2007). The experiment was performed in Alborz farm research complex located in Karaj. The results of variance analysis of the experiment in 2006 showed that the effect of planting date on seed yield was not significant while the effect of locality and interaction effect between planting date and locality was statistically significant at 1% significance level. Means comparison of treatments showed no significant differences between the yield seed of planting dates of November 6th and December 6th, while December cultivation's yield seed was higher (64.467 Kg/ha). The effect of locality on yield seed was significant as yield seed of Khojir locality (175.1 Kg/ha) was more than that of Chalous and Noshahr localities. Interaction effects between locality and planting date on yield seed were statistically significant at 5% significance level. Yield seed of December cultivation from Khojir locality (191.4 Kg/ha) was the highest amount. The results of variation analysis in 2007 showed that the effects of planting date, locality and their interaction on seed yield were significant at 1% significance level. Means comparison of treatments showed no significant differences between the yield seed of planting dates of November 6th and December 6th, but they had significant difference with April cultivation, while November cultivation's yield seed was higher (218.9 Kg/ha). The effect of locality on yield seed was significant as yield seed of Khojir locality (281.9 Kg/ha) was more than that of Noshahr locality. Interaction effects between locality and planting date on yield seed were significant as yield seed of November and December cultivations from Khojir locality (404.6 and 397.9 Kg/ha) was the highest amount. According to the results of cultivation for two years it can be concluded that autumn cultivation had more yield seed and biological function than that of spring cultivation due to having long growth period. In addition, seeds collected from Khojir locality had the highest yield seed and biological function among three studied localities.
F. Askari; Sh. Ahmadi; S. Meshkizadeh; M. Naderi Haji Bagher Kandi; A. Bahmanzadegan Jahromi
Abstract
Pimpinella deverroides (Boiss.) Boiss. is one of the nine endemic species of Pimpinella in Iran. It is distributed in Iran and Touran Regions. The plantwas collected at two consecutive years (2005 and 2006) from Lorestan province in flowering and seed stages. Essential oils were isolated by hydro-distillation ...
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Pimpinella deverroides (Boiss.) Boiss. is one of the nine endemic species of Pimpinella in Iran. It is distributed in Iran and Touran Regions. The plantwas collected at two consecutive years (2005 and 2006) from Lorestan province in flowering and seed stages. Essential oils were isolated by hydro-distillation from the stems and leaves, inflorescence and seed separately and were analyzed by GC and GC/MS. The yields of the stems and leaves, inflorescence and seed oils of P. deveroides, were 0.6%, 2.5% and 7.1% w/w, respectively in 2005 and 1.3%, 2.4% and 7.5% w/w in 2006. The yields of seed oils were higher than the yields of stems plus the leaves and inflorescence oils. Pregeijerene (35.5% to 67%) and Geijerene (8.2% to 14.1%) were the major constituents in all oils. According to this study P. deveroides is important for the highest quantity oil in seed.
Sh. Ahmadi; F. Sefidkon; P. Babakhanlo; F. Asgari; K. Khademi; N. Valizadeh; M.A. Karimifar
Abstract
The genus of Satureja consist of 30 species in world. Satureja bachtiarica Bunge is one of the endemic species of this genus in Iran. In order to identify the quality changes of essential oil of Satureja bachtiarica, we planted the specimen in 1382 in Khorram Abad on the 36 plot, using the split factorial ...
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The genus of Satureja consist of 30 species in world. Satureja bachtiarica Bunge is one of the endemic species of this genus in Iran. In order to identify the quality changes of essential oil of Satureja bachtiarica, we planted the specimen in 1382 in Khorram Abad on the 36 plot, using the split factorial design in randomized complete block with 3 replications. Aerial parts of plants were collected in two stages (before and full flowering) from field and provenance in the second year. The plant materials were dried and hydro-distilled in order to obtain their essential oils. The oils were analyzed by capillary GC and GC/MS. The main component of essential oil before flowering stage were carvacrol (20٪), P-cymene (36.5٪) and thymol (19.2.(٪ In the flowering stage 21 compounds were characterized in the oil with carvacrol (25.5٪), p-cymene (23.2٪), thymol (0.5(٪ and mentone (18.5 (٪as the main constituents. Fifteen compounds were characterized in the oil of field sample (Khorram Abad). The main constituents of the oil were carvacrol (48.6٪) and p-cymene (28.6٪) before flowering stage. In the flowering stage 13 compounds were characterized in the oil with carvacrol (62.3٪) and p-cymene (21.2٪) as the main constituents. The oil yields of cultivated and wild S. bachtiarica samples were 1.1, 2.08% and 1.8, 1.1% before and full flowering stages, respectively. The amount of carvacrol in Satureja bachtiarica oils were 48.6%, 62.3% and 20%, 25.8%before and full flowering stages, in field and provenance, respectively. These results showed that variation of ecological characters like altitude, temperature, humidity and climate had effect on percentage of carvacrol.
F. Sefidkon; L. Sadeghzadeh; M. Teimouri; F. Asgari; Sh. Ahmadi
Volume 23, Issue 2 , August 2007, , Pages 174-182
Abstract
The genus Satureja represents 15 species in Iran, 9 of them are endemic. In this study, the aerial parts of Satureja khuzistanica Jamzad and Satureja bachtiarica Bunge were collected at two stage of plant growth (before flowering and full flowering) from their natural habitats. After drying the plant ...
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The genus Satureja represents 15 species in Iran, 9 of them are endemic. In this study, the aerial parts of Satureja khuzistanica Jamzad and Satureja bachtiarica Bunge were collected at two stage of plant growth (before flowering and full flowering) from their natural habitats. After drying the plant materials in shade, essential oils were obtained by hydro-distillation and analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. The results showed the oil of S. bachtiarica contained 20% carvacrol and 19% thymol before flowering and 26% carvacrol and 5% thymol at full flowering stage, as main components. The oil of S. khuzistanica, in both harvesting time contained about 90% carvacrol. Due to the antimicrobial effect of phenolic compounds, thymol and carvacrol, the antimicrobial effects of these oils were determined against five gram positive bacteria (Bacillus subtilis, Bacillus cereus, Micrococcus loteus, Staphylococcussp. and Staphylococcus areous) and three gram negative bacteria (Kellebsiella pneumonia, Kellebsiella oxytoca and Pseudomonas aeroginosa). The result showed the oil of S. khuzistanica had strong anti-bacterial effect in both harvesting stage. The anti-bacterial effect of S. bachtiarica oil was stronger before flowering stage, because of more percentage of phenolic compounds. So these oils can be used instead of synthetic antibiotics that their resistance against bacteria increased daily.
F. Askari; F. Sefidkon; Sh. Ahmadi
Volume 22, Issue 2 , September 2006, , Pages 98-104
Abstract
trans-a-Bergamotene is an aromatic compound that is used for synthesize of the other aromatic compounds. Pimpinella affinis Ledeb. grows wild in different regions of Iran, north, north-west, center, eastern and north-east. In This research, the aerial parts and seeds of P. affinis werecollected ...
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trans-a-Bergamotene is an aromatic compound that is used for synthesize of the other aromatic compounds. Pimpinella affinis Ledeb. grows wild in different regions of Iran, north, north-west, center, eastern and north-east. In This research, the aerial parts and seeds of P. affinis werecollected from Noshahr in 2003 and 2004. Essential oils were isolated by hydro-distillation. The yields of stem plus the leaf, inflorescence and seed oils for the first year were 0.26%, 1/1% and 4.1% w/w and for the second year were 0.26%, 0.86% and 2.45%, respectively. Four constituents in stem plus the leaf oil, five constituents in inflorescence oil and six constituents in seed oil were identified in samples of 2003. Six constituents in stem plus the leaf oil, fifteen constituents in inflorescence oil and six constituents in seed oil were identified in samples of 2004. Major constituent of the stem plus the leaf, inflorescence, and seed oils was trans-a-Bergamotene (91.1%, 96.2% and 90.2% in 2003 and 94.3% 84.9% and 95.5% in 2004, respectively).
F. Askari; F. Sefidkon; S. Meshkizadeh
Abstract
Essential oils were isolated by hydro-distillation from the arial parts and seeds of Pimpinella eriocarpa Banks & Soland Individually, that were collected from khojir (Northeast of Tehran province). The yields of arial parts and seed oils were 1.3% and 5.7%w/w, respectively. Fifteen constituents ...
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Essential oils were isolated by hydro-distillation from the arial parts and seeds of Pimpinella eriocarpa Banks & Soland Individually, that were collected from khojir (Northeast of Tehran province). The yields of arial parts and seed oils were 1.3% and 5.7%w/w, respectively. Fifteen constituents in the aerial parts oil and eight constituents in seed oil were identified. Major constituents of the aerial parts oil were Pregeijerene (59.9%), Limonene (17.6%) and Elemicin (12.5%). Major constituents of the seed oil were Limonene (49.3%) and Elemicin (44.5%). Two major compounds (Limonene and Elemicin) were common in both of the oils. Seven trace constituents of the aerial parts oil were not found in the seed oil at all.
F. Askari; F. Sefidkon
Volume 20, Issue 2 , July 2004, , Pages 229-237
Abstract
Melissa officinalis L. is a very easily grown plant that habitats in south and Eastern of Europe, Spain and Asia. The plant of Melissa collected from three fields in Fars, Tehran and Semnan provinces. Essential oils were isolated by hydro-distillation. The yields of Essential oils of Fars sample (Shiraz), ...
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Melissa officinalis L. is a very easily grown plant that habitats in south and Eastern of Europe, Spain and Asia. The plant of Melissa collected from three fields in Fars, Tehran and Semnan provinces. Essential oils were isolated by hydro-distillation. The yields of Essential oils of Fars sample (Shiraz), Tehran sample (Karaj) and Semnan sample were 0.14%, 0.25% and 0.26% (w/w) respectively. The oils were analyzed by GC (9A-Shimadzu) and GC/MS (Varian 3400). Seventeen constituents at Fars sample oil, seventeen constituents at Tehran sample oil and fourteen constituents at Semnan sample oil were identified respectively. Major constituents of Fars sample were citronellal (25.4%), b-caryophyllene (11.3%), thymol (10.5%), globolol (6.1%), caryophyllene oxide (6.5%) and geraniol (5.7%). Major constituents of Karaj sample oil were citronellal (9.3%), carvacrol (31.8 %), Humulene epoxide (25.4 %) and a-humulene (10.2%). Major constituents of Semnan samples oil were citronellal (43.8%), b-caryophyllene (13.5%), thymol (7.9%), globolol (6.8%), neral (5.3%) and geranial (5.0%). Fourteen constituents were common at all sample oil. Major constituent at all samples oil was citronellal. Four major constituents were common at Fars and Semnan samples oil.
F. Askari; F. Sefidkon; M. Mirza; S. Meshkizadeh
Volume 19, Issue 3 , October 2003, , Pages 239-254
Abstract
The genus of Pimpinella presents about 20 species, which are found wild in different regions of Iran. Most of them are annual. Two of the more distributed and prenial of these species is P. eurea and P. tragium. Essential oils were isolated by hydro-distillation from the seeds, flowers, and stems of ...
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The genus of Pimpinella presents about 20 species, which are found wild in different regions of Iran. Most of them are annual. Two of the more distributed and prenial of these species is P. eurea and P. tragium. Essential oils were isolated by hydro-distillation from the seeds, flowers, and stems of Pimpinella aurea DC.Individually, that were collected from Fasham (Northwest of Tehran province) and Tochal (North of Tehran province). The yields of seeds, flowers, and stems oils of Fasham sample were 1.97%, 1.54% and 0.44% w/w, and the yields of seeds, flowers, and stems oils of Tochal samples were 1.2%, 0.47% and 0.48% w/w, respectively. The oils were analyzed by GC (9A-Shimadzu) and GC/MS (Varian 3400). Eight and four constituents at seed oil, thirty-two and thirty-four constituents at flower oil and eighteen and twenty constituents at stem oil of Fasham and Tochal samples were identified respectively. In the Fasham samples: Major constituents of the stem plus the leaf oil were: 1,8-Cineol and Limonene (21.4%), Viridiflorol (12.8%), a-Pinene (11.5%) and Kessane (10.5%). Major constituents of the flower oil were: Viridiflorol (32.5%) and b-Bisabolene (29.5%). Major constituents of the seed oil were: b-Bisabolene (50.8%) and Viridiflorol (37.0%). In the Tochal samples: Major constituents of the stem plus the leaf oil were: b-Bisabolene (18.3%), Geranyl acetate (14.7%), Geranyl2-methyl butyrate (9.0%) and Limonene (7.3%). Major constituents of the flower oil were: b-Bisabolene (55.2%) and a-zingiberene (8.7%). Major constituents of the seed oil were: b-Bisabolene (76.5%) and Caryophyllene oxide (21.4%).
F. Askari; F. Sefidkon; M. Mirza
Volume 19, Issue 2 , August 2003, , Pages 125-136
Abstract
One of the more distributed of The genus of Thymus is T. pubescens that is widespread in Tehran provinces up to 1800 m height and often widespread on flat and rocky surface. The aerial parts of Thymus pubescens were collected at four various localities in the Tehran province: Lar valley and Damavand ...
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One of the more distributed of The genus of Thymus is T. pubescens that is widespread in Tehran provinces up to 1800 m height and often widespread on flat and rocky surface. The aerial parts of Thymus pubescens were collected at four various localities in the Tehran province: Lar valley and Damavand (East of Tehran province), Sirachal and Fasham (Northwest of Tehran province). Essential oils were isolated by steam distillation from the plant material at two stages, before flowering (BF) and at full flowering (FF). The yields arranged between 0.34% and 0.86% at BF stage and between 0.40% and 2.03% at FF stage. In most sites of collection the oil content at BF stage was less than FF stage and the oil percentage of plant material from Lar valley and Damavand was more than of Sirachal and Fasham. At BF stage 25 compounds and at FF stage 26 compounds were characterized by means of GC and GC/MS. Major constituents were: carvacrol, thymol, g-terpinene, r-cymene, borneol, methy carvacrol, b-caryophyllene, 1,8-cineol + limonene and Geraniol. Twenty-three constituents were common at two stages. Geranyl acetate and thymyl acetate were found just at BF stage and geranial, nerol, and thymodihydroquinone were found just at FF stage.
