M. Dadashpour; I Rasooli; F. sefidkon; E. Zaad Hosseingholi; Sh. Darvish Alipour Astaneh
Abstract
In the present study, the antimicrobial properties of Satureja sahendica Bornm. and summer savory (Satureja hortensis L.) essential oils were studied. The bacterial strains sensitive to the oils were in Candida albicans> E. coli> S. aureus> P. aeruginosa order. Minimum inhibitory and bactericidal ...
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In the present study, the antimicrobial properties of Satureja sahendica Bornm. and summer savory (Satureja hortensis L.) essential oils were studied. The bacterial strains sensitive to the oils were in Candida albicans> E. coli> S. aureus> P. aeruginosa order. Minimum inhibitory and bactericidal concentrations were observed in the range of 0.5-10mg/ml. Total phenolics of S. sahendica and the S. hortensis were 170.5±8.53 and 47.25±2.14 µg Gallic acid equivalent per mg sample. Antioxidative property of the oils was carried out using DPPH free radical scavenging and beta-carotene bleaching tests and the results were compared with the standard synthetic antioxidants. Lipid peroxidation inhibitions were comparable to or higher than the synthetic antioxidants. The concentrations from S. sahendica and S. hortensis oils required for 50% free radical scavenging (IC50) were 6.25 and 5.82 μg/ml respectively. Ferric-reducing antioxidant power (FRAP) of S. sahendica oil was greater than that of the S. hortensis oil. The superoxide anion radical scavenging activities of S. sahendica and S. hortensis oils were 55.28%±1.16 and 21.81%±2.46 at 15µg/ml oil with an IC50 of 9.4 and 101.2 µg respectively. Tyrosinase activity of one µg S. sahendica oil was 47.88±%1.33 while that of the S. hortensis oil at 15 µg level was 15.35%±2.9. In conclusion, the results from biological properties of Satureja sahendica are indicative of its potentials for food and drug industries applications.
A. Kheiry; F. Sefidkon; M. Delshad; M.R. Fattahi Moghaddam; A. Izadi
Abstract
Achillea millefolium L. from Asteraceae family is considered as an important medicinal plant in the world and also in our country which has considerable secondary metabolites, especially essential oils. Active substances of this plant are used not only in old and modern medicine but also in hygienic, ...
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Achillea millefolium L. from Asteraceae family is considered as an important medicinal plant in the world and also in our country which has considerable secondary metabolites, especially essential oils. Active substances of this plant are used not only in old and modern medicine but also in hygienic, cosmetic and medical industry. In this research, we studied yield and chemical composition of yarrow essential oils from 15 different ecological regions of Iran. For essential oils isolation, the aerial parts of the plant were collected from habitats, in June and July 2009 at full flowering stage. Samples were dried at room temperature and the essential oils were prepared by hydrodistillation method.The oil yields were calculated on dry weight basis and the analysis of the essential oils was carried out by GC and GC-MS. The results showed that total content of essential oils was variable from 0.32 - 0.72% in different populations. The color of the essential oils varied from pale yellow to dark blue and a close relation was found between composition and color of the essential oils. According to the results, essential oil of Achillea millefolium L. in populations of Iran showed a great diversity with regard to the type of the chemical compositions (high chemical polymorphism). Regarding the major components of the oils, seven chemotypes were identified as follows: 1) 1,8-cineole, 2) p-Cymene, 3) cubenol, 4) chamazulene, 5) chrysanthenone + camphor, 6) Lavandulyl acetate + camphor, and 7) camphor + Limonene.
M. Hamisi; F. Sefidkon; M. Nasri; M.H. Lebaschi
Abstract
For investigating the effects of nitrogen, phosphor and bovine fertilizers on essential oil content and composition of feverfew (Tanacetum parthenium L.), an experiment was arranged based on Complete Randomized Blocks Design, in three replication, from February 2009 to October 2010, at research farm ...
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For investigating the effects of nitrogen, phosphor and bovine fertilizers on essential oil content and composition of feverfew (Tanacetum parthenium L.), an experiment was arranged based on Complete Randomized Blocks Design, in three replication, from February 2009 to October 2010, at research farm of Research Institute of Forests and Rangelands. The treatments were: 1- nitrogen fertilizer in three levels (60, 90, 120 kg/ha), 2- triple superphosphate fertilizer in two levels (60 and 80 kg/ha) and the 3- bovine fertilizer in three levels (0, 15 and 30 tons/ha). The aerial parts of the plants were collected at the beginning of flowering and after drying their essential oils were obtained by hydro-distillation and analyzed by GC and GC/MS. The results showed that different levels of urea fertilizer had significant effects on essential oil percentage and yield at level of 1%. Also, nitrogen fertilizer had significant effects on percentage of chrysanthenyl acetate and santolina triene. Bovine fertilizer had also significant effects on oil percentage and yield, camphor, camphene and santolina triene percentage at level of 5%. The highest amounts of oil percentage (0.32%) and yield (19 kg/h) were obtained by application of 60 Kg/h urea fertilizer. In addition, GC and GC/MS analysis showed different levels of bovine fertilizer had significant effect on percentages of main components of feverfew essential oil.
S. Shahverdi; F. Sefidkon; Z. Jamzad; T. Nejhadsattari
Abstract
Origanum strobilaceum Mobayen & Ghahreman belongs to Lamiaceae. Thirty eight species of the genus were known so far which are categorized in 9 sections. The species is aromatic and its essential oil compositions are valuable. In this research, for the first time, the essential oil content and composition ...
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Origanum strobilaceum Mobayen & Ghahreman belongs to Lamiaceae. Thirty eight species of the genus were known so far which are categorized in 9 sections. The species is aromatic and its essential oil compositions are valuable. In this research, for the first time, the essential oil content and composition of O. strobilaceum was studied. The aerial parts of the plant were collected from janat rodbar in Mazandaran Province road at full flowering stage. After drying the plant materials, the essential oil was obtained by hydro-distillation method. Identification of essential oil constituents was done by using GC and GC/MS. The results showed 29 constituents in this oil that presented 97.3% of total oil. The main components of the oil were P-cymen (25%), E-caryophyllen (14.4%), thymol (9.6%), carvacrol (5.3%) and cumin aldehyde (5.1%). This oil had some similarities and differences with O. vulgare oil with high content of phenols, thymol and carvacrol as well as germacrene D and caryophyllene.
M. Habibzadeh; F. Sefidkon; Sh. Fatemi
Abstract
Limonene, trans-α-bergamoten, geijeren, elemicine and germacrene B are being used widely in the formulation of pharmaceutical products, cosmetics, hygienic applications, and aromatization. These compounds are found in essential oils of Pimpinella affinis Ledeb. (a species native to Iran and Turkey). ...
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Limonene, trans-α-bergamoten, geijeren, elemicine and germacrene B are being used widely in the formulation of pharmaceutical products, cosmetics, hygienic applications, and aromatization. These compounds are found in essential oils of Pimpinella affinis Ledeb. (a species native to Iran and Turkey). In the present study, aerial parts of the plant were collected in flowering and maturity stages from Alborz Research Station of Karaj, and dried in room temperature. Three methods of oil extraction including hydro distillation, water & steam distillation and direct steam distillation were applied based upon Taguchi statistical method in three levels to determine the quantitative and qualitative effects of the parameters namely method, time and mesh on essential oil. Essential oil yields were calculated based on dry weight. Identification of the compounds and determination of compounds percentage were done by GC and GC/MS. Mean comparisons of essential oil yield showed that more content of limonene, trans-α-bergamoten and geijeren was obtained by mesh size 20 in hydro-distillation and steam distillation methods. Mesh size 40 and time 2.5 h gave the best result for elemicine and germacren B in steam distillation method. The maximum and minimum essential oil yield was respectively obtained by mesh size 20 and 14 in water & steam distillation method. Therefore, deseirable percentage and compounds of the essential oil could be achieved from Pimpinella affinis Ledeb. by selection of the condition of essential oil extraction.
E. Jamshidi; A. Ghalavand; F. Sefidkon; E. Goltaph
Abstract
In order to study the effects of different nutrition systems (Organic and Chemical) on quantitative and qualitative characteristics of Fennel (Foeniculum valgare Mill.) under water deficit stress, an experiment was conducted as split plot in a randomized complete block design with three replications ...
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In order to study the effects of different nutrition systems (Organic and Chemical) on quantitative and qualitative characteristics of Fennel (Foeniculum valgare Mill.) under water deficit stress, an experiment was conducted as split plot in a randomized complete block design with three replications at the field station of Tarbiat Modares University in Peykan Shahr during cropping season of 2009. In this study, the main plots were three irrigation regime levels (Soil water content of 40% as the control treatment (C1), 60% (C2) and 80% (C3)) and sub-plots were different nutrition systems (10 ton/ha cattle manure, 10 ton/ha spent mushroom compost, 5 ton/ha cattle manure + 5 tan/ha spent mushroom compost and chemical fertilizer). Although the result showed that water deficit stress caused an increase in essential oil percentage, grain yield and essential oil yield decreased extremely under water deficit stress. The results showed that different nutrition systems significantly affected grain yield, essential oil yield and fenchone (p ≤ 0.01). Mean comparisons showed that using mushroom compost in favorable irrigation condition resulted in maximum essential oil yield (31.8 Kg.ha-1). This result was confirmed as the higher grain yield (1289.8 Kg.ha-1) obtained in the same treatment.
S.F. Mirahmadi; M.R. Hasandokht; F. Sefidkon; M.E. Hassani
Abstract
Achillea biebersteinii Afan. is a member of Asteraceae and occurs wild in different parts of the world including Europe, Turkey, Iran and central Asia. In addition to traditional medicine, the plant is also considered in modern medicine and different industries because of its essential oil characteristics. ...
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Achillea biebersteinii Afan. is a member of Asteraceae and occurs wild in different parts of the world including Europe, Turkey, Iran and central Asia. In addition to traditional medicine, the plant is also considered in modern medicine and different industries because of its essential oil characteristics. The present study was conducted to investigate the essential oil content and composition of nine populations of A. biebersteinii collected from different ports of Khorassan Province. For this purpose, after calculating essential oil content of each population with three independent experiments, chemical constituents of oils were identified by means of GC and GC-MS analyses. The results showed that Golool (1.62%) and Chelmir (1.60%) contained the highest amount of essential oil. Totally, 23 components were characterized in the essential oil of all populations with oxygenated monoterpens (47.9-73.1%) as the principle fraction. While 1,8-cineole, nepetalactone, p-cymene, a-terpinene and cis-chrysanthenyl acetate were identified as the prevalent constituents of all investigated essential oils, lavandulyl 2-methyl butyrate and cis-chrysanthenol were the rarest ones. Due to the high content of essential oil and high levels of 1,8-cineole, nepetalactone derivatives and p-cymene which are well-known biologically active compounds, Golool and Azghad populations seem to be suitable for application in relevant industries.
A.R. Yavari; V. Nazeri; F. Sefidkon; Z. Zamani; M.E. Hassani
Abstract
In the present study, the genetic variation within and among some populations of Thymus migricus Klokov & Desj.-Shost was investigated using the RAPD markers. Twenty-one decamer RAPD primers produced 310 unique bands. RAPD analysis showed 14 monomorphic and 296 polymorphic bands in different genotypes. ...
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In the present study, the genetic variation within and among some populations of Thymus migricus Klokov & Desj.-Shost was investigated using the RAPD markers. Twenty-one decamer RAPD primers produced 310 unique bands. RAPD analysis showed 14 monomorphic and 296 polymorphic bands in different genotypes. The number of polymorphic bands per primer varied from 5 to 20 with a mean of 14.09. Genetic distance was measured by Nei’s coefficient and cluster analysis was carried out. A dendrogram was drawn based on genetic distance data, applying the UPGMA clustering method. Analysis of molecular variance (AMOVA) was performed. According to the dendrogram, among T. migricus populations, Band and Jolfa populations had maximum differences with a distance of 0.130. Evaluation of genetic diversity within populations with an average of Nei’s gene diversity analysis and Shannon’s information index, showed that diversity within population of Jolfa (h = 0.196 & I = 0.294) was more than other populations while genetic diversity within population of Ghushchi (h = 0.139 & I = 0.209) was less than other populations. Mean of Fst and Nm indexes which show gene flow among populations, were 0.30 and 1.14, indicating a greater gene flow among five populations of T. migricus. The results of the present study showed that there was a greater level of genetic variation in the Iranian natural populations of T. migricus which could be applied for future breeding programs.
Sh. Khalesro; A. Ghalavand; F. Sefidkon; A. Asgharzadeh
Abstract
Anise (Pimpinella anisum L.) is one of the aromatic and medicinal plants. Anethol is the most important component of its essential oil used in medicinal and food industries. The aim of this experiment was to determine the effect of vermicompost levels (0, 5 and 10 t.ha-1), mixture of Azotobacter, Azospirillum ...
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Anise (Pimpinella anisum L.) is one of the aromatic and medicinal plants. Anethol is the most important component of its essential oil used in medicinal and food industries. The aim of this experiment was to determine the effect of vermicompost levels (0, 5 and 10 t.ha-1), mixture of Azotobacter, Azospirillum and Pseudomonas (inoculation and not-inoculation) and zeolite (0 and 4.5 t.ha-1) on essential oil content, composition and macro element contents of Pimpinella anisum. Experimental design was randomized complete blocks with factorial arrangement. Field experiment was conducted at Sanandaj Agriculture Research Station, in 2009. The results showed that vermicompost application improved essential oil content and yield, anethol and methyl chavicol percentage, nitrogen, phosphorus, and potassium content. Inoculating seeds with PGPR led to significant increment in all of the mentioned characters except for methyl cavicol percentage and potassium concentration. Zeolite application had significant effect on essential oil content, oil yield and anethol percentage but no significant effect on the other characters. The most favorable interaction treatment for essential oil production and phosphorus percentage was the PGPR inoculation combined with the highest level of vermicompost.
S. Mafakheri; R. Omidbaigi; F. Sefidkon; F. Rejali
Abstract
Dracocephalum moldavica L. is an annual species of the Lamiaceae family with local and global importance. The aerial parts of the plant containing essential oil used in medicinal, cosmetic and food industries. In order to study the effects of biofertilizers on quantity and quality of essential ...
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Dracocephalum moldavica L. is an annual species of the Lamiaceae family with local and global importance. The aerial parts of the plant containing essential oil used in medicinal, cosmetic and food industries. In order to study the effects of biofertilizers on quantity and quality of essential oil in Dragonhead including the content of the essential oil and the content of the geraniol, geranial and geranyl acetate in the essential oil, an experiment was conducted at Tarbiat Modarres University in 2009. The factors were vermicompost (0, 15, 30% V/pot), Azotobacter (inoculated and non-inoculated) and phosphatic biofertilizer (inoculated and non-inoculated). A factorial experimental design was applied in a randomized complete blocks with twelve treatments and three replications. Mean comparison was carried out using Tukey multiple range test at 5% level. Results showed that the highest essential oil content (0.74%) and geranyl acetate content in the essential oil (61.1%) were obtained at the third level of the Vermicompost treatment (30%). The highest geraniol and geranial content in the essential oil (24.2% and 18.2%) respectively were obtained with V15× B0 and V15×A0.
F. Hariri moghadam; S. Moharramipour; F. Sefidkon
Abstract
Two-spotted spider mite Tetranychus urticae Koch is one of the most well known polyphagous pests of crops in the world. Due to undesirable effects of synthetic pesticides such as expression of resistance in mites, this research was conducted to achieve alternative safe compounds to environment. Therefore, ...
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Two-spotted spider mite Tetranychus urticae Koch is one of the most well known polyphagous pests of crops in the world. Due to undesirable effects of synthetic pesticides such as expression of resistance in mites, this research was conducted to achieve alternative safe compounds to environment. Therefore, in this Research, repellent activity and persistence of two plant essential oils, including of Eucalyptus salmonophloia F. Muell and Eucalyptus kingsmillii (Mauden) Maiden & blakely, were studied on T. urticae , at 27 ± 0.5 °C, 50 ± 0.5% RH and photoperiod of 16:8 h (L:D). The essential oils were extracted using Clevenger-type apparatus. According to the repellency index formula, it was found that both essential oils of E. salmonophloia and E. kingsmillii from 4 applied concentrations were only repellent in two concentrations. Generally, no significant differences of repellency were found between two plant essential oils of E. salmonophloia and E. kingsmillii at different concentrations. In repellency experiment for essential oils, it was found that no significant differences were observed between essential oils of E. salmonophloia and E. kingsmillii. Our findings indicated that Eucalyptus species had reliable repellent activity and persistence on T. urticae. However, more research is necessary to make these compounds applicable.
Z. Aslani; A. Hassani; M. Rasooli Sadaghiyani; F. Sefidkon; M. Barin
Abstract
To study the influence of arbuscular mycorrhizal (AM) fungi inoculation and drought stress on growth, chlorophyll content and phosphorus uptake of basil (Ocimum basilicum L.), a pot experiment was conducted using a factorial based on randomized complete blocks design with four replications. The factors ...
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To study the influence of arbuscular mycorrhizal (AM) fungi inoculation and drought stress on growth, chlorophyll content and phosphorus uptake of basil (Ocimum basilicum L.), a pot experiment was conducted using a factorial based on randomized complete blocks design with four replications. The factors were mycorrhizal inoculation (non-inoculated and inoculated with Glomus mosseae T.H. Nicolson & Gerd. and Glomus intraradices N.C. Schenck & G.S. Sm) and three irrigation regimes (irrigation intervals were every 4, 8 and 12 days). The results showed that drought stress had significant effects on evaluated characteristics. As with decrease of the soil water content, plant height, stem diameter, number and area of leaves, number of axillary shoot, root dry weight, fresh and dry herb yield, chlorophyll content, P concentration in leaves and root colonization decreased. Also, AM fungi inoculation had significant effects on growth parameters and P uptake. Plants inoculated with AM fungi had higher growth, yield and P uptake than non-inoculated plants under droughtstress and non-stress conditions. G. mosseae was more effective than G. intraradices in alleviation of drought stress. It could be concluded that AM fungi are able to enhance the growth and yield of basil under drought stress condition through enhancing P uptake.
A. Salehi; A. Ghalavand; F. Sefidkon; A. Asgharzade
Abstract
In order to study the effects of zeolite, bio and organic fertilizers on N, P, K concentration, essential oil content and yield in organic cultivation of German Chamomile (Matricaria chamomilla), a factorial experiment in randomized complete blocks design with twelve treatments and three replications ...
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In order to study the effects of zeolite, bio and organic fertilizers on N, P, K concentration, essential oil content and yield in organic cultivation of German Chamomile (Matricaria chamomilla), a factorial experiment in randomized complete blocks design with twelve treatments and three replications was conducted in the faculty of agriculture, Tarbiat Modares University, 2005. The factors were PGPR inoculums (inoculated and no inoculated), zeolite (0 and 9 tons/ha) and vermicompost (0, 5, 10 tons/ha). Results showed that the highest concentration of N, P and K in plant, essential oil content and yield were obtained with PGPR inoculums. Zeolite also showed significant effects on the mentioned traits except P concentration. With application of zeolite 9 ton/ha, essential oil content, essential oil yield, N and K concentration were increased. Essential oil content, essential oil yield, N, P and K concentration were also increased with increasing of vermicompost levels as the highest values were obtained with application of 10 ton/ha vermicompost. There was positive and synergistic interaction between PGPR inoculums and vermicompost on N concentration as the highest and lowest N concentration percentage in herb were obtained in treatments of V3B2 (1.48) and V1B1 (1.12), respectively. According to the results, it appears that application of 10 tons vermicompost, inoculation with PGPR and 9 tons zeolite was the best treatment in production of German chamomile essential oil yield in organic system.
M. Naderi Hajibagher kandi; F. Sefidkon; A. Azizi; M.R. Pourheravi
Abstract
Laurus nobilis L. (Lauraceae) is an evergreen shrub widely distributed in the Mediterranean area and Southern Europe. Laurus nobilis is widely found in north of Iran and other places and its cultivation has been common due to evergreen leaves and beautiful appearance. Due to the relationship between ...
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Laurus nobilis L. (Lauraceae) is an evergreen shrub widely distributed in the Mediterranean area and Southern Europe. Laurus nobilis is widely found in north of Iran and other places and its cultivation has been common due to evergreen leaves and beautiful appearance. Due to the relationship between distillation time and oil yield and composition, doing research on optimum distillation time to obtain the best quality and quantity of essential oils with the lowest energy consumption has been always considered by scientists. In this study, the effect of different extraction times including 15, 30, 45, 60, 75, 90, 105 and 120 minutes on the essential oils content and composition of Laurus nobilis leaves was investigated. The leaves of laurus nobilis were collected in July 2009 from National Botanical Garden of Iran in Tehran, and dried in shade. The essential oil of leaves was extracted by hydro-distillation and analyzed by capillary gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The leaf oil yield based on dry weight in 15, 30, 45, 60, 75, 90, 105 and 120 minutes were respectively 1.35%, 1.92%, 1.97%, 2%, 2.37%, 2.49%, 2.58% and 2.60%. Statistical analysis showed no significant differences in oil yield of 90, 105 and 120 minutes extraction times. According to the results, with a view to quantity, 90 minutes was identified as the best extraction time for essential oil extraction in laurus nobilis. 1, 8-cineole and sabinene were identified as the major components of the leaf oil respectively within 15 minutes and after 45 minutes. α-terpinyl acetate remained constant in all extraction times.
Sh. Ne'mati; F. Sefidkon; M.R. Poorherave
Abstract
Thymus species are well known as medicinal plants because of having biological and pharmacological properties. Thymus daenensis is an endemic aromatic medicinal plant to Iran. Essential oil of Thymus daenensis is a rich source of thymol which gives high antimicrobial and antioxidant activity to this ...
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Thymus species are well known as medicinal plants because of having biological and pharmacological properties. Thymus daenensis is an endemic aromatic medicinal plant to Iran. Essential oil of Thymus daenensis is a rich source of thymol which gives high antimicrobial and antioxidant activity to this plant. In this research, the aerial parts of Thymus daenensis were collected at full flowering stage from Alborz Research Station in Karaj. For finding the effect of drying methods on essential oil yield and composition, the plant material were dried in four different conditions (oven 30°C, 40°C, 50°C and shade). After drying the samples, their essential oils were obtained by water distillation in three replications and were analyzed by GC and GC/MS. Oil yield and percentage of main components were statistically compared by Duncan's test in SAS software. Oil yields (w/w) of the oven-dried 30°C, 40°C, 50°C and shade-dried samples were 1.42%, 1.12%, 1.24% and 1.29%, respectively. Thymol (75.3%, 75.7%, 72.0% and 67.2%), allo-aromadendrene (5.7%, 5.2%, 5.4% and 3.9%), γ-terpinene (2.9%, 2.8%, 5.9% and 10.2%), p-cymene (4.2%, 4.1%, 5.1% and 5.5%) and carvacrol (2.2%, 4.1%, 1.6% and 2.4%) were identified as the main components in oven-dried 30°C, 40°C, 50°C and shade-dried samples, respectively. Statistical analysis showed a significant difference between oil yields of oven-dried 30°C sample with other samples. The highest percentage of thymol was obtained from oven-dried 30°C and 40°C that showed a significant difference with other drying methods.
N. Esfahanianfard; F. Sefidkon; Gh. Bakhshi Khaniki
Abstract
To investigate variations in the essential oil content and composition, leaves of three Eucalyptus species named E. melliodora, E. kingsmilli and E. dundasii were collected in the middle of four seasons during two years from two warm regions, Dezful and Shushtar in South of Iran. The essential oils were ...
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To investigate variations in the essential oil content and composition, leaves of three Eucalyptus species named E. melliodora, E. kingsmilli and E. dundasii were collected in the middle of four seasons during two years from two warm regions, Dezful and Shushtar in South of Iran. The essential oils were obtained by hydrodistillation and the oils were analyzed by GC and GC/MS. The maximum oil yield of E. melliodora was obtained in winter (3.1-3.9%) and spring (2.6-2.7%) from Shushtar samples, and in autumn (1.7-2.0%) from Dezful samples. The highest percentage of 1,8-cineole was found in winter and autumn in both regions. The maximum oil yield of E. kingsmilli was obtained in winter (2.8-3.1%) from Shushtar samples, and in authumn (2.7-3.2%) from Dezful samples. The highest percentage of 1, 8-cineole was found in winter and autumn in Shushtar and autumn, spring and winter in Dezful. The maximum oil yield of E. dundasii was obtained in winter (2.8-4.7%) from Shushtar samples, and in summer (0.9%) from Dezful samples. The highest percentage of 1, 8-cineole was found in spring and autumn in Shudhtar and spring in Dezful. There were also some variations in the amounts of other major and minor components. According to the results and to achieve a suitable quality and quantity of Eucalyptus essential oil, cultivation of E. melliodora in Shushtar and Dezful is recommended. For obtaining the highest oil yield and 1,8-cineole content from the mentioned Eucalyptus species, the best harvesting time for E. melliodora is spring and winter in Shushtar and autumn in Dezful. Cultivation of E. kingsmilli in Dezful is more suitable than that in Shushtar and the best harvesting time is spring and autumn in Shushtar and autumn in Dezful. Cultivation of E. dundasii is also recommended in Shushtar while not in Dezful due to its low yield and quality of essential oil. The best harvesting time for E. dundasii in Shushtar is winter (the highest oil yield) and spring (the highest 1, 8-cineole percentage).
A.R. Yavari; V. Nazeri; F. Sefidkon; M.E. Hassani
Abstract
In order to evaluate sثرثقشم ecological factors and morphological traits and also to determine ploidy levels and chemical composition of essential oil of Thymus pubescens Boiss. & Kotschy ex Celak two natural habitats, Bostan-Abad and Yam habitats, were chosen in East Azerbaijan province. To ...
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In order to evaluate sثرثقشم ecological factors and morphological traits and also to determine ploidy levels and chemical composition of essential oil of Thymus pubescens Boiss. & Kotschy ex Celak two natural habitats, Bostan-Abad and Yam habitats, were chosen in East Azerbaijan province. To study and evaluate quantitative morphological characters, five complete plants and flowering branches were collected from each locality at flowering stage for essential oil extraction. Seeds also were collected in seed production season to study seed characters and chromosome counting. Ecological data of both localities also were recorded. Vegetative and reproductive traits of each population were studied and essential oil of each locality was analyzed by combination of GC-FID and GC-MS. At least 10 mitotic cells of each population were studied to determine chromosome number and ploidy levels. Results revealed that specimens from “Yam” had the longest flowering stems, the largest leaves and flowers, and also the highest number of flowers per inflorescence. Chromosome count results showed that specimens from Bostan-Abad were hexaploid (2n = 6x = 90) and those of Yam were tetraploid (2n = 4x = 60). The total yield of oils based on dry weight was 1.3% and 2.2% (w/w) from Bostan-Abad and Yam, respectively. Thymol was the main valuable phenolic compound similar to most of the species of genus Thymus (Bostan-Abad: 57.6% and Yam: 44.9%).
S. Yousefzadeh; S.A.M. Modarres-Sanavy; F. Sefidkon; A. Asgarzadeh; A. ghalavand
Abstract
Dragonhead (Dracocephalum moldavica L.) is an annual plant that belongs to the (Labiate or Lamiaceae) family. The essential oil of dragonhead has natural antibacterial and antimicrobial substances and having wide usage in industries like pharmaceutical, dietary and many others. In this research, effects ...
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Dragonhead (Dracocephalum moldavica L.) is an annual plant that belongs to the (Labiate or Lamiaceae) family. The essential oil of dragonhead has natural antibacterial and antimicrobial substances and having wide usage in industries like pharmaceutical, dietary and many others. In this research, effects of different harvest times on content and composition of essential oil were studied in Research field at Khoy Agricultural Research Center in West Azarbaijan province. Dragonhead seeds from both landrace and modern cultivars were planted simultaneously. The aerial parts of plant were harvested in six stages. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by GC and GC-MS. According to the results, maximum and minimum essential oils percentage for both landrace and modern cultivars were respectively observed in full flowering (0.53) and yellow-maturity stage (0.07). In addition, there were some differences between oil compositions of these dragonheadaccessions at different harvest times. The major oil components of dragonhead were the geranial, geraniol and geranyl acetate. The highest and the lowest sum total amount of geranial and geraniol were obtained at the early flowering (65.1%) and yellow-maturity (44.9%) for the landrace cultivar. The largest amount of geranyl acetate (50.7%) was gained for the modern cultivar at the early flowering stage while the least one (28.1%) was recorded for landrace cultivar plants 26 days after flowering when 10 to 30 percent of the flowers remained. The results showed that different harvest times had significant effects on essential oil content and composition.
F. Sefidkon; A. Bahmanzadegan; M. Golipour; V. Mozafarian; S. Meshkizadeh
Abstract
The genus Bunium comprised of 14 species in Iran, two of them (B. wolfi and B. lurestanicum) are endemic. Among these species, only B. persicum is famous and used in medicinal and nutrition industries. In this research, two other species of Bunium named as B. cylindricum (Boiss.& Hohen.) Drude and ...
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The genus Bunium comprised of 14 species in Iran, two of them (B. wolfi and B. lurestanicum) are endemic. Among these species, only B. persicum is famous and used in medicinal and nutrition industries. In this research, two other species of Bunium named as B. cylindricum (Boiss.& Hohen.) Drude and B. rectangulum Boiss.& Hausskn., were studied. At first, different parts of these plants were collected from their habitats. Different parts were separated and then dried. The dried plant materials were subjected to hydro-distillation for obtaining the essential oils. The oils were analyzed by GC and GC/MS. Seventeen components were characterized in the oil of aerial parts of B. cylindricum at flowering stage. Germacrene D (31.2%), dill apiol (26.9%), E- caryophyllene (11.6%) and germacrene B (7.1%) were the main constituents. 20 compounds were identified in the seed oil of B. cylindricum with dill apiol (25.8%), E- caryophyllene (15.4%), globulol (12.2%), spathulenol (7.2%) and germacrene D (6.6%) as main components. 11 compounds were identified in the seed oil of B. rectangulum with dill apiol (63.3%), Germacrene D (22.4%) and E-caryophyllene (5.1%) as main components. 21 components were characterized in the flower oil of B. rectangulum. Germacrene D (36.7%), Dill apiol (11.1%), bicyclogermacrene (16.5%) and E-caryophyllene (15.9%) were the main constituents. The results showed that the essential oils of both species contained mainly sesquiterpens, but the percentages of these compounds were different.
O. Khorsandi; A. Hassani; F. Sefidkon; H. Shirzad; A.R. Khorsandi
Abstract
Water and soil salinity on the environmental agents limit plant growth and its productivity in Iran. Anise Hyssop (Agastache foeniculum kuntz.) is a perennial and aromatic herb plant, belonging to the Lamiaceae family. The essential oil of Anise Hyssop used in food industries, pharmacy, perfumery and ...
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Water and soil salinity on the environmental agents limit plant growth and its productivity in Iran. Anise Hyssop (Agastache foeniculum kuntz.) is a perennial and aromatic herb plant, belonging to the Lamiaceae family. The essential oil of Anise Hyssop used in food industries, pharmacy, perfumery and making soda. This experiment was conducted in a randomized complete blocks design with six salt treatments including 0 (control), 25, 50, 75, 100 and 125 mM NaCl and four replications in green house. Some parameters such as growth, yield and content and composition of essential oil were modulated. The results showed that salt stress had significant effects on estimated parameters. Salinity decreased plant height, number and length of axillary shoots, steam diameter, length of internodes, number and area of leaves, fresh and dry weight of leave and shoot, herbal yield and amount of essential oil in pot. In the composition of essential oil β-pinene, myrcene, anisaldehyd and β-bourbonene increased and amount of linalool and methyl chavicol decreased. Also high salinity (100 and 125 mM) destroyed plants.
A.R. Yavari; V. Nazeri; F. Sefidkon; M.E. Hassani
Abstract
Thymus migricus Klokov & Desj.-Shost.is one of the 14 species of genus Thymus L. which grows naturally in Iran. In this study, natural habitats of this species were identified during travels to different regions in East and West Azerbaijan provinces in the early of growth season in 2008. Five complete ...
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Thymus migricus Klokov & Desj.-Shost.is one of the 14 species of genus Thymus L. which grows naturally in Iran. In this study, natural habitats of this species were identified during travels to different regions in East and West Azerbaijan provinces in the early of growth season in 2008. Five complete plants were collected from five localities in order to study and evaluate quantitative morphological characters. To study seed character, inflorescences were collected at the time of seed production. Flowering stems were used to investigate essential oil quantitatively. Data of locations, vegetative and reproductive characters of every population and amount of essential oils of each location were recorded. The results were analyzed using cluster analysis method with SPSS software. Populations were clustered based on vegetative, reproductive characters and essential oil amount. All populations were classified into 3 groups. "Band", "Nazloo" and "Ghushchi" populations in a same group, "Heris" clustered separately. Finally "Jolfa" population was placed in independent group. Specimens from "Jolfa" and "Nazloo" showed longest flowering stems, largest leaves and flowers and also highest essential oil production, therefore it is recommended for breeding programs in cold regions of northwest Iran.
A.R. Yavari; V. Nazeri; F. Sefidkon; M.E. Hassani
Abstract
Genus Thymus (Lamiaceae) consists of about 250 species. Fourteen species grow naturally in Iran of which four are endemic. Different species of Thymus are used as tonic, carminative, digestive, antispasmodic, anti-inflammatory, antitussive, expectorant and for the cold treatment in Iranian traditional ...
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Genus Thymus (Lamiaceae) consists of about 250 species. Fourteen species grow naturally in Iran of which four are endemic. Different species of Thymus are used as tonic, carminative, digestive, antispasmodic, anti-inflammatory, antitussive, expectorant and for the cold treatment in Iranian traditional medicine. Chemical composition of essential oil of T. migricus Klokov & Desj.-Shost., a species grows in northwest of Iran, has been investigated in this paper. The aerial parts were collected from Ghushchi, Nazloo and Band regions from West Azerbaijan province in the middle of May 2008. Then air-dried flowering stems of the plant were submitted to hydrodistillation using a Clevenger-type apparatus according to the method recommended in British Pharmacopoeia. The essential oil was analyzed by combination of GC-FID and GC-MS. The total yields of oils based on dry weight were 1.5%, 1.7% and 1.4% (w/w) from Ghushchi, Nazloo and Band, respectively. Twenty, Eighteen and Twenty-one compounds were identified from Ghushchi, Nazloo and Band, respectively among which thymol (46.6-70.5%), γ-terpinene (6.2-16.7%) and p-cymene (4.0-6.2%) have been the three major and common constituents and also had high concentrations. With respect to high yield of essential oil and valuable phenolic compound in Nazloo specimen, it is suggested for medicinal applications.
Z. Dehghan; F. Sefidkon; Gh. Bakhshi Khaniki; R. Kalvandi
Abstract
Different species from Labiatea family are used as spices and fragrance in food or as medicine for treatment of viral or microbial diseases in many countries. Labiatea family contains 46 genus and 410 species. One of the valuable and medicinal species of this family is Ziziphora clinopodioides Lam. subsp. ...
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Different species from Labiatea family are used as spices and fragrance in food or as medicine for treatment of viral or microbial diseases in many countries. Labiatea family contains 46 genus and 410 species. One of the valuable and medicinal species of this family is Ziziphora clinopodioides Lam. subsp. rigida (Boiss.). This species contains essential oil with pulegone as major compound. In this investigation, the effect of some ecological factors on essential oil content and composition of Z. clinopodioides was studied. The aerial parts of Z. clinopodioides have been collected at flowering stage from four habitats in Hamedan and Kurdestan provinces. The major parameters like altitude, side and slope percentage, soil characteristics and other species of plant were determined. The plant materials have been subjected to hydro-distillation in three replications to obtain their essential oils. The essential oils were analyzed by GC and GC/MS. The results showed that ecological factors had significant effect (a=0.01) on oil yields. Identification of chemical constituents of the oils showed that main compounds (pulegone and 1,8-cineole) and minor compounds exist in different percentage in different habitats. Therfore, the ecological factors affected essential oil composition of Z. clinopodioides.
L. Moein Najafabadi; P. Owlia; S. Mousavi Nadoushan; I Rasooli; H. Saderi; F. Sefidkon; M.H. Salari
Abstract
Pseudomonas aeruginosa is an important opportunistic pathogen with many virulence factors. In this study, the effects of sub-MICs of three essential oils on alginate production, biofilm formation, swimming, twitching and adhesion in P. aeruginosa have been evaluated. The plants (Zataria multiflora Boiss.,Myrtus ...
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Pseudomonas aeruginosa is an important opportunistic pathogen with many virulence factors. In this study, the effects of sub-MICs of three essential oils on alginate production, biofilm formation, swimming, twitching and adhesion in P. aeruginosa have been evaluated. The plants (Zataria multiflora Boiss.,Myrtus communis L. and Eucalyptus camaldulensis Dehnh.) were dried in shadow and were hydro-distilled for 90 minutes. Minimal inhibitory concentrations (MIC) of essential oils were determined by macrodilution method. The virulence factors in the mucoid P. aeruginosa 8821M were determined in the presence of sub-MICs (1/2, 1/4 and 1/8) of essential oils. The MICs of essential oils against P. aeruginosa for Z. multiflora, M. communis and E. camaldulensis oils were obtained 64, 64 and 64µg/mL, respectively. The results showed that all oils at 1/2 and 1/4 MICs significantly reduced all tested virulence factors. At 1/8 MICs, Z. multiflora oil had significantly reduced virulence factors, but another oils had different effects. This study showed that sub-MIC levels of Z. multiflora, M. communis and E. camaldulensisessentialoils affected alginate production, biofilm formation, swimming, twitching and adhesion in P. aeruginosa and it is probable to use these medicinal plants for treating.
S. Davazdahemami; F. Sefidkon; M.R. Jahansooz; D. Mazaheri
Abstract
In order to evaluate the effects of water salinity on ajowan (Carum copticum L.), a completely randomized design was conducted in Esfahan province in 2007. Eight water salinity treatments (0.3-21 dS/m) with 3 replications were applied in the field. The seeds (at ripening) and foliage (initial flowering) ...
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In order to evaluate the effects of water salinity on ajowan (Carum copticum L.), a completely randomized design was conducted in Esfahan province in 2007. Eight water salinity treatments (0.3-21 dS/m) with 3 replications were applied in the field. The seeds (at ripening) and foliage (initial flowering) of ajowan were harvested. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. According to results seed and foliage oils percentage were 3.5-4.4 and 0.25-0.35 cc/100 g, respectively. Maximum seed and foliage oils yield were 8.3 and 2.4 cc/m2 and minimum were 3.5 and 1.5 cc/m2 in control and in 21 dS/m, respectively. Maximum biological and seed yields were 202.7 and 1367.6 and minimum were 96.7 and 739.5 gr/m2 in control and 21dS/m, respectively. The most important constituents of essential oil were: thymol (55.5% and 56.2%), γ-terpinene (22.5% and 26.9%), ρ-cymene (14.2% and 11.2%), limonene (1.9% and 0.5%) and carvacrol (0.3% and 1.4%), respectively.
