Changes in some biochemical characteristics of purple coneflower (Echinacea purpurea (L.) Moench) medicinal plant in response to planting date and soil flooding duration

Asadi Sanam, S.; Zavareh, M.; Pirdashti, H.; Sefidkon, F.; Nematzadeh, Gh.A.; Hashempour, A.

doi:10.22092/ijmapr.2015.101483

Document Type : Research Paper

Authors

¹ Ph.D. Student, Department of Agronomy, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

² Department of Agronomy, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

³ Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

⁴ Medicinal Plants Research Division, Research Institute of Forests and Rangelands, Tehran, Iran

⁵ Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

https://doi.org/10.22092/ijmapr.2015.101483

Abstract

This research was aimed to investigate the effect of planting date and soil flooding duration on some biochemical characteristics of purple coneflower (Echinacea purpurea (L.) Moench) in Sari region. The study was conducted in a RCBD based split plot with three replications in the Research Farm of the Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, in 2012. Experimental treatments included three planting dates (June 30, July 30 and August 29) and three soil flooding durations (without flooding as control, three and five-day flooding) which were considered as main and sub-plots, respectively. Malondialdehyde (MDA), total phenols and flavonoids contents, protein oxidation of the leaves, enzyme activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase (POD) and catalase (CAT), as well as percentage of DPPH free radical inhibition were determined. Results of this experiment showed a significant increment of malondialdehyde (MDA) content in purple coneflower leaf with the highest level in five-day flooding duration and in August 29 planting date. The highest decrease in total protein was found in the same planting data and flooding duration with 90 % decline than control.The highest activity of antioxidant enzymes of superoxide dismutase (SOD) and ascorbate peroxidase (APX) was recorded in the purple coneflowers leaves, cultivated in June 30, and flooded for three days, while the highest activity of peroxidase (POD) and catalase (CAT) were observed in plants cultivated in July 30. Five-day soil flooding markedly increased the total phenols and flavonoids content just in plants transplanted in June 30. In addition, the highest percentage of DPPH free radical inhibition was measured in plants subjected to continues five-day soil flooding and cultivated in August 29. In conclusion, it seems that the coneflower plant relatively showed a good tolerance to flooding stress.

Keywords

References

- فرهنگیان کاشانی، س. و ملک احمدی، ف.، 1388. اثر تنش غرقابی بر برخی از پارامترهای فیزیولوژی گیاه فلفل (Capsicum annuum L.). زیست‌شناسی دانشگاه آزاد اسلامی واحد گرمسار، 4(1): 52-43.

- Arbona, V., Hossain, Z., Lopez-Climent, M.F., Perez-Clemente, R.M. and Gomez-Cadenas, A., 2008. Antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus. Physiologia Plantarum, 132(4): 452-466.

- Blokhin, O., Virolainen, E. and Fagerstedt, K., 2003. Antioxidant oxidative damage and oxygen deprivation stress: a review. Annals of Botany,
91: 179-194.

- Blumenthal, M., Lindstrom, A., Lynch, M.E. and Rea, P., 2011. Herb sales continue growth-up 3.3% in 2010. HerbalGram, 90: 64-67.

- Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.

- Brand-Williams, W., Cuvelier, M.E. and Berset, C., 1995. Use of a free radical method to evaluate antioxidant capacity. Food Science and Technology, 28: 25-30.

- Chirkova, T., Novitskaya, V. and Blokhina, O.B., 1998. Lipid peroxidation and antioxidant systems under anoxia in plants differing in their tolerance to oxygen deficiency. Russian Journal of Plant Physiology, 45: 55-62.

- Dalby-Brown, L., Barsett, H., Landbo, A.R., Meyer A.S. and Molgaard, P., 2005. Synergistic antioxidative effects of alkamides, caffeic acid derivatives, and polysaccharide fractions from Echinacea purpurea on in vitro oxidation of human low-density lipoproteins. Journal of Agricultural and Food Chemistry, 53(24): 9413-9423.

- Demirci, B., Koşar, M., Demirci, F., Dinç, M., and Başer, K. H. C., 2007. Antimicrobial and antioxidant activities of the essential oil of Chaerophyllum libanoticum Boiss. et Kotschy. Food Chemistry, 105(4): 1512-1517.

- Du, G., Li, M., Ma, F. and Liang, D., 2009. Antioxidant capacity and the relationship with polyphenol and Vitamin C in Actinidia fruits. Food Chemistry, 113(2): 557-562.

- European Advisory Services., 2007. The Use of substances with nutritional or physiological effect other than vitamins and minerals in food supplements. Study undertaken for DG Sanco, European Commission. Service contract nr. Sanco/2006/E4/018, 82p.

- Giannopolitis, C. and Ries, S., 1977. Superoxide dismutase. I. occurrence in higher plant. Plant Physiology, 59(2): 309-314.

- Gutirrez Boem, F.H., Lavado, R.S.L. and Porcelli, C.A., 1996. Note on the effects of winter and spring waterlogging on growth, chemical composition and yield of rapeseed. Field Crops Research, 47: 175-179.

- Heath, R.L. and Packer, L., 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.

- In, B.C., Motomura, S., Inamoto, K., Doi, M. and Mori, G., 2007. Multivariente analysis of realation between preharvest environmental factors, postharvest morphological and physiological factors and vase life of cut Asomi Red Roses. Japanese Society for Horticultural Science, 76: 66-72.

- Jackson, M.B., Ishizawa, K. and Ito, O., 2009. Evolution and mechanisms of plant tolerance to flooding stress. Annals of Botany, 103(2): 137-142.

- Kopyra, M. and Gwozdz, E.A., 2003. Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus. Plant Physiology and Biochemistry, 41: 1011-1017.

- Kumutha, D., Ezhilmathi, K., Sairam, R.K., Srivastava, G.C., Deshmukh, P.S. and Meena, R.C., 2009. Waterlogging induced oxidative stress and antioxidant activity in pigeonpea genotypes. Biology of Plants, 53: 75-84.

- Letchamo, W., Polydeonny, L.V., Gladisheva, N.O., Arnason, T.J., Livesey, J. and wang, D.V.C.A., 2002. Factors affecting Echinacea quality. Trends in New Crops and New Uses, 514-521.

- Lin, K.H.R., Tsou, C.C., Hwang, S.Y., Chen, L.F.O. and Lo, H.F., 2006a. Paclobutrazol pre-treatment enhanced ﬂooding tolerance of sweet potato. Journal of Plant Physiology, 163: 750-760.

- Lin, K.H., Chao, P.Y., Yang, C.M., Cheng, W.C., Lo, H.F. and Chang, T.R., 2006b. The effects of flooding and drought stresses on the antioxidant constituents in sweet potato leaves. Botanical Studies, 47: 417-426.

- Luck, H., 1974. In: Methods in Enzymatic Analysis 2 (Ed Bergmeyer). Academic Press New York, 885P.

- Maclean, D., Murr, D.P., Deell, J.R. and Horvath C.R., 2006. Postharvest variation in apple (Malus domestica Borkh.) flavonoids following harvest, storage, and 1-MCP treatment. Agricultural and Food Chemistry, 54: 870-878.

- Meyers, K.J., Watkins C.B., Pritts M.P. and Hai-Liu, R., 2003. Antioxidant and antiproliferative activities of strawberries. Agricultural and Food Chemistry, 51: 6887-6892.

- Mittova, V., Guy, M., Tal, M. and Volokita, M., 2004. Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. Journal of Experimental Botany, 55: 1105-1113.

- Mrozikiewicz, P.M., Bogacz, A., Karasiewicz, M., Mikolajczak, P.L., Ozarowski, M., Seremak-Mrozikiewicz, A., Czerny, B., Bobkiewicz-Kozlowska, T. and Grzeskowiak, E., 2010. The effect of standardized Echinacea purpurea extract on rat cytochrome P450 expression level. Phytomedicine, 17: 830-833.

- Nakano, Y. and Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 22: 867-880.

- Nijveldt, R.J., Vannood, E., Vanhoorn, D.E.C., Boelens, P.G., Vannorren, K. and Vanleeuwen, P.A.M., 2001. Flavonoids: a review of probable mechanism of action and potential applications. American Journal of Clinical Nutrition, 74: 418-425.

- Pellati, F., Benvenuti, S., Magro, L., Melegari, M. and Soragni, F., 2004. Analysis of phenolic compounds and radical scavengin activity of Echinacea spp. Journal of Pharmaceuticalan Biomedical Analysis, 35: 289-301.

- Pennycooke, J.C., Cox, S. and Stushnoff, C., 2005. Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia × hybrida). Environmental and Experimental Botany, 53(2): 225-232.

- Perata, W., Armstrong, L.A.C. and Voesenek, J., 2011. Plants and ﬂooding stress. New Phytologist, 190: 269-273.

- Qiujie, D., Bin, Y.S., Xiao, Z. and Wang, Z., 1996. Flooding- induced membrane damage, lipid oxidation and activated oxygen generation in Corn leaves. Plant and Soil, 179: 261-268.

- Razali, N., Razab, R., Mat Junit, S. and Abdulaziz, A., 2008. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale L.). Food Chemistry, 111: 38-44.

- Sabra, A., Daayf, F. and Renault S., 2012a. Differential physiological and biochemical responses of three Echinacea species to salinity stress. Scientia Horticulturae, 135: 23-31.

- Sabra, A., Adam, L., Daayf, F. and Renault, S., 2012b. Salinity-induced changes in caffeic acid derivatives, alkamides and ketones in three Echinacea species. Environmental and Experimental Botany, 77: 234-241.

- Sharma, S.S. and Dietz, K.J., 2009. The relationship between metal toxicity and cellular redox imbalance. Trends in Plant Science, 14: 43-50.

- Shi, Q., Fei, D., Wng, X. and Wei, M., 2007. Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress. Plant Physiology and Biochemistry, 45: 542-550.

- Simova-Stoilova, L., Demirevska, K., Kingaton-Smith, A. and Feller, U., 2012. Involvement of the leaf antioxidant system in the response to soil ﬂooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Science, 183: 43-49.

- Stanisavijevic, I., Stojicevic, S., Velickovic, D., Veljkovic V. and Lazic, M., 2009. Antioxidant and antimicrobial activities of Echinacea (Echinacea purpurea L.) extracts obtained by classical and ultrasound extraction. Biotechnology and Bioengineering. Bioeng, 17(3): 478-483.

- Tang, B., Shang-zhong, X.U., Zou, X.L., Zheng, Y.L. and Qiu, F.Z., 2010. Changes of Antioxidative Enzymes and Lipid Peroxidation in Leaves and Roots of Waterlogging-Tolerant and Waterlogging-Sensitive Maize Genotypes at Seedling Stage. Agricultural Sciences in China, 9: 651-661.

- Tanou, G., Molassiotis, A. and Diamantidis, G., 2009. Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity. Environmental and Experimental Botany, 65: 270-281.

- Tavarini, S., DeglInnocenti, E., Remorini, D., Massai, R. and Guidi, L., 2008. Antioxidant capacity, ascorbic acid, total phenols and carotenoids changes during harvest and after storage of Hayward kiwifruit. Food Chemistry, 107: 282-288

- Unger, I.M., Motavalli, P.P. and Muzika, R.M., 2009. Changes in soil chemical properties with ﬂooding: a ﬁeld laboratory approach. Agriculture, Ecosystems and Environment, 131: 105-110.

- Yan, B., Dai, Q., Liu, X., Huang, S. and Wang, Z., 1996. Flooding induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Plant and Soil, 179: 261-268.

- Yanagawa, Y. and Komatsu, S., 2012. Ubiquitin/proteasome-mediated proteolysis is involved in the response to ﬂooding stress in soybean roots, independent of oxygen limitation. Plant Science, 185-186: 250-258.

- Yordanova, R.Y. and Popova, L.P., 2001. Photosynthetic response of barley plants to soil ﬂooding. Photosynthetica, 39: 515-520.

- Yordanova, R.Y., Christov, K.N. and Popova, L.P., 2004. Antioxidative enzymes in barley plants subjected to soil ﬂooding. Environmental and Experimental Botany, 51: 93-10.

Iranian Journal of Medicinal and Aromatic Plants Research

Changes in some biochemical characteristics of purple coneflower (Echinacea purpurea (L.) Moench) medicinal plant in response to planting date and soil flooding duration

References

References

Volume 31, Issue 2 - Serial Number 70
June 2015
Pages 315-331

Changes in some biochemical characteristics of purple coneflower (Echinacea purpurea (L.) Moench) medicinal plant in response to planting date and soil flooding duration

References

References

Volume 31, Issue 2 - Serial Number 70June 2015Pages 315-331

Volume 31, Issue 2 - Serial Number 70
June 2015
Pages 315-331