M.R. Jalali; H. Jafari; P. Owlia; N. Fallah; A. Davati
Abstract
Salmonellosis is one of the most common infections in developing countries. Antibiotics used for management, may led not only to drug-resistance, but also to some serious complications. Since antiquity medicinal plants have been used for treatment of diseases, Garlic is one of these agents with multiple ...
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Salmonellosis is one of the most common infections in developing countries. Antibiotics used for management, may led not only to drug-resistance, but also to some serious complications. Since antiquity medicinal plants have been used for treatment of diseases, Garlic is one of these agents with multiple effects i.e. antibacterial effects. Sixty rabbits of Dutch-polish race were randomly divided in three equal groups; contaminated with no treatment as the control group, contaminated with treatment by low and high-doses of garlic aqueous extract in the other groups. All rabbits were infected with 1011 CFU of S. typhimurium fourty-eight hours after contamination, garlic aqueous extract was used in treated-groups at 20mg/kg and 40mg/kg three times a day. Stool culture is performed at first before contamination and after 24, 48, 72, 96 and 168 hours of treatment. Bacterial colony count stool showed significant statistics in different days. A significant difference was also seen in colony count of treated groups with non-treated group (p<0.001). But there was no definite difference in stool colony count of treated groups (low dose & high dose). So garlic aqueous extract has antibacterial effects on S.typhimurium. Further studies are recommended for clinical usage of this agent.
Zh. Asadi Kavan; M. Ghorbanli; A. Sateei
Abstract
Drought stress provokes ROS production in plant cell chloroplasts and subsequently causes lipid membrane peroxidation and damage. Pimpinella anisum L. is one of the aromatic herbal plants which has great export value. The aim of this study was applying exogenous ascorbate in order to control oxidative ...
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Drought stress provokes ROS production in plant cell chloroplasts and subsequently causes lipid membrane peroxidation and damage. Pimpinella anisum L. is one of the aromatic herbal plants which has great export value. The aim of this study was applying exogenous ascorbate in order to control oxidative stress during drought tolerance. Changes of pigment content of leaves, total phenol compounds, malonedialdehyde (MDA) content were measured. In a pot study, drought stress introduced to treatments with 3 replicates based on 3 levels of field capacity (100, 60 and 25%) and ascorbate (1.4 mM) sprayed on them. Chlorophyll content and chlorophyll a/b ratio decreased with increasing in stress levels, while flavonoids and anthocyanins increased. Carotene and xanthophyll increased only in moderate stress level due to drought. Exogenous ascorbate increased chlorophylls and carotenoid content but decreased flavonoid and anthocyanin contents and had great effect on increasing phenol compound in all stress levels. MDA content remained relatively constant, but increased significantly in severe stress levels. Applying exogenous ascorbate led to decreasing metabolite. According to the results exogenous ascorbate could increase the ability of Pimpinella anisum in response to drought stress with different mechanisms and had protective effect against lipid peroxidation due to drought stress.
M. Ghorbanli; ْا. Bakhshi Khaniki; S Salimi Elizei; M. Hedayati
Abstract
In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: ...
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In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: 1) Control with irrigation equal to field capacity, 2) two third of field capacity, 3) one third of field capacity, 4) two third of field capacity along with 10 mM ascorbic acid, 5) two third of field capacity along with 1 mM ascorbic acid, 6) one third of field capacity along with 10 mM ascorbic acid, 7) one third of field capacity along with 1 mM ascorbic acid. Proline and soluble sugars levels in shoots and roots were significantly different for all treatments except for the treatment with 2/3 field capacity along with 10 mM ascorbic acid. Enzymes levels in 1/3 and 2/3 treatments were significantly different in both shoots and roots. One mM ascorbic acid along with different field capacity showed no reducing effect. However, 10 mM ascorbic acid especially in 2/3 field capacity could reduce the effect of water deficit through reduction in level of metabolites produced in response to low irrigation.
Identification, introduction, ecology and ethnobotany
. .; Narjes Yusefi; Farnosh fatahi
Abstract
Background and objectives: Recording ethnobotanical knowledge of medicinal plants preserves the scientific heritage of medicinal and aromatic plants. This unique and valuable knowledge has long been passed down from generation to generation but is eroded as societies modernize. ...
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Background and objectives: Recording ethnobotanical knowledge of medicinal plants preserves the scientific heritage of medicinal and aromatic plants. This unique and valuable knowledge has long been passed down from generation to generation but is eroded as societies modernize. In this regard, the present study was conducted to identify medicinal plants used by residents. It also identified cultures, methods of use, and the therapeutic context of the mentioned plants in Pasargad County. In this region, herbal medicines are common, especially with the Basri nomadic tribe.Methodology: To understand the factors affecting the differentiation and segregation of the region, such as ecological, cultural, historical, and land use conditions, a field visit was first conducted in the Pasargad region. Ethnobotanical information was obtained using a semi-structured questionnaire and interviews with knowledgeable people. A total of 101 informants participated in the study. The target areas for sampling were determined after dividing the region into two central parts (Saadshahr) and Madras Sulaiman based on local guides' information. Demographic information about people, such as education, gender, age group, and registered status, was considered. This research seeks local knowledge and traditional applications for identifying medicinal samples. In this regard, information was collected from native people of the region, attars, and knowledgeable people about traditional medicine and medicinal plants. The necessary interviews were conducted. To quantitatively examine the ethnobotanical information, the indigenous knowledge of medicinal plants was classified using software (Excel 2021). Quantitative indices used in ethnobotanical studies allow the selection of the most influential species of medicinal plants for conducting medicinal research. In this regard, a quantitative analysis of the ethnobotanical information of local knowledge of medicinal plants based on indicators including the relative frequency of citations (RFC), usage value (UV), cultural importance (CI), and the consensus index of informants (ICF) is carried out.Results: The collected plants belonged to 18 families, the most abundant of which were Lamiaceae, Apiaceae, Asteraceae, Amaryllidaceae, Rosaceae, Fabaceae, and Malvaceae families. The most common herbal medicine consumption method was boiled (30%), followed by infusion (30%) and cooking (15%). The most used medicinal parts of plants in this region were leaves and flowers, with 44% and 21%, respectively. According to this study, Shirazi thyme, Stachys inflate, and Prangos ferulacea had the highest relative frequency of citations (RFC). Plants with the highest index of cultural importance in the region included species that show their high importance in the culture of using medicinal plants. The region's local people used two species, Zataria multiflora and Matricaria chamomilla. Recorded diseases and traditional remedies from medicinal plants were classified according to the International Classification of Primary Care (ICPC-2). The ICF index was calculated in 9 disease classes based on the usage report and the number of species applied to each disease class. Thus, nine categories of diseases, including digestive (GAS-D), respiratory (RES-R), nervous (NER-N), skin and hair (DER-S), general and unspecified (OTH-A), urology and kidney (URO-U), endocrine/metabolic and nutrition (MET-T, gynecological diseases (GYN-X), cardiovascular (CAR-K) were determined. The highest amount of ICF is related to three groups of gastrointestinal diseases. It was respiratory and nervous with an ICF value (of 0.92).Conclusion: High ICF of digestive diseases is due to the high UR of Zataria multiflora, Carum copticum, Pistacia atlantica, Mentha longifolia, and Glycyrrhiza glabra for digestive disorders. The second rank of ICF of respiratory diseases is related to the high UR of Zataria multiflora, Achillea eriophora, and Stachys lavandulifolia. Also, the high UR of Matricaria chamomilla, Salvia officinalis, Rosa damaescena, and Ballota oucheri accounts for the high ranking. These findings are due to the high UR of medicinal plants to treat the diseases of the three mentioned ailments categories. This can have ecological and sociological reasons.
A. Mohammadian; R. Karamian; M. Mirza; A. Sepahvand
Abstract
Thymus is one of the most important genera of the Lamiaceae family, belonging to the Nepetoideae sub family. In this study, the relationship between altitude from sea level and soil physical and chemical characteristics as important factors affecting the quantitative and qualitative essential oils of ...
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Thymus is one of the most important genera of the Lamiaceae family, belonging to the Nepetoideae sub family. In this study, the relationship between altitude from sea level and soil physical and chemical characteristics as important factors affecting the quantitative and qualitative essential oils of Thymus fallax Fisch.et C.A. Mey.were examined. Four natural habitats of this species (Zagheh, Alashtar, Razan and Rhimeleh) were selected. Aboveground samples were collected in flowering stage and the essential oils were extracted with hydrodistilation method. Essential oils composition was determined using GC & GC/MS. Data analysis was performed by SPSS software, and Duncan's Multiple Range Test was used for mean comparison. According to the obtained results, the highest essential oil was recorded for Rhimeleh (2.8 %w/w) as compared to other habitats. The main components in the essential oils were carvacrol, thymol, gamma- terpinen, p-cymen, champhen and geranial. With increasing altitude, the percentage of carvacrol and thymol increased significantly. Some soil characteristics were correlated with the essential oils, presented in a linear regression model.
Agriculture and horticulture
S. Heidari; J. Sarhadi; M. Sharif
Abstract
To investigate the effects of organic and chemical fertilizers and their combination on the characteristics of henna (Lawsonia inermis L.) in Southern Kerman province, a factorial farm experiment was conducted in a randomized complete block design with three replications for two years. The factors included ...
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To investigate the effects of organic and chemical fertilizers and their combination on the characteristics of henna (Lawsonia inermis L.) in Southern Kerman province, a factorial farm experiment was conducted in a randomized complete block design with three replications for two years. The factors included rotten cattle manure at two levels (0 (a1) and 15 (a2) ton.ha-1) and NPK fertilizer (urea, triple superphosphate and potassium sulfate) at five levels (0 (b1), 25% (b2), 50% (b3), 75% (b4), and 100% (b5) of the soil test basis). At the end of each year, leaf yield and nitrogen, phosphorus, potassium, and lawson concentrations of the leaves were measured in the different treatments. The results of two-year combined analysis showed that the interaction between organic and chemical fertilizer treatments was significant on yield and leaf lawson concentration. The highest leaf dry weight (483.2 g.m-2) was related to the a2b5 treatment which was not significantly different from a2b4 (473.7 g.m-2). The a2b5 and a2b4 treatments had 41.2% and 38.5% better yield than the control, respectively. Also, the a2b5 treatment showed the highest leaf lawson content (30.1 g.plant-1) without significant difference with a2b4 (25.2 g.plant-1). Considering the situation of the study region in terms of non-use of organic fertilizers and also the economic justification of the produced henna, it could be recommended to use 15 ton.ha-1 cattle manure with a 25% reduction of chemical fertilizer NPK for the henna production.
Z. Habibi; M. Ghavidel; M. Yousefi
Abstract
In the current research, aerial parts of Salvia verticillata L. were collected during the flowering stage from Esfehan-Khor and its chloroform extract was investigated. Purification of crude extract was carried out by column chromatography on silica gel with a gradient of n-hexan-ethyl acetate. At the ...
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In the current research, aerial parts of Salvia verticillata L. were collected during the flowering stage from Esfehan-Khor and its chloroform extract was investigated. Purification of crude extract was carried out by column chromatography on silica gel with a gradient of n-hexan-ethyl acetate. At the end of chromatography, the column was eluted by methanol. Finally thirty-seven fractions were collected; further purification was carried out by column chromatography (smaller columns) and thin layer chromatography (glass plates) in n-hexane–ethyl acetate as solvent for several times and yielded pure compounds. The structure of purified natural products was elucidated by using Nuclear Magnetic Resonance (NMR) spectroscopy and Mass spectrometry. β-sitosterol and two diterpenoides namely Marrubinone B and Peregrinol were isolated.
S. Ghasemi; Z. Habibi; F. Rezaalizadeh Rooshan
Abstract
In this study, the chloroform extract of Ferula ovina (fam. Apiaceae) stems was investigated. The plants were collected at the flowering stage from Tehran province (Damavand), Iran. The extract was purified using column chromatography on silica gel with a solvent gradient of n-hexane-ethyl acetate and ...
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In this study, the chloroform extract of Ferula ovina (fam. Apiaceae) stems was investigated. The plants were collected at the flowering stage from Tehran province (Damavand), Iran. The extract was purified using column chromatography on silica gel with a solvent gradient of n-hexane-ethyl acetate and yielded 12 fractions. Further purification of the fractions resulted in the isolation and identification of four monoterpenoid ester derivatives named tschimgine (1), (1S,2R,4S)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl 4-methoxy benzoate (2), (1S,2R,4S)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl 4-hydroxy-3-methoxy benzoate (3) and stylosin (4). So far, no report has been found on the isolation and structure identification of the compound No.2. The structure of the compounds was determined by spectroscopic analysis andfinally confirmed by comparison of their spectral data, melting points, and optical rotations with those described in the literature.
R. Rezaei; S.A.R. Valadabadi; A.H. Shirani Rad; S. Sayfzadeh; E. Hadidi Masouleh
Abstract
In order to investigate the effects of biological fertilizers application and various amounts of urea fertilizers under the water stress conditions on yield, nitrogen use efficiency, and effective ingredients of Calendula officinalis L., an experiment was implemented during the two cultivation years ...
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In order to investigate the effects of biological fertilizers application and various amounts of urea fertilizers under the water stress conditions on yield, nitrogen use efficiency, and effective ingredients of Calendula officinalis L., an experiment was implemented during the two cultivation years of 2015-2106 and 2016-2017. The study was conducted at the research farm of Islamic Azad University, Varamin-Pishva Branch. The experiment was performed in three replications as a split-split plot in a completely randomized blocks design. The experimental treatments included two levels of water stress as (the main factor): the absence of water stress (control) and irrigation cut off at the budding stage, as well as, four levels of biological fertilizers (as sub-factor) (no use), the application of Azotobacter (Chroococum), the application of Azospirillum (Brasilense), and combined application of Azotobacter and Azospirillum. Urea fertilizer sources were also classified (sub-factor) in four levels including no use (control), recommended amount of sulfur coated urea fertilizer (175 kg/ha of recommended amount of fertilizer), 75% of recommended amount of sulfur coated urea fertilizer (131 kg/ha), and the recommended amount of urea fertilizer without coating at 175 kg/ha. The results showed that irrigation cut off at budding stage resulted in reduced flower yield, biological yield, the amount of nitrogen, nitrogen use efficiency (crop efficiency), and nitrogen uptake efficiency. However, it resulted in the increased nitrogen use efficiency, α-cadinol, and morolol. In addition, in both years of experiment, the combined use of Azotobacter and Azospirillum, along with the recommended amount of sulfur coated urea fertilizer resulted in the increased content of α-cadinol. The highest nitrogen use efficiency was achieved under non-water stress conditions and the combined use of bio-fertilizer (Azotobacter and Azospirillum) and the use of 75% of the recommended amount of sulfur coated urea fertilizer. In the second year, the lack of water stress and the combined use of bio-fertilizer (Azotobacter and Azospirillum) and the recommended amount of sulfur coated urea fertilizer also resulted in a significant reduction in nitrogen use efficiency. In the first and second years, the lack of water stress and the combined use of Azotobacter and Azospirillum and the recommended amount of sulfur coated urea fertilizer resulted in increased flower yield and biological yield.
Sh. Khalesro; A. Ghalavand; F. Sefidkon; A. Asgharzadeh
Abstract
Anise (Pimpinella anisum L.) is one of the aromatic and medicinal plants. Anethol is the most important component of its essential oil used in medicinal and food industries. The aim of this experiment was to determine the effect of vermicompost levels (0, 5 and 10 t.ha-1), mixture of Azotobacter, Azospirillum ...
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Anise (Pimpinella anisum L.) is one of the aromatic and medicinal plants. Anethol is the most important component of its essential oil used in medicinal and food industries. The aim of this experiment was to determine the effect of vermicompost levels (0, 5 and 10 t.ha-1), mixture of Azotobacter, Azospirillum and Pseudomonas (inoculation and not-inoculation) and zeolite (0 and 4.5 t.ha-1) on essential oil content, composition and macro element contents of Pimpinella anisum. Experimental design was randomized complete blocks with factorial arrangement. Field experiment was conducted at Sanandaj Agriculture Research Station, in 2009. The results showed that vermicompost application improved essential oil content and yield, anethol and methyl chavicol percentage, nitrogen, phosphorus, and potassium content. Inoculating seeds with PGPR led to significant increment in all of the mentioned characters except for methyl cavicol percentage and potassium concentration. Zeolite application had significant effect on essential oil content, oil yield and anethol percentage but no significant effect on the other characters. The most favorable interaction treatment for essential oil production and phosphorus percentage was the PGPR inoculation combined with the highest level of vermicompost.
M. Talezade; A. Nezami; M. Parsa; J. Nabati; R. Tavakkol Afshari
Abstract
Domestication, cultivation and crop production of Bunium persicum (Boiss.) B. Fedttsch. are important to preserve genetic resources of the species. In this regard, in order to identify the proper medium and nutrient solution for the seedling establishment of B. persicum, a factorial experiment in a completely ...
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Domestication, cultivation and crop production of Bunium persicum (Boiss.) B. Fedttsch. are important to preserve genetic resources of the species. In this regard, in order to identify the proper medium and nutrient solution for the seedling establishment of B. persicum, a factorial experiment in a completely randomized design was carried out under greenhouse conditions with three replications. Experimental factors consisted of culture medium at three levels of cocopeat, sand and soil, and Hoagland solution in five concentrations of 0, 25, 50, 75, and 100%. The results showed that the highest seedling emergence percentage (37.7) was observed in cocopeat without Hoagland solution. The highest seedling vigor index (773) and also green area of seedling (2.14 cm2/plant) and the lowest seedling vigor index (205) were observed in cocopeat and soil media (both with 75% Hoagland solution), respectively. Maximum tuberization percentage (100) was recorded in the sand with 50% Hoagland solution. The highest tuber weight (76.0 mg) and volume (79.2 mm3) were obtained in the sand with 75% Hoagland solution without any significant difference with the results of sand with 50% Hoagland solution treatment. Generally, the results of this study indicated that using a medium with suitable porosity and water holding capacity and also irrigation with at least 50% Hoagland solution can improve seedling emergence percentage, seedling establishment, and production of tuber with appropriate size in B. persicum.
M. Moosavian; M. Darvishnia
Abstract
This research was aimed to study the effects of antifungal activity of cloves (Syzygium aromaticum L.) essential oil against three pathogens-Botrytis cinerea, Alternaria alternata and Fusarium solani-isolated from tomato, cherries and potatoes by adding the fungus to their medium and also identify antifungal ...
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This research was aimed to study the effects of antifungal activity of cloves (Syzygium aromaticum L.) essential oil against three pathogens-Botrytis cinerea, Alternaria alternata and Fusarium solani-isolated from tomato, cherries and potatoes by adding the fungus to their medium and also identify antifungal activity of the essential oil as a food preservative against the pathogen. Essential oil was extracted by Clevenger-type apparatus and identification and amount of the essential oil was performed using chromatography–mass spectroscopy and gas chromatography.The most important components of the essential oil were isoeugenol (87.09%), eugenol acetate (9.71%), Aromadendrene (0.68%), Chavicol (0.3%), and Linalool (12.51%), respectively. Isoeugenol has the highest percentage of growth inhibitory on B. cinerea, so that, on average, 97% of the growth of this fungus was prevented at the concentration of 200 µl/l. However, the growth inhibitory percentage at this concentration was calculated to be 87% and 94%, for A. alternata and F. solani, respectively. More than 95% and 100% of the growth of all three fungi was prevented at concentrations of 500 and 1000 µl/l of essential oil.The best essential oil concentration to prevent the growth of fungi on fruit tissue was 200µl/l for B. cinerea, and 1000 µl/l for two other fungi. Antifungal properties of S. aromaticum essential oil are due to phenolic compounds in it like eugenol. Our results clearly showed that S. aromaticum essential oil was effective against post-harvest diseases, and its antiseptic properties could be used in plant pathology.
Agriculture and horticulture
Z. Ebrahimi; M.R. Morshedloo; M.B. Hassanpour Aghdam
Abstract
To investigate the effects of different salinity levels on the essential oil content, and some morphological and physiological characteristics of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.), a factorial greenhouse experiment was ...
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To investigate the effects of different salinity levels on the essential oil content, and some morphological and physiological characteristics of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.), a factorial greenhouse experiment was conducted in a completely randomized design with four treatments and three replications. The salinity stress treatment was applied using sodium chloride in Hoagland nutrient solution at four levels of control (no stress), and mild (salinity 30 mM), moderate (salinity 60 mM), and severe (salinity 90 mM) stresses. According to the results, under salinity stress the growth indices, total carotenoids, and chlorophyll index decreased, but the activity of antioxidant enzymes and concentration of malondialdehyde and hydrogen peroxide increased in both subspecies. The wet and dry weight, carotenoids, hydrogen peroxide, and guaiacol peroxidase activity were obtained higher in subsp. hirtum than subsp. gracile. The percentage and essential oil composition were also affected by salinity. With increasing the salinity levels, the essential oil percentage was significantly increased compared to the control and its highest amount (0.8%) was observed under mild stress. Thirty five compounds were identified in both oregano subspecies essential oil. Carvacrol (30-72%: subsp. hirtum crarvacrol more than the other subsp.), γ-terpinene, carvacrol methyl ether, and p-cymene were the main components of both subspecies. The results of this study demonstrated the superiority of subsp. hirtum to the other oregano subspecies in terms of the tolerance to salinity stress and also quantitative and qualitative yields.
R. Shahhoseini; M. Moghaddam; D. Kiani; R. Mansori
Abstract
Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD ...
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Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD (Randomized Complete Block Design) with six treatments (0, 1000, 2000, 3000, 4000 and 5000 mg l-1) and four replications in order to evaluate the effects of different concentrations of IBA and NAA on rooting of semi-hardwood cuttings of rosemary. Cuttings were treated in solutions of IBA and NAA for 1 min and held in plastic tunnel under mist system. After 70 days, the percentage of rotting, number of roots, average root length, the root fresh and dry weight, length of cutting, leaves and stem fresh and dry weight were measured. The analysis of variance showed that application of IBA and NAA had a significant effect on all the parameters studied. The highest rooting percentage was obtained at concentration of 1000 mg l-1 NAA (84%) and 5000 mg l-1 IBA (66%). The concentration of 4000 mgl-1 IBA was the best treatment on quality and rooting of the semi-hardwood cuttings of rosemary.
F. Shahbazi Asl; A.A. Jafari; H. Panahpour
Abstract
In order to study the biomass yield in flowering stage and morphological traits in Mentha spp., 17 accessions of M. pulegium L. and 8 accessions of M. mozaffariani Jamzad were assessed in a field experiment over two years in the Alborz research station, Karaj Iran. The accessions were planted using ...
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In order to study the biomass yield in flowering stage and morphological traits in Mentha spp., 17 accessions of M. pulegium L. and 8 accessions of M. mozaffariani Jamzad were assessed in a field experiment over two years in the Alborz research station, Karaj Iran. The accessions were planted using a complete randomized blocks design in two replications. Each plot contained 15 spaced plants with 0.5 m intervals and 75 cm between rows. The drip irrigation system was used. Data were collected for fresh and dry matter yield over two years and plant height, stem number, number of flowers per plant, the canopy area, and flower length for one year. Data were analyzed, and species means were compared based on DMRT Method. The results showed significant difference between the species for all traits except dry matter yield (P<0.01). In comparisons between species, M. pulegium had higher values of all traits except flower length than that for M. mozaffariani. For fresh yield, M. pulegium with an average value of 183 g/plant had significantly higher production as compared with M. mozaffariani with an average value of 147 g/plant. There were significant differences among accessions for all traits in both species (P<0.01). In comparisons among the accessions of M. pulegium, the accessions Rafsanjan (23031) and Maryvan (22607) with the average of 252 and 231 g/plant had higher fresh yield over two years, respectively. For dry mater yield, Rafsanjan (23031) and Arak (22381) with average values of 115 and 111 g/plant had higher dry matter production, respectively. In comparisons among the accessions of M. mozaffariani, Urmia (19625) with average values of 202 and 98 g/plant had higher fresh and dry matter production, respectively. For essential oil percentage, there were no significant differences between two species; however, there was a significant difference for oil yield. M. pulegium with an average value of 1.14 g/plant had higher oil production than that for M. mozaffariani with an average value of 0.76 g/plant.
B. Mosallanejad; R. Avizeh; H. Najafzadeh Varzi; M. Pourmehdi
Abstract
In the present study, effect of silymarin was evaluated as a protective drug of liver against acute hepatotoxicity due to administration of mebendazole in German shepherd dogs (mixed breeds). Twenty five healthy dogs were randomly allotted to five equal groups. Dogs in group A were given mebendazole ...
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In the present study, effect of silymarin was evaluated as a protective drug of liver against acute hepatotoxicity due to administration of mebendazole in German shepherd dogs (mixed breeds). Twenty five healthy dogs were randomly allotted to five equal groups. Dogs in group A were given mebendazole with single dose 150 mg⁄kg, p.o.; group B consisted of dogs that received silymarin with single dose 30 mg⁄kg, p.o. concurrent with mebendazole administration; groups C, D and E were treated like group B, but silymarin was administered 2, 12 and 24 h after administration of mebendazole respectively. The serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and total and direct bilirubin were measured before administration of mebendazole and 2, 12, 24 and 72 h later as indices of liver injury. A single oral administration of mebendazole significantly elevated serum concentrations of ALT, AST, ALP, LDH in all cases of group A (P<0.05), after 24 h. In both groups of B and C, levels of serumenzyme activities remained within the normal values. The difference was significant between groups B and C with group A (P<0.05). Levels of serumenzyme activities were higher than normal values in three cases of the group D and in all dogs of the group E. This study showed that silymarin could protect liver tissue against oxidative stress in dogs with mebendazole intoxication particularly in the first 2 hours after exposure.
M. Khosravi; S.Gh. Moosavi; M.J. Seghatoleslami
Abstract
In order to study the effect of irrigation interval, Nitrogen (N) fertilization and plant density on yield and yield components of fenugreek (Trigonella foenum-graecum L.), a split-split-plot experiment was conducted in Darmian, Iran in Spring 2010 based on a randomized complete block design with three ...
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In order to study the effect of irrigation interval, Nitrogen (N) fertilization and plant density on yield and yield components of fenugreek (Trigonella foenum-graecum L.), a split-split-plot experiment was conducted in Darmian, Iran in Spring 2010 based on a randomized complete block design with three replications. The main plots were irrigation interval at two levels of 5 and 10 days. The sub-plots were N fertilization at three rates of 0, 75 and 150 kg N.ha-1 from urea source and the sub-sub-plots were plant density at three levels of 22, 33 and 66 plants.m-2. According to the results of analysis of variance, irrigation interval significantly affected traits of pod length and water use efficiency (WUE) for seed and biomass production. In addition, main branch number per plant, pod length, seed yield, chlorophyll index and WUE for seed and biomass production, were significantly affected by nitrogen rate and plant density had significant effect on main branch number per plant and ring diameter. Means comparison showed that as irrigation interval was increased from 5 to 10 days, pod length decreased 8.7%, but WUE for seed and biomass production increased 40.5 and 47.5%, respectively. Also, as N rate was increased from 0 to 150 kg N.ha-1, main branch number per plant, pod length, seed yield, chlorophyll index and WUE for seed and biomass production increased by 23.5, 26.4, 23.2, 67.1, 72.3 and 71.5%, respectively. Moreover, with increasing of density from 22 to 66 plant. m-2, branch number per plant and ring diameter decrased 7.9 and 11.2%, respectively. In total, given the results of the study and the importance of water saving, it is recommended to use irrigation interval of 10 days with the application of 150 kg N.ha-1 and density of 66 plants.m-2 for the cultivation of fenugreek in Darmian, Iran.
Sh. Merikh; M. Taherkhani; S.R. Hosseini Doust
Abstract
The loganberry (Rubus × loganobaccus), belonging to the Rosaceae family and Rubus genus, gives fruits like strawberry. The fruit is red and very sour and this rare species is one of the cultivars of red raspberry. Loganberry is not native to Iran and currently is propagated through in vitro plant ...
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The loganberry (Rubus × loganobaccus), belonging to the Rosaceae family and Rubus genus, gives fruits like strawberry. The fruit is red and very sour and this rare species is one of the cultivars of red raspberry. Loganberry is not native to Iran and currently is propagated through in vitro plant tissue culture. So far, phytochemical research has not been conducted on loganberry. In present study, the leaf extract of loganberry, propagated through in vitro culture, was investigated phytochemically. The extract of loganberry was purified and the obtained natural product was elucidated by NMR spectroscopy techniques. In this study, a flavonoid, namely hesperetin, was isolated from the leaves of the loganberry. This structure was elucidated by spectral methods (1H-NMR, 13C-NMR, IR and dept 135).
M. Rajabi Hashjin; A. Asghari; M. Zeinalabedini; M.R. Ghaffari
Abstract
Fenugreek (Trigonella L.) is one of the most important and oldest known medicinal plants in the world, used in the treatment of many diseases due to its various secondary metabolites. The plant has more than 135 species growing throughout the world and in most climates. Trigonelline is the most important ...
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Fenugreek (Trigonella L.) is one of the most important and oldest known medicinal plants in the world, used in the treatment of many diseases due to its various secondary metabolites. The plant has more than 135 species growing throughout the world and in most climates. Trigonelline is the most important and most abundant alkaloid and one of the valuable metabolites found in fenugreek that is used to treat diabetes and reduce blood lipids. In this research, 22 genotypes from 10 species of fenugreek including: T. uncata, T. monantha, T. persica, T. anguina, T. stellate, T. spruneriana, T. astroites, T. monspeliaca, T. tehranica and T. foenum-graecum were collected from Khuzestan, Tehran, and Hormozgan provinces and their trigonelline levels were measured using HPLC technique. The results showed that T. foenum-graecum and T. tehranica had higher trigonelline content (1.34 and 0.9 mg/g seed, respectively) than other species. Also, there was a significant difference between genotypes for trigonelline content. The clustering based on Ward’s method separated genotypes into two groups. This study could be the basis for further work on the extraction of pharmaceutical compounds from other fenugreek species, especially native species of Iran such as T. tehranica.
Z. Dustiy; H. Abbasipour Shoushtari; A. Askarianzadeh
Abstract
The safflower fly, Acanthiophilus helianthi (Rossi) (Diptera: Trypetidae), is one of the most important pests of safflower in the world, causing a lot of damage to this crop every year. In Iran, the seed yield loss due to the safflower fly is estimated to be 30- 70 % for different safflower cultivars. ...
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The safflower fly, Acanthiophilus helianthi (Rossi) (Diptera: Trypetidae), is one of the most important pests of safflower in the world, causing a lot of damage to this crop every year. In Iran, the seed yield loss due to the safflower fly is estimated to be 30- 70 % for different safflower cultivars. The use of parasitoids is one of the pest management methods. In this study, the interaction between time and cultivar on the parasitism rate of safflower fly by two parasitoid species of Microdontromerus annulatus and Ormyrus gratiosus was investigated in the research field of Shahed University (south of Tehran). The study was conducted under a Completely Randomized Block Design with four replications. The treatments consisted of seven safflower cultivars including Zarghan, Padideh, Goldasht, Varamin, Pourdad PI, Acataria, Mec163. Sampling was performed every seven days and data analysis was done by the Kruskal Valis method. The highest percentage of parasitism by O. gratiosus on 19 July was observed on Mec163 and Varamin cultivars (12.35%). The lowest percentage of parasitism by this species was observed on 14 June in all cultivars. The highest parasitism rate by M. annulatus during the sampling period was observed on Padideh (7.56%) and Mec163 (7.52%) cultivars. The lowest percentage of parasitism (49.6%) by M. annulatus was observed on Varamin cultivar.
Biological effects of essential oils and extracts
Z. Ahmadi; D. Khademi Shurmasti
Abstract
Today, the tendency has increased to the use of bio-preservatives, including plant extracts, as an alternative to synthetic antioxidants and antimicrobials. On the other hand, the addition of lipids, such as oleic acid to biopolymers in active packaging reduces their permeability to water vapor. To investigate ...
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Today, the tendency has increased to the use of bio-preservatives, including plant extracts, as an alternative to synthetic antioxidants and antimicrobials. On the other hand, the addition of lipids, such as oleic acid to biopolymers in active packaging reduces their permeability to water vapor. To investigate the antioxidant and antimicrobial effect of Mentha spicataL. extract in bio-composite carboxymethyl cellulose-based coating on the shelf life of common carp fillets in cold conditions, an experiment was conducted in a completely randomized design with six treatments including carboxymethyl cellulose coating (CMS, 2.5%), CMS combined with oleic acid (OA, 1.5%), CMS combined with OA and enriched with 0.5, 1, 1.5 and 2% Mentha spicataextract, and three replications for 9 days. The results showed that OA improved the protective effect of CMS. The use of Mentha spicata extract in the bio-composite coating, especially at the highest concentrations, significantly (p < /em> <0.05) reduced the number of psychrotrophic bacteria, the index of thiobarbituric acid, free fatty acids, total volatile nitrogen compounds, and pH of common carp fillets during cold storage. The antioxidant and antimicrobial effect of Mentha spicataextract was dose-dependent. Therefore, Mentha spicata extract, with its antioxidant and antimicrobial properties in combination with the composite coating of CMS-OA, can be used as a bio-preservative in active packaging, to maintain quality and increase the shelf life of fish fillets during short-term storage in refrigeration.
S. Sarani; M. Heidari; M. Glavi; B. Siahsar
Abstract
To evaluate the effects of salinity in the presence and absence of iron on growth, photosynthesis pigments and electrophoresis bands in two chamomiles, an experiment was conducted as completely randomized factorial design with three replicates at university of Zabol in 2009. The treatments were two genuses ...
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To evaluate the effects of salinity in the presence and absence of iron on growth, photosynthesis pigments and electrophoresis bands in two chamomiles, an experiment was conducted as completely randomized factorial design with three replicates at university of Zabol in 2009. The treatments were two genuses of chamomile (German and Roman chamomile), four levels of salinity (0, 50, 100 and 150 mM NaCl) and two levels of iron (0 and 100 μmol). Results showed that, by increasing salinity from 0 to 150 mM NaCl, fresh and dry weight of shoot were reduced but root dry weight was added. At the absence of iron treatment, fresh and dry weight of both shoot and root were reduced in two genus of chamomile. Salinity decreased the amount of two pigments chlorophyll a and b and increased the amount of carotenoids. At the absence of iron, the amount of chlorophyll ‘a’ (32/9) and chlorophyll ‘b’ (35/5) percent decreased in comparison to control treatment (100 μmol). Unlike chlorophyll, at the absence of iron treatment, the amount of carotenoid was added. At the salinity stress from 0 to 150 mM, many protein bands in Roman chamomile were excluded. However, at the levels of 50 and 100 mM NaCl, a 42 KDa fraction band in this material was synthesized, but at the highest level of salinity (150 mM NaCl) many of their bands were removed. Presumably, the 150 mM NaCl is out of tolerance of Roman chamomile genotype. In this study, at the German chamomile genotype under salinity and iron deficiency no bonds were not removed and two bands 14 and 18 KDa were appeared.
Agriculture and horticulture
A. Koushki; A. Alinejadian-Bidabadi; A. Maleki
Abstract
To investigate the effects of rice husk biochar application on the various physiological and biochemical characteristics (fresh and aerial parts dry weight, plant height, percentage and essential oil yield,and content of chlorophylls, carotenoids, nitrogen, phosphorus, and potassium) of peppermint ...
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To investigate the effects of rice husk biochar application on the various physiological and biochemical characteristics (fresh and aerial parts dry weight, plant height, percentage and essential oil yield,and content of chlorophylls, carotenoids, nitrogen, phosphorus, and potassium) of peppermint (Mentha piperita L.) under different irrigation regimes, a factorial pot experiment with different levels of biochar (0, 3, and 6 t.ha-1) and irrigation (100% of full irrigation (control), 60, 80, and 120% of full irrigation) was conducted in a completely randomized design with four replications in the research greenhouse of Lorestan University in 2019. The results showed that the highest and lowest of aerial parts fresh weight were obtained in the 3 t.ha-1 biochar + 120% of full irrigation (24.2 g.pot-1) and 3 t.ha-1 biochar + 60% of full irrigation (13.3 g.pot-1) treatments, respectively. At the 60% of full irrigation level, the dry weight, height, essential oil yield, and N and P concentration of the plant aerial parts significantly decreased by 39.8, 33.3, 26.2, 17.2, and 8.7%, respectively compared to the control; while the essential oil percentage and K concentration of aerial parts had an increasing trend. The amount of a, b, and total chlorophyll, and carotenoids decreased under the water stress conditions. The highest amount of total chlorophyll was observed in the 6 t.ha-1 biochar + 100% of full irrigation treatment. The application of 6 t.ha-1 biochar increased the fresh and aerial parts dry weight, plant height, percentage and essential oil yield, photosynthetic pigments, and N, P, and K concentration of aerial parts compared to the no biochar application, significantly. The results showed that the 6 t.ha-1 biochar + 120% of full irrigation treatment increased most of the traits studied. However, due to the importance of peppermint in terms of essential oil production, the highest essential oil percentage was obtained at the highest level of water stress.
S. Morady; M. Pouryousef; B. Andalibi
Abstract
Foliar application of micronutrients is a useful technique for rapid absorption of minerals in the plants and avoids soil contamination. To evaluate the effect of foliar application of micronutrients (iron and zinc) on yield, yield components, and essential oil of fennel (Foeniculum vulgare Mill.), an ...
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Foliar application of micronutrients is a useful technique for rapid absorption of minerals in the plants and avoids soil contamination. To evaluate the effect of foliar application of micronutrients (iron and zinc) on yield, yield components, and essential oil of fennel (Foeniculum vulgare Mill.), an experiment was conducted as factorial based on randomized complete blocks design with three replications at the Faculty of Agriculture, University of Zanjan during the growing season of 2012. In this experiment, the effect of foliar application of Fe (using ferrous sulphate) at four concentrations (0, 1, 2 and 3 gr.L-1) and foliar application of Zn (using zinc sulphate) at three concentrations (0, 2 and 4 gr.L-1) were investigated. Results showed that the majority of measured traits including number of umbels per plant, 1000-seed weight, grain yield, essential oil content and essential oil yield were significantly affected (p≤ 0.01) by combined application of iron and zinc. Therefore, the highest grain yield (2637.96 kg ha-1) was obtained in foliar application of Fe + Zn at a concentration of 0.2%. In addition, the highest essential oil content (2.88%) and essential oil yield (75.71 kg ha-1) were obtained in combined application of Fe 0.3% and Zn 0.2% and the lowest content of mentioned traits was obtained in control.
M. Movahedi; V. Ghasemiomran; S. Torabi
Abstract
The present study was carried out to investigate the possibility of micro-propagation and to determine the optimal medium composition and combination of Cannabis sativa L. growth regulators under in vitro conditions. Seeds were surface-sterilized and then cultured on MS basal medium. One month later, ...
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The present study was carried out to investigate the possibility of micro-propagation and to determine the optimal medium composition and combination of Cannabis sativa L. growth regulators under in vitro conditions. Seeds were surface-sterilized and then cultured on MS basal medium. One month later, leaf and hypocotyl explants, obtained from the seedlings grown at in vitro condition, were used in MS culture medium containing NAA hormone (0.5, 1, 2 and 3 mg/l) either alone or in combination with 0.5mg/l BA; and 2,4-D (0.1, 0.2, 0.5 and 1 mg/l) alone or in combination with 0.5mg/l BA. Callus formation was the response of explants in most media. Direct shoot regeneration from explants was not observed but shoot induction from callus was seen only in 0.1 mg/L 2,4-D+ 0.5 mg/L BA. The highest volume of induced callus was formed on MS medium 0.1 mg/L 2,4-D+ 0.5 mg/L BA using leaf as explant. Root induction from some explants was observed in different treatments. The highest fresh weight of calli belonged to the leaf explant cultured on MS medium containing 1 mg/L 2,4-D+ 0.5 mg/L BA. Callus induction and rooting occurred easily and the explants did not respond well to regeneration.
