S. Anbarestani; A.R. Rezazadeh; A. Rezaei
Abstract
Black cumin (Nigella sativa L.) is a medicinal plant belonging to the Ranunculaceae family. The seeds of Nigella sativa, also known as black seed, are used in traditional medicine as a natural remedy for several illnesses including asthma, inflammation, diabetes, and hypertension. The cell culture of ...
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Black cumin (Nigella sativa L.) is a medicinal plant belonging to the Ranunculaceae family. The seeds of Nigella sativa, also known as black seed, are used in traditional medicine as a natural remedy for several illnesses including asthma, inflammation, diabetes, and hypertension. The cell culture of this plant is important because of its active ingredients and significance in medicine. In this research, the effects of fungal elicitor (zero, 0.5 and 1 mg/L), and sucrose (30, 45 and 60 g/L) on the cell culture of black cumin was investigated as a factorial experiment in a completely randomized design with three replications. The cell growth characteristics and biochemical traits were assayed. The results showed that fungal elicitor and sucrose increased the membrane lipid peroxidation, protein concentration, phenol contents, hydrogen peroxide, and anthocyanin whereas peroxidase activity was significantly decreased compared to control. Generally, sucrose and fungal elicitor increased the growth and activity of the cell and increased the protein production by increasing oxidative stress in cells and increasing the entry of substances into the cell and stimulating metabolism.
A.R. Zarei; S. Zohrabi; F. Boomeh
Abstract
The amount of evapotranspiration or amount of water consumed by plant is the basis of irrigation planning. Irrigation without awareness of the actual plant requirement for water causes economic losses and reduced water use efficiency. One of the best approaches to assess plant water requirement is determination ...
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The amount of evapotranspiration or amount of water consumed by plant is the basis of irrigation planning. Irrigation without awareness of the actual plant requirement for water causes economic losses and reduced water use efficiency. One of the best approaches to assess plant water requirement is determination of crop coefficient using lysimeters. The aim of this study, which was conducted at Fasa University, located 140 kilometers south-east of Shiraz, was to evaluate the different growth stages of Nigella sativa L. and determine the crop coefficient of this annual species. In this study, the crop coefficient of the study species was determined by using a weighing microlysimeter (40×30 cm) and three replications in a good moisture condition. The results showed that the length of each growth stage (initial, developmental, middle and final growth stage) was calculated to be 10, 11, 41 and 23 days, respectively. The crop coefficient (Kc) of Nigella sativa in each stage was calculated to be 0.75, 1.02, 1.21 and 0.8, respectively.
S. Zishan; R. Asghari Zakaria; N. Zare
Abstract
Nigella Sativa L. is an annual plant from Ranunculacea family. Its seeds contain protein, alkaloids, kinons, saponin, and volatile essential oil used as antibacterial agent and treatment of some diseases. This study was aimed to investigate the polyploidy induction in Nigella sativa via seed treatment ...
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Nigella Sativa L. is an annual plant from Ranunculacea family. Its seeds contain protein, alkaloids, kinons, saponin, and volatile essential oil used as antibacterial agent and treatment of some diseases. This study was aimed to investigate the polyploidy induction in Nigella sativa via seed treatment at colchicine concentrations of 0, 0.025%, 0.05%, 0.1%, and 0.2% for 8, 24 and 48 hours. The study was conducted in a factorial experiment based on completely randomized design with three replications. The percentage of tetraploid plants was determined through the morphological and chromosomal studies. Results of analysis of variance showed that effects of colchicine level, treatment duration, and the interaction between them were significant on the survival of plants and percentage of tetraploidy induction. The seed germination and viability of plants significantly decreased with increasing of colchicine concentration and treatment duration. The lowest number of survived plants was observed at a concentration of 2.0% colchicine and a treatment time of 24 and 48 hours. The highest percentage of induced tetraploid plants (9.9%) was obtained at a concentration of 0.05% colchicine with a treatment time of 48 hours, showing no significant difference with concentrations of 1 and 2 % at the same treatment time. The plants treated with colchicine showed higher plant height, internodes length, capsule width and length compared to that of control plants. Furthermore, they had larger stomata with lower number as compared with diploid plants.
R. Kabiri; H. Farahbakhsh; F. Nasibi
Abstract
This research was aimed to evaluate the effect of drought stress, induced by polyethylene glycol (PEG6000), on physiological and biochemical parameters of black cumin (Nigella sativa L.). The traits were included: shoot dry weight, electrolyte leakage, relative water content, photosynthetic pigments ...
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This research was aimed to evaluate the effect of drought stress, induced by polyethylene glycol (PEG6000), on physiological and biochemical parameters of black cumin (Nigella sativa L.). The traits were included: shoot dry weight, electrolyte leakage, relative water content, photosynthetic pigments (chlorophyll a, b, total chlorophyll and carotenoids), malondialdehyde and other aldehyde's contents, anthocyanines, polyphenol compounds, flavonoids, phenylalanine ammonialayse activity, soluble sugar content and protein. An experiment was conducted as a completely randomized design with three replications under hydroponic culture at research laboratory of Shahid Bahonar University of Kerman in 2011. Treatments were four levels of drought stress (0, -0.2, -0.4 and -0.6 MPa). Results showed that drought stress caused a significant reduction in shoot dry weight, relative water content, photosynthetic pigments, anthocyanines, polyphenol compounds, flavonoids and protein and an increase in electrolyte leakage, malondialdehyde content, phenylalanine ammonialayse activity and soluble sugar. Based on the obtained results, it seems that this plant is sensitive to drought stress, and the application of exogenous protective compounds could increase the antioxidant capacity of plant against stress condition.
M.S. Salamati; H. Zeinali
Abstract
This study was performed to investigate genetic diversity and relationships among morphological traits in 21 genotypes of Nigella sativa L., in a completely randomized design with four replications. Morphological traits included seed yield/plant, biological yield, follicle number, seed number per follicle, ...
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This study was performed to investigate genetic diversity and relationships among morphological traits in 21 genotypes of Nigella sativa L., in a completely randomized design with four replications. Morphological traits included seed yield/plant, biological yield, follicle number, seed number per follicle, 1000- seed weight, number of stem branches, follicle weight, plant height and harvest index. Results of analysis of variance showed significant differences for all studied traits (p < 0.01) except harvest index. Phenotypic and genotypic coefficients of variation were high for most traits indicating high diversity of the studied traits. Seed yield varied from 63.34 g in genotype of Shiraz to 147.36 g in genotype of Zabol 1. Correlation coefficients among traits showed that seed yield/plant had a significant and positive correlation with biological yield, seed number per follicle, plant height, number of stem branches and harvest index. Results of stepwise regression analysis for seed yield showed that biological yield, seed number per follicle, number of stem branches and harvest index were entered into the model, respectively, and 95 percent of total variation of seed yield was justified. Path analysis showed that biological yield and seed number per follicle had the highest direct effect on seed yield per plant. Factor analysis revealed three factors that justified 94.12 percent of the total variation among studied traits. These factors were respectively named as efficiency factor (seed yield and biological yield), yield component factor (number of stem branches and follicle number per plant) and follicle factor (follicle weight and seed number per follicle). According to the cluster analysis, 21 genotypes were classified into 3 groups and there were significant differences among the groups especially in terms of seed yield, follicle number per plant and seed number per follicle. Consequently, crossing between superior genotypes of different clusters and testing their progeny through breeding and selection programs may result in production of cultivars with desirable agronomic characteristics.
A.R. Rezapor; M. Heidari; M. Galavi; M. Ramrodi
Abstract
In order to study the effects of water stress and different amounts of sulfur fertilizer on the grain yield, grain yield components, two osmotic components (carbohydrate and proline) and essential oil yield in Nigella sativa L. a field experimental split-plot design with three replications was conducted ...
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In order to study the effects of water stress and different amounts of sulfur fertilizer on the grain yield, grain yield components, two osmotic components (carbohydrate and proline) and essential oil yield in Nigella sativa L. a field experimental split-plot design with three replications was conducted in 2009 at Ghaen city. Treatments included three levels of irrigation as 50, 100 and 150 mm evaporation from class A pan evaporation in main plot and four levels of sulfur fertilizer included 0, 75, 150 and 225 kg ha-1 as Bentonite in sub plot. Results showed that water stress significantly affect the grain yield of Nigella sativa as grain yield decreased up to 22.8% at the level of 150 mm evaporation from the class A evaporation. Except 1000-seed weight, water stress significantly decreased all grain yield components, including biomass production, the number of capsules per plant and seeds per capsule. Maximum reduction at the highest level of water stress related to the number of seed capsules (31.64 percent). Carbohydrate and proline increased under water stress while essential oil yield decreased in Nigella sativa. Sulfur fertilizer improved grain yield as applying 225 kg/ha sulfur fertilizer increased it up to 7.2 percent. Sulfur fertilizer especially at 150 kg/ha had significant positive effects on the grain yield components, osmotic solutes and essential oil yield.
M. Ghorbanli; ْا. Bakhshi Khaniki; S Salimi Elizei; M. Hedayati
Abstract
In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: ...
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In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: 1) Control with irrigation equal to field capacity, 2) two third of field capacity, 3) one third of field capacity, 4) two third of field capacity along with 10 mM ascorbic acid, 5) two third of field capacity along with 1 mM ascorbic acid, 6) one third of field capacity along with 10 mM ascorbic acid, 7) one third of field capacity along with 1 mM ascorbic acid. Proline and soluble sugars levels in shoots and roots were significantly different for all treatments except for the treatment with 2/3 field capacity along with 10 mM ascorbic acid. Enzymes levels in 1/3 and 2/3 treatments were significantly different in both shoots and roots. One mM ascorbic acid along with different field capacity showed no reducing effect. However, 10 mM ascorbic acid especially in 2/3 field capacity could reduce the effect of water deficit through reduction in level of metabolites produced in response to low irrigation.
M. Ghorbanli; N. Adib hashemi; M. Peyvandi
Abstract
In this study, the sodium chloride and ascorbic acid interaction on growth parameters, photosynthetic pigments (chlorophylls a, b and (a+b), carotenoids), amount of soluble sugar and total protein in Nigella sativa L. were investigated in greenhouse condition. This experiment was conducted in randomized ...
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In this study, the sodium chloride and ascorbic acid interaction on growth parameters, photosynthetic pigments (chlorophylls a, b and (a+b), carotenoids), amount of soluble sugar and total protein in Nigella sativa L. were investigated in greenhouse condition. This experiment was conducted in randomized design based on three replications. Plants were exposed to different concentrations of sodium chloride (0, 25, 50, 75 and 100 mM) and ascorbic acid (0 and 10 mM). In plants only exposed to sodium chloride, with the increase of sodium chloride concentration growth parameters, photosynthetic pigments and protein amount decreased compared to control samples while, amount of soluble sugar increased. In plants exposed to sodium chloride and ascorbic acid, growth parameters, photosynthetic pigments, amount of soluble sugars and total protein were higher compared to plants only exposed to sodium chloride. The result showed that spray of ascorbic acid (as an antioxidant) caused resistance against salt stress and decreased side effects of sodium chloride in Nigella sativa L.
S. Asgary; N. Jafari Dinani; A.R. Ghanadi; A.R. Helalat
Volume 24, Issue 1 , May 2008, , Pages 66-73
Abstract
Hypercholesterolaemia is among the most common health problems treated with traditional remedies. Nigella sativa is an effective plant for hypercholesterolemia treatment. However the effect of this herb on hematologic factors and hemostase system has not been elucidated. This study was designed to investigate ...
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Hypercholesterolaemia is among the most common health problems treated with traditional remedies. Nigella sativa is an effective plant for hypercholesterolemia treatment. However the effect of this herb on hematologic factors and hemostase system has not been elucidated. This study was designed to investigate the effect of Nigella sativa on these factors in both normal and hypercholestderolemic rabbits. In this research, twenty rabbits were randomly distributed into four groups of five each. Having normal diet and normal diet + Nigella sativa (5٪), hypercholesterolemic diet (1٪) and hypercholesterolemic diet + Nigella sativa groups. Animal groups received their own diets and water and libitum for a period of 8 weeks. The results indicate that Nigella sativa diet only significantly increase numbers of platelet and do not effect on white and red blood cell, hemoglobin, hematocrit and VII factors. Dietary use of Nigella sativa except increasing platelet number that may be causes increase coagulation don’t have effect on other hematologic factors. Thus these results call for more research on effect of different diet (hypercholesterolemic and normal diet) supplemented with Nigella sativa on different coagulation factors and hemostase system.
