M. Ghorbanli; A. Kiapour
Abstract
Several defense systems are cooperating together in plants to cope with stressful situations. One of these stresses is heavy metals pollution of the plant's growing environment. In This article, the effect of different concentrations of copper was studied on photosynthetic and non-photosynthetic pigments, ...
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Several defense systems are cooperating together in plants to cope with stressful situations. One of these stresses is heavy metals pollution of the plant's growing environment. In This article, the effect of different concentrations of copper was studied on photosynthetic and non-photosynthetic pigments, non-enzymatic and enzymatic defence systems in Portulaca oleracea L. In order to show the effect of so-called heavy metal, copper, on pigments, anthocyanines, flavonoides, phenolic compounds and the activity of antioxidant enzymes some experiments were performed in a completely randomized design and statistical differences were applied at the p ≤ 0.05 level. After planting the plants with the same conditions in the sterile bed, they were nourished with Hoagland's liquid medium. The bed was then amended with graded concentrations (0, 10, 50, 100, 500, 1000 and 1500 µM) of copper salt, in the form of CuSO4.5H2O, separately as treatments and with the control group. Three replicates were maintained for each treatment and also for control groups. After ten days of applying the treatments, the plants were harvested for performing the experiments. After measuring and applying the statistical differences at the 0.05 level via SPSS software, these results were obtained: The amount of chlorophyll a and b was decreased. Amounts of carotenoides, anthocyanines, flavonoides and phenolic compounds were significantly increased. Peroxidase activity was increased and catalase activity was decreased in both shoots and roots.
M. Ghorbanli; ْا. Bakhshi Khaniki; S Salimi Elizei; M. Hedayati
Abstract
In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: ...
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In this research, proline, soluble sugars, catalase and glutathione peroxidase levels which change in response to water deficiency and the effect of ascorbic acid as protecting factor in Nigella sativa L. were surveyed. Plants were cultured in greenhouse and irrigated based on the following treatments: 1) Control with irrigation equal to field capacity, 2) two third of field capacity, 3) one third of field capacity, 4) two third of field capacity along with 10 mM ascorbic acid, 5) two third of field capacity along with 1 mM ascorbic acid, 6) one third of field capacity along with 10 mM ascorbic acid, 7) one third of field capacity along with 1 mM ascorbic acid. Proline and soluble sugars levels in shoots and roots were significantly different for all treatments except for the treatment with 2/3 field capacity along with 10 mM ascorbic acid. Enzymes levels in 1/3 and 2/3 treatments were significantly different in both shoots and roots. One mM ascorbic acid along with different field capacity showed no reducing effect. However, 10 mM ascorbic acid especially in 2/3 field capacity could reduce the effect of water deficit through reduction in level of metabolites produced in response to low irrigation.
M. Ghorbanli; A. Sateei; S. Nasiri Savadkohi
Abstract
Plantago species generally are grassy plants that belong to Plantaginaceae, with large lamina on the ground surfaces. Plantago major L. grows widely in Asia, Europea, Africa, and North American and contains chemical compounds such as polysaccharides, lipids, coffeic acid derivatives, flavonoids, terpenoids, ...
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Plantago species generally are grassy plants that belong to Plantaginaceae, with large lamina on the ground surfaces. Plantago major L. grows widely in Asia, Europea, Africa, and North American and contains chemical compounds such as polysaccharides, lipids, coffeic acid derivatives, flavonoids, terpenoids, ascorbic acid, benzoic acid, ferrolic acid, galactoronic acid and so on. In this study, Plantago major L. plants were cultured in several pots irrigated with hougland solutions and after 20 days, during 3 leaves stage, irrigation was continued with hougland solutions containing different concentrations of CuSO4, 0 (control), 100, 300, 500, 700 and 100µM. Antioxidant enzymes activities (catalase, peroxidase, poly phenol oxidase, ascorbate peroxidase) and phenolics content in leaves and roots were measured. The aim of the study was the effect of copper concentrations on antioxidant enzymes and phenolic compounds content in plantago major L. The result shows that by increasing concentration of copper in the medium, activity of catalse, ascorbate peroxidase and peroxidase in the root and leaves decreased significantly (0.05) in comparison with control. Polyphenol oxidase enzyme activity in roots and leaves increased, that was only significant in roots. Phenolics content increase in roots that was not significant but the decrease in leaves was significant.
M. Ghorbanli; N. Adib hashemi; M. Peyvandi
Abstract
In this study, the sodium chloride and ascorbic acid interaction on growth parameters, photosynthetic pigments (chlorophylls a, b and (a+b), carotenoids), amount of soluble sugar and total protein in Nigella sativa L. were investigated in greenhouse condition. This experiment was conducted in randomized ...
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In this study, the sodium chloride and ascorbic acid interaction on growth parameters, photosynthetic pigments (chlorophylls a, b and (a+b), carotenoids), amount of soluble sugar and total protein in Nigella sativa L. were investigated in greenhouse condition. This experiment was conducted in randomized design based on three replications. Plants were exposed to different concentrations of sodium chloride (0, 25, 50, 75 and 100 mM) and ascorbic acid (0 and 10 mM). In plants only exposed to sodium chloride, with the increase of sodium chloride concentration growth parameters, photosynthetic pigments and protein amount decreased compared to control samples while, amount of soluble sugar increased. In plants exposed to sodium chloride and ascorbic acid, growth parameters, photosynthetic pigments, amount of soluble sugars and total protein were higher compared to plants only exposed to sodium chloride. The result showed that spray of ascorbic acid (as an antioxidant) caused resistance against salt stress and decreased side effects of sodium chloride in Nigella sativa L.
Zh. Asadi Kavan; M. Ghorbanli; A. Sateei
Abstract
Drought stress provokes ROS production in plant cell chloroplasts and subsequently causes lipid membrane peroxidation and damage. Pimpinella anisum L. is one of the aromatic herbal plants which has great export value. The aim of this study was applying exogenous ascorbate in order to control oxidative ...
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Drought stress provokes ROS production in plant cell chloroplasts and subsequently causes lipid membrane peroxidation and damage. Pimpinella anisum L. is one of the aromatic herbal plants which has great export value. The aim of this study was applying exogenous ascorbate in order to control oxidative stress during drought tolerance. Changes of pigment content of leaves, total phenol compounds, malonedialdehyde (MDA) content were measured. In a pot study, drought stress introduced to treatments with 3 replicates based on 3 levels of field capacity (100, 60 and 25%) and ascorbate (1.4 mM) sprayed on them. Chlorophyll content and chlorophyll a/b ratio decreased with increasing in stress levels, while flavonoids and anthocyanins increased. Carotene and xanthophyll increased only in moderate stress level due to drought. Exogenous ascorbate increased chlorophylls and carotenoid content but decreased flavonoid and anthocyanin contents and had great effect on increasing phenol compound in all stress levels. MDA content remained relatively constant, but increased significantly in severe stress levels. Applying exogenous ascorbate led to decreasing metabolite. According to the results exogenous ascorbate could increase the ability of Pimpinella anisum in response to drought stress with different mechanisms and had protective effect against lipid peroxidation due to drought stress.
