Document Type : Research Paper
Authors
1 Ph.D. student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran
3 Department of Horticulture Science and Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran
Abstract
Seed pretreatment with the cold plasma and salicylic acid is one of the methods to improve the seed germination and quantitative and qualitative growth of plants. Therefore, an experiment was conducted to investigate the effects of seed pretreatment with the cold plasma (0 and 100 W for 4 min) and seed pretreatment and foliar application of salicylic acid in the micro and nano forms (0 and 1.5 mM salicylic acid) to improve the growth and production of secondary metabolites in Salvia leriifolia Benth. The results showed that the use of salicylic acid as seed pretreatment and foliar application increased the plant height, chlorophylls a and b, total phenol, and caffeic acid of the leaves significantly. Also, the seed pretreatment with nano salicylic acid form and foliar application of micro salicylic acid form and cold plasma increased the seedlings fresh and dry weight and phenylalanine ammonialyase and tyrosine ammonialyase enzymes by 3.48, 13.3, 227.7, and 277%, respectively compared to the control. The seed pretreatment and application of salicylic acid did not have a positive effect on increasing the amount of rosmarinic and salvianolic acids of the leaves compared to the control. According to the results, increasing the enzymes activity involved in the biosynthesis of phenolic acids could affect the amount of caffeic acid positively. Overall, the findings of the present study showed that the seed pretreatment with salicylic acid and foliar application of nano salicylic acid could improve the growth traits and activity of enzymes involved in the biosynthesis of secondary metabolites in S. leriifolia more than other treatments.
Keywords
Main Subjects
- Adhikari, B., Adhikari, M. and Park, G., 2020. The effects of plasma on plant growth, development and sustainability. Applied Sciences, 10(17): 6045.
- Akkol, E.K., Goger, F., Kosar, M. and Baser, K.H., 2008. Phenolic composion and biological activities of Salvia halophila and Salvia virgate from Turkey. Food Chemistry, 108: 942-949.
- Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23: 112-121.
- Arvin, M.J., Bidmeshki, A., Keramat, B. and Maghsoudi, K., 2011. The role of salicylic acid in reducing the effects of drought stress on morphological and physiological parameters in garlic. 7th Iranian Congress of Horticultural Sciences, Isfahan, 14-17 September: 942-943.
- Asnavandi, A., Barzin, G., Davari Mahabadi, T., Entezari, M. and Pishkar, L., 2021. Study of anatomical changes of fennel plants following cold plasma radiation. Journal of Cell & Tissue (JCT), 12(1): 11-19.
- Başkan, S., Öztekin, N. and Erim, F.B., 2007. Determination of carnosic acid and rosmarinic acid in sage by capillary electrophoresis. Food Chemistry, 101(4): 1748-1752.
- Beaudoin-Eagan, L.D. and Thorpe, T.A., 1985. Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiology, 78: 438-441.
- Dehghan Niri, F., Saffari, V.R. and Moghsoudi Moud, A.A., 2016. Effect of salicylic acid on photosynthetic pigments and chlorophyll flurescence of pot marigold under salt stress conditions. Iranain Journal of Horticultur and Technology, 17(1): 77-88.
- Dokhanieh, A.Y., Aghdam, M.S., Fard, J.R and Hassanpour, H., 2013. Postharvest salicylic acid treatment enhances antioxidant potential of cornelian cherry fruit. Scientia Horticulturae, 154: 31-36.
- Dong, C.J., Wang, X.L. and Shang, Q.M., 2011. Metabolism that confers tolerance to salinity stress in cucumber seedlings. Scientia Horticulturae, 129: 629-636.
- Dong, J., Wan, G. and Liang, Z., 2010. Accumulation of salicylic acid-induced phenolic compounds and raised activities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell culture. Journal of Biotechnology, 148: 99-104.
- Ebrahimabadi, A.H., Mazoochi, A., JookarKashi, F., Djafari-Bidgoli, Z. and Batooli, H., 2010, Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophila Boiss. from Iran. Food and Chemical Toxicology, 48: 1371-1376.
- Ejtahed, R.S., Radjabian, T. and Hoseini tafreshi, S.A., 2015. Ammonia lyase gene and rosmarinic acid production in Salvia officinalis and Salvia virgata shoots uunder salicylic acid elicitation. Applied Biochemistry and Biotechnology, 175: 1846-1858.
- Fadhlalmawla, S.A., Mohamed, A.A.H., Almarashi, J.Q. and Boutraa, T., 2019. The impact of cold atmospheric pressure plasma jet on seed germination and seedlings growth of fenugreek (Trigonella foenum-graecum). Plasma Science Technology, 21(10): 105503.
- Farhadi, N., Estaji, A. and Alizadeh Salteh, S., 2016. The Effect of pretreatment of salicylic acid on seed germination of milk thistle (Silybum marianum cv. Budakalaszi) under salinity and drought stress. Iranian Journal of Seed Research, 3(1): 75-84.
- Farsaraei, S., Moghaddam, M. and Mehdizadeh, L., 2019. Effect of salicylic acid on growth and biochemical characteristics and essential oil percentage of Lemon verbena at different irrigation water salinity. Journal of Water Research in Agriculture (Soil and Water Science), 33(1): 95-108.
- Fereyduni, M., 2016. Microscopic investigation of cold plasma effect on chickpea seed germination. 3rd International Conference on Applied Research in Agricultural Sciences, Tehran, 20 February.
- Fotovvat, M., Radjabian, T., Saboora, A., Ranjbar, M. and Sadat Ejtahed, R.S., 2014. Comparative analysis of rosmarinic acid and salvianolic acids A and B contents in four wild growing Salvia species from Iran. Iranian Journal of Biology, 27(5): 936-937.
- Ghai, N., Setia, R.C. and Setia, N., 2002. Effect of paclobutrazol and salicylic acid on chlorophyll content, hill activity and yield components in Brescia napus L. (cv. GSL-1). Phytomorphology, 52: 83-87.
- Ghasimi Hagh, Z., Jokar, S., Bodaghi, H. and Modarres, M., 2018. Effect of salicylic acid and methyl jasmonate on the production of rosmarinic acid and caffeic acid in callus culture of Salvia lerrifolia Benth. Iranian Journal of Plant Biology, 10(35): 68-80.
- Hamada, A.M. and Al-Hakimi, A.M.A., 2001. Salicylic acid versus salinity drought-induced stress on wheat seedlings. Rostlinna Vyroba, 47(10): 444-450.
- Hedge, I.C., 1986. Labiatae In: Flora Iranica (Ed. Rechinger, K.H.). vol. 150. Akademische Druck-U Verlagsanstalt, Graz.
- Hoagland, R.E. and Duke, S.O., 1981. Effect of herbicides on extractable phenylalanine ammonia lyase activity in light- and dark-grown soybean (Glycine max) seedlings. Weed Science, 29(4): 433-439.
- Hosseinzadeh, H., Sadeghnia, H.R., Imenshahidi, M. and Fazly Bazzaz, B.S., 2009. Review of the pharmacological and toxicological effects of Salvia leriifolia. Iranian Journal of Basic Medical Sciences, 12(1): 1-8.
- Ivankov, A., Nauciene, Z., Degutyt eFomins, L., Žukiene, R., Januškaitiene, I., Malakauskiene, A., Jakštas, V., Ivanauskas, L., Romanovskaja, D., Šlepetiene, A., Filatova, I., Lyushkevich, V. and Mildažiene, V., 2021. Changes in agricultural performance of common buckwheat induced by seed treatment with cold plasma and electromagnetic field. Applied Sciences, 11(10): 4391.
- Jiang, J., Lu, Y., Li, J., Li, L. and He, X., 2014. Effect of seed treatment by cold plasma on the resistance of tomato to ralstonia solanacearum (Bacterial Wilt), PLoS ONE, 9(5): e97753.
- Jiayun, T., Rui, H., Xiaoli, Z. and Ruoting, Z., 2014. Effects of atmospheric pressure air plasma pretreatment on the seed germination and early growth of Andrographis paniculata. Plasma Science and Technology, 16(3): 260.
- Kabiri, R. and Naghizadeh, M., 2016. Study the effect of salicylic acid pretreatment on germination and early growth of black cumin (Nigella sativa) under salinity stress. Iranian Journal of Seed Science and Technology, 4(1): 61-72.
- Kahkonen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S. and Heinonen, M., 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47(10): 3954-3962.
- Kamatou, G.P.P., Makunga, N.P., Ramogola, W.P.N. and Viljoen, A.M., 2008. South African Salvia species: a review of biological activities and phytochemistry. Journal of ethnopharmacology, 119: 664-672.
- Kim, N.S., Jung, D.H., Jung, C.R., Jeon, K.S., Park, H.W. and Park, S.U., 2020. Improvement of phenylpropanoid production with elicitor treatments in Pimpinella brachycarpa Nakai. Horticulturae, 6(4): 108.
- Kumar, R., Thakur, A., Vikram, A., Vaid, A. and Rane, R., 2019. Effect of cold plasma treatment of seeds on quality of seed crop of okra. International Journal of Economic Plants, 6(2): 073-077.
- Lakzayi, M., Sabbagh, E., Rigi, K. and Keshtehgar, A., 2014. Effect of salicylic acid on activities of antioxidant enzymes, flowering and fruit yield and the role on reduce of drought stress. International Journal of Farming and Allied Sciences, 3: 980-987.
- Ling, L., Jiafeng, J., Jiangang, L., Minchong, S., Xin, H., Hanliang, S. and Yuanhua, D., 2014. Effects of cold plasma treatment on seed germination and seedling growth of soybean. Nature, 4: 5859.
- Lu, Y. and Foo, L.Y., 2002. Polyphenolics of Salvia-a review. Phytochemistry, 59: 117-140.
- Maleki, M.S. and Ehsanpour, A., 2018. Effect of salicylic acid on total phenol, flavonoid, anthocyanin and PAL and TAL enzymes in tomato (Solanum lycopersicum Mill.) plants. Iranian Journal of Plant Biology, 9(4): 55-67.
- Milda Žiene, V., Pauzaite, G., Nauciené, Z., Malakauskiene, A., Zukiene, R. and Januskaitiene, I., 2018. Pre-sowing seed treatment with cold plasma and electromagnetic field increases secondary metabolite content in purple coneflower (Echinacea purpurea) leaves. Plasma Processes and Polymers, 15: 1700059.
- Min-Hui, L., Jian-Min, C., Yong, P. and Pei-Gen, X., 2008. Distribution of phenolic acids in Chinese Salvia plants. World Science and Technology, 10(5): 46-52.
- Miura, K. and Tada, Y., 2014. Regulation of water, salinity, and cold stress responses by salicylic acid. Frontiers in Plant Science, 5: 410.
- Modarres, M., 2007. Investigation about phenology physiological and biochemical characteristics of Saliva leriifolia. Thesis, Biology Department, Ferdowsi University of Mashhad, Iran.
- Moghanloo, M., Iranbakhsh, A., Ebadi, M. and Oraghi Ardebili, Z., 2019. Differential physiology and expression of phenylalanine ammonia lyase (PAL) and universal stress protein (USP) in the endangered species Astragalus fridae following seed priming with cold plasma and manipulation of culture medium with silica nanoparticles. 3 Biotech, 9(7): 288.
- Mohammadi, S.A., Khorram, S. and Haji Baba, A., 2016. Study the effect of different low temperature plasmas on growth of wheat seed. Research Institute of Applied Physics and Astronomy, 24-26 August: 101-105.
- Mozaffarian, V., 1996. Dictionary of Iranian plant names. Farhang Moaser, Tehran, 477p.
- Namdeo, A.G., 2007. Plant cell elicitation for production of secondary metabolites: a review. Pharmacognosy Reviews, 1(1): 69-79.
- Navarro, E., Baun, A., Behra, R., Hartmann, N.B., Filser, J., Miao, A.J. and Sigg, L., 2008. Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants, and fungi. Ecotoxicology, 17(5): 372-386.
- Nemati Mirak, Y., Khademi, O., Rohollahi, I., Bostani, A. and Karimi, N., 2019. Comparison on the effects of salicylic acid and nano-salicylic acid in reducing the effects of salinity stress on ‘Camarosa’ strawberry fruit. Pomology Research, 3(2): 79-93.
- Roshdy, A.E., Alebidi, A., Almutairi, K., Al-Obeed, R. and Elsabagh, A., 2021. The effect of salicylic acid on the performances of salt stressed strawberry plants, enzymes activity, and salt tolerance index. Agronomy, 11(4): 775.
- Saberi, M., Modarres-Sanavy, S.A.M., Zare, R. and Ghomi, H., 2018. Amelioration of photosynthesis and quality of wheat under nonthermal radio frequency plasma treatment. Scientific Reports, 8: 11655.
- Sauvesty, A., Page, F. and Huot, J., 1992. A simple method for extracting plant phenolic compounds. Canadian Journal of Forest Research, 22: 654-659.
- Shabani, L. and Ehsanpour, A.A., 2010. Induction of antioxidant enzymes, phenolic and flavonoid compounds in in vitro culture of licorice (Glycyrrhiza glabra L.) using methyl jasmonate and salicylic acid. Iranian Journal of biology, 22(4):
691-703.
691-703.
- Shadkam, S. and Karimi, J., 2019. Effect of cold plasma on seed germination. National Conference on Industry and Commercialization Agricultural, Ahvaz, 19 December.
- Shakirova, F.M. and Sahabutdinova, D.R., 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164 (3): 317-322.
- Shoghian, M. and Rozbahani, A., 2018. The effect of salicylic acid foliar application on morphological traits, yield and yield components of red bean under drought tension conditions. Crop Physiology Journal, 9(34): 131-147.
- Singh, A. and Dwivedi, P., 2018. Methyljasmonate and salicylic acid as potent elicitors for secondary metabolite production in medicinal plants: A review. Journal of Pharmacognosy and Phytochemistry, 7(1): 750-757.
- Song, X., GuoYong, H. and Chang, L.X., 2020. Effects of salicylic acid and sucrose on pigment content in Pistacia chinensis leaves. Scientia Horticulturae, 259: 108783
- Tajbakhsh, M., Hasanzadeh, A. and Aghaii, R., 2014. Effect of different treatments. Agronomy Journal (Pajouhesh & Sazandegi), 28(4): 74-84.
- Tucuch-Haas, C., Alcántar-González, G., Trejo-Téllez, L., Volke-Haller, H., Salinas-Moreno, Y. and Larqué-Saavedra, A., 2017. Effect of salicylic acid on growth, nutritional status and performance of maize (Zea mays). Agrociencia, 51: 771-781.
- Verma, V., Ravindran, P. and Kumar, P.P., 2016. Plant hormone-mediated regulation of stress responses. BMC Plant Biology, 16: 86.
- Wang, H.F., Provan, K. and, Helliwell, K., 2004. Determination of rosmarinic acid and caffeic acid in aromatic herbs by HPLC. Food Chemistry, 87: 307-311.
- Wang, L.J., Fan, L., Loesche, W., Duan, W., Lie, G.J., Cheng, J.S., Lou, H.B. and Li, S.H., 2010. Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. BMC Plant Biology, 10: 34.
- War, A.R., Paulraj, M.G., War, M.Y. and Ignacimuthu, S., 2011. Jasmonic acid-mediated-induced resistance in groundnut (Arachis hypogaea L.) against Helicoverpa armigera. Journal of Plant Growth Regulation, 30(4): 512-523.
- Wen, P.F., Chen, J.Y., Kong, W.F., Pan, Q.H., Wan, S.B. and Huang, W.D., 2005. Salicylic acid induced the expression of phenylalanine ammonia-lyase gene in grape berry. Plant Science, 169(5): 928-934.
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