A.A Hossienpour Ggazviniy; M.A. Alizadeh; A.A. Jafari; A.R. Valadabadi
Abstract
In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised ...
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In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised design with three replications were used. The different treatments including: cold stratification, after-ripening, physical scarification (sand paper), chemical scarification (Ethylic alcohol 70%) and control were used for dormancy breaking. The treated seeds were sown in 3 petri dishes as replications. Then, all samples were placed in a germinator with condition of 20°C and light-to-dark cycle of 16 hours light (1000 lux) to 8 hours dark. The germination characteristics including: germination percentage, speed of germination, length of root and shoot, vigour index, fresh weight and dry weight were evaluated during 18 days of the experiment. There were significant differences amoung species for all seed germination characteristics. The ecotype of Khuzistan from S. khuzistanica had more seed germination and vigour compared to the other three species. The seed characteristics specially speed of germination and vigour index of ecotypes from cold zone were affected by cold treatment more than the other treatments.
F.S. Serry; A. Ghamari-Zare; Sh. Shahrzaad; M.A. NaderiShahab; S. Kalate-jary
Abstract
Salvia leriifolia Benth. (Noruozak) is a perennial plant which belongs to the Lamiaceae family. This herb has a great importance in the sense of pharmaceutical applications. In the nature, seeds of Salvia leriifolia hardly germinate endangering its survival. In this work, in addition of studying the ...
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Salvia leriifolia Benth. (Noruozak) is a perennial plant which belongs to the Lamiaceae family. This herb has a great importance in the sense of pharmaceutical applications. In the nature, seeds of Salvia leriifolia hardly germinate endangering its survival. In this work, in addition of studying the causes of low germination rate, different physic-chemical treatments such as scratching on seed coat, scratch seeds under running water, intact seeds under running water, coatless seeds, treating intact seed with 98% sulphuric acid for 10 and 15 minutes were investigated. Seeds were collected from Abkhandari station in Sabzevar city in 2009. For sterilization, the solution of HgCl2 0.1% w/v was applied for seeds in all of treatments. Also four temperature treatments of 4, 20, 25C° and keeping at 4C° for a week then transferring to 25C° on germination of seeds were considered. There was no seed germination happened in control, mechanical scratching, running water and sulphuric acid treatments. The coatless seeds treatment and incubation at 4C° for a week and then transferring to 25C° has the most effect on seeds germination, with 100% seed germination. According to the results, removing the hard and impermeable seed coat of Salvia leriifolia as a physical prevention and low temperature as a physiological factor were identified as effective method in germination of the mentioned species.
M. Nabaei; P. Roshandel; A. Mohammadkhani
Abstract
Rheum ribes is a plant species in Polygonaceae family which grows in Iran and is considered as a valuable medicinal species in traditional medicine. Rheum ribes seeds hardly germinate because of seed dormancy and may cause difficulties for its massive industrial cultivations. This study was performed ...
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Rheum ribes is a plant species in Polygonaceae family which grows in Iran and is considered as a valuable medicinal species in traditional medicine. Rheum ribes seeds hardly germinate because of seed dormancy and may cause difficulties for its massive industrial cultivations. This study was performed to determine the best treatment for seed dormancy breaking. To achieve this goal, several treatments were applied comprising: Hot water (70 and 90 ˚C) for 15 minutes, moist chilling (at 2 ˚C) for 5, 10, 15, 20 and 25 days, sulfuric acid 75% (5 and 15 minutes), KNO3 (0.2%), gibberellic acid, kinetin, auxin (100, 250, 500 and 1000 ppm) and 24-epibrassinolid (0.5 and 1 ppm), for 24 and 48 h washing (for 24 and 48 h), and finally a combined treatment of gibberellic acid (500 ppm) and chilling at 2 ˚C for 5, 10, 15, 20 and 25 days. According to the results, seed dormancy in Rheum ribes is physiological since the highest germination percentage (96%) was obtained by using the combined treatment of gibberellic acid (500 ppm) and pre-chilling (for 25 days). In addition, the effect of moist chilling treatment –by itself- on breaking seed dormancy was remarkable in this plant as germination increased up to 89%. Effects of hormones were also statistically significant on increasing seed germination but compared with the salient effect of combined chilling and gibberellic acid treatment was not so remarkable. On the other hand, other applied treatments had no effects on breaking seed dormancy in Rheum ribes which indicates that the type of seed dormancy in Rheum ribes is not physical or due to the accumulation of inhibitory substance in seed coat.
T. Rajabian; A. Saboora; B. Hassani; H. Fallah Hosseini
Volume 23, Issue 3 , November 2007, , Pages 391-404
Abstract
Ferula assa-foetida L. is an Iranian endemic medicinal plant that belongs to Apiaceae family. Seeds of this plant have a long period of dormancy. Therefore, experimental methods, which decrease seed dormancy period, could be effective in the seed germination rate and also in revival of the plant. The ...
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Ferula assa-foetida L. is an Iranian endemic medicinal plant that belongs to Apiaceae family. Seeds of this plant have a long period of dormancy. Therefore, experimental methods, which decrease seed dormancy period, could be effective in the seed germination rate and also in revival of the plant. The effect of GA3 as a pre-treatment (at high concentrations in a short time period at 23±2°C) and treatment (low concentrations in a long time period at 23±2 and 4°C)on germination of two Tabas and Shirkooh population seeds on MS medium was analyzed. Comparative analyses on treated and pre- treated seeds from two populations with GA3 at 23±2°C temperature did not show any significant changes in both the germination percentage and rate. However, seeds chilling treatment (4°C) indicated increased germination rate and percentage. So, after 12 weeks the maximum germination percentages were 84 and 56% for Tabas and Shirkooh populations. Also the effect of the temperature was investigated on seed germination on the wet filter paper in two levels of 23±2 and 4°C. According to results, maximum seed germination percentage obtained for Shirkooh were (90%) and Tabas (67%) populations by soaking the seeds on the wet filter papers in Petri dishes within 8 to 9 weeks. Finally, it seems that increasing endogenous GA3 concentration, which is provided mostly by chilling treatment, is the most effective factor for breaking the seed dormancy.
A. Rahmanpour; A. Majd; F. Chalabiane
Abstract
The ripening seeds of Eremurus stenophyllus were collected from existed species from National Botanical Garden of Iran and put under physical stimulator pretreatments including soaking of seeds in water for 24-48h, cutting of seeds top, making abrasion on seed cortex, exposing to light for 24h and 12h ...
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The ripening seeds of Eremurus stenophyllus were collected from existed species from National Botanical Garden of Iran and put under physical stimulator pretreatments including soaking of seeds in water for 24-48h, cutting of seeds top, making abrasion on seed cortex, exposing to light for 24h and 12h and absolute darkness and chemical stimulators like sodium Hypochloride, Citric acid and Gibberellic acid in different concentrations for finding the suitable methods for inducing germination and breaking dormancy by these methods, the maximum percentage of seed germination and the most suitable treatment has been specified. The results showed that the most suitable pretreatment and treatment were soaking of seeds in the water for 24-48h, removing of seed cortex and washing in sodium hypocholoride 35% and distilled water, cutting of seed top and Gibberrelic acid treatment (0.005 M.) for 45 minutes under 10-15°C white light, 24h (4500-5000 lux ) for 1-3 weeks which made the germination percent 75%, germination speed 0.81, seed vigor 6.75 . Treatment with Citric acid (30mg/lit) included germination percent 100%, germination speed 0.86 and seed vigor 9. The results showed some important differences in comparison with controls.
