Agriculture and horticulture
Farhad Bagheri fard sharabiani; Esmaeil Chamani; Mousa Torabi Giglou; Alireza Ghanbari; Younes Pourbeyrami hir
Abstract
Background and objectives: The beneficial effects of the chamomile plant have been attributed to its essential oil, so any factor that affects the quantity of its essential oil will be of interest to researchers. To investigate the effect of plant growth regulators salicylic ...
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Background and objectives: The beneficial effects of the chamomile plant have been attributed to its essential oil, so any factor that affects the quantity of its essential oil will be of interest to researchers. To investigate the effect of plant growth regulators salicylic acid and methyl jasmonate on the production of secondary metabolites in chamomile plants, this experiment was conducted under the native in vitro conditions of Sharbian City.Methodology: To conduct this research, chamomile seeds were collected from Sharbian (N ʺ52 58 37 and E ʺ06 ʹ11 ˚), East Azerbaijan province. After transfer, the seeds were sterilized for 15 minutes in a detergent. Their surfaces were disinfected with 70% alcohol for 45 seconds and then with sodium hypochlorite for 30 minutes. After surface disinfection, they were washed twice with distilled water. The basic culture medium in this study was the MS culture medium. All cultures were placed in the growth chamber at a temperature of 24±2°C and 16 hours of light and 8 hours of darkness. A factorial experiment was performed in a complete random design with three replications. Test treatments included salicylic acid (SA) and methyl jasmonate (MeJA) at five levels (0, 50, 100, 200, 400 μM). Minguez-Mosquera and Perez-Galvez methods were applied to determine photosynthetic pigment amounts. The amount of phenolic compounds in the plant extract was measured with a slight modification based on the Slinkard and Singleton method with the Folin-Ciocalteau reagent. The data related to this research were analyzed using SAS V.9.g.1 statistical software. The comparison of treatment averages was done with Duncan's test at 5% and 1%. Graphs were drawn using Microsoft Excel software.Results: The analysis of variance showed that the interaction effect of the applied treatments on the traits of stem weight, root weight, stem length, and root length is significant at the 1% probability level. In addition, it is significant on the number of stems at the 5% probability level. The comparison of the averages showed that the highest amount of stem weight was obtained in the interaction effect of (50 µM) SA and (200 µM) MeJA, and the lowest amount of stem weight was obtained in the interaction effect of (400 µM) SA and (400 µM) MeJA treatment. The comparison of the means shows a significant difference in this trait. The highest amount of root weight was obtained in the interaction effect of (100 µM) SA and (100 µM) MeJA, and the lowest amount of root weight was obtained in the interaction of (400 µM) SA and (400 µM) MeJA treatment. Came. A comparison of the averages showed that the highest number of stems was obtained in the interaction of (100 µM) SA and (50 µM) MeJA, and the lowest number of stems was obtained in the interaction of (400 µM) SA and (200 µM) MeJA. The comparison of the means showed the significance of the stem and root length traits. The highest value of these two traits was due to the interaction of (50 µM) SA and (100 µM) MeJA treatments, and the lowest value of stem length was in the combination of (400 µM) SA and (µM) treatments. 400) MeJA and root length were measured from the combination of SA (400 µM) and MeJA (200 µM) treatments. In the biochemical traits, variance analysis of the data showed that the effect of SA levels on the amount of chlorophyll a, b, carotenoid, total chlorophyll, phenol, and flavonoid in all three wavelengths was significant at the 1% probability level.Conclusion: In this experiment, it was shown that metabolite production depends not only on enzyme activity but also on stimulant concentration. Increasing the stimulus more than usual not only increases metabolism but also reduces or stops the production of metabolites through the reduction of enzyme activity (probably through the reduction of the corresponding gene expression).
M.A. Alizadeh; H. Khamushi; A.A. Jafari; J. Daneshian
Abstract
Seeds of eight populations of Anthemis pseudocotula Boiss., A. tinctoria L. and A. triumfettiiL. were cultivated as single plant by using three replicated split plot design for planting date as main factor and the populations of three species as minor factor on the basis of randomized block design on ...
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Seeds of eight populations of Anthemis pseudocotula Boiss., A. tinctoria L. and A. triumfettiiL. were cultivated as single plant by using three replicated split plot design for planting date as main factor and the populations of three species as minor factor on the basis of randomized block design on two dates (May, and July). The study was conducted at Alborz Research Station in 2011. Each experimental unit included six rows with a distance of 50 cm and 10 plants per row, with a distance of 40 cm. During the growth period, the traits of crown length and width, plant height, number of flowers, fresh and dry weight were evaluated for the populations of three species. Analysis of variance showed that there were significant differences between two dates of cultivation (p<0.05), as well as among populations and their interaction effects (p<0.01) for all traits. Mean comparison of traits showed that crown length and width, plant height, number of flowers, fresh and dry weight of Anthemis pseudocotula were higher as compared to Anthemistinctoria and A. triumfettii. According to the obtained results, the vegetative and generative traits of Anthemis pseudocotula from 20172-Golestan population were superior as compared to other populations. However, no significant differences were found for the populations of Anthemis tinctoria. Our results clearly showed that Anthemis pseudocotula had lower GDD as compared to other species and flowered earlier. The percentage of essential oil ofAnthemis tinctoria was higher thanthat ofAnthemis pseudocotulaandAnthemistriumfettii.
M. Azizi; M. Rahmati; T. Ebadi; M. Hasanzadeh khayyat
Abstract
Field experiment was carried out at the Ferdowsi University Research Field, Mashhad, Iran during 2007-2008, to determine the effect of different drying temperatures, microwave powers and natural method (shaded and sunny area) in Chamomile (Matricaria recutita L., Asteraceae) essential oil content, chamazolene ...
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Field experiment was carried out at the Ferdowsi University Research Field, Mashhad, Iran during 2007-2008, to determine the effect of different drying temperatures, microwave powers and natural method (shaded and sunny area) in Chamomile (Matricaria recutita L., Asteraceae) essential oil content, chamazolene content, drying time and drying rate. The experimental design was completely randomized block design having three temperatures: 50, 60 and 70 °C, six microwave powers: 100, 180, 300, 450, 600 and 900w and drying in shaded and sunny area, replicated thrice. The drying process was continued until the mass of the sample reduced to a moisture content of about 0.10 on a dry basis or 10% on a wet basis. The results indicate that different treatments of drying had a significant effect on the drying time and rate and essential oil and chamazolene content. The maximum essential oil content obtained at drying by the lowest temperatures and drying in shaded area. Whereas, higher drying temperatures of oven and microwave powers and drying in sunny area decreased the essential oil content. Maximum chamazolene content obtained at microwave drying (except 100w power) and natural method. Minimum chamazolene content was obtained at drying by oven.
S.F. Afzali; H. Shariatmadari; M.A. Hajabbasi; F. Moatar
Volume 23, Issue 3 , November 2007, , Pages 382-390
Abstract
Flavonoids are polyphenolic compounds with strong antioxidant effects. Chamomile (Matricaria chamomilla L.) is used for a variety of herbal remedies, containing classes of biologically active compounds including flavonoids and essential oil. There have also been many reports, showing that flavonol-O-glycosides ...
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Flavonoids are polyphenolic compounds with strong antioxidant effects. Chamomile (Matricaria chamomilla L.) is used for a variety of herbal remedies, containing classes of biologically active compounds including flavonoids and essential oil. There have also been many reports, showing that flavonol-O-glycosides are quantitatively the most abundant flavonoids found in chamomile flowers. Tolerance of this plant on saline and drought conditions has not been well studied and documented. Therefore a greenhouse experiment was conducted using complete randomized design with four replications in order to evaluate the effects of salinity and drought stress on flower dry weight and falavonoids component of chamomile. Hydroponic solutions are used for salinity experiment at five levels of NaCl concentrations (0, 40, 80, 120, 190 mM). In order to examine drought stress, we used gravitational method and four treatments (FC soil moisture content, and 75, 90, 95 depletion percent of soil AWC) in a pot experiment. Then the flower dry weight and their flavonol-O-glycosides were measured. The Results showed that both salinity and drought reduced flower dry weight and flavonoids concentration, but drought reduction effects were more than salinity. Flowers dry weight significantly increased with increasing NaCl concentration up to 40 mM while decreased at higher salinity levels. There were no significant differences between salinity treatments up to 80 mM NaCl for flavonol-O-glycosides concentration. Regarding the dry flower yield, the highest content of flavonol-O-glycosides was observed in 40 mM NaCl treatments. Flower dry weight and Flavonol- O- glycosides decreased as drought levels increased.
M.A. Alizadeh; H.R. Isvand
Volume 20, Issue 3 , October 2004, , Pages 301-307
Abstract
The majority of the crop seeds have physiological dormancy after harvesting. This phenomenon is depending to different physiological dormancy. This phenomenon can overcome by pre-treatment including prechilling, pre-heating, dry storage condition, using chemical for promoting germination and overcoming ...
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The majority of the crop seeds have physiological dormancy after harvesting. This phenomenon is depending to different physiological dormancy. This phenomenon can overcome by pre-treatment including prechilling, pre-heating, dry storage condition, using chemical for promoting germination and overcoming of inhibitor substances. In this study the seeds of two medicinal species (Eruca sativa Lam. and Anthemis altissima L.) under two cold room (4°C) and dry storage (room temperature) conditions were maintained over six months and tested by standard germination test. The physiological quality of the seed rocket plant had not any significance differences in two conditions. The reason might be due to non-physiological dormancy of the seed species. Whereas, percent of germination in the species of chamomile was significant in the two conditions. As it is clear the percent of germination of the chamomile from dry storage condition was higher from cold room condition and this due to physiological dormancy of plant seed which overcome by dry storage condition. The speed and vigor index of plant seed had no significant differences in two conditions.
P. Owlia; H. Saderi; H. Semiyari; A.S. Hosseini; M. Naseri
Volume 19, Issue 1 , May 2003, , Pages 87-99
Abstract
Porphyromonas gingivalis is an obligate anaerobic bacterium. This bacterium is one of the most important agents causing adult periodontitis. Periodontitis is a common oral infection. Oral infections are usually controlled by mouthwashes, for example chlorhexidine and herbal mouthwash. The purpose of ...
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Porphyromonas gingivalis is an obligate anaerobic bacterium. This bacterium is one of the most important agents causing adult periodontitis. Periodontitis is a common oral infection. Oral infections are usually controlled by mouthwashes, for example chlorhexidine and herbal mouthwash. The purpose of the present study was to evalue of antimicrobial effects of mouthwash of Chamomile, essential oil of Chamomile and chlorhexidine. We evaluated the antimicrobial effects by agar diffusion method on supplemented Brucella agar. The growth inhibition zones were measured and compared with each other. Inhibition zone for Chamomile mouthwash, chlorhexidine (0.2%) and essential oil(1:5 dilution) of Chamomile were 13, 29.33 and 19.3 mm, respectively. The results showed that Chamomile have antimicrobial effect on Porphyromonas gingivalis. It appears that we can use Chamomile as mouthwash for treatment and prophylaxis of periodontitis.
