P. Sarkheil; M. Omidi; S.A. Peyghambari; S. Davazdahemami
Abstract
Seeds were cultured on Whatman paper by sterile water in cube, solid MS and solid MS. Seeds were not germinated on medium but 80% of seeds were germinated on Whatman paper, so this method is used as a basic method. Seeds were germinated after four days and after two weeks of culture they had normal roots, ...
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Seeds were cultured on Whatman paper by sterile water in cube, solid MS and solid MS. Seeds were not germinated on medium but 80% of seeds were germinated on Whatman paper, so this method is used as a basic method. Seeds were germinated after four days and after two weeks of culture they had normal roots, shoots and leaves. Cultures were incubated at 25◦ ±2◦C and exposed to 16 hours light per day. Explants were cultured on MS medium supplemented with 3% sucrose and solidified with 0.8% (w/v) agar. The pH of the medium was adjusted to 5.8 before autoclaving. Different types of explants were used for this experiment; root, crown, apical meristem, hypocotyls and leaf. Between different kinds of explants leaf didn’t response to callogenesis. The effects of different combinations of 2,4-D (2,4-Diclorophenoxy acetic acid) and BAP (6-Benzylaminopurine) were studied. Subculture was done every 3 weeks. In order to determine regeneration ability, the initiated callus were transferred to a regeneration medium which was composed of macronutrient, micronutrient and organic components of MS, 2,4-D (0.2 mgL-1), BAP (0.5, 2, 4, 15, 20 and 25 mgL-1) and MS without hormones, 0/3% sucrose, pH 5.8 for 4 weeks. In the presence of 2,4-D (2 and 4 mgL-1) and BAP (0.25 and 0.5 mgL-1) in the callus induction medium, high callus production percentage was reported. The hypocotyls, in contrast to the primary leaf explants, and apical meristem segments were more responsive to the tested combinations of 2,4-D and BAP. The callus from all explants was soft, watery and loose friable. During subculture period, hypocotyls and apical meristem were proliferated more on medium with the addition of (0.25 and 0.5 mgL-1) BAP and (2 and 4 mgL-1) 2,4-D than the medium contain BAP (1 mgL-1) and 2,4-D (8 mgL-1). The present study, in F. vulgare MS media without any hormone was sufficient to regenerate the plantlet from the hypocotyls, roots and apical meristems explants. In MS medium supplemented with BAP (0.5, 2 and 4 mgL-1) and 2,4-D (0.2 mgL-1) shoots were formed earlier when the number of subculture was increased 4 times.
M.T. Darzi; A. Ghalavand; F. Sefidkon; F. Rejali
Volume 24, Issue 4 , February 2009, , Pages 396-413
Abstract
In order to study the effects of biofertilizers on quantity and quality of essential oil in fennel containing essential oil content and anethole content, fenchone content and limonene content in essential oil, an experiment was conducted at Hamand Research station in Damavand in 2005 and 2006. The factors ...
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In order to study the effects of biofertilizers on quantity and quality of essential oil in fennel containing essential oil content and anethole content, fenchone content and limonene content in essential oil, an experiment was conducted at Hamand Research station in Damavand in 2005 and 2006. The factors were mycorrhizal inoculation (inoculated and non-inoculated), phosphatic biofertilizer (0, 30, 60 kg/ha) and vermicompost (0, 5 , 10 ton/ha). The experiment design was factorial experiment in the base of randomized complete blocks design with eighteen treatments and three replications. In addition, one plot was allocated to control in each replication and only chemical fertilizers (NPK: 90, 60 and 90 kg/ha) were used. Data obtained from control plots were used for comparing other plots. Mean comparison was carried out using Duncan multiple range test (at 5% level). Results showed that the highest essential oil content in seed and anethole content in essential oil and the lowest fenchone content and limonene content in essential oil were obtained with mycorrhiza treatment. Phosphatic biofertilizer also showed significant effects on essential oil content and anethole and limonene contents in essential oil (except fenchone content). The maximum essential oil content in seed and anethole content in essential oil were related to the plots with consumption of 60 kg/ha. The lowest limonene content in essential oil was obtained with consumption of 30 kg/ha phosphatic biofertilizer. The highest essential oil content in seed and anethole content in essential oil and minimum fenchone content and limonene content in essential oil were obtained with consumption of 10 ton/ha vermicompost. Comparison of control versus biofertilizer treatments was significant, as two biofertilizer treatments include inoculation with mycorrhiza, application of 30 kg/ha phosphatic biofertilizer and 10 ton/ha vermicompost and inoculation with mycorrhiza and application of 60 kg/ha phosphatic biofertilizer and 10 ton/ha vermicompost in relation to quantity and quality of essential oil were better than control.
M.T. Darzi; A. Ghalavand; F. Rejali; F. Sefidkon
Volume 22, Issue 4 , February 2007, , Pages 276-292
Abstract
In order to study the effects of biofertilizers on yield and yield components in fennel, an experiment was conducted at Hamand Research Station in Damavand in 2005. The factors were mycorrhizal inoculation (inoculated and non-inoculated), biophosphate fertilizer (0, 30 , 60 kg/ha) and vermicompost (0 ...
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In order to study the effects of biofertilizers on yield and yield components in fennel, an experiment was conducted at Hamand Research Station in Damavand in 2005. The factors were mycorrhizal inoculation (inoculated and non-inoculated), biophosphate fertilizer (0, 30 , 60 kg/ha) and vermicompost (0 , 5 , 10 ton/ha). The experiment design was factorial experiment in the base of randomized complete blocks design with eighteen treatments and three replications. Also, these treatments with a fertilizer control treatment (NPK: 90, 60 and 90 kg/ha) was evaluated using a randomized complete blocks design with nineteen treatments and three replications. The measured traits were plant height, umbel no./plant, 1000 seed weight, biological yield, haevest index and seed yield. Mean comparison was conducted using duncan multiple range test (at 5% level). Results showed that the highest plant height, umbel no./plant, 1000 seed weight, biological yield and seed yield were obtained through mycorrhization but reduced harvest index. Biophosphate fertilizer also showed significant effects on plant height and biological yield. The maximum plant height and biological yield were obtained with consumption of 60 kg/ha from biophosphate fertilizer. The highest plant height, umbel no./plant, 1000 seed weight, biological yield and seed yield were obtained from vermicompost (10 ton/ha). Intractions of two factors of mycorrhizal inoculation and biophosphate fertilizer on 1000 seed weight and mycorrhizal inoculation and vermicompost on harvest index were significant. Comparison of control versus biofertilizer treatments was significant and umbel no./plant, biological yield and seed yield in two treatments of fifteenth (mycorrhizal inoculation-30 kg/ha biophosphate fertilizer-10 ton/ha vermicompost) and eighteenth (mycorrhizal inoculation-60 kg/ha biophosphate fertilizer-10 ton/ha vermicompost) from biofertilizer treatments were higher than that of control. There was a positive and significant correlation between seed yield with plant height, umbel no./plant, 1000 seed weight and biological yield.
A. Najafi Ashtiani; M.H. Lebaschi
Abstract
Due to correction of dry farming system and possibilities for replacement of low efficient plants, Fennel as a domestic, tolerant and valuable medicinal plant was used. Fennel (Foeniculum vulgare) is a perennial medicinal plant, which grows in 7 month per year. Its values are for medical, spice, health ...
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Due to correction of dry farming system and possibilities for replacement of low efficient plants, Fennel as a domestic, tolerant and valuable medicinal plant was used. Fennel (Foeniculum vulgare) is a perennial medicinal plant, which grows in 7 month per year. Its values are for medical, spice, health and soil conservation effects. One of the important components of Fennel is Trans-anethol, which is in the seeds. This experiment was carried out in Damavand dry land region with using a Complete Randomized Blocks Design (CRBD), in three replications. The treatments were three geographic orients with 50–55% slopes. The result shows a significant different among the seed yield which planted in the different orients. The highest yield obtains at third year with 1527 Kgha-1 in north orient and the lowest yield with 196 Kgha-1, which was related to the south orient in the first year. The north orient produced most seed yield with 1170 Kgha-1 in mean three years which was significant different with south orient. The 3 years growth of Fennel in Damavand region shows its dry farming possibility. Thus, Foeniculum vulgare could be cultivated in dry farming system as a tolerant medicinal plant, which produces economic seed yield.
R. Omidbaigi; K. Sadrai Menjili; F. Sefidkon
Abstract
To study the effect of sowing date on fennel CV. Soroksari, this research work was carried out on randomized complete block design with three replications. The effects of sowing dates of 6 of March, 20 of March, 4 of April, 19 of April, 5 of May, 21 of May, 5 of June and 21 of June, were studied on growth, ...
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To study the effect of sowing date on fennel CV. Soroksari, this research work was carried out on randomized complete block design with three replications. The effects of sowing dates of 6 of March, 20 of March, 4 of April, 19 of April, 5 of May, 21 of May, 5 of June and 21 of June, were studied on growth, development, seed yield and essential oil and anethole content of fennel CV. Soroksari. The results show that sowing dates hade significant effect on growth, development and quality and quantity of active substances of fennel. On the basis of the results the seeds were sown on 21 of May the seeds were germinated and developed faster. The highest plant (150.8 cm) and the largest number of branches per plant (10.1) was obtained from the plants were sown on 4 of April. There was no significant effect among 1000 seeds weight and essential oil content of different sowing dates. The largest seed yield (1864.3Kg/ha) and essential oil yield (91.3Kg/ha) was obtained from the seeds were sown on 4 of April. Anethole content were decreased from the plants were sown from 6 of March to 21 of June, the lowest amount (58.2%) was extracted from the plants were sown on 21 of June. According to the results of this resesrch trial the most suitable date for sowing of fennel cv. Soroksari is recommended 20 of March to 4 of April.
A. Akbarinia; M. Khosravifard; M.B. Rezaee; E. Sharifi Ashoorabadi
Abstract
In order to study the autumn and spring cultivation of Fennel (Foeniculum vulgare), Ajowan (Carum copticum), Black cumin (Nigella sativa) and Anis (Pimpenella anisum) under irrigation and non-irrigation conditions an experimental bulk design with 4 replications was conducted in Alamut medicinal ...
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In order to study the autumn and spring cultivation of Fennel (Foeniculum vulgare), Ajowan (Carum copticum), Black cumin (Nigella sativa) and Anis (Pimpenella anisum) under irrigation and non-irrigation conditions an experimental bulk design with 4 replications was conducted in Alamut medicinal plant research station in 2000 and 2001. Each medicinal plant was studied in a separate experiment. Treatments were included: autumn + irrigation, autumn + non–irrigation, spring + irrigation and spring + non-irrigation. The results showed significant different responses mentioned plants planting season Fennel and black cumin had earlier spring growth, more shoot, seed weight, and more seed yield because of better establishment in autumn. While ajowan and anis autumn planting could not be successful because of damping off, so their planting can only be practical in spring. All plants were better in respect of height; seed weight and seed yield in irrigation compared with non-irrigation condition. There was less difference between seed yield of fennel in irrigation and non-irrigation condition in autumn planting. Fennel and black cumin had 1703 and 1298 in irrigation, 948 and 447 kg/ha in non-irrigation condition in autumn planting. Also ajowan and anis had 1147, 1120 in irrigation and 312 and 361 kg/ha in non-irrigation condition in spring planting. Result of Pearson Correlation Coefficient about black cumin showed that there were significant correlations among all of the traits.
