A. Shafaghat
Abstract
One of the most important secondary metabolism products found in plants are flavonoids. They have medicinal and biological effects such as: purify blood, strengthen immune system, monitoring cholesterol level, regulate blood pressure, suppress acid secretion, prevent thrombus, suppress cytophy, antibacterial, ...
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One of the most important secondary metabolism products found in plants are flavonoids. They have medicinal and biological effects such as: purify blood, strengthen immune system, monitoring cholesterol level, regulate blood pressure, suppress acid secretion, prevent thrombus, suppress cytophy, antibacterial, prevent cancer, promote metabolism. In this study, the flavonoids and one chalcone from methanolic extract of flowers of Chrysanthemum parthenium (L.) Bernh. (syn. Tanacetum parthenium L.)(Compositae Family) were separated and purified by column chromatography and TLC methods. Kaempferol and naringenin are two flavonoids and trans- 4- ethoxy- 4'- methoxy chalcone as a chalcone compound were extracted, separated and detected by spectroscopy methods (1H-NMR, 13C-NMR, Mass and IR). Investigation of antioxidant activity included free radical scavenging activity towards 2, 2-diphenyl-1-pycrylhydrazile (DPPH) radicals. Methanolic extract of flowers of Tanacetum parthenium showed free radical scavenging activity.
G.H. Nasery; M. Mazandarani; M.B. Rezaee
Volume 24, Issue 2 , August 2008, , Pages 126-134
Abstract
Tanacetum parthenium L. is one of the most important medicinal herbs that grows wild and wide distribution near the river, road and sunny positions in forest and mountainous rangelands in South East of Golestan Province, especially in 800-1250m above the sea level in medium soil texture, neutral PH and ...
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Tanacetum parthenium L. is one of the most important medicinal herbs that grows wild and wide distribution near the river, road and sunny positions in forest and mountainous rangelands in South East of Golestan Province, especially in 800-1250m above the sea level in medium soil texture, neutral PH and organic carbon percent with medium to high. In addition to its ecological and ethnopharmacological needs essential oil composition of this plant were also studied. Artemisia annua, Artemisia absintium, Mentha longifolia, Mentha aquatica, Thymus carmanicus, Cuminum cyminum,Echium amoenum, andSalix alba for sedative of headache and menstrual pain, cold, fever, inflammation and healing wound. Flowering aerial parts of this plant were collected in July 2006 in 1100m of Ziarat mountainous region, 6km far from Gorgan city in Golestan province. The essential oil of different parts of plant obtained by water distillation and analyzed by GC/MS. Camphor (53.5%), a-pinene (17.7%) and a-bisabolol oxide B (10.5%) were the major components of flower oil. But in leaf oil, camphor (38.9%), a-pinene (8.4%), germacrene D (6.3%) and g-terpinene (8.2%) were the main components. The results confirm use of this plant by the rural healers for curing many types of the sedative pain and other ailments.
S. Akef; F. Bernard; H. Shaker; A.R. Ghasempoor
Abstract
In order to micropropagate and for acclimatization of Feverfew (Tanacetum parthenium), seeds were cultured on MS/4 medium with the whole vitamins, and shoot explants of seedlings, with shoot tip and without shoot tip, were cultured on MS medium supplemented with NAA (0.54µM) and BAP (4.44µM). ...
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In order to micropropagate and for acclimatization of Feverfew (Tanacetum parthenium), seeds were cultured on MS/4 medium with the whole vitamins, and shoot explants of seedlings, with shoot tip and without shoot tip, were cultured on MS medium supplemented with NAA (0.54µM) and BAP (4.44µM). 100% of explants with shoot tip and only 44% of explants without shoot tip produced plantlet. The number of plantlets produced, was more in shoot tip explants. For acclimatization, plantlets were rinsed with water for one hour. Roots were cut, and transferred to plastic pots containing Pit: Vermiculite (1:1). Plantlets were capable of acclimatization in ex vitro conditions and 62.5% of them remained alive.
S. Akef; F. Bernard; H. Shaker; A.R. Ghasem poor
Abstract
In this research investigation on parthenolide production and antioxidant defence in callus culture and micropropagated plantlets of feverfew (Tanacetum parthenium (L.) Sch. Bip. Family: Asteraceae) wounding, abscisic acid (ABA) and salicylic acid (SA) treatments were done. For this purpose, seeds were ...
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In this research investigation on parthenolide production and antioxidant defence in callus culture and micropropagated plantlets of feverfew (Tanacetum parthenium (L.) Sch. Bip. Family: Asteraceae) wounding, abscisic acid (ABA) and salicylic acid (SA) treatments were done. For this purpose, seeds were cultured on MS/4 medium with the whole vitamins, and shoot explants of seedlings, with shoot tip and without shoot tip and also, were cultured on MS medium supplemented with NAA (0.54µM) and BAP (4.44µM). The growth of plantlets was computed as dry weight. For biochemical investigations, shoot tip explants were cut from two months old plantlets and treated with ABA, SA and wounding (leaves discarded) for 10 hours in MS liquid medium. Parthenolide content was measured by HPLC. Antioxidant defense was evaluated as qualitative peroxidase activity. Callus tissues were obtained from leaf explants of seedlings, in MS medium supplemented with NAA (2mg.L-1) and BAP (0.5mg.L-1). One month old calli were transferred to liquid medium with ABA, SA and DMSO treatments. After one weak, parthenolide content and peroxidase activity were measured as above. Parthenolide content of control plantlets in this investigation was 0.00863±0.00283, but SA + Wounding treatment in plantlets showed the less parthenolide content and the maximum dry weight. Parthenolide production in treatments was decreased as the growth increased. In callus tissues, parthenolide content was not different significantly between treatments, and approximately half of the plantlets. Qualitative variation in antioxidant defense was shown by electrophoretic patterns and we had an extra band in wounding treatment in micropropagated plantlets. Anionic bands were stronger than cationic bands. Electrophoretic bands had no difference in callus tissues.
