S. Khalili; A. Moieni; M. Abdoli
Abstract
Echinacea is one of the most important medicinal plants and an important source of cichoric acid. In this research, callus induction, indirect shoot regeneration, and shoot rooting of Echinacea angustifolia DC. were investigated. The effects of explant (leaf, petiole and root) and NAA concentrations ...
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Echinacea is one of the most important medicinal plants and an important source of cichoric acid. In this research, callus induction, indirect shoot regeneration, and shoot rooting of Echinacea angustifolia DC. were investigated. The effects of explant (leaf, petiole and root) and NAA concentrations (0.5, 1 and 1.5 mg l-1) on callus induction as well as the effects of explant (leaf, petiole and root) and BAP concentrations (3, 4 and 5 mg l-1) on the indirect shoot regeneration of callus were evaluated in two separated experiments. The study was conducted as factorial in a completely randomized design (CRD). The effects of IBA concentrations (0.5, 1, 1.5 and 2 mg l-1) on shoot rooting were also investigated in a completely randomized design. The effects of the factors studied on the callogenesis were not significant while these factors had significant effects on the shoot regeneration and rooting at 1% probability level. In general, the results indicated that the highest indirect shoot regeneration (72%) was obtained from the leaf explant and by the use of 4 mgl-1 BAP. In addition, the highest shoot rooting (38% and 40%) was obtained using 1 and 1.5 mgl-1 IBA. The good potential of this species for indirect shoot regeneration was shown in this research. These results can be used in micropropagation programs, gene transfer, and transgenic plants production.
L. Elyasi; A.A. Mehrabi; M. Seyedi; Z. Safari
Abstract
Satureja bachtiarica L., belonging to the Lamiaceae family, is rich in essential oils, used for various purposes including pharmaceutical, food, and health applications. In vitro culture of Satureja, in order to callus induction and optimization of cell suspension, provides rapid production and easy ...
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Satureja bachtiarica L., belonging to the Lamiaceae family, is rich in essential oils, used for various purposes including pharmaceutical, food, and health applications. In vitro culture of Satureja, in order to callus induction and optimization of cell suspension, provides rapid production and easy extraction of secondary metabolites. In the present study, callus induction was conducted by using different explants and concentrations of growth regulators. The most suitable calli were selected to induce suspension cultures and were transferred to liquid media supplemented with different combination of BAP and NAA. The experiment was performed in a factorial completely randomized design by using three replications. The cell biomass (cell number) was examined on different days. The mean comparison results for the explant origin showed that the internode explant produced the highest cell number. In addition, evaluation of interaction of explants with media showed that the maximum cell biomass was obtained by internode explant and the medium containing 2mg.L-1 NAA plus 0.5mg.L-1 BAP in the fifth day. On the other hand, study of growth process and cell proliferation during the days after the establishment of suspension cultures, also revealed that this hormonal composition had the highest utility to generate the maximum number of cells in all explants tested.
R. Shahhoseini; M. Moghaddam; D. Kiani; R. Mansori
Abstract
Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD ...
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Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD (Randomized Complete Block Design) with six treatments (0, 1000, 2000, 3000, 4000 and 5000 mg l-1) and four replications in order to evaluate the effects of different concentrations of IBA and NAA on rooting of semi-hardwood cuttings of rosemary. Cuttings were treated in solutions of IBA and NAA for 1 min and held in plastic tunnel under mist system. After 70 days, the percentage of rotting, number of roots, average root length, the root fresh and dry weight, length of cutting, leaves and stem fresh and dry weight were measured. The analysis of variance showed that application of IBA and NAA had a significant effect on all the parameters studied. The highest rooting percentage was obtained at concentration of 1000 mg l-1 NAA (84%) and 5000 mg l-1 IBA (66%). The concentration of 4000 mgl-1 IBA was the best treatment on quality and rooting of the semi-hardwood cuttings of rosemary.
M.H. Assareh; Z. Abravesh; S.R. Tabaei Aghdaei
Abstract
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature ...
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Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature shrubs. The branches were laid in sand bed, soaked for 24 h by different concentrations of IBA and NAA hormones. The results showed that the rate of rooting was high in sand bedding material in autumn and winter. The highest rooting percentage was %100 with 50 mgl-1 concentration of IBA in autumn and winter seasons and the lowest of rooting percentage was %53.33 with 50 mgl-1 concentration of NAA in autumn season. The rooting branches were cut from the parent plants after 3 months and planted in pots containing soil: sand: peatmoss complex (1:1:1) in greenhouse conditions, survival percentage was 90%.
