Improvement and breeding
M. Moghadami Rad; R.A. Khavari-Nejad; S. Saadatmand; F. Najafi
Abstract
Madder (Rubia tinctorum L.) is a herbaceous and perennial plant, which has bioactive compounds with high medicinal and pigment potency. Local farmers believe that increasing soil lime increases the color composition of this plant. To investigate the effects of calcium carbonate and phosphorus fertilizer ...
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Madder (Rubia tinctorum L.) is a herbaceous and perennial plant, which has bioactive compounds with high medicinal and pigment potency. Local farmers believe that increasing soil lime increases the color composition of this plant. To investigate the effects of calcium carbonate and phosphorus fertilizer on some physiological parameters and root alizarin content in madder, a field factorial experiment was conducted in a randomized complete block design with four replications. Experimental treatments included calcium carbonate at five levels (0, 20, 50, 100, and 150 kg.ha-1) and triple superphosphate at four levels (0, 50, 100, and 150 kg.ha-1). Means comparison showed that the highest root dry weight (an increase of 75% compared to control), total plant dry weight (an increase of 58% compared to control), root alizarin content (an increase of 78% compared to control), and root catalase activity were obtained with calcium carbonate increase up to 20 kg.ha-1 and triple superphosphate increase up to 100 kg.ha-1. The highest root length (an increase of 54% compared to control) and leaf catalase activity were observed in the 50 kg.ha-1 calcium carbonate and 100 kg.ha-1 triple superphosphate treatment. The highest leaf and root peroxidase activity was obtained in the treatment of 150 kg.ha-1 calcium carbonate and 150 kg.ha-1 triple superphosphate. Simultaneous application of these two fertilizers had a complementary effect on each other, so that the 20 and 50 kg.ha-1 calcium carbonate application along with the 100 kg.ha-1 triple superphosphate had an increasing effect on the alizarin content of madder root. This method can be used for the future development of a non-destructive tolerance test that shows madder increased response to calcium carbonate and phosphorus fertilizer, to further produce madder root medicinal compounds as a targeted drug for cancer treatment.
A. Rahamani; M. Mirza; S.R. Tabaei-Aghdai
Abstract
In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications ...
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In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications in a loam-clay soil with pH =7.9. In this experiment, nitrogen, phosphorus and potash and a combination of trace elements as fertilizer and cow manure were used. Amount of essential oil and quality of essential oil at different treatments were measured in 3 years. Essential oils of petals were extracted by hydrodistillation method in three consecutive years and oil composition was identified in the first and second years of flowering stage using GC and GC/MS. The results of analysis of variance and mean performance showed that the difference among treatments was significant at 1% level of probability. Treatment N80P80K40 and 30 tons of manure per hectare had the highest amount of essential oil. The average amount of essential oil in three consecutive years in this with 0.35 percent was the highest and in control with 0.23 percent was the lowest value. The highest average oil yield in the three years was recorded for treatment N40P40K40, (1395.58 g/ha), and minimum was obtained for the treatment consisting only 40 tons of manure, (636.23 g/ha). After that, the minimum oil yield was recorded for control treatment (868.98 g/ha) in these three years. The composition of essential oils in the first year of flowering showed that Linalool, Geraniol and Citronelllol increased 88, 39 and 36 percent, respectively in treatment N40P40K40, compared with the control. In the second year of flowering, the amount of Geraniol in this treatment increased by 45 percent and that of hexadekanol and heptadekan decreased 45 percent and 30 percent compared with the control.
