A.R. Yavari; V. Nazeri; F. Sefidkon; Z. Zamani; M.E. Hassani
Abstract
In the present study, the genetic variation within and among some populations of Thymus migricus Klokov & Desj.-Shost was investigated using the RAPD markers. Twenty-one decamer RAPD primers produced 310 unique bands. RAPD analysis showed 14 monomorphic and 296 polymorphic bands in different genotypes. ...
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In the present study, the genetic variation within and among some populations of Thymus migricus Klokov & Desj.-Shost was investigated using the RAPD markers. Twenty-one decamer RAPD primers produced 310 unique bands. RAPD analysis showed 14 monomorphic and 296 polymorphic bands in different genotypes. The number of polymorphic bands per primer varied from 5 to 20 with a mean of 14.09. Genetic distance was measured by Nei’s coefficient and cluster analysis was carried out. A dendrogram was drawn based on genetic distance data, applying the UPGMA clustering method. Analysis of molecular variance (AMOVA) was performed. According to the dendrogram, among T. migricus populations, Band and Jolfa populations had maximum differences with a distance of 0.130. Evaluation of genetic diversity within populations with an average of Nei’s gene diversity analysis and Shannon’s information index, showed that diversity within population of Jolfa (h = 0.196 & I = 0.294) was more than other populations while genetic diversity within population of Ghushchi (h = 0.139 & I = 0.209) was less than other populations. Mean of Fst and Nm indexes which show gene flow among populations, were 0.30 and 1.14, indicating a greater gene flow among five populations of T. migricus. The results of the present study showed that there was a greater level of genetic variation in the Iranian natural populations of T. migricus which could be applied for future breeding programs.
A.R. Yavari; V. Nazeri; F. Sefidkon; M.E. Hassani
Abstract
Thymus migricus Klokov & Desj.-Shost.is one of the 14 species of genus Thymus L. which grows naturally in Iran. In this study, natural habitats of this species were identified during travels to different regions in East and West Azerbaijan provinces in the early of growth season in 2008. Five complete ...
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Thymus migricus Klokov & Desj.-Shost.is one of the 14 species of genus Thymus L. which grows naturally in Iran. In this study, natural habitats of this species were identified during travels to different regions in East and West Azerbaijan provinces in the early of growth season in 2008. Five complete plants were collected from five localities in order to study and evaluate quantitative morphological characters. To study seed character, inflorescences were collected at the time of seed production. Flowering stems were used to investigate essential oil quantitatively. Data of locations, vegetative and reproductive characters of every population and amount of essential oils of each location were recorded. The results were analyzed using cluster analysis method with SPSS software. Populations were clustered based on vegetative, reproductive characters and essential oil amount. All populations were classified into 3 groups. "Band", "Nazloo" and "Ghushchi" populations in a same group, "Heris" clustered separately. Finally "Jolfa" population was placed in independent group. Specimens from "Jolfa" and "Nazloo" showed longest flowering stems, largest leaves and flowers and also highest essential oil production, therefore it is recommended for breeding programs in cold regions of northwest Iran.
A.R. Yavari; V. Nazeri; F. Sefidkon; M.E. Hassani
Abstract
Genus Thymus (Lamiaceae) consists of about 250 species. Fourteen species grow naturally in Iran of which four are endemic. Different species of Thymus are used as tonic, carminative, digestive, antispasmodic, anti-inflammatory, antitussive, expectorant and for the cold treatment in Iranian traditional ...
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Genus Thymus (Lamiaceae) consists of about 250 species. Fourteen species grow naturally in Iran of which four are endemic. Different species of Thymus are used as tonic, carminative, digestive, antispasmodic, anti-inflammatory, antitussive, expectorant and for the cold treatment in Iranian traditional medicine. Chemical composition of essential oil of T. migricus Klokov & Desj.-Shost., a species grows in northwest of Iran, has been investigated in this paper. The aerial parts were collected from Ghushchi, Nazloo and Band regions from West Azerbaijan province in the middle of May 2008. Then air-dried flowering stems of the plant were submitted to hydrodistillation using a Clevenger-type apparatus according to the method recommended in British Pharmacopoeia. The essential oil was analyzed by combination of GC-FID and GC-MS. The total yields of oils based on dry weight were 1.5%, 1.7% and 1.4% (w/w) from Ghushchi, Nazloo and Band, respectively. Twenty, Eighteen and Twenty-one compounds were identified from Ghushchi, Nazloo and Band, respectively among which thymol (46.6-70.5%), γ-terpinene (6.2-16.7%) and p-cymene (4.0-6.2%) have been the three major and common constituents and also had high concentrations. With respect to high yield of essential oil and valuable phenolic compound in Nazloo specimen, it is suggested for medicinal applications.
