Agriculture and horticulture
niloufar Rezaei; Fatemeh Sefidkon; hassan ali naghdibadi
Abstract
The cultivation of medicinal plants such as the Lamiaceae family increasing throughout the world Significantly. Savory considers as an important species in this family which it’s important is due to carvacrol and thymol in essential oil. In order to investigate the effect of planting density ...
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The cultivation of medicinal plants such as the Lamiaceae family increasing throughout the world Significantly. Savory considers as an important species in this family which it’s important is due to carvacrol and thymol in essential oil. In order to investigate the effect of planting density and soil fertility on the physiological traits and antioxidant enzymes activity of savory under rainfed conditions, an experiment in the form of split plots with two factors, fertilizer as the main factor at three levels (control, 30 tons/ha cow manure and 10 tons/ha straw enriched with ammonium sulfate) and the sub-factor, plant density at three levels (2.67, 4 and 8 plants/m2) in the form of a complete block design A random experiment was conducted at Hamand Absard research station (Damavand) in 2017-18 and 2018-19. The analysis of variance showed a significant effect of planting density and organic fertilizers on the physiological traits of savory. The highest RWC (%87.67) and the lowest ELI (%47.19) were observed in the cow manure treatment with 30 tons/ha at a density of 2.67 plant/m2. As the density increased, total chlorophyll and RWC decreased and ELI and MDA and proline increased. Cow manure and straw increased total chlorophyll, proline and RWC and decreased ELI and MDA. The highest activity of CAT, peroxidase and SOD enzyme was obtained in the control treatment and 8 plant/m2 and the lowest in organic fertilizer treatment, 2.67 plant/m2. Increasing the activity of these enzymes to neutralize the reactive oxygen species produced due to water stress is known as a resistance mechanism in plants. Based on the results of this research, it can be said that savory has the ability to grow in rain-fed conditions and has the necessary potential to increase the antioxidant capacity in rainfed conditions.
Improvement and breeding
- -; Masomeh Moradi; Mousa Torabi Giglou; Mahsa Ahadzadeh
Abstract
Background and objectives: Basil (Ocimum basilicum L.) is a plant from the Mint family and the genus Ocimum, which has many species, among which O. basilicum is the most useful economic species, and like other plants of the Mint family, it is a source of cyclic compounds and ...
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Background and objectives: Basil (Ocimum basilicum L.) is a plant from the Mint family and the genus Ocimum, which has many species, among which O. basilicum is the most useful economic species, and like other plants of the Mint family, it is a source of cyclic compounds and essential oil. It is also known as a rich source of phenolic compounds (especially rosmarinic acid and caffeic acid) and flavonoids. The plant's vegetative body contains essential oils. Linalool, methyl chavicol, citral, euginol, cineol, geraniol, camphor, and methyl cinnamate are important components of basil essential oil. As the most critical abiotic stress, drought stress plays a crucial role in reducing the yield of medicinal plants. It causes numerous morpho-physiological and biochemical changes in the plant. The performance of the effective substance is influenced by the plant's economic performance and environmental conditions, including drought stress. Stress effects are different at different stages of growth and development.Methodology: To investigate the effect of ascorbic acid on native O.basilicum the native population "Shahre-ray," under drought stress conditions, a factorial experiment in the form of a randomized complete block design was performed in four replications on the research farm of Horticulture Department of Mohaghegh Ardabili University in 2017. Treatments included drought stress (complete irrigation and cessation of irrigation at the beginning of reproductive growth and cessation of irrigation at 50% flowering) and ascorbic acid foliar application at four concentrations of 0.0, 0.5, 1, and 2 mM. This experiment was field operated in May 2016. Before the experiment, the bed preparation operation included plowing, disking, and leveling the ground. The plots of one meter in length and width were created. The seeds were planted in three lines with 15 cm plant spacing. 3-4 cm of rotted manure was used to cover the seeds. After determining the soil texture by the hydrometric method, the mentioned treatments were applied to stress. On the first day, irrigation was a flood, and after germination, irrigation was reduced. All traits were measured in the Physiology Laboratory after the Horticulture Science Department of the Faculty of Agricultural Sciences analyzed the data with SAS software. Also, the treatment averages were compared with Duncan's multi-range test at a probability level of 5%. Results: The results showed that with increasing drought stress intensity, total plant height, stem length, number of leaves, and plant dry weight decreased by 36.08%, 39.75%, 51.26%, and 53.80% compared to the control treatment, respectively. If ascorbic acid spraying could improve these values, the highest root length was obtained under severe stress conditions (beginning of flowering) and treatment with ascorbic acid (15.83 cm), which showed an increase of about 31.91% compared to the control treatment. Drought stress conditions increased proline and essential oil components. Chlorophyll content also decreased under severe stress. Also, by applying drought stress, in all studied traits, the highest amount of ascorbic acid treatment (concentrations of 0.5 and 1 mM) increased, and higher concentrations decreased the content of studied traits. By analyzing the essential oil of plant samples, the main compounds obtained from basil essential oil included geraniol, methyl chavicol, alphapinene, caryophyllene, citral, coupon, carvacrol, neral, linalool, and limonene. In most compounds, essential oil compounds and components were enhanced by increasing drought stress and spraying. The highest amount of essential oil in the stress condition up to the 50% flowering stage and sprayed with 2 mM ascorbic acid was obtained for methyl chavicol compound (33.76%).Conclusion: Applying ascorbic acid increased basil growth under drought stress and created resistance in the plant. Therefore, due to the lack of water resources and the great need of plants for water, it is possible to create resistance to stress in plants by spraying non-toxic and essential elements on them.
Agriculture and horticulture
H. Kiani; Sh. Khalesro; A. Mokhatssi-Bidgoli; Z. Sharifi
Abstract
Nigella sativa L. is a valuable medicinal plant that is widely used in different industries. Accumulation of compatible osmolytes is one of the common responses of plants under drought stress. To investigate the effects of irrigation regimes and biochar (resulting from the heating of cattle manure) on ...
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Nigella sativa L. is a valuable medicinal plant that is widely used in different industries. Accumulation of compatible osmolytes is one of the common responses of plants under drought stress. To investigate the effects of irrigation regimes and biochar (resulting from the heating of cattle manure) on N. sativa, a factorial experiment was conducted in a completely randomized design with three replications in the greenhouse of the Faculty of Agriculture at the University of Kurdistan in 2018. The experimental factors consisted of three drought stress levels (40, 70, and 100% of FC) and two biochar use levels (0 and 15 tons.ha-1). The ANOVA results showed that the interaction effects of drought stress and biochar were significant on hydrogen peroxide, malondialdehyde, superoxide dismutase, peroxidase, proline, soluble carbohydrates (water and ethanol soluble), and osmotic potential. Increasing the intensity of drought stress enhanced the amount of hydrogen peroxide, malondialdehyde, superoxide dismutase, peroxidase, proline, and soluble carbohydrates (water and ethanol soluble) and caused the osmotic potential to become more negative. Biochar application decreased the negative effects of drought stress so that hydrogen peroxide, malondialdehyde, superoxide dismutase, peroxidase, proline, and soluble carbohydrates (water and ethanol soluble) amounts were lower than the treatments without biochar. Overall, the present research results proved the useful and effective role of biochar in improving the physiological traits and protective osmolytes of N. sativa under drought stress.
Agriculture and horticulture
M.H. Rad; R. YazdaniBiouki; V. Soltany gerdeframarzi; N. Besharat
Abstract
To investigate the effects of irrigation water salinity (3, 6, 9, 12, and 15 dS.m-1) on morphophysiological characteristics of seedless barberry (Berberis vulgaris L.) aerial parts, an experiment was conducted using drained big pots in a completely randomized design with three replications at the central ...
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To investigate the effects of irrigation water salinity (3, 6, 9, 12, and 15 dS.m-1) on morphophysiological characteristics of seedless barberry (Berberis vulgaris L.) aerial parts, an experiment was conducted using drained big pots in a completely randomized design with three replications at the central station of National Salinity Research Center, Yazd during 2018-2021 years. The salinity levels affected the height, diameter, area, and volume of plants canopy, number of new branches, number of new basal shoots, number of leaves per plant, total leaves area, leaf area index, leaf dry weight, leaves damage percentage at high temperature, amount of total chlorophyll, chlorophylls a and b, carotenoids, proline, total sugar, leaf anthocyanins, leaf phenols, and antioxidant activity significantly (P<0.01). Based on the results, B. vulgaris tolerated the salinity stress well. Despite the growth reduction at salinity levels above 9 dS.m-1, plant physiological resistance was observed through the production of secondary osmolytes and survival under saline conditions up to 12 dS.m-1. Accordingly, in suitable barberry production habitats, it is possible to take advantage of water resources and saline soil to grow seedless barberry, especially to increase phenolics, by ture management of the root environment and control of the saturated soil extract salinity up to 12 dS.m-1.
Agriculture and horticulture
R. Rostami; B. Esmaielpour; S.A. Hosseini; G. Salimi; A. Etminan
Abstract
To investigate the effects of mycorrhizal fungi on growth characteristics, physiology, and essential oil yield of thyme (Thymus vulgaris L.) under lead (Pb) stress, a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of University ...
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To investigate the effects of mycorrhizal fungi on growth characteristics, physiology, and essential oil yield of thyme (Thymus vulgaris L.) under lead (Pb) stress, a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of University of Mohaghegh Ardabili (UMA) in 2018. Experimental treatments included different levels of Pb heavy metal (0, 200, and 400 ppm) and inoculation with mycorrhizal fungi (without inoculation, inoculation with Funneliformis mosseae, and Claroideoglomus etunicatum). The results showed that in comparison with the control, with increasing Pb concentration in the soil, plant height (40.56%), number of leaves (36.09%), stem dry weight (43.50%), essential oil percentage (40%), and cell membrane stability (16.14%) were significantly decreased, while proline content (51.72%) and catalase activity (45%) of the leaves increased. Inoculation of thyme with the mycorrhizal fungi was effective in increasing the vegetative indices in presence of Pb heavy metal so that both G. mosseae and G. etunicatum increased the number of leaves by 66.20 and 9.55% compared to the control, respectively. Also, inoculation with G. mosseae under severe Pb stress conditions (400 ppm) increased leaf proline content (11.49%), peroxidase enzyme activity (8.8%), chlorophyll b (21%), total chlorophyll (70.43%), carotenoids (19.6%), and essential oil percentage (55%) compared to the control. Overall, both mycorrhizal fungi were effective on alleviation of Pb toxicity effects and F. mosseae had better performance, especially in terms of morphological traits and essential oil yield. Due to the negative effects of heavy elements on human health, the recommendations to use mycorrhizal fungi to reduce the absorption of heavy metals in plants should be done with more consideration of all aspects including the plant and fungus species type and rate and pollution type.
Agriculture and horticulture
A. Abbasi Khammar; M. Moghaddam; A. Asgharzade; M. Mahmoodi Sourestani
Abstract
To study the effects of different nitrogen levels (0, 70, 140, and 210 mg l-1) on some growth, biochemical, and nutrient uptake (nitrogen, phosphorus, and potassium) characteristics and essential oil content in sage (Salvia officinalis L.) under hydroponic conditions, a pot experiment was conducted based ...
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To study the effects of different nitrogen levels (0, 70, 140, and 210 mg l-1) on some growth, biochemical, and nutrient uptake (nitrogen, phosphorus, and potassium) characteristics and essential oil content in sage (Salvia officinalis L.) under hydroponic conditions, a pot experiment was conducted based on a completely randomized design with four replications in the research greenhouse of Ferdowsi University of Mashhad in 2019. The results of means comparison showed that with increasing the nitrogen level, the plant growth characteristics increased, so that the 210 mg l-1 N treatment decreased the stem diameter, number of branches, nodes, and leaves, length of internodes, length and leaf width, fresh and stem dry weight, fresh and aerial parts dry weight, and aerial parts dry weight to root dry weight ratio by 54.19, 72.59, 39.49, 82.52, 51.42, 72.20, 71.46, 95.17, 90.31, 95.82, 92.30, and 83.46%, respectively compared to the no N application. The application of 210 mg l-1 N increased the relative content of leaf water (RWC), proline, and essential oil content by 9.57, 266.6, and 14.87% and decreased the electrolyte leakage and malondialdehyde by 30.45 and 51.51%, respectively compared to the no N application. Also, the lowest N level decreased the K, P, and N content by 32.65, 85.13, and 56.89%, respectively compared to the highest N level. The results of this experiment showed that different N levels could have different effects on the growth and essential oil content of sage. Based on the present research findings, the application of 210 mg l-1 N could be recommended for the sage cultivation due to the improving effects on the studied traits of the plant.
Agriculture and horticulture
A. Hayati; M.M. Rahimi; A. Kelidari; S.M. Hosseini
Abstract
Black cumin (Nigella sativa L.) seeds are used in the traditional medicine in many countries to prevent and treat many disorders and diseases including cough, asthma, nasal congestion, headache, toothache, intestinal worms, menstrual disorders, gastrointestinal diseases, and impotence. To study the effects ...
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Black cumin (Nigella sativa L.) seeds are used in the traditional medicine in many countries to prevent and treat many disorders and diseases including cough, asthma, nasal congestion, headache, toothache, intestinal worms, menstrual disorders, gastrointestinal diseases, and impotence. To study the effects of humic acid and iron nanochelate on the content of osmotic protective osmolites including glucose, fructose, sucrose, proline, superoxide dismutase, catalase, and peroxidase of medicinal plant black cumin under the drought stress conditions, an experiment was conducted as a split factorial based on the randomized complete block design with three treatments in three replications at the Agricultural and Natural Resources Research Station of Eqlid city during two crop years of 2018 and 2019. The main plot consisted of three levels of irrigation (50, 75, and 100% of available water) and sub-plots included humic acid (0, 250, and 500 mg l-1) and iron nanochelate (0, 1, and 2 g l-1). The results showed that the effects of irrigation, humic acid, and iron chelate treatments on the catalase, superoxide dismutase, glucose, and peroxidase content, irrigation treatments on the proline content, and irrigation and humic acid treatments on the sucrose and fructose content were significant. The content of all protective osmolites increased in the 50% drought stress treatment. The highest content of glucose, fructose, and sucrose was obtained in the 50% drought stress, 500 mg l-1 humic acid, and 2 g l-1 iron nanochelate treatment. Overall, based on the results of this experiment, the application of humic acid fertilizer (500 mg l-1) and iron nanochelate (2 g l-1) could be recommended to reduce the effects of drought stress on black cumin.
Agriculture and horticulture
P. Arvin; R. Firuzeh
Abstract
Salinity is one of the most important and common environmental stresses that affects the quantitative and qualitative yield of many plants. Fenugreek (Trigonella foenum-graecum L.) is one of the most well-known medicinal plants, which is used in the cure of many diseases due to its various effective ...
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Salinity is one of the most important and common environmental stresses that affects the quantitative and qualitative yield of many plants. Fenugreek (Trigonella foenum-graecum L.) is one of the most well-known medicinal plants, which is used in the cure of many diseases due to its various effective compounds such as trigonelline. This study was aimed at investigating the effects of salinity stress on the yield components and some physiological and biochemical characteristics of four fenugreek populations under the greenhouse conditions. The pot factorial experiment was conducted in a completely randomized design with three replications. The experimental factors included four populations (Neishabour, Shirvan, Shiraz, and Ardakan) and four salinity levels (0, 30, 60, and 90 mM). At the final stages of growth, the traits including the relative water content (RWC), number of pods per plant, number of seeds per pod, 1000-seed weight, and content of soluble sugars, proline, and trigonelline were measured in the mature plants. The salinity stress decreased the yield traits such as the number of pods per plant, number of seeds per pod, and 1000-seed weight in the all populations studied. The results showed that the salinity stress also decreased the concentration of soluble sugars and RWC, but increased the content of proline and trigonelline in the seeds. The population Shiraz was superior to the others in terms of the traits studied and showed more efficient mechanisms to tolerate the stress conditions.
Improvement and breeding
A. Barghi; A. Gholipouri
Abstract
Salinity is one of the most common abiotic stresses, which causes a high decrease in crop production. Growth regulators play an important role in plant growth and development under normal and stress conditions. To study the effects of foliar application of jasmonic acid and 24-epibrassinolide on yield ...
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Salinity is one of the most common abiotic stresses, which causes a high decrease in crop production. Growth regulators play an important role in plant growth and development under normal and stress conditions. To study the effects of foliar application of jasmonic acid and 24-epibrassinolide on yield and yield components, oil and essential oil percentage and some physiological characteristics of black cumin (Nigella sativa L.) under salinity stress conditions, a factorial experiment based on the randomized complete block design was conducted in the research greenhouse of Mohaghegh Ardabili University with three replications. The treatments included salinity at four levels (0, 5, 10 and, 15 ds.m-1 NaCl) and plant growth regulators at three levels (control, 0.1 mmol 24-epibrassinolide, and 0.5 mmol jasmonic acid). Results of mean comparisons indicated that salinity stress decreased plant yield, number of seeds per capsule, number of capsules per plant, and seed oil percentage, while significantly increased 1000-seed weight and proline content. Growth regulators also increased 1000-seed weight and seed oil percentage and decreased proline content. Chlorophylls a and b contents of the leaves decreased with increasing salinity levels, and the use of jasmonic acid and 24-epibrassinolide increased their amounts at all salinity levels. Malondialdehyde content increased with increasing salinity levels, and plant growth regulators foliar spray decreased its content at all stress levels. Besides, the percentage of seed essential oil increased with increasing salinity levels and also with plant growth regulators application at all salinity levels. It is recommended to foliar spray the plant with 24-epibrassinolide and jasmonic acid growth regulators under salinity conditions to avoid stress damages and increase black cumin essential oil, which is a very important compound in the pharmaceutical industry, as well as to enhance seed oil percentage, 1000-seed weight, and photosynthetic pigments of black cumin.
Improvement and breeding
B. Esmaielpour; M. Shiekhalipour; M. Torabi-Giglo
Abstract
Soil salinity in arid and semi-arid regions is one of the most important abiotic stresses causing negative effects on the growth and yield of medicinal plants. To investigate the effect of Zn nanoparticles foliar spray at different levels on growth characteristic, antioxidant enzymes, and essential oil ...
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Soil salinity in arid and semi-arid regions is one of the most important abiotic stresses causing negative effects on the growth and yield of medicinal plants. To investigate the effect of Zn nanoparticles foliar spray at different levels on growth characteristic, antioxidant enzymes, and essential oil yield of dragonhead (Dracocephalum moldavica L.) under salinity stress conditions, a factorial experiment was conducted in a completely randomized design with three replications in the greenhouse of Mohaghegh Ardabili University during 2018-2019. Experimental treatments included salinity stress at four levels (0, 50, 100, and 150 mM of NaCl) and foliar spray with Zn nanoparticles at three levels (0, 100, and 500 mg.l-1). Traits including plant height, aerial parts fresh and dry weight, chlorophyll, cell membrane electrolyte leakage, relative water content, proline, antioxidant enzymes, and percentage and yield of essential oil were measured. Results showed that salinity significantly decreased plant height, shoot fresh and dry weight, chlorophyll, the relative water content of leaves, and essential oil yield, and increased electrolyte leakage from cell membranes and proline content of the leaves. While nano-Zn foliar spray reduced the negative effects of salinity stress by increasing the growth and activity of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase. Treatment of 500 mg.l-1 nano-Zn caused the best-improving effect on traits including plant height, stem dry weight, chlorophyll, electrolyte leakage, antioxidant enzymes, and essential oil yield under salinity stress conditions. Therefore, the use of 500 mg.l-1 nano-Zn is recommended as a reduction of the negative effects of salinity stress in dragonhead.
A. Rahbary; H. Fatemi; B. Esmailpour
Abstract
Recently, various methods have been used to alleviate the detrimental effects of pollutants. The application of biofertilizers with respect to their positive impacts on soil characteristics and environment can be served as a suitable alternative to many of these methods. In order to investigate the two-way ...
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Recently, various methods have been used to alleviate the detrimental effects of pollutants. The application of biofertilizers with respect to their positive impacts on soil characteristics and environment can be served as a suitable alternative to many of these methods. In order to investigate the two-way effect of bacterium Pseudomonas fluorescens on plant growth, physiological and biochemical characteristics of dill (Anethum graveolens L.) under Pb stress conditions, a factorial experiment based on a completely randomized design with three replications was conducted at Mohaghegh Ardabili University. Experimental treatments included Pb stress at two levels (0 and 600 ppm) and bacterial inoculation at three levels (inoculation with Pseudomonas fluorescens strains150 and 159 separately and without inoculation (control)). The results of this study showed that the growth characteristics such as leaf number, plant height, and shoot fresh and dry weight were significantly affected by Pb contamination and shoot fresh and dry weight, leaf number and plant height decreased by 22, 31, 19 and 9%, respectively. The content of proline and carbohydrates in dill showed a significant increase under the influence of lead. Bacterial inoculation also increased the content of proline and plant carbohydrates, which was more pronounced in inoculation with strain150. The activity of antioxidant enzymes was significantly influenced by Pb contamination and bacterial inoculation. Pb increased peroxidase but decreased catalase activity, but inoculation with Pseudomonas bacteria, especially strain 150, significantly increased the activity of both enzymes. In addition, the bacteria were able to reduce the amount of Pb in dill. In general, the results of this experiment indicated that Pseudomonas bacterial inoculation was effective in alleviating the effects of Pb stress on dill via growth promotion and improving enzymatic activity.
Y. Mehrabi; M. Movahhedi Dehnavi; A. Salehi; R. Mohatashami; M. Hamidian
Abstract
In order to investigate the effects of different levels of supplementary irrigation and bio-fertilizers on some physiological traits, yield, and essential oil percentage of medicinal plant cumin (Cuminum cyminum L.), an experiment was conducted as split-plot in a randomized complete block design ...
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In order to investigate the effects of different levels of supplementary irrigation and bio-fertilizers on some physiological traits, yield, and essential oil percentage of medicinal plant cumin (Cuminum cyminum L.), an experiment was conducted as split-plot in a randomized complete block design with three replications in the research field of Kohgiluyeh and Buyer-Ahmad Agricultural and Natural Resources Research and Training Center in 2017. The main plot included supplementary irrigation (complete rainfed conditions, supplementary irrigation at 50% flowering and at the start of seed formation) and the subplot included bio-fertilizer (no phosphorus bio-fertilizer, mycorrhiza Glomus mosseae, phosphorus bio-fertilizer, and combination of mycorrhiza and phosphorus bio-fertilizer). The application of supplementary irrigation with bio-fertilizer increased the total chlorophyll and carotenoid and decreased the proline content. Phosphorus bio-fertilizer also increased catalase enzyme activity in the plant. The effects of supplementary irrigation and phosphorus bio-fertilizer on the number of umbrella per plant, plant height, 1000-seed weight and the number of seeds per umbrella were significant. The highest amount of 1000-seed weight (2.55 g), seed yield (622.4 kg ha-1), biological yield (1252 kg ha-1), and essential oil percentage (54.86%) was obtained in the mycorrhiza treatment together with the phosphorus bio-fertilizer. In general, the results showed that using phosphorus bio-fertilizer, mycorrhiza, and supplementary irrigation can improve physiological traits, yield, and essential oil of cumin under rainfed conditions.
M. Salmalian; A. Ghasemnejad; K. Mashayekhi
Abstract
Using stimulants is an efficient way to increase the production of secondary metabolites of medicinal plants under in vivo and in vitro conditions. The present study aimed to investigate the effect of NaCl (0 and 50 mM), salicylic acid, and methyl jasmonate (each in 0 and 100 μM) in solid and liquid ...
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Using stimulants is an efficient way to increase the production of secondary metabolites of medicinal plants under in vivo and in vitro conditions. The present study aimed to investigate the effect of NaCl (0 and 50 mM), salicylic acid, and methyl jasmonate (each in 0 and 100 μM) in solid and liquid culture media on the biochemical properties and phenylalanine ammonia-lyase (PAL) activity of stevia callus under in vitro conditions. This study was conducted in a completely randomized design with a two-factor factorial arrangement in four replications. The stimuli used, culture medium and their interactions had a significant effect on the measured traits. The highest amount of phenol and flavonoid production was observed in salicylic acid 100 + salinity 50 and salinity 50 treatments in solid culture medium. Treatments including salinity 50, salicylic acid 100 + salinity 50 and methyl jasmonate 100 + salinity 50 in the solid medium increased PAL enzyme activity compared to other treatments. PAL enzyme, phenol and flavonoid were positively correlated each other. The 50 mM salinity treatment also increased proline amino acid content. Therefore, in order to improve the production of secondary compounds such as phenol, flavonoids and also PAL enzyme activity in stevia, a combination of salicylic acid (100 μM) and salinity (50 mM) is recommended under solid culture medium conditions.
B. Esmaielpour; H. Fatemi; M. Moradi
Abstract
Drought stress, as one of the most important abiotic stresses, plays an important role in decreasing the yield of medicinal plants. In order to investigate the effect of nitric oxide on morphophysiological and biochemical characteristics of basil (Ocimum basilicum L.), the native population "Shahre-ray", ...
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Drought stress, as one of the most important abiotic stresses, plays an important role in decreasing the yield of medicinal plants. In order to investigate the effect of nitric oxide on morphophysiological and biochemical characteristics of basil (Ocimum basilicum L.), the native population "Shahre-ray", a factorial experiment was conducted in a completely randomized design under drought stress conditions with three replications in the research greenhouse of Mohaghegh Ardabili University in 2017. Experimental factors included drought stress at three levels of complete irrigation (drip irrigation at three-day intervals throughout the growing season) and complete stop of irrigation at early flowering stage (60 days after planting at 10-12 leaf stage) and 50% flowering (75 days after planting at 14-15 leaf stage) and nitric oxide spray with sodium nitroprusside at four concentration levels of zero (leaf spray with distilled water), 0.5, 1, and 2 mM. The foliar application was carried out from the eight-leaf stage of the plant, with intervals of two weeks to four times. Results showed that stopping irrigation at both mentioned growth stages significantly reduced plant height, plant dry weight, chlorophylls a and b, total chlorophyll, carotenoid, and relative water content, and significantly increased electrolyte leakage rate, leaf proline content, and essential oil percentage. Foliar application of 2 mM nitric oxide significantly increased plant growth under irrigation interruption, especially at 50% flowering. Increasing the concentration of nitric oxide produced the highest essential oil percentage (1.4%) at the early flowering stage under irrigation interruption conditions and significantly increased the content of essential oil components including methyl chavicol, linalool, geranial, geraniol, α-pinene, β-pinene, and myrcene. In general, the results showed that foliar application of nitric oxide at 50% flowering stage under irrigation interruption improved the growth of basil and increased the percentage and components of essential oil via osmotic adjustment, preventing chlorophyll degradation and maintenance of leaf water content.
A. Mazarie; S.M. Mousavi-nik; A. Ghanbari; L. Fahmideh
Abstract
Water deficit stress is a major environmental limiting factor for plant growth and crop productivity. In present investigation, to study the effects of jasmonic acid on reducing the damage caused by drought on growth parameters, some physiological and antioxidant responses on Salvia officinalis L., a ...
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Water deficit stress is a major environmental limiting factor for plant growth and crop productivity. In present investigation, to study the effects of jasmonic acid on reducing the damage caused by drought on growth parameters, some physiological and antioxidant responses on Salvia officinalis L., a factorial experiment in a completely randomized design with three replications was conducted at the University of Zabol. The experimental treatments included drought stress (irrigated in field capacity, depletion of soil water content up to 50% and 75% of FC condition) and foliar jasmonic acid spray (0, 75 and 150 mg.l-1). The results showed that spraying with a concentration of 150 (mg. l-1) jasmonic acid under drought stress increased the height (% 10.02), fresh and dry weight (27.88% and 12.81%), relative water content (21.04%) and chlorophyll a and b content (12.15% and 10.34%). The highest phenolic content (34.13 mg-1fw), proline concentration (5.52 mg-1fw), essential oil yield (2.1 mg-1fw) and the activities of peroxidase (3.15 mg-1fw) and guaiacol peroxidase (0.76 mg-1fw) were obtained in plants sprayed with 150mg.l-1 jasmonic acidand in depletion of soil water content up to 75% of FC. On the other hand, the highest ascorbate peroxidase (0.76 mg-1fw), superoxide dismutase (5.64 mg-1fw) and catalase (5.12 mg-1fw), were obtained in plants sprayed with 150 mg -1 jasmonic acid under drought stress (depletion of soil water content up to 75 and 50% FC). Based on our results, it seemed that Jasmonic acid, with increasing antioxidant defense and accumulation of osmotic regulators (such as proline), reduced the oxidative stress in plants under drought stress. Moreover, maintaining the relative water content and photosynthetic pigment improved growth conditions of sage plants in drought conditions.
Gh. Salimi; M. Feizian; N. Aliasgharzad
Abstract
Drought stress causes reduction in growth and changes in biochemical indices of plants. In order to investigate the effect of arbuscular mycorrhizal fungi and selenium on reduction the detrimental effects of drought stress in Moldavian balm (Dracocephalum moldavica L.), a split plot-factorial experiment ...
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Drought stress causes reduction in growth and changes in biochemical indices of plants. In order to investigate the effect of arbuscular mycorrhizal fungi and selenium on reduction the detrimental effects of drought stress in Moldavian balm (Dracocephalum moldavica L.), a split plot-factorial experiment based on complete randomized block design with four replications was carried out under greenhouse condition. The main factor was three levels of drought [control, 0.75 Maximum Allowable Depletion (MAD) and 0.5 MAD] and sub factors included selenium at three levels (0, 100 μg per plant and 200 μg per plant) and inoculation with mycorrhizal fungi in two levels (non-inoculated as control and Glomus.verciforme). The results indicated that drought stress reduced height, dry weight and leaf area of plants. The lowest and highest value for all of these characteristics was obtained at 0.75 MAD and without drought stress, respectively. In non-stress condition, height, dry weight and leaf area of plants obtained from inoculation with mycorrhizal fungus and selenium were significantly higher than those of control. In all levels of drought stress, the content of catalase and peroxidase enzymes in plants inoculated with mycorrhizal fungus and selenium was significantly higher than that of control. The lowest content of catalase enzyme (25.06) was obtained in control and lowest content of peroxidase (14.81) was in the treatment with mycorrhizal fungus and selenium under 0.75 MAD drought stress. The highest content of catalase enzyme (53.62) was in inoculation with mycorrhizal fungus and 200μg selenium without stress, and the highest content of peroxidase (31.59) was obtained in 0.5 MAD stress and inoculation with mycorrhizal fungus and 200 μg selenium. Also, in condition of inoculation with fungus and selenium, the content of proline and MDA increased about 1 to 1.5 folds and about 1.3 to 1.5 folds, respectively, more than the control. Results showed that the presence of mycorrhiza and selenium in drought stress condition had a positive effect on growth and biochemical indices studied.
A. Mazaraei; A.R. Sirousmehr; Z. Babaei
Abstract
Drought stress, temporally or permanently, is a more limiting factor in growth and distribution of vegetation cover than other environmental factors. In order to study the effects of vesicular arbuscular mycorrhizal and drought stress on growth and yield of Milk thistle (Silybum marianum (L.) Gaertn.), a ...
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Drought stress, temporally or permanently, is a more limiting factor in growth and distribution of vegetation cover than other environmental factors. In order to study the effects of vesicular arbuscular mycorrhizal and drought stress on growth and yield of Milk thistle (Silybum marianum (L.) Gaertn.), a pot experiment was performed as factorial based on completely randomized design in three replications in the research greenhouse of Chahnimeh at the University of Zabol. Drought stress consisted of two levels including %60 and %30 of field capacity and full irrigation as control treatment. Three mycorrhizal fungi including Glomus mosseae, Glomus versiformis, and Glomus intraradices were investigated. The results showed that after starting of drought stress, vegetative characteristics such as number of capituls, number of seeds in capitul, 1000 seeds weight, leaf number and area, root length, number of minor branches, plant height, and dry weight of leaf, stem and root, significantly decreased with increasing drought stress. RWC of leaf was intensely affected by drought and decreased from 77.3 in control treatment to 57.01 in 30% of field capacity irrigation treatment. In addition, with increasing drought stress, the amount of phosphorus in leaves decreased and the amount of potassium increased. Osmotic adjustment in Milk thistle was increased in response to drought stress and leaf proline content increased (the highest 0.13 mg/g tissue in 30% of field capacity treatment); however, silimarin content decreased from 16.35 in full irrigation to 10.24 in 30% of field capacity treatment. Inoculation with VAM significantly increased vegetative indices, silimarin content (the highest in G. mosseae application and the lowest in control), plant RWC, and leaf content of P and K under drought stress conditions compared to uninoculated plants; however, the leaf proline content was low. In general, application of mycorrhizae fungi increased drought stress resistance in Milk thistle.
M. Ebrahimi; Gh.R. Zamani; Z. Alizadeh
Abstract
As an important medicinal plant in some industries such as pharmaceutical industry, the antioxidant capacity of pot marigold (Calendula officinalis L.) in conferring drought stress, as well as physiological and yield-related traits were studied. A complete randomized block design with three replications ...
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As an important medicinal plant in some industries such as pharmaceutical industry, the antioxidant capacity of pot marigold (Calendula officinalis L.) in conferring drought stress, as well as physiological and yield-related traits were studied. A complete randomized block design with three replications was carried out in the research greenhouse of Birjand University during 2014, 2015. Four levels of drought stress (including 80, 60, 40 and 20 percent of the available soil water content) and two plant types (medicinal and ornamental) were considered in this study. According to the results, with increasing drought severity, APX activity decreased and SOD and CAT activity initially increased and then decreased. Over accumulation of reactive oxygen species along with inefficiency of the antioxidant system had possibly resulted in the impaired enzymatic antioxidant efficiency in the highest level of drought stress. Proline content increased along with increasing drought intensity, so that it was up to fourfold in the highest level of drought stress, compared to non-stressed control. Although proline is an antioxidant compound, no relationship was found between proline accumulation with antioxidant enzymes. Carotenoids, chlorophyll content and chlorophyll index all decrease, and MDA content increased with increasing drought intensity, as a result of damages to chloroplast membranes. Reduced photochemical efficiency of photosystem II was another consequence of intensified water deficit. Drought stress also negatively affected yield related traits, so that dry weight (27%), height (32%), number of lateral branches (33%) and flowers (50%) and flower yield (60%) decreased. We also found that medicinal pot marigold (824.3 kg.ha-1) produced more flower than ornamental one (654.9 kg.ha-1). In conclusion, we found that enzymatic antioxidant system of pot marigold conferred a suitable ability to reduce adverse effects of drought-induced oxidative stress. Hence, increasing pot marigold’s antioxidant activity could result in increasing its physiological resistant to drought stress and consequently improves its yield components performance.
F. Pirjalili; H. Omidi
Abstract
This research was aimed to investigate the effects of drought stress on grain yield and qualitative characteristics of three populations of Lallemantia royleana Benth. The study was conducted in the research farm of Collage of Agriculture of Shahed University. This study was conducted in a split plot ...
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This research was aimed to investigate the effects of drought stress on grain yield and qualitative characteristics of three populations of Lallemantia royleana Benth. The study was conducted in the research farm of Collage of Agriculture of Shahed University. This study was conducted in a split plot based on randomized complete block design (RCBD) with three replications during 2013-2014. The experimental factors consisted of four levels of drought stress as the main plots including control or soil water potential (SWP) of -0.5atm as field capacity (FC), soil water potential (SWP) of -3.5 atm as moderate stress, soil water potential (SWP) of -6.5 atm as relatively sever stress, and soil water potential (SWP) of -9 atm as severe stress. The Balangu populations including Mashhad, Kerman, and Taleghan were considered as sub-plots. The traits measured included grain yield, total chlorophyll, chlorophyll a, chlorophyll b, carotenoids, mucilage percent, turgor factor, and proline content. The results showed that the population and the interaction of drought stress and population had significant effects on chlorophyll a, total chlorophyll, proline and grain yield at the 1% level. The effect of drought stress was found to be significant on grain yield, photosynthetic pigments, leaf proline content (P<0.01), and the total chlorophyll content (P<5% ). However, the effect of drought stress on the content of chlorophyll b was not significant. In the severe stress conditions, the greatest change in proline content was found in Mashhad population and the relatively high stress level (26.26) as compared to control (0.7). As well, the content of chlorophyll a (0.5 mg per gram) and carotenoid decreased in moderate stress (0.96 mg/g) as compared with control treatment. Among the study populations in the control treatment (field capacity), the highest grain yield (940.78kg per hectare) was obtained from Mashhad population and the lowest grain yield (194.43 kg per ha) in the severe stress conditions (-9 atm) was obtained from Taleghan population.
A. Zand; H. Aroiee; M.R. Chaichi; S.H. Nemati
Abstract
Water scarcity adversely affects many physiological and metabolic processes of the plants. To study the effects of drought stress and fertilization on some physiological characteristics, essential oil percentage and yield of spearmint, a field experiment was conducted at the Research Farm of the College ...
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Water scarcity adversely affects many physiological and metabolic processes of the plants. To study the effects of drought stress and fertilization on some physiological characteristics, essential oil percentage and yield of spearmint, a field experiment was conducted at the Research Farm of the College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. The experiment was conducted in a split plot arrangement based on a randomized complete block design with three replications. Treatments included three irrigation treatments (100%, 75% and 50% of plant water requirement) as the main plot and four fertilizer regimes including no fertilizer (control), chemical fertilizer (N+P), bio-fertilizer (rhizome inoculation with Azotobacter, Pseudomonas and mycorrhiza) and integration of biological fertilizer and 50% chemical fertilizer as the sub-plot. The results showed that all traits measured were significantly affected by the main and interaction effects of deficit irrigation and fertilizer. The content of chlorophyll a, chlorophyll b, carotenoids, total chlorophyll, percentage and yield of essential oil and yield decreased with increasing water stress while the rate of carbohydrate and proline as well as the electrolyte leakage percentage increased. Furthermore, the results showed that under 100% and 75% irrigation systems, application of chemical and integrated fertilizers improved the physiological characteristics, yield and percentage and yield of essential oil. Under 50% irrigation regime, the best performance was related to bio fertilizer.
A Salimi; V. Rowshan; E. Khanpoor
Abstract
This research was aimed to investigate the effect of salinity on quality and quantity of essential oil components and antioxidant activity in Achillea millefolium L. The study was conducted in a completely randomized design in greenhouse, controlled environment, with four treatments including 0 (control), ...
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This research was aimed to investigate the effect of salinity on quality and quantity of essential oil components and antioxidant activity in Achillea millefolium L. The study was conducted in a completely randomized design in greenhouse, controlled environment, with four treatments including 0 (control), 50, 100 and 150 mM NaCl and four replications per treatment. The essential oil was first extracted and the active components of the essential oil were separated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) methods. Then, the proline content in fresh leaf and antioxidant activity of methanolic extract were determined. According to the results, the essential oil yield showed a significant increase at 150 mM NaCl. Regarding the essential oil composition, the main compounds were delta-cadinol, trans-beta-farnesene, α-Bisabolol, borneol, caryophyllene oxide, and menthone, showing an increase with increasing salinity. Some compounds such as sabinene, cis-β-ocimene, cis-γ-bisabolene were only detected in control treatment. Menthone, beta-bisabolene, epi-beta-santalene, and 1,8-cineole were only detected in the plants treated with salt. Proline content was increased with increased concentration of NaCl. The results showed that radical scavenging activity and reducing power of yarrow extract increased with increasing of salinity concentration. Our findings suggest that salt stress increases the antioxidant compounds in Achillea millefolium.
D. Rasouli; B. Fakheri
Abstract
In order to evaluate the effects of drought stress on quantity and quality of yield, physiological characteristics, and essential oil of Ocimum basilicum L. and Ocimum americanum L., a factorial experiment was carried out in a randomized complete block design with three replications in greenhouse condition. ...
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In order to evaluate the effects of drought stress on quantity and quality of yield, physiological characteristics, and essential oil of Ocimum basilicum L. and Ocimum americanum L., a factorial experiment was carried out in a randomized complete block design with three replications in greenhouse condition. Drought stress treatments consisted of control (without drought stress), 80, 60, and 40% of field capacity on two species of Ocimum. The traits measured were fresh and dry weights, essential oil, essential oil performance, carbohydrates, proline, nitrogen, protein, phosphorus and potassium contents. The results indicated that with increasing levels of drought stress from control to 40 percent of field capacity, the content of essential oil, carbohydrates, and proline were increased (28, 46, and 50%) in both species. However, the content of fresh and dry weight, essential performance, nitrogen, protein, phosphorus and potassium were reduced (57, 57.8, 44, 19, 16, 39 and 23%). In O. americanum, the content of fresh and dry weight, carbohydrate, proline, nitrogen, phosphorus, and protein were significantly increased under drought stress; while in O. basilicum, only essential oil content showed higher values significantly. It was observed that the majority of growth factors had the highest performance under low stress (80% of field capacity). Essential oil content, on the other hand, increased significantly under high stress condition, so that in O. basilicum, the highest essential oil content was obtained at 60 percent of field capacity. Our findings indicated that the plant performance and essential oil content were increased under average and moderately severe stress conditions, respectively.
M. Askary; F. Amini; L. Hosseinpour
Abstract
Salinity stress is one of the major abiotic stresses that have destructive effects on plant productivity and quality. An experiment was conducted to investigate the effects of zinc as alleviating agent under salinity stress on antioxidative system, proteins, proline and growth of Catharanthus roseus ...
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Salinity stress is one of the major abiotic stresses that have destructive effects on plant productivity and quality. An experiment was conducted to investigate the effects of zinc as alleviating agent under salinity stress on antioxidative system, proteins, proline and growth of Catharanthus roseus (L.) G. Don. at Arak University in 2012 autumn. A number of 49-days-old plants were treated with different concentrations of NaCl (0, 35, 70 and 100mM) alone and in combination with various ZnSO4 concentrations (0, 5 and 10μm) for 21 days. Then, the inhibition percent of 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), activity of antioxidant enzymes such as superoxide dismutase, catalase and guaiacol peroxidase, proline and protein content and growth parameters were measured. Results showed that zinc application improved shoot length, root depth, root and shoot fresh and dry weight under all salinity treatments. As a result of salinity stress, the inhibition percent of DPPH radical and superoxide dismutase, catalase, guaiacol peroxidase activity and also proline content increased 348.5%, 475.6%, 172.7%, 200% and 364%, respectively but protein content was reduced 33%. Zinc application improved protein content up to 16% in plants under salinity treatment and also reduced the proline content up to 36%. The activity of antioxidant enzymes increased significantly in treated plants with NaCl+Zn as compared with those treated with NaCl or Zn alone. These results support the positive effects of Zn application on antioxidant defense system in vinca under salinity. Zinc may act as a scavenger of reactive oxygen species for mitigating the injury on biomembranes under salt stress.
D. Rasouli; M. Solouki; B. Fakheri; S. Esmaelzadeh Bahabadi
Abstract
This study was aimed to investigate the effects of manganese stress and salicylic acid treatments at three different times on activities of antioxidant enzymes, proline, soluble sugars, photosynthetic pigments and essential oils of Mentha piperita L. The research was conducted in a greenhouse at ...
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This study was aimed to investigate the effects of manganese stress and salicylic acid treatments at three different times on activities of antioxidant enzymes, proline, soluble sugars, photosynthetic pigments and essential oils of Mentha piperita L. The research was conducted in a greenhouse at the University of Zabol in 2012-13. A factorial experiment was performed in a randomized complete block design with three replications. Plant samples were harvested at three times in 1, 3 and 5 days after spraying with Mn stress (500µM) and salicylic acid (1mM). The results indicated that manganese stress significantly affected APX and proline content of peppermint leaves during all three times. The content of proline and soluble sugars significantly increased in three times compared to the control treatment under salicylic acid and manganese stress. On the other hand, the Mn stress did not significantly decrease the photosynthetic pigments. In addition, essential oils increased with manganese stress and salicylic acid. This increase was much under salicylic acid treatment. According to the obtained results, increased APX enzyme and proline content were the physiological responses of peppermint against oxidative stress. It was also observed that salicylic acid acted as a powerful growth regulator and caused modification in manganese stress response in Mentha piperita.
F. Najafi; S. Mehrabian; R. Khavari-Nejad; Y. Ghorbani
Abstract
In the present study, the effects of different concentrations of NaCl (0, 15, 30, 45, 60, 75 and 100mM) were investigated on some physiological parameters and antibacterial activities of dill (Anethum graveolens L.). Ten-days-old seedlings, prepared in sterilized petri dishes, were transferred into pots ...
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In the present study, the effects of different concentrations of NaCl (0, 15, 30, 45, 60, 75 and 100mM) were investigated on some physiological parameters and antibacterial activities of dill (Anethum graveolens L.). Ten-days-old seedlings, prepared in sterilized petri dishes, were transferred into pots containing washed sand and irrigated with Hoagland nutrient solution. Pots were located in a growth chamber (at 25°C for 16h in light and 18°C for 8h in darkness) and after certain growth period, plants were harvested for analyses. For antibacterial analyses, the plants were treated throughout flowering and fruit production stages. Results showed that in all treated plants, proline content was increased and photosynthetic pigments were decreased in high concentrations of NaCl. Also, the studies on growth factors showed that in dill, these parameters were affected by NaCl. Studies on antibacterial activities showed that in most of the cases, the zone of inhibition was increased by increasing of salt concentration and the antibacterial effect of extract was significant in high concentrations. Of course, choosing the best way and solute for preparing the extract is important for reaching a noticeable antibacterial effect.
