Biotechnology
Z. Abravesh; H. Zare; M. Khoshnevis
Abstract
Sorbus persica Hedl. is an Iranian endemic slow-growing tree (fam. rosaceae) and is endangered. It is important in terms of the gene storage, environmental protection, and medicinal uses. To in vitro propagate this species by the lateral bud culture, a factorial experiment was conducted in a completely ...
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Sorbus persica Hedl. is an Iranian endemic slow-growing tree (fam. rosaceae) and is endangered. It is important in terms of the gene storage, environmental protection, and medicinal uses. To in vitro propagate this species by the lateral bud culture, a factorial experiment was conducted in a completely randomized design in three levels of culture medium and three levels of cytokinin with three replications. For shooting, the DKW, WPM, and MS culture media containing the cytokinins BAP, Kin, and 2ip, and for rooting, the complete and modified DKW, WPM, and MS culture media containing the auxins NAA and IBA separately and in a consecutive application were investigated. The results showed that the best sterilization treatment was a consecutive application of sodium hypochlorite 20% (v/v, 15 min) and mercuric chloride 0.1% (3 min) in summer. The best proliferation and longitudinal growth of shoots was observed in the MS medium containing IBA (0.01 mg l-1), Kin (0.25 mg l-1), and BAP (3 mg l-1) growth regulators. Also, the best rooting was obtained in the DKW medium with a quarter of the concentration of macroelements containing the NAA+IBA hormones (0.3+0.3 mg l-1).
E. Zolfeghari; E. Adeli; V. Mozafarian; S. Babaiy; Gh. Habibi Bibalan
Abstract
Native and endemic plants are considered as genetic reserves of every habitat and due to favorable morphological traits, resistance to pests and diseases and adaptation to ecological conditions are of utmost importance. The aim of the current research was to identify medicinal plants from Arasbaran flora, ...
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Native and endemic plants are considered as genetic reserves of every habitat and due to favorable morphological traits, resistance to pests and diseases and adaptation to ecological conditions are of utmost importance. The aim of the current research was to identify medicinal plants from Arasbaran flora, Mardanaghomchay watershed and ethnobotanical study related to medicinal plants and its determinant factors. To achieve this goal, after identifying the region's flora and separation of the medicinal plants, data were randomly collected through questionnaires as 180 samples were taken from the population over 20 years old. The results showed that 30% of the region's flora included valuable medicinal plants belonging to 56 genera and 30 families, among them Labiatae, Compositae, Rosacea, Urticaceae, Umbellifereae and Grossulariaceae respectively were more harvested by local people for medicinal purposes. Data analysis of the questionnaires in SPSS software with coefficient correlation of Spearman and Kruskal-Wallis test revealed that among the effective factors on identification of medicinal plants in the region, the relationship between knowledge and harvesting of medicinal plants and access to rural health center for treatment was significant (P= 0.05). It means that access to the physician has been effective on knowledge of the local people with medicinal plants and its relation with access of foresters to surrounding towns for cure is significant while there was no significant relation between the knowledge and rural people age, literacy level and the consumption of synthetic drugs (P= 0.05).
