Biological effects of essential oils and extracts
Hanieh Mohseni fazel; Karim Nozad namini; Hossein Hoseini; H. Zeinali
Abstract
Background and Objectives: Pigweed (Amaranthus retroflexus L.) is one of the most widespread weeds in the world, and regarding the amount of damage caused to crops, it is the third dominant dicotyledonous weed in the world. It is recommended to reduce the use of chemical herbicides ...
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Background and Objectives: Pigweed (Amaranthus retroflexus L.) is one of the most widespread weeds in the world, and regarding the amount of damage caused to crops, it is the third dominant dicotyledonous weed in the world. It is recommended to reduce the use of chemical herbicides through the replacement of non-chemical methods in sustainable agricultural management, and the use of plants' allelopathic effects is one of these methods. On the other hand, plants in different growth stages have different allelopathy sensitivity.Methodology: To evaluate the allelopathic effect of aqueous and alcoholic extracts of eucalyptus (Eucalyptus camaldulensis Dehnh) on Pigweed (Amaranthus retroflexus) growth stages, an experiment was carried out as a factorial based on a randomized complete block design with three replications in the greenhouse of Kashan Barij Essential Company, Iran. Treatments included aqueous and alcoholic extracts of eucalyptus (50, 75, and 100 %), controlled (0.002 concentration of 2,4-Dichlorophenoxyacetic acid, Ethanol 70%, distilled water), and three growth stages (five-leaf stage, seven-leaf stage, and stem elongation). The studied traits were measured in all three growth stages one week after treatments. To measure traits, ten plants were randomly selected from each pot. The studied traits included leaf length and width, total leaf wet weight, total leaf dry weight, stem wet weight, stem dry weight, stem length, root length, root wet weight, root dry weight, total plant weight, and shoot/root ratio. A ruler was used to determine the length and width of the leaf, the length of the root, and the length of the stem. Analysis of the variance of the data was done by MSTAC software. If the experimental factor was significant, the LSD test was used to compare the means.Results: Leaf width, total leaf fresh weight, total leaf dry weight, stem fresh and dry weight, stem length, root length, root fresh and dry weight, total plant weight, and shoot/root ratio were affected by the growth stage where the extracts were used. The effect of the concentration of aqueous and ethanol extracts of eucalyptus on leaf length and width, total leaf fresh weight, total leaf dry weight, stem fresh weight, stem dry weight, stem length, root length, root fresh weight, root dry weight, total dry plant weight, and shoot/root ratio was significant. Spraying at the stem and 5-leaf stages had the highest and lowest leaf width, stem length, and root dry weight, respectively. The extract application at the stem elongation and five-leaf stage had the highest and lowest leaf width, stem length, and root dry weight, respectively. The application of distilled water in the stem elongation phase produced the maximum leaf length (3.06 cm), total fresh and dry weight of plant leaf (1.00 and 0.2764 gr), stem fresh and dry weight (1.35 and 0.393 gr), and total fresh and dry plant weight (2.80 and 0.801 gr), respectively. The application of 75% concentration of eucalyptus ethanol extract at the 5-leaf stage produced the lowest total fresh and dry weight of plant leaf (0.188 and 0.0521 gr), stem fresh and dry weight (0.303 and 0.046 gr) and total fresh and dry plant weight (0.633 and 0.1157 gr), respectively.Conclusion: The aqueous and alcoholic extracts significantly decreased Pigweed growth indices, and its inhibitory effect was not significantly different in most traits with 2-4-D herbicide. The inhibitory effect of alcoholic extract on pigweed growth was greater than aqueous extract. Given that almost all eucalyptus extracts, either alcoholic or aqueous, significantly reduced Pigweed growth, it may be possible to reduce the pressure of this weed on crops by combining and using them as a bio-herbicide.
M.H. Asareh; M.M. Barazandeh; K. Jaimand
Volume 20, Issue 4 , January 2018, , Pages 469-476
Abstract
Eucalyptus porosa was collected from Dezfool (Fadak Garden) in Martch 2004.Dry leaves of it were hydrodistilled in a clevenger type apparatus for 20 min. to produce an oil at the yield of 0.57%(based on dry leaves).In order of quantitavely and qualitavely analyses, the oil was injected to GC.Among Twenty-one ...
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Eucalyptus porosa was collected from Dezfool (Fadak Garden) in Martch 2004.Dry leaves of it were hydrodistilled in a clevenger type apparatus for 20 min. to produce an oil at the yield of 0.57%(based on dry leaves).In order of quantitavely and qualitavely analyses, the oil was injected to GC.Among Twenty-one compounds which were identified in the oil, 1,8-cineole (58.6%), α–pinene(12.8%) and nopinone (3.11%) were the major constituents, respectively.
K. Jaimand; M.H. Assareh; M. Mirza; M. Nadery; Sh. Karimi; E. Parsa
Abstract
Eucalyptuses trees as the fast-growing forest species are native to the Pacific. This species were introduced to Iran more than 100 years ago and cultivated in the south, having perfect environment for them. This research was aimed to investigate seasonal fluctuations of essential oil and1,8-cineol content ...
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Eucalyptuses trees as the fast-growing forest species are native to the Pacific. This species were introduced to Iran more than 100 years ago and cultivated in the south, having perfect environment for them. This research was aimed to investigate seasonal fluctuations of essential oil and1,8-cineol content in Eucalyptus species of Fars province. The leaves of 14 Eucalyptus species were collected from Fars province in the middle of each season for two years (2007 & 2008). Afterward, essential oils were obtained by hydrodistillation method and compounds were identified by GC and GC/MS. Results showed that in each region, some of the species had the highest essential oil yield and quality; therefore due to the high essential oil content and 1,8-cineol, they could be recommended for large-scale cultivation in the region in order to extract essential oil. Studied Eucalyptus included: E. calcicultrix (Miq.) Blakely; E. camaldulensis var. camaldulensis Dehnh.; E. cornuta; E. fruticetorum; E. intertexta R.T. Baker; E. largiflorens F. Muell.; E. lesouefii; E. longicornis F. Muell.; E. oleosa F. Muell.; E. rudis Endl.; E. salmonophloia F. Muell.; E. salubaris; E. stricklandii Maiden; E. viridis. The essential oil yield of different species at different seasons differed from 1.0 to 6.8% in 2007 and 0.3 to 5.8% in 2008. According to the results, the highest oil yield in autumn (5.2%-6.4%), the highest percentage of 1,8-cineole in winter (91.4%-94%), and the best time to harvest were recorded for E. oleosa F. Muell at Shousni, Mamasani; therefore, E. oleosa F. Muell could be recommended for cultivation as the best species.
B. Torabi Sagvand; M. Naderi Hadji Bagher Kandi; L. Sadeghzadeh
Abstract
Essential oils in aromatic plants are one of the valuable classes of natural product with medicinal properties. Many species from the genus of Eucalyptus contain essential oils and these oils could be used because of their antimicrobial effects. In this research, the essential oils of ten Eucalyptus ...
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Essential oils in aromatic plants are one of the valuable classes of natural product with medicinal properties. Many species from the genus of Eucalyptus contain essential oils and these oils could be used because of their antimicrobial effects. In this research, the essential oils of ten Eucalyptus species were obtained by hydro-distillation and examined against Escheichia coli (1330) and Micrococcus loteus (1110). The Eucalyptus species were Eucalyptus gilli, E. microcarpa, E. kingesmillii, E. loxophleba, E. gongylocarpa, E. salubris, E. erythrocorys, E. salmonophloia, E. spathulata and E. flocktoniae. The essential oils were analyzed by capillary GC and GC/MS. Antimicrobial effects of essential oils were evaluated after dilution with dimethyl sulfoxide (DMSO) through agar diffusion method. The results showed that the main component of all essential oils was 1,8-cineole and α-pinene. The highest amount of 1,8-cineole (82.1%) was found in the oil of E. kingesmillii and the lowest amount (59.6%) was found in the oil of E. salubris. The results showed that all oils were effective against two bacteria. The diameters of inhibitory zones on Micrococcus loteus and Escheichia coli were 10 to 49 mm and 10 to 22 mm respectively.
N. Esfahanianfard; F. Sefidkon; Gh. Bakhshi Khaniki
Abstract
To investigate variations in the essential oil content and composition, leaves of three Eucalyptus species named E. melliodora, E. kingsmilli and E. dundasii were collected in the middle of four seasons during two years from two warm regions, Dezful and Shushtar in South of Iran. The essential oils were ...
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To investigate variations in the essential oil content and composition, leaves of three Eucalyptus species named E. melliodora, E. kingsmilli and E. dundasii were collected in the middle of four seasons during two years from two warm regions, Dezful and Shushtar in South of Iran. The essential oils were obtained by hydrodistillation and the oils were analyzed by GC and GC/MS. The maximum oil yield of E. melliodora was obtained in winter (3.1-3.9%) and spring (2.6-2.7%) from Shushtar samples, and in autumn (1.7-2.0%) from Dezful samples. The highest percentage of 1,8-cineole was found in winter and autumn in both regions. The maximum oil yield of E. kingsmilli was obtained in winter (2.8-3.1%) from Shushtar samples, and in authumn (2.7-3.2%) from Dezful samples. The highest percentage of 1, 8-cineole was found in winter and autumn in Shushtar and autumn, spring and winter in Dezful. The maximum oil yield of E. dundasii was obtained in winter (2.8-4.7%) from Shushtar samples, and in summer (0.9%) from Dezful samples. The highest percentage of 1, 8-cineole was found in spring and autumn in Shudhtar and spring in Dezful. There were also some variations in the amounts of other major and minor components. According to the results and to achieve a suitable quality and quantity of Eucalyptus essential oil, cultivation of E. melliodora in Shushtar and Dezful is recommended. For obtaining the highest oil yield and 1,8-cineole content from the mentioned Eucalyptus species, the best harvesting time for E. melliodora is spring and winter in Shushtar and autumn in Dezful. Cultivation of E. kingsmilli in Dezful is more suitable than that in Shushtar and the best harvesting time is spring and autumn in Shushtar and autumn in Dezful. Cultivation of E. dundasii is also recommended in Shushtar while not in Dezful due to its low yield and quality of essential oil. The best harvesting time for E. dundasii in Shushtar is winter (the highest oil yield) and spring (the highest 1, 8-cineole percentage).
M.M. Barazandeh
Abstract
In order to increas the yield and quality of an eucalyptus essential oil(eucalyptus globulus) the leaves of plant was collected from Research Station of Zaghmarz in Pasansd of Behshahr and was identified by the staff of botanical department of Research Institute of Forests and Rangelands.Dried and ground ...
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In order to increas the yield and quality of an eucalyptus essential oil(eucalyptus globulus) the leaves of plant was collected from Research Station of Zaghmarz in Pasansd of Behshahr and was identified by the staff of botanical department of Research Institute of Forests and Rangelands.Dried and ground leaves of plant were steam and hydro distilled and fractions were collected every fifteen minutes. Proceeding of distillation time resulted to increas the yield and reduce cineole content. In steam distillation method,the oil yield was increased from 1.6% after 5 min. to 2.42% after 180 min. whereas 1,8-cineole content was reduced from 82.2% to 74.8%, respectively. In hydrodistillation method, the oil yield was increased from 1.55% after 15 min. to 2.86% after 180 min. whereas cineole content was reduced from 92.7% to 85.6%, respectively. Fifteen compounds were identified among which 1,8-cineole (74.8%-82.2%), α-pinene (6.28-7.70%) and limonene (5.70-6.20%) in steam distillation method and 1,8-cineole (85.60-92.70%), α-pinene (2.02%-2.37%) and limonene (2.04%-3.10%) in hydrodistillation method were the major constituents, respectively.
H. Shaker; M.M. Barazandeh; M.B. Rezaei
Volume 15, Issue 1 , January 2003, , Pages 33-48
Abstract
In order to compare essential oils composition of Eucalyptus camaldulensis Dehn. from there locations, it was collected from Noor Abad (Fars province), Behshahr (Mazandaran province) and Tehran (Tehran province) in Feb. 1999 and hydrodistilled in the phytochemistry lab of Research Institute of Forests ...
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In order to compare essential oils composition of Eucalyptus camaldulensis Dehn. from there locations, it was collected from Noor Abad (Fars province), Behshahr (Mazandaran province) and Tehran (Tehran province) in Feb. 1999 and hydrodistilled in the phytochemistry lab of Research Institute of Forests and Rangelands.
The oils were produced in light- yellow colors at the yields of 060%, 0.14% and 0.50%, respectively (based on fresh weights).
The oils were analyzed by CGC-FID and GC/MS. Thirty-four compounds were identified in each one among which 1.8-cineole (48.8%, 1.0% abd 2.5%) p- cymene (16.3%, 35.5% and 23.9%) and spathulenol (3.7%, 2.7% and 15.4%) were the major constituents, respectively.
