N. Saidi; H. Saderi; E. Taghian; F. Sefidkon; I. Rasooli; R. Mohammad Salehi; P. Owlia
Abstract
Pseudomonas aeruginosa is one of the most common opportunistic bacteria in nosocomial infections, which has a significant resistance to antimicrobials. Due to the restrictions in the use of antibiotics, the tendency to replace them with natural products has increased. In this study, the antimicrobial ...
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Pseudomonas aeruginosa is one of the most common opportunistic bacteria in nosocomial infections, which has a significant resistance to antimicrobials. Due to the restrictions in the use of antibiotics, the tendency to replace them with natural products has increased. In this study, the antimicrobial effect of four species of Satureja essential oils (S. mutica, S. bachtiarica, S. rechingeri and S. khuzestanica) on virulence factors of P. aeruginosa was evaluated. The minimum inhibitory concentration of Satureja essential oils was determined by microdilution broth method against standard strains of P. aeruginosa including PAO1 and 8821M. In the following, the effect of sub-minimum inhibitory concentrations (sub-MIC) of essential oils was investigated on virulence factors of this bacterium including motility, biofilm formation and alginate, elastase, and alkaline protease production of these two strains. All four Satureja essential oils had antimicrobial effects against the standard strains of P. aeruginosa, and also sub-MIC concentrations of the essential oils significantly reduced the virulence factors production of these strains. In this study, the suitable antagonistic effects of Satureja essential oils were observed against P. aeruginosa standard strains. By further study, these essential oils can be used as an antimicrobial compound against this bacterium.
E. Taghian; N. Saidi; F. Sefidkon; H. Saderi; I. Rasooli; R. Mohammad Salehi; P. Owlia
Abstract
Treatment of Staphylococcus aureus (S. aureus) infection is a problematic subject in the world. The aim of this study was an evaluation of the antimicrobial effect of four species of Satureja essential oils on biofilm formation and hemolysin production in S. aureus. MIC of Satureja essential oils (Satureja ...
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Treatment of Staphylococcus aureus (S. aureus) infection is a problematic subject in the world. The aim of this study was an evaluation of the antimicrobial effect of four species of Satureja essential oils on biofilm formation and hemolysin production in S. aureus. MIC of Satureja essential oils (Satureja khuzistanica Jamzad, Satureja bachtiarica Bunge, Satureja rechingeri Jamzad, Satureja mutica Fisch. & C. A. Mey.) were determined by micro dilution broth method against standard strains of S. aureus ATCC 29213(MSSA) & ATCC 33591(MRSA). Sub- MICs of Satureja essential oils were used to assay biofilm formation and hemolysin production. The results showed that all essential oils had antimicrobial effect against standard strains of S. aureus. In the presence of sub- MICs of essential oils, biofilm formation and hemolysin production were significantly reduced. The results show the potent antimicrobial effects of Saturejaessential oils against S. aureus and more study is recommended to use it in controlling S. aureus infections.
A. Zarezadeh; F. Sefidkon; S.R. Tabaei Aghdaei; A. Mirhosseini; M.R. Arabzadeh; M.R. Mirjalili
Abstract
Essential oils of Satureja spp. contain valuable components, such as thymol and carvacrol. This research was carried due to evaluate essential oil quality and quantity of different accessions of Satureja species in cultivated condition., Seeds of 35 accessions of 10 Satureja species were collected from ...
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Essential oils of Satureja spp. contain valuable components, such as thymol and carvacrol. This research was carried due to evaluate essential oil quality and quantity of different accessions of Satureja species in cultivated condition., Seeds of 35 accessions of 10 Satureja species were collected from natural habitats and the seedlings were planted, using a randomized complete block design with for three replications at Medicinal Plants Research Station, Yazd, Iran. Aerial parts of plants were collected during four consecutive years, air dried in shadow and essential oils were extracted with hydrodistillation clevenger method. Essential oil compounds were identified by Gas chromatography (GC) and chromatography-mass spectroscopy (GC/MS). Analysis of variance showed significant differences at 1% between accessions for percentage of essential oil and essential oil yield per hectare. According to the results, accessions 15 (Satureja rechingeri) from Ilam province with 5.8% and 113.9 kg/ha essential oils, and 64% carvacrol and 12.2% thymol; accession 107 (S. spicigera) from Gillan province with 2.4% and 75.5 kg/ha essential oils, and 9.2% carvacrol and 43.4% thymol; accession 24 (S. rechingeri) from Ilam province with 4.2% and 63.8 kg/ha essential oils, and 75.4% carvacrol and 6.2% thymol; and SKM (S. bachtiarica ) from Yazd province with 2.6% and 51.5 kg/ha, essential oils, and 66% carvacrol and 0.5% thymol, respectively, showed the highest essential oil production in Yazd, Iran.
A. Zarezadeh; S.R. Tabaei aghdaei; A. Mirhosseini; M.R. Arabzadeh; L. Mirjani
Abstract
Satureja belongs to lamiaceae with different species throughout the world. Annual and perennial species of this medicinal plant grow naturally in Iran, of which nine species are endemic. In this research, perennial species of Iranian savory were evaluated during 2009-2013, Yazd, Iran. Accessions of different ...
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Satureja belongs to lamiaceae with different species throughout the world. Annual and perennial species of this medicinal plant grow naturally in Iran, of which nine species are endemic. In this research, perennial species of Iranian savory were evaluated during 2009-2013, Yazd, Iran. Accessions of different species were cultivated at Research Station of Medicinal Plants using a randomized complete block design. Different traits including percentage of plant establishment, plant height, plant canopy diameter, shoot fresh yield, shoot dry yield and oil content were measured. Variance analysis showed significant differences (p<0.01) among accessions for plant establishment, plant canopy diameter, plant height, essential oil percentage, shoot dry yield and leaf dry yield . Based on mean comparisons, SKM (Satureja bachtiarica) from Yazd, 107 (S. spicigera) from Gilan, 15 (S. rechingeri) from Ilam, 123-1 and 123-2 (S. mutica) from Khorasan province were superior accessions for valuable agronomic traits such as percentage of establishment, shoot yield and oil content.
M. Dadashpour; I Rasooli; F. sefidkon; E. Zaad Hosseingholi; Sh. Darvish Alipour Astaneh
Abstract
In the present study, the antimicrobial properties of Satureja sahendica Bornm. and summer savory (Satureja hortensis L.) essential oils were studied. The bacterial strains sensitive to the oils were in Candida albicans> E. coli> S. aureus> P. aeruginosa order. Minimum inhibitory and bactericidal ...
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In the present study, the antimicrobial properties of Satureja sahendica Bornm. and summer savory (Satureja hortensis L.) essential oils were studied. The bacterial strains sensitive to the oils were in Candida albicans> E. coli> S. aureus> P. aeruginosa order. Minimum inhibitory and bactericidal concentrations were observed in the range of 0.5-10mg/ml. Total phenolics of S. sahendica and the S. hortensis were 170.5±8.53 and 47.25±2.14 µg Gallic acid equivalent per mg sample. Antioxidative property of the oils was carried out using DPPH free radical scavenging and beta-carotene bleaching tests and the results were compared with the standard synthetic antioxidants. Lipid peroxidation inhibitions were comparable to or higher than the synthetic antioxidants. The concentrations from S. sahendica and S. hortensis oils required for 50% free radical scavenging (IC50) were 6.25 and 5.82 μg/ml respectively. Ferric-reducing antioxidant power (FRAP) of S. sahendica oil was greater than that of the S. hortensis oil. The superoxide anion radical scavenging activities of S. sahendica and S. hortensis oils were 55.28%±1.16 and 21.81%±2.46 at 15µg/ml oil with an IC50 of 9.4 and 101.2 µg respectively. Tyrosinase activity of one µg S. sahendica oil was 47.88±%1.33 while that of the S. hortensis oil at 15 µg level was 15.35%±2.9. In conclusion, the results from biological properties of Satureja sahendica are indicative of its potentials for food and drug industries applications.
A.A Hossienpour Ggazviniy; M.A. Alizadeh; A.A. Jafari; A.R. Valadabadi
Abstract
In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised ...
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In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised design with three replications were used. The different treatments including: cold stratification, after-ripening, physical scarification (sand paper), chemical scarification (Ethylic alcohol 70%) and control were used for dormancy breaking. The treated seeds were sown in 3 petri dishes as replications. Then, all samples were placed in a germinator with condition of 20°C and light-to-dark cycle of 16 hours light (1000 lux) to 8 hours dark. The germination characteristics including: germination percentage, speed of germination, length of root and shoot, vigour index, fresh weight and dry weight were evaluated during 18 days of the experiment. There were significant differences amoung species for all seed germination characteristics. The ecotype of Khuzistan from S. khuzistanica had more seed germination and vigour compared to the other three species. The seed characteristics specially speed of germination and vigour index of ecotypes from cold zone were affected by cold treatment more than the other treatments.
