Agriculture and horticulture
Mohammad Hadi Shojaei Baghini; Mahdi Naghizadeh
Abstract
Background and objectives: Cumin (Cuminum cyminum L.) is one of the oldest aromatic plants in the world, known medicinally as the second domesticated medicinal plant in Iran. Drought stress, as oxidative stress, affects plant biochemical, physiological, and morphological responses. One of the ways to ...
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Background and objectives: Cumin (Cuminum cyminum L.) is one of the oldest aromatic plants in the world, known medicinally as the second domesticated medicinal plant in Iran. Drought stress, as oxidative stress, affects plant biochemical, physiological, and morphological responses. One of the ways to increase plants' resistance to environmental stress is to use plant growth regulators such as brassinosteroids and melatonin. These hormones increase plant performance in both stress and non-stress conditions. Plant growth regulators, by affecting plant metabolites, stimulating phytohormone biosynthesis, enhancing nutrient absorption, improving defense mechanisms, stimulating root growth, and improving gas exchange, contribute to plants' quantitative and qualitative performance in stress and non-stress conditions. Although some research shows that the use of plant growth regulators such as brassinosteroid and melatonin, which are of natural origin and compatible with the environment, has a significant effect on reducing adverse effects caused by environmental stresses; however, the combined use of these plant hormones is less reported. Therefore, in this research, the influence of the foliar application of brassinosteroid and melatonin on some physiological traits, including amount of malondialdehyde, hydrogen peroxide, electrolyte leakage, activity of antioxidant enzymes, accumulation of carbohydrates and proline, malondialdehyde, hydrogen peroxide as well as quantitative and qualitative yield of cumin was examined under water deficit conditions.Methodology: This research was conducted in the research greenhouse of the Faculty of Agriculture, Shahid Bahonar University, Kerman, Iran. A factorial experiment in a randomized complete block design with six replications was carried out in 2022. The experimental treatments included different levels of drought stress (100 % and 50 % field capacity; F.C.), melatonin (0 and 100 μM), and brassinosteroid (0 and 0.75 μM).Results: The results showed that water deficit increased the amount of malondialdehyde, hydrogen peroxide, electrolyte leakage, activity of antioxidant enzymes (peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) and superoxide dismutase (SOD)), as well as raised the accumulation of carbohydrates and proline in cumin plants. Indeed, brassinosteroids and melatonin significantly increased antioxidant enzyme activities and the concentrations of osmolytes. It also decreased the levels of malondialdehyde, hydrogen peroxide as well and electrolyte leakage in drought-stressed plants. Drought stress resulted in reduced cumin grain yield. In contrast, under these conditions, the percentage of cumin seed essential oil was increased. Foliar application of brassinosteroid and melatonin in stress and non-stress conditions improved cumin yield.Conclusion: Overall, it was concluded that the use of brassinosteroid and melatonin increased the resistance of cumin plants against drought stress by increasing the activity of antioxidant enzymes, increasing the accumulation of compatible osmolytes such as proline and carbohydrate, and maintaining the stability of the membrane and ultimately enhancing the yield of cumin seeds. The application of melatonin and brassinosteroid also affected the quality of the product and increased the percentage of cumin essential oil; however, the positive effects of brassinosteroid and melatonin were greater than those of brassinosteroid or melatonin applied separately.
Biological effects of essential oils and extracts
F. Zafarmand Kashani; D. Khademi Shurmasti
Abstract
The environmental hazards of polymer packaging have increased the tendency to use the biodegradable composites. Due to the use limitations of synthetic antioxidants and antibiotics, the use of alternative natural products has been developed. In this study, the antioxidant and antimicrobial effects of ...
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The environmental hazards of polymer packaging have increased the tendency to use the biodegradable composites. Due to the use limitations of synthetic antioxidants and antibiotics, the use of alternative natural products has been developed. In this study, the antioxidant and antimicrobial effects of Zataria multiflora Boiss. and Cuminum cyminum L. alcoholic extracts in the bioactive coatings on the shelf life of chicken meat were evaluated and compared with propyl gallate. For this purpose, an experiment was conducted in a completely randomized design with five experimental treatments including the uncoated chicken fillets (control), fillets coated with 8% sodium caseinate, sodium caseinate-based coated enriched with thyme extract (1.5%), cumin extract (1.5%), and propyl gallate (100 ppm) and three replications for 10 days at refrigerator temperature. The results showed that the use of sodium caseinate coating reduced the number of aerobic mesophilic bacteria, psychrophilic bacteria, thiobarbituric acid index, peroxide value, and total volatile nitrogen compounds of the fillets during storage. The use of plant extracts and propyl gallate increased the antioxidant and antimicrobial efficiency of the bioactive coatings. At the end of storage period, the fillets coated with sodium caseinate- cumin extract showed the lowest amount of aerobic mesophilic and psychrophilic bacteria and the total volatile nitrogen compounds (p < /em><0.05) and the fillets coated with sodium caseinate- thyme extract showed the lowest thiobarbituric acid index and peroxide value (p < /em><0.05) with no statistically significant difference with the bioactive coatings containing propyl gallate. Therefore, the bioactive coating of sodium caseinate containing cumin and thyme extracts can be used to increase the shelf life of chicken fillets at the refrigerator storage for at least 10 days.
Improvement and breeding
N. Khaledi; A. Dehshiri; F. Hassani
Abstract
This study was aimed at investigating the effects of biopriming with a number of native isolates of Trichoderma harzianum on the germination and seed vigor indices of a native cumin (Cuminum cyminum L.) population. Also, the effects of extracellular enzymes produced by these isolates as elicitors on ...
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This study was aimed at investigating the effects of biopriming with a number of native isolates of Trichoderma harzianum on the germination and seed vigor indices of a native cumin (Cuminum cyminum L.) population. Also, the effects of extracellular enzymes produced by these isolates as elicitors on the production and accumulation of secondary metabolites in the seedlings obtained from bioprimed seeds were studied. Based on the results, all the T. harzianum isolates were able to produce the enzymes amylase, protease, cellulase, xylanase, chitinase, and lipase. The results also showed that the cumin seed biopriming significantly affected the germination and seed vigor indices and improved the quality and health of seeds and seedlings. In this study, the essential oil of seedlings obtained from the bioprimed seeds was extracted by water distillation and its chemical compounds were identified by GC and GC/MS. The results showed that the main compounds identified included β-pinene, ρ-cymene, limonene, γ-terpinene, terpinen-4-ol, α-terpineol, cuminaldehyde, and β-farnesene. Also, T. harzianum and the enzymes secreted by it as elicitors increased the expression of genes associated with the production and accumulation of secondary metabolites in cumin. This is the first report on the effects of seed biopriming with the native isolates of T. harzianum on the essential oil compounds of cumin seedlings. The findings of this research showed that the amount of extracellular enzymes secreted by the T. harzianum isolates is different and affects the production and accumulation of secondary metabolites in cumin.
S. Heydari Moghadam; N. Azimizadeh; H. Mohammadi
Abstract
Due to the limitation of using poisons in controlling storage pests and the risk of creating intoxication by chemical poisons for endotherms, the need to use poisons with lower risk such as plant compounds is felt more than before. Plant extracts and essential oils are consistent with nature and they ...
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Due to the limitation of using poisons in controlling storage pests and the risk of creating intoxication by chemical poisons for endotherms, the need to use poisons with lower risk such as plant compounds is felt more than before. Plant extracts and essential oils are consistent with nature and they could be used in foodstuff for pest control. In this study, the respiratory toxicity of essential oils of Cuminum cyminum L. and Rosmarinus officinalis L. and oral toxicity of C. cyminum extract were examined on Ephestia kuehniella (Zeller). Extraction from C. cyminum was done by the alcoholic method and essential oils of C. cyminum and R. officinalis were extracted using Clevenger apparatus. The C. cyminum extract was provided for pests through food plates, but essential oils were used by fumigation method. The experiments were performed in controlled laboratory conditions at a temperature of 27±1ºC and a relative humidity of 50±5% and a photoperiod of 14 hours of light and 10 hours of darkness. The impact of each material on the mortality percentage of larva and pupas and the length of larval period were studied and compared with each other. According to the results, the essential oil of C. cyminum showed the highest mortality percentage on the pests as compared with the essential oil of C. cyminum extract and R. officinalis. Therefore, regarding the positive impact of these combinations on pest losses, the use of this extract and essential oils could be a good alternative to control pests in food stores, silos etc.
F. Yousefi; N. Hasanzadeh
Abstract
This research was aimed to study the effects of essential oil of Lavandula (Lavandula angustifolia Mill.) flowers, fennel (Foeniculum vulgare Miller.) and cumin (Cuminum cyminum L.) seeds, and peppermint (Mentha piperita L.) shoots on the fungus Botrytis cinerea, causing strawberry gray mold. The study ...
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This research was aimed to study the effects of essential oil of Lavandula (Lavandula angustifolia Mill.) flowers, fennel (Foeniculum vulgare Miller.) and cumin (Cuminum cyminum L.) seeds, and peppermint (Mentha piperita L.) shoots on the fungus Botrytis cinerea, causing strawberry gray mold. The study was conducted in PDA medium. The results showed that the essential oils of fennel and cumin had the highest antifungal activity. The essential oil compounds were isolated and identified by GC and GC/MS. The results obtained for the fruits inoculated with a spore suspension (1×105 spores in ml) indicated that the cumin oil was more effective in controlling the fungus B. cinerea on strawberry fruits as compared with fennel oil. The cumin oil had the highest effect in controlling strawberry gray mold, placed in one statistical group with thiabendazole.
N. Raesee; S.M.A. Vakili; G. Sarhady; F. Torkynegad
Abstract
This research was aimed to investigate the effects of different levels of manure, iron and zinc fertilizers on yield, yield components and essential oil percentage of cumin (Cuminum cyminum L.) in Jiroft during 2012-2013. The study was performed as factorial in a randomized complete blocks design with ...
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This research was aimed to investigate the effects of different levels of manure, iron and zinc fertilizers on yield, yield components and essential oil percentage of cumin (Cuminum cyminum L.) in Jiroft during 2012-2013. The study was performed as factorial in a randomized complete blocks design with three factors and 18 treatments in three replications. Manure at two levels of zero and 150 ton ha-1, zinc sulfate at three levels of 0, 25, and 40 kg ha-1and iron fertilizer at three levels of 0, 2.5, and 5 kgha-1 were applied. In this study, plant responses consisted of yield, shoot dry weight, plant height, number of seeds per plant, number of umbels, and 1000-seed weight. Results showed that manure, iron and zinc fertilizers at different levels affected shoot dry weight, yield, number of seeds per plant, number of umbels, plant height, 1000-seed weight, and essential oil percentage significantly at 1% level of significance. In addition, the interaction effects of manure and iron and zinc fertilizers were significant on shoot dry weight, yield, number of seeds per plant, and 1000-seed weight. According to the obtained results, a combined treatment of manure (15 ton ha-1), zinc sulfate (25 kg ha-1) and iron fertilizer (5kg ha-1) could be recommended to enhance the yield and yield components of cumin in Jiroft.
M.S. Salamati; H. Zeinali
Abstract
Cuminum cyminum L. is one of the most important and economic medicinal plants with a considerable distribution in different regions of the country. This study was carried out in order to evaluate genetic variation and relationships among traits in 16 populations of Cuminum cyminum L. under field conditions ...
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Cuminum cyminum L. is one of the most important and economic medicinal plants with a considerable distribution in different regions of the country. This study was carried out in order to evaluate genetic variation and relationships among traits in 16 populations of Cuminum cyminum L. under field conditions using a completely randomized design with 4 replications. Morphological traits including seed yield, biological yield, plant height, plant weight, the number of umbels per plant, the number of umbelet per umbel, the number of seed per umbel and 1000-seed weight were studied. Results of analysis of variance showed significant differences for all studied traits (p< 0.01) except for number of umbelet per umbel. High values of phenotypic and genotypic coefficients of variation were recorded for most of the traits, indicating high variations for the studied traits. Seed yield varied from 0.75 g per plant in accession of Sirjan to 0.99 g in accession of Birjand. According to the results of correlation coefficients, a high and positive significant correlation was recorded between seed yield per plant and seed number per umbel, the number of umbel per plant, biological yield and plant height. Sixteen populations were classified into four groups based upon cluster analysis. Populations of cluster IV had superiority for seed number per umbel, seed yield, biological yield, and number of umbel per plant compared to other clusters. Consequently, crossing between superior genotypes of different clusters and testing their progeny through breeding and selection programs may result in production of cultivars with desirable agronomic characteristics.
M. Ghorbanli; F. Ahmadi; A. Monfared; Gh. Bakhshi Khaniki
Abstract
Ascorbate as a strong antioxidant has a considerable bio effect on growth of plants, such as increase in their tolerance against environment stresses. In this investigation, the effect of salt stress and its interaction with ascorbate on amount of proline, catalase and ascorbate peroxidase enzymes and ...
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Ascorbate as a strong antioxidant has a considerable bio effect on growth of plants, such as increase in their tolerance against environment stresses. In this investigation, the effect of salt stress and its interaction with ascorbate on amount of proline, catalase and ascorbate peroxidase enzymes and MDA in a medicinal plant of Cuminum cyminum L. was randomly studied in the green house conditions with three replications. Plants were treated by different concentrations of NaCl (0, 25, 50, 75, 100, 125 mmolar) and ascorbate (9 mmolar). In the plants treated with salt, the amount of proline, antioxidant enzymes activity and MDA were increased with increasing NaCl concentrations. The plants treated with NaCl and ascorbate at the same time in a same NaCl concentration, the amount of proline, catalase activity, ascorbate peroxidase and MDA were increased. The results indicated that the ascorbate was one of the antioxidants that caused an increase in resistance of Cuminum cyminum L. to salt stress.
A. Kalirad
Abstract
In order to investigate the effect of cultivation date on qualitative and quantitative traits of Cuminum cyminum L. under rainfed conditions, an experiment was conducted in a randomized completely blocks design with five cultivation dates as the treatments and four replications at research station of ...
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In order to investigate the effect of cultivation date on qualitative and quantitative traits of Cuminum cyminum L. under rainfed conditions, an experiment was conducted in a randomized completely blocks design with five cultivation dates as the treatments and four replications at research station of Sirch. Morphological traits and also seed production were recorded. The results showed that all studied traits of cultivation date had significant differences at level of 1% probability. There were also significant differences among all studied traits of the year except flowering date. No significant differences were recorded for the weight of the seed production in different years. According to the results of the mean comparisons, maximum values of the studied traits were recorded for the cultivation date of early February while maximum seed production and seed yield were obtained in 24th February. Therefore, regarding yield production 24th of February was identified as the best date for cultivation. Significant differences of the traits indicate the high variability of the climate conditions in Sirch Research Station.
F. Oroojalian; R. Kasra-Kermanshahi; M. Azizi; M.R. Bassami
Abstract
Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial ...
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Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial effects (MIC and MBC) of the essential oils were assessed on several food-borne pathogens, namely Staphylococcus aureus, Bacillus cereus, Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes by microdilution technique using ELISA reader. Because of the combinatory usage of Bunium persicum and Cuminum cyminum in folk medicine, the affect of essential oil on food organoleptic properties and MIC values of the plants, interaction of Bunium persicum and Cuminum cyminum essential oils were also studied by FICindex determination using modified dilution checkboard method. The results showed that there are noticeable differences between the essential oils as their constituents as concerned, while ρ-cymen and γ-terpinene detected in all essential oils in different percentage. The main components of essential oils of C. copticum were thymol (48.4%), ρ-cymene(21.8%) and γ-terpinene (21.3%). The major constituents of B. persicum were γ-terpinene (44.2%), cuminaldehyde (16.9%), γ-terpinen-7-al (10.5%), and ρ-cymen (8%) while those of C. cyminum were cuminaldehyde (30.2%), ρ-cymene(14.1%), γ-terpinene (12.8%), and safranal (9.4%). The ranges of minimum inhibitory concentrationof the oils were 0.03-0.5,0.18-3.0, and 0.37-3.0 mg/ml, respectively, for C. copticum, B. persicum and C. cyminum. Moreover, the combination of B. persicum and C. cyminum essential oils confirmed synergistic and additive activities against the pathogens. In conclusion, although the MIC of Bunium persicum and Cuminum cyminum essential oils were lower than C. copticum, but combinatory usage of these essential oils especially against gram-positive bacteria produced promissing results. So application of these essential oils is recommended in combination as an efficients and complementary method for control of food borne pathogens with lower side effects on organolpetic properties of food.
J. Zarrinzadeh; M. Mirza; H. Alyari
Volume 23, Issue 1 , May 2007, , Pages 134-140
Abstract
In order to study the effects of plantation date and irrigation regimes on quantity and quality of herbal essential oil of Cuminum cyminum factorial experiment was carried out on the basis of randomized complete block design by three repetitions in research field of Agricultural Faculty of Tabriz University ...
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In order to study the effects of plantation date and irrigation regimes on quantity and quality of herbal essential oil of Cuminum cyminum factorial experiment was carried out on the basis of randomized complete block design by three repetitions in research field of Agricultural Faculty of Tabriz University in 2002-2003. The cuminaldehyde compound which possesses the highest percent of Cuminum cyminum L. oil, was affected significantly (at 0.01 level) by planting date. However, different levels of irrigation did not have significant effect on cuminaldehyde. The p-cymen compound was in the second rank in term of percentage rate in the oil of Cuminum cyminum. Different levels of irrigation had significant effect on the amount of p-cymene (at 0.05 level).
