Improvement and breeding
J. Gheshlaghpour; R. Khademian; B. Asghari; B. Sedaghati
Abstract
To evaluate the silicon effects on morphological characteristics as well as the percentage and composition of basil (Ocimum basilicum L.) essential oil under cadmium stress, a factorial greenhouse experiment was conducted in a completely randomized design. The treatments included cadmium metal (Cd) as ...
Read More
To evaluate the silicon effects on morphological characteristics as well as the percentage and composition of basil (Ocimum basilicum L.) essential oil under cadmium stress, a factorial greenhouse experiment was conducted in a completely randomized design. The treatments included cadmium metal (Cd) as the first factor at three levels of 0, 25, and 50 mg kg-1 soil and silicon (Si) as the second factor with concentrations of 0, 1, and 2 mM. The characteristics including root length, fresh and dry weight of roots, plant height, fresh and dry weight of stems, stem diameter, number of leaves, fresh weight of leaves, number of buds, number of sub-stems, and percentage and composition of essential oil were evaluated. The results showed that Cd had negative effects on all studied morphological characteristics and significantly decreased their values compared to the control. However, Si treatment improved all these traits and with increasing the concentration of this element, a significant increase in the values of these traits was observed. Application of Si in the concentration of 2 mM caused 63.8, 81, 61, and 61% increase in the number of sub-stems, root fresh weight, stem fresh weight, and number of buds compared to the control, respectively. The amounts of five major constituents of basil essential oil including 1,8-cineole, linalool, estragole, methyl eugenol, and eugenol were affected significantly with the Cd and Si treatments. However, the interaction of these two treatments showed a significant effect only on the three compounds 1,8-cineole, linalool, and methyl eugenol. The maximum amounts of 1,8-cineole (5.06%) and methyl eugenol (6.21%) were observed in the plants which simultaneously exposed to the highest concentrations of Cd and Si. The amount of linalool was also decreased with the presence of Cd and increased with the Si treatment. The Cd treatment increased the amount of estragole by 12.8% and decreased the amount of eugenol by 25.2%. The Si treatment also increased the amount of estragole by 23.9% and decreased the amount of eugenol by 18.3%.
E. Farajzadeh Memari Tabrizi; M. Roshdi Maleki; N. Farajzadeh Memari Tabrizi; V. Ahmadzadeh
Abstract
The aim of this study was to investigate the effects of seed treatment on different strains of bacterial biofertilizers (control, Azosperilium 21, pseudomonas 168, istofyu, her bacillus and a combination of strains) and different strains of mycorrhizal fertilizer (control, Glomus mossae, G. intradices, ...
Read More
The aim of this study was to investigate the effects of seed treatment on different strains of bacterial biofertilizers (control, Azosperilium 21, pseudomonas 168, istofyu, her bacillus and a combination of strains) and different strains of mycorrhizal fertilizer (control, Glomus mossae, G. intradices, G. hoei and all three strains of mycorrhiza) on growth, essential oil yield, and physiological characteristics of basil (Ocimum basilicum L.). Experiments were carried out in 2009 as split plot based on randomized complete block design with three replications in the farms of the Malakan Islamic Azad University. The measured traits were crop height, leaf area, chlorophyll index, chlorophyll a content, chlorophyll b content, number of stomata in the leaf surface, number of stomata in the substrate, essential oil content, essential oil yield, dry matter yield, glycol peroxidase, catalase and proline. In this study, mycorrhizal fertilizer treatments had a positive effect on the growth and yield of basil essential oil. In addition to the improvement of growth indices, physiological parameters including chlorophyll a and b contents, number of stomata and antioxidant content increased as a result of application of manure. The essential oil yield increased by 16.1%, 11.9% and 17.9%, respectively, due to the application of Azospirilum 21, application of bacteria, and the combined application of bacterial biofertilizer strains. This increase was mainly due to the increase in the percentage of basil essential oil. Contrary to growth characteristics, physiological characteristics such as chlorophyll a and b content and antioxidant content were positively affected by the use of bacterial biofertilizer. According to the results of this study, the application of Azospirilium 21, application of the bacteria, and the combined application of bacterial biofertilizer strains along with each of the mycorrhizal fungi strains will increase the qualitative and quantitative properties of basil essential oil.
M. Momeni Monfared; M. Mahmoodi Sourestani; M. Zolfaghari; M. Malekzadeh
Abstract
In order to evaluate essential oil yield and composition of fifteen accessions of basil (Ocimum basilicum L.), an experiment was conducted in the experimental field of department of horticultural science of Shahid Chamran university of Ahvaz as a randomized complete block design with fifteen treatments ...
Read More
In order to evaluate essential oil yield and composition of fifteen accessions of basil (Ocimum basilicum L.), an experiment was conducted in the experimental field of department of horticultural science of Shahid Chamran university of Ahvaz as a randomized complete block design with fifteen treatments (accessions) and three replications. Accessions were green (Yazd, Canada, Ramhormoz, Jahrom, Shiraz, Shahr rey, Dashtestan and Neyshabour), purple (Jahrom, Shiraz, Ramhormoz and Dashtestan), Golkhosheai, Thyrsiflora and Goldomaghrabi. Leaf yield, essential oil content, yield and components were evaluated. Results showed that there was a significant difference among accessions (P≤0.01) for measured traits. The maximum leaf yield was recorded in Golkhosheai and Goldomaghrabi accessions (613.6 and 550.33 gr.m2). The highest essential oil content was observed in Thyrsiflora (3.16%), Golkhosheai (2.93%) and Goldomaghrabi (2.07%) accessions and the highest essential oil yield was obtained in Golkhosheai, Thyrsiflora and Goldomaghrabi accessions (17.95, 14.72 and 11.42 gr.m2, respectively). According to essential oil analysis by GC-MS, approximately 32 compounds were identified in the essential oil of various basil accessions. Main essential oil compositions were linalool (0.12-68.43%), methyl chavicol (0-55.28%), geraniol (0-33.39%), geranial (0-42.09%), limonene (0-18.03%), methyl eugenol (0.38-4.85%), beta-caryophyllane (0-4.57%) and cubenol (0-6.22%). Essential oil of purple (Jahrom, Shiraz, Ramhormoz and Dashtestan) and Thyrsiflora accessions had the highest amount of methyl chavicol. Essential oil of Golkhosheai, Goldomaghrabi and Canada accessions was rich in linalool. Essential oil of green (Yazd, Canada, Ramhormoz, Jahrom, and Neyshabour) accessions had more geraniol and geranial than other accessions. Overall, the results of this study showed that each accession had a unique characteristic that can be used in pharmaceuticals, cosmetics and food industries.
A. Afkari
Abstract
In order to investigate the effects of drought stress and nitrogen fertilizer on some physiological and biochemical characteristics of basil (Ocimum basilicum L.), a split-plot experiment was conducted based on randomized complete block design in three replications in the research farm of Islamic Azad ...
Read More
In order to investigate the effects of drought stress and nitrogen fertilizer on some physiological and biochemical characteristics of basil (Ocimum basilicum L.), a split-plot experiment was conducted based on randomized complete block design in three replications in the research farm of Islamic Azad University, ArdabilBranch, 2015. The treatments included drought stress (70, 140 and 210 mm of class A evaporation pan) and nitrogen fertilizer at three levels (0, 50 and 100 kg/hectare) respectively. The results showed that the effect of drought stress treatment was significant on the traits investigated. The interaction of drought stress × nitrogen fertilizer had a significant effect on the traits measured, such as chlorophyll a, total chlorophyll, relative water content, dry matter yield, and essential oil yield. In addition, fertilizer treatment caused a significant increase in the physiological characteristics, dry matter yield, and essential oil yield. According to the results, the maximum content of chlorophyll a (2.65 mg/g fresh weight), total chlorophyll (3.42 mg/g fresh weight), relative water content (72.02 percent), dry matter yield (2261.25 kg/hectare), and essential oil yield (7.13 kg/hectare) were obtained for the control treatment and 100 kg of nitrogen per hectare. The maximum content of carbohydrates (3.40 μg/g glucose per fresh weight) and proline (0.681 µmol/g fresh weight) was related to the severe stress treatment. The content of chlorophyll a, b, total, relative water content, carotenoid, and essential oil percent and yield decreased by increasing the drought stress, while the content of carbohydrate and proline increased. Therefore, irrigation at 70 mm evaporation and 100 kg nitrogen fertilizer per hectare could be recommended as the best treatment to achieve the highest levels of chlorophyll a, chlorophyll total, relative water content, dry matter yield, and essential oil yield of basil.
A. Fallahi; A. Hassani; F. Sefidkon
Abstract
Zinc is an essential micronutrient element and has a number of vital roles in plants. In the recent years, zinc nano-fertilizers have received considerable attention due to their increased uptake by plants as they are small in size and have high rate of penetration through plant cell membrane. To study ...
Read More
Zinc is an essential micronutrient element and has a number of vital roles in plants. In the recent years, zinc nano-fertilizers have received considerable attention due to their increased uptake by plants as they are small in size and have high rate of penetration through plant cell membrane. To study the effect of foliar application of different zinc fertilizers on yield and phytochemical characteristics of basil (Ocimum basilicum L.), a pot experiment was conducted using a factorial based on a randomized complete block design with four replications during 2014 at the experimental greenhouse of the Horticultural Sciences Department of Urmia University. The treatments were three different zinc sources (zinc sulphate, zinc chelate and nano-zinc chelate) in four concentrations (0, 1, 2 and 3g/l). The results showed that foliar application of zinc had significant effect on evaluated parameters. Fresh and dry herb yield, zinc and phenol content of leaves, essential oil content, and yield increased with zinc application and in this regard, foliar application of nano-zinc chelate was more effective than two other zinc fertilizers. The lowest and the highest evaluated parameters were observed at concentrations of 0 and 3g/l, respectively. The results of essential oil analysis by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) showed that 20 components were identified in the basil essential oil. Linalool and eugenol were the main components of essential oil and they showed little variation under zinc application. Overall, the findings of this study showed that the effect of nano-zinc chelate application (especially at a concentration of 3g/l) was more effective than zinc sulphate and zinc chelate in increasing herb yield and active substances of basil.
S. Beigi; M. Azizi; S.H. Nemati; V. Rowshan
Abstract
One of the most important limiting factors in production of medicinal plants in arid and semiarid regions of the world is water shortage or drought stress. Basil (Ocimum basilicum L. var. "Keshkeny levelu") is a valuable medicinal plant and sensitive to water stress. Therefore, the current study was ...
Read More
One of the most important limiting factors in production of medicinal plants in arid and semiarid regions of the world is water shortage or drought stress. Basil (Ocimum basilicum L. var. "Keshkeny levelu") is a valuable medicinal plant and sensitive to water stress. Therefore, the current study was aimed to increase water use efficiency in basil production by conducting a factorial experiment based on randomized complete blocks design with three replications. The treatments were two superabsorbent polymers namely Terracottem™ (industrial) and Plantago ovata mucilage (Herbal) at four concentrations (0, 0.1%, 0.2% and 0.3% w/w on the basis of soil weight) with two application methods (mixed with soil only and mixed with soil+roots). The measured traits were morphological: leaf number, leaf area, shoot to root ratio, biological: number of seeds/plant, seed weight/plant, and water use efficiency, as well as two other important characteristics such as essential oil percentage and yield at flowering time. Results showed that the simple effect of treatments and their interaction had significant and positive effects on morphological, biological and biochemical traits, measured in this experiment. The best results in vegetative and generative characteristics were obtained by application of Plantagoovata mucilage and the highest essential oil percentage and yield were recorded by using Terracottem superabsorbent. The best results in both compounds were obtained at concentrations of 0.1% and 0.2% with application method in soil+roots and soil only, respectively. In conclusion, both compounds (terracottem™ and Plantago ovata) were able to reduce the adverse effects of drought stress and improved water use efficiency in basil cultivation.
M. Aghaei; A. Hasani; R. Darvishzadeh
Abstract
Plants, used by human, contain different compounds such as polyphenols. The phenols in food diet are important because of antioxidant and probably anticancer activity. In present research, total phenolic contents (using the Folin-Ciocalteu assay) and antioxidant capacity (using the Ferric Reducing Antioxidant ...
Read More
Plants, used by human, contain different compounds such as polyphenols. The phenols in food diet are important because of antioxidant and probably anticancer activity. In present research, total phenolic contents (using the Folin-Ciocalteu assay) and antioxidant capacity (using the Ferric Reducing Antioxidant Power (FRAP)) of 31 Iranian basil landraces (Ocimum basilicum L.) were measured using spectrophotometery. Linear relationship between total phenolic contents and antioxidant capacity was investigated. The similarity among studied genotypes was calculated by City block similarity coefficient and the classification of genotypes was done by the wards clustering method. Results showed that maximum and minimum total phenolic contents belonged to the landraces of Yazd with 19.84 mg/g fresh weight and Hamedan with 1.07 mg/g fresh weight, respectively. Maximum and minimum antioxidant capacity belonged to Birjand landrace with 3.73 mmol/100g, fresh weight and Shiraz II with 0.45 mmol/100g fresh weight, respectively. Phenotypic correlation between antioxidant capacity and total phenloic content in basil landraces was 0.59. According to cluster analysis, studied landraces were categorized in 3 clusters. Sanandej III and Shiraz II landraces had maximum distance from each other. It also became clear that genetic relationships among the studied populations did not match by their geographical origin. Basil populations displayed a considerable diversity for studied traits that could be useful for germplasm management and utilization into crop improvement.
M.T Ebadi; M. Rahmati; M. Azizi; M. Hassanzadeh Khayyat; A. Dadkhah
Abstract
Drying is one of the oldest methods for preservation of agricultural products after harvest. Basil (Ocimum basilicum L.) belongs to Lamiaceae family which is used as a medicinal and spice species. This experiment was carried out to determine the effect of different drying methods on drying time, ...
Read More
Drying is one of the oldest methods for preservation of agricultural products after harvest. Basil (Ocimum basilicum L.) belongs to Lamiaceae family which is used as a medicinal and spice species. This experiment was carried out to determine the effect of different drying methods on drying time, essential oil content and composition of Basil. The experiment was conducted in a randomized complete blocks design with three replications and treatments were included two temperature levels (50 and 70 °C), six microwave powers (100, 180, 300, 450, 600 and 900 w), and two drying methods (sun and shade drying). The drying process was continued until the mass of the sample was reduced to a moisture content of about 0.10 on a dry basis or 10 % on a wet basis. Results indicated minimum and maximum drying times (4.1 minutes and 48 hours) were obtained at 900 w microwave powers and shade drying, respectively. Maximum and minimum essential oil content (1.3 and 0.3 %) were obtained by shade drying and in 450, 600 and 900 w, respectively. Microwave powers had negative effect on the main compositions of essential oil (geranial and methyl chavicol) but shade drying preserved them. According to the results, shade drying was identified as a suitable method for Basil, because essential oil content and composition were considerably maintained.
