Improvement and breeding
Najmeh Hadi; Razieh Azimi; Mahdi Yahyazadeh; Maryam Mackizadeh; S. Fekri Qomi; Simin Mohit
Abstract
Background and objectives: Chamomile (Matricaria chamomilla L.) is a valuable medicinal plant with many applications in the food, pharmaceutical, and cosmetic health industries. Chamomile's biological properties are attributed to its essential oil (EO) compounds, especially chamazulene and a-bisabolol ...
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Background and objectives: Chamomile (Matricaria chamomilla L.) is a valuable medicinal plant with many applications in the food, pharmaceutical, and cosmetic health industries. Chamomile's biological properties are attributed to its essential oil (EO) compounds, especially chamazulene and a-bisabolol oxide A, and flavonoids, esp. apigenin and luteolin. Evaluation of wild plant populations belonging to different geographical regions in situ (study on wild samples) and ex-situ (study on wild samples under agricultural conditions) is a crucial step in plant breeding and selection of promising genotypes. On the other hand, cultivation and domestication of wild plants under agricultural conditions improve plant yield and prevent unnecessary harvesting and extinction of the plant. In the present study, the quantitative and qualitative EO diversity of some wild chamomile was investigated.Methodology: Flowers and seeds of 15 wild chamomile populations were collected from Iran's natural habitats, including 12 populations from Khuzistan province (Kh1-12), 2 populations from Fars province (F1-2), and 1 population from Bushehr province (F3) in 2021 (February-May). Flowers were used for essential oil extraction, and seeds were planted in a randomized complete block design (treatment = genotype) with three replications. The research farm located at Alborz Research Station, affiliated with the Research Institute of Forests and Rangelands, Alborz province, was considered a cultivation site without adding fertilizer to the soil. The seeds were sown directly in the field with a 15 cm distance between the planting lines and 15 cm between the plants on the lines (April 2021). Drip irrigation was used, and weeding was done mechanically. Flowers with less than 5 cm of peduncles were harvested manually at the 70% full bloom stage. The shade-dried flower EOs were extracted by water distillation (Clevenger) for 3 hours, and their quantitative and qualitative analysis was done using GC and GC/MS.Results: The results showed that sesquiterpene hydrocarbons, oxygenated sesquiterpenes, and diacetylenes made the highest EO compounds percentage in both wild and cultivated samples. Chamazulene (from sesquiterpene hydrocarbons) and a-bisabolol oxide A (from oxygenated sesquiterpenes), as two important chamomile EO compounds, showed an increase from wild to cultivated samples. Among the wild samples, the highest chamazulene (5.3%) and a-bisabolol oxide A (21.5%) contents were assigned to the populations Kh8 and Kh4, respectively. In the cultivated samples, the highest amount of these compounds (11.1 and 32.3%, respectively) was obtained in the populations Kh7 and F2, respectively. In general, the main EO compounds (%) in the wild and cultivated populations included α-bisabolone oxide A (wild: 31.3 (F1) to 64.5 (Kh3) and cultivated: 29.8 (F2) to 56 (Kh3)), α-bisabolol oxide A (wild: 5.8 (Kh5) to 21.5 (Kh4) and cultivated: 10.3 (Kh3) to 32.3 (F2)), E-β-farnesene (wild: 6.1 (Kh3) to 23.3 (Kh8) and cultivated: 6.9 (Kh1) to 15.6 (F3)), Z-spiroether (wild: 0 (F1) to 16.1 (Kh1) and cultivated: 9.1 (Kh7) to 15.1 (Kh13)), and chamazulene (wild: 1.6 (F1) to 5.3 (Kh8) and cultivated: 4.7 (Kh6) to 11.1 (Kh7)). Also, the EO% was obtained more in the cultivated samples (0.9 (Kh5) to 1.4% (Kh13)) than in the wild ones (0.1 (Kh6) to 0.5% (Kh10).Conclusion: The results of this research showed that by cultivating wild populations under agricultural conditions and water and crop management, it is possible to have essential oil in the desired quantity and quality compared to wild ones. It should be noted that the results of repeating population cultivation under the same conditions in the following years will be reported in proportion to the data output.
Phytochemistry (extraction, identification and measurement of active components)
A. Mahmoudi; M. Karami; M.T. Ebadi; M. Ayyari
Abstract
To evaluate the effect of infrared drying on color parameters, essential oil content and composition of German chamomile (Matricaria chamomilla L.), three different infrared intensities (125, 132, and 220 W) with three airflow velocities (0.5, 1.0, and 1.5 m.s-1) on a total of nine treatments were ...
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To evaluate the effect of infrared drying on color parameters, essential oil content and composition of German chamomile (Matricaria chamomilla L.), three different infrared intensities (125, 132, and 220 W) with three airflow velocities (0.5, 1.0, and 1.5 m.s-1) on a total of nine treatments were examined in a completely randomized design, and the shade-dried plants were considered as a control treatment. The results indicated that the treatments had significant effects on drying time, color parameters, and essential oil content. The control (shade drying) and the 220 W radiation intensity with 0.5 m.s-1 air velocity treatments had the highest (2367 min) and lowest (83 min) drying time, respectively. With increasing infrared intensity, the main color parameters such as L, a, b, ΔE (total color changes) and BI (browning index) changed significantly, compared to the control; however, increasing the airflow rate caused less variation of the mentioned parameters in all levels of infrared radiation intensity than the control. The highest essential oil content (0.23% w/w) was obtained at 125 W radiation intensity with 0.5 m.s-1 air velocity and the lowest (0.12% w/w) was observed at the 220 W radiation intensity with 0.5 and 1 m.s-1 air velocity treatments. Essential oil analysis showed that the highest amount of α-bisabolol-oxide A (60.6%), (Z)-spiroether (16.5%), and chamazulene (4.6%) belonged to 132 W radiation intensity with 0.5 m.s-1 air velocity, 220 W radiation intensity with 0.5 m.s-1 air velocity, and control treatments, respectively. In general, the results of this experiment showed that due to the decreased duration of drying time, color preservation and desired essential oil content and composition, the 125 W radiation intensity with 0.5 m.s-1 air velocity treatment could be a suitable method for chamomile flowers drying.
A. Farhadi; M. Daneshvar; H.R. Eisvand; F. Nazarian Firoozabadi
Abstract
In order to study the effects of different amounts of humic acid fertilizer on morphological characteristics, yield components and essential oil yield of German chamomile (Matricaria chamomilla L.) under low irrigation stress, a research was carried out in the Agricultural Research Farm of Lorestan University ...
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In order to study the effects of different amounts of humic acid fertilizer on morphological characteristics, yield components and essential oil yield of German chamomile (Matricaria chamomilla L.) under low irrigation stress, a research was carried out in the Agricultural Research Farm of Lorestan University during 2016-2017. The experiment was conducted in a split plot based on randomized complete block design with three replications. The experimental factors included irrigation at three levels (irrigation after 30, 60 and 90 mm evaporation from class A evaporation pan as the main factor) and consumption of humic acid at three levels (0, 5 and 10 kg /ha as sub factor). The results showed that with increasing irrigation interval, the flower fresh weight, flower dry weight, receptacle diameter, stem height and stem diameter were significantly decreased. With increasing levels of humic acid, flower harvest index, root dry weight, biological yield, essential oil yield, chamazulene yield, chlorophyll content (a, b and total) showed significant increase at all irrigation levels. In the case of flower harvest index, there was no significant difference in irrigation after 90 mm evaporation in humic acid treatments. The highest seed yield was obtained in irrigation after 60 mm evaporation and using 5 kg/ha humic acid. The highest yield of essential oil and chamazulene was obtained in irrigation after 60 mm evaporation and using humic acid at a rate of 10 kg/ha. According to the results, irrigation after 60 mm evaporation with 10 kg of humic acid per hectare is suggested for the production of maximum essential oil and chamazulene. As well, the same irrigation level with 5 kg/ha humic acid could be recommended to produce maximum seed yield.
H. Zeinali; A. Moslehi Yazddeli; L. Safaei; Z. Jaberalansar; A. Akhondi; Z. Skanderi
Abstract
This research was aimed to investigate the effects of different amounts of NPK fertilizers on quantitative and qualitative traits of Matricaria chamomilla L. The experiment was conducted during 2007-2008 in Barij Essence Company of Kashan in a factorial experiment based on randomized complete block design ...
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This research was aimed to investigate the effects of different amounts of NPK fertilizers on quantitative and qualitative traits of Matricaria chamomilla L. The experiment was conducted during 2007-2008 in Barij Essence Company of Kashan in a factorial experiment based on randomized complete block design (RCBD) with three replications.Treatments consisted of three levels of N (0, 50, 100 kgha-1), P2O5 (0, 25, 50 kgha-1) and K2O (0, 25, 50 kgha-1). Plant height, number of flower per plant, number of lateral branches, dry and fresh weight per plant, flower yield/m2, flower diameter, essential oil percentage, and chamazulene were measured. N levels showed significant differences for the number of flower per plant, fresh weight per plant, number of lateral branches, flower yield/m2 and dry and fresh weight of flower (p < 0.05). Analysis of data showed significant differences for fresh weight per plant under different levels of P2O5 (p < 0.05). K levels showed no significant differences for all traits. Interaction of N and P2O5 levels revealed significant differences for fresh and dry weight per plant and flower yield/m2 (p < 0.01). Interaction of N and K2o5 was significant for flower diameter (p < 0.05). Analysis of mean comparison for flower yield per m2 showed that the highest flower yield was obtained at 50 kg/ha N and 25 kg/ha P2O5, and the lowest flower yield was obtained at 100 kg/ha N and 50 kg/ha P2O5. NPK fertilizers did not affect chamazulene and essential oil. In conclusion, Matricaria chamomilla L. requires few nutrient elements.
S. Sarani; M. Heidari; M. Glavi; B. Siahsar
Abstract
To evaluate the effects of salinity in the presence and absence of iron on growth, photosynthesis pigments and electrophoresis bands in two chamomiles, an experiment was conducted as completely randomized factorial design with three replicates at university of Zabol in 2009. The treatments were two genuses ...
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To evaluate the effects of salinity in the presence and absence of iron on growth, photosynthesis pigments and electrophoresis bands in two chamomiles, an experiment was conducted as completely randomized factorial design with three replicates at university of Zabol in 2009. The treatments were two genuses of chamomile (German and Roman chamomile), four levels of salinity (0, 50, 100 and 150 mM NaCl) and two levels of iron (0 and 100 μmol). Results showed that, by increasing salinity from 0 to 150 mM NaCl, fresh and dry weight of shoot were reduced but root dry weight was added. At the absence of iron treatment, fresh and dry weight of both shoot and root were reduced in two genus of chamomile. Salinity decreased the amount of two pigments chlorophyll a and b and increased the amount of carotenoids. At the absence of iron, the amount of chlorophyll ‘a’ (32/9) and chlorophyll ‘b’ (35/5) percent decreased in comparison to control treatment (100 μmol). Unlike chlorophyll, at the absence of iron treatment, the amount of carotenoid was added. At the salinity stress from 0 to 150 mM, many protein bands in Roman chamomile were excluded. However, at the levels of 50 and 100 mM NaCl, a 42 KDa fraction band in this material was synthesized, but at the highest level of salinity (150 mM NaCl) many of their bands were removed. Presumably, the 150 mM NaCl is out of tolerance of Roman chamomile genotype. In this study, at the German chamomile genotype under salinity and iron deficiency no bonds were not removed and two bands 14 and 18 KDa were appeared.
M. Ghazi Manas; Sh Banj Shafiee; M.R. Hajseyd Hadi; M.T Darzi
Abstract
The main objective of this study was to assess the effects of different levels of vermicompost and nitrogen on qualitative and quantitative yield of chamomile (Matricaria chamomilla L.). The experimental design was factorial based on Randomized Complete Blocks Design (RCBD) with three replications, conducted ...
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The main objective of this study was to assess the effects of different levels of vermicompost and nitrogen on qualitative and quantitative yield of chamomile (Matricaria chamomilla L.). The experimental design was factorial based on Randomized Complete Blocks Design (RCBD) with three replications, conducted in Research Institute of Forests and Rangelands during spring 2010. Treatments were consisted of four levels of vermicompost (5, 10, 15 and 20 tons per hectare) and nitrogen of four levels (30, 60, 90 and 120 kg per hectare). The measured traits included number of flowers per plant, diameter of flower, dry and fresh flower yield, essential oil percentage and Chamazulene content in essential oil. Results showed that vermicompost had significant effects on dry flower weight and essential oil percentage (α ≤ 0.05%). In addition, it had significant effects on dry and fresh flower yield and Chamazulene (α ≤ 0.05%). The effect of nitrogen on the number of flower (α ≤ 0.05%) and essential oil percentage (α ≤ 0.01%) was significant. The results of interaction effects showed significant differences for the number of flower, dry flower yield, dry flower weight, essential oil percentage and Chamazulene.
M. Niknejad; M.H. Lebaschy; K. Jaimand; F. Hatami
Abstract
Manure management is a major factor in cultivation of medicinal plants. Utilization of proper fertilizers due to optimizing the plant growth could have positive effects on quality and quantity indicators. In order to investigate the effects of organic and chemical fertilizers on essential oil and components ...
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Manure management is a major factor in cultivation of medicinal plants. Utilization of proper fertilizers due to optimizing the plant growth could have positive effects on quality and quantity indicators. In order to investigate the effects of organic and chemical fertilizers on essential oil and components of Matricaria chamomilla L., an experiment was conducted using complete randomized blocks design with three replications at Research Institute of Forests and Rangelands during 2009-2010. Treatments were five fertilizers; chemical fertilizer (Ammonium phosphate) 150 Kg/ha, organic mixture (Bovine) 12 tons/ha, and chemical fertilizer 75 kg/ha, vermicompost (derived from wood waste) 7500 kg/ha, compost (plant debris) 24 tons/ha and control (no fertilizer). Results showed that the mixture treatment (organic and chemical) had the most influence on increasing the quality and quantity of phytochemical characteristics. Maximum essential oil yield (0.37 gr) and beta-ocimene (0.43%) were obtained with mixed fertilizer bysignificant difference compared to control and other treatments. Mixed fertilizers treatment caused an increase in most of the other compounds of Matricaria chamomilla compared with other treatments.
F. Salimi; F. Shekari; M.R. Azimi; E. Zangani
Abstract
To evaluate the effect of spraying with methyl jasmonate on membrance properties, ion uptake and osmotic adjustment and flower dry weight of chamomile (Matricaria chamomilla L.) under salt stress an experiment was conducted at greenhouse with three replications. The experimental design was factorial ...
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To evaluate the effect of spraying with methyl jasmonate on membrance properties, ion uptake and osmotic adjustment and flower dry weight of chamomile (Matricaria chamomilla L.) under salt stress an experiment was conducted at greenhouse with three replications. The experimental design was factorial based on randomized complete blocks design. The studied factors were as follows: spraying with methyl jasmonate at five levels including, 0, 75, 150, 225 and 300 μM in three growth stages: 1) 3 to 4 leaves, 2) stem elongation and 3) flowering (anthesis) and salt stress applied at four levels of 2.1, 6, 10 and 14 dS/m NaCl. The results showed that spraying with methyl jasmonate and salinity stress had significant effects (p ≤ 0.05) on all measured traits. The highest cell membrane stability, proline, K+, Ca+content, ratio of K+/Na+ and flower dry weight, with the lowest content of Na+ were obtained in spraying 75 µM MeJA and 6dS/m salinity stress. High amount of the mentioned parameters at the concentration of 6 dS/m salinity could be related to the assisting role of MeJA through preventing salt entrance and preferential absorption of K+ in mild salinities compared with the control treatment. In addition, application of MeJA induced significant increase in proline content. It seems that increase in proline content leads to cell membrane stability and salt resistance.
M.K. Soltani Gerdfaramarzi; H. Omidi; H. Habibi; M.H. Lebaschy; A. Zarezadeh
Abstract
For assigning of drought stress and glycine betaine effects on yield, yield components and essential oil in German chamomile (Matricaria chamomilla L.) genotypes,a field experiment was conducted in the Field of the Agricultural Educational Center of Yazd in 2008-2009 Cropping season. The experiment was ...
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For assigning of drought stress and glycine betaine effects on yield, yield components and essential oil in German chamomile (Matricaria chamomilla L.) genotypes,a field experiment was conducted in the Field of the Agricultural Educational Center of Yazd in 2008-2009 Cropping season. The experiment was established as a randomized complete blocks design using a split plot factorial with 4 replication arrangement and three factors including drought stress at three levels (irrigation at 60, 120, 180 mm evaporation pan class A) in main plots and factorial combination of Glycine betaine (no foliar application, 2, 4 kg/ha foliar application) and cultivars (Isfahan mass and Hungary breed seed) in subplot. Plant height, plant flower number, dry flower yield and relative water content were measured. Results showed there are indirect relations between drought stresses and plant height, plant flower number, dry flower yield and relative water content. Also results showed that 2 kg/ha foliar application of Glycine betaine was better than others and there are no significant different between cultivars.
A. Roohbakhsh; G. Karimi
Abstract
Over production of uric acid by xanthine oxidase (XO) causes gout. XO inhibitors for example allopurinol are the most important available anti gout drugs. Medicinal herbs are available natural sources that may be useful for the treatment of gout. In this study the inhibitory activity of aqueous extracts ...
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Over production of uric acid by xanthine oxidase (XO) causes gout. XO inhibitors for example allopurinol are the most important available anti gout drugs. Medicinal herbs are available natural sources that may be useful for the treatment of gout. In this study the inhibitory activity of aqueous extracts of Matricaria chamomilla L., Hypericum perforatum L., Fraxinus excelsior L., Zea mays L., Trachyspermum copticum (L.) Link, Cynara scolymus L. and Hedera helix L. were measured. In these experiments, under controlled conditions xanthine turns into uric acid by XO. Uric acid absorbance was measured at 295 nm using a UV spectrophotometer. Adding allopurinol (as positive control) or aqueous extracts to the solution containing XO, can decrease uric acid production by inhibition of this enzyme. At first, XO inhibitory activity of allopurinol and reproducibility of the method was evaluated by conducting three experiments. The results showed an EC50= 0.43 μg/ml for allopurinol. Then, XO inhibitory activity of aqueous extracts at 0.1, 0.5, 1, 1.5, 2 and 3 mg/ml were measured. Matricaria chamomilla could inhibit enzyme up to 68% (P< 0.001) while maximum XO inhibitory activities of Hypericum perforatum and Cynara scolymus were 36% (P< 0.001) and 21% (P< 0.001) respectively. Other extracts did not have any significant effect on XO. Our obtained results showed that part of anti gout effects of Matricaria chamomilla, Hypericum perforatum and Cynara scolymus is due to XO inhibition.
K. Jaimand; M.B. Rezaee; F. Askari; S. Meshkizadeh
Abstract
The genus of Matricaria chamomilla L is one of the important of medicinal plants used in medicinal and cosmetics industries. In pharmacogonosy, consideration of the chemical composition and pharmacological properties of chamomile can be undertaken, to be clearly understood. Plant material were collected, ...
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The genus of Matricaria chamomilla L is one of the important of medicinal plants used in medicinal and cosmetics industries. In pharmacogonosy, consideration of the chemical composition and pharmacological properties of chamomile can be undertaken, to be clearly understood. Plant material were collected, 22 Km. Away from Kazeroon city on App. 1997, and essential oils obtained from flowerheads by two different methods (Hydrodistillation & steam distillation). The oils were analyzed by capillary GC and GC/MS. Main components for hydrodistilation method, representing 93.39% of total oil were, a- Bisabolol (56.86%), trans-trans Farnesol(15.64%), sis-b-Farnesene(7.12%), Guaiazulene(4.24%) and chamazulene(2.18%) which are 86.04% from whole the oil. Main components for steam distillation method, representing 95.61% of total oil were, a-Bisabolol (51.72%), trans-trans-Farnesol (15.33%), Guaiazulene (10.51%), cis-B-Farnesene (9.48%) and chamazulene (3.69%), which are 90.73% from whole the oil. a-Bisabolol, a major component of matricaria, is reported to have anti-inflammatory can used in our medicinal and cometics industries.
