Improvement and breeding
R. Zandi; M.H. Mirjalili; Gh. Eghlima; A. Sonboli; H. Rezadoost
Abstract
Feverfew (Tanacetum parthenium (L.) Schultz-Bip.) is a perennial medicinal plant from Asteraceae family and a rich source of sesquiterpene lactone parthenolide with anti-cancer, anti-inflammatory, anti-spasm, and especially anti-migraine properties. In this study, the aerial parts of five wild populations ...
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Feverfew (Tanacetum parthenium (L.) Schultz-Bip.) is a perennial medicinal plant from Asteraceae family and a rich source of sesquiterpene lactone parthenolide with anti-cancer, anti-inflammatory, anti-spasm, and especially anti-migraine properties. In this study, the aerial parts of five wild populations of this plant were harvested from Mazandaran (Marzanabad, Siah Bisheh, and Valiabad) and Tehran (Ahar and Gachsar) provinces at full flowering stage in 2020 to evaluate their morphological traits and parthenolide content. The results showed a significant difference at 1% probability level among the studied populations for all the traits. The highest plant height (130 cm), flower dry weight (8.60 g.plant-1), and parthenolide content (0.33 mg.g-1 dry weight) were observed in Gachsar, Siah Bisheh, and Marzanabad populations, respectively. Parthenolide content correlated with leaf length, number of lateral branches, and crown diameter negatively and with number of capitols positively and significantly at 5% probability level. Cluster analysis put the feverfew populations in two main groups. Principal coordinate analysis showed that the first three factors could explain 93.06% of the total variance. Overall, the results indicated the existence of high diversity in feverfew populations for use in breeding programs and cultivation and domestication of this species.
H. Mousavi; N. Mahdi Nezhad; B. Fakheri; M. Majdi; F. Heidari
Abstract
To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium ...
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To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium L., a factorial experiment based on randomized complete block design was conducted with three replications. The experiment took place in the greenhouse of Zabol University in Iran. At first, the total RNA from samples of leaves, and then the synthesis of cDNA were extracted. Afterward, Real Time PCR analysis was used to determine gene expression patterns. Parthenolide concentration was measured by high-performance liquid chromatography (HPLC). The analysis of the data was conducted by SAS software, and there was a significant increase in the expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes of treated plants with green-synthesis silver nanoparticles in compare with the control plants, and some plants which were treated by chemical- synthesis silver nanoparticles and nano-chelate of Iron. Furthermore, the maximum expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes were identified in groups of plants which were under the influence of drought stress, or were treated by green-synthesis silver nanoparticles. A positive relation was seen between the expressions of germacrene A synthase genes (TpGAS) and parthenolide synthase (TpPTS) genes with the amount of parthenolide itself. It can be concluded that green-synthesis silver nanoparticles and water deficit stress can cause an increase on the expressions of parthenolide and germacrene A synthase genes, and as result of that, cause an increase on parthenolide production of Tanacetum parthenium.
S. Akef; F. Bernard; H. Shaker; A.R. Ghasem poor
Abstract
In this research investigation on parthenolide production and antioxidant defence in callus culture and micropropagated plantlets of feverfew (Tanacetum parthenium (L.) Sch. Bip. Family: Asteraceae) wounding, abscisic acid (ABA) and salicylic acid (SA) treatments were done. For this purpose, seeds were ...
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In this research investigation on parthenolide production and antioxidant defence in callus culture and micropropagated plantlets of feverfew (Tanacetum parthenium (L.) Sch. Bip. Family: Asteraceae) wounding, abscisic acid (ABA) and salicylic acid (SA) treatments were done. For this purpose, seeds were cultured on MS/4 medium with the whole vitamins, and shoot explants of seedlings, with shoot tip and without shoot tip and also, were cultured on MS medium supplemented with NAA (0.54µM) and BAP (4.44µM). The growth of plantlets was computed as dry weight. For biochemical investigations, shoot tip explants were cut from two months old plantlets and treated with ABA, SA and wounding (leaves discarded) for 10 hours in MS liquid medium. Parthenolide content was measured by HPLC. Antioxidant defense was evaluated as qualitative peroxidase activity. Callus tissues were obtained from leaf explants of seedlings, in MS medium supplemented with NAA (2mg.L-1) and BAP (0.5mg.L-1). One month old calli were transferred to liquid medium with ABA, SA and DMSO treatments. After one weak, parthenolide content and peroxidase activity were measured as above. Parthenolide content of control plantlets in this investigation was 0.00863±0.00283, but SA + Wounding treatment in plantlets showed the less parthenolide content and the maximum dry weight. Parthenolide production in treatments was decreased as the growth increased. In callus tissues, parthenolide content was not different significantly between treatments, and approximately half of the plantlets. Qualitative variation in antioxidant defense was shown by electrophoretic patterns and we had an extra band in wounding treatment in micropropagated plantlets. Anionic bands were stronger than cationic bands. Electrophoretic bands had no difference in callus tissues.
