H. Fatemi; B. Esmaielpour; A.A Soltani-Toolarood; A. Nematolah Zadeh
Abstract
Silicon (Si) is the second most abundant element in the earth crust. Silicon has been shown to ameliorate the adverse effects of heavy metals on plants. This research was aimed to investigate the effects of silicon nano-fertilizer nutrition on growth and physiological characteristics of coriander (Coriandrum ...
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Silicon (Si) is the second most abundant element in the earth crust. Silicon has been shown to ameliorate the adverse effects of heavy metals on plants. This research was aimed to investigate the effects of silicon nano-fertilizer nutrition on growth and physiological characteristics of coriander (Coriandrum sativum L.) under lead stress. The study was conducted in a factorial experiment based on a completely randomized design with four replications in the research greenhouse of Mohaghegh Ardabili University during 2016. Experimental treatments included soil contamination by PbCl2 (0, 500, 1000 and 1500 mg/kg soil) and foliar spraying with silicon nano-fertilizer (0, 1.5 and 3 mM). Morphological studied traits including plant height, leaf number and area, fresh and dry weight of root and plant, root and stem diameter, physiological parameters such as photosynthetic pigments, electrolyte leakage, relative water contents, proline, carbohydrates, phenol, antioxidants were measured. Results indicated that lead stress reduced the morphological characteristics such as plant height, plant fresh and dry weight, and stem and root diameter, so that the increased content of lead led to decrease of plant dry weight, root weight, root and stem diameter, root length, plant height, leaf area and dry weight up to 57, 50, 20, 41, 15, 42 and 25%, respectively. Foliar spraying with silicon (3mM) led to improve most of morphological traits of coriander. The content of chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and carbohydrate in leaves was increased with increasing lead concentration in soil. However, silicon foliar spraying improved these traits under lead stress. Proline, phenol, flavonoid and antioxidant capacity were significantly increased with increasing concentration of lead up to 1500mg/kg. In general, it can be concluded that the use of silicon nanoparticles for coriander can reduce the negative effects of lead stress to a satisfactory level.
H. Mousavi; N. Mahdi Nezhad; B. Fakheri; M. Majdi; F. Heidari
Abstract
To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium ...
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To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium L., a factorial experiment based on randomized complete block design was conducted with three replications. The experiment took place in the greenhouse of Zabol University in Iran. At first, the total RNA from samples of leaves, and then the synthesis of cDNA were extracted. Afterward, Real Time PCR analysis was used to determine gene expression patterns. Parthenolide concentration was measured by high-performance liquid chromatography (HPLC). The analysis of the data was conducted by SAS software, and there was a significant increase in the expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes of treated plants with green-synthesis silver nanoparticles in compare with the control plants, and some plants which were treated by chemical- synthesis silver nanoparticles and nano-chelate of Iron. Furthermore, the maximum expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes were identified in groups of plants which were under the influence of drought stress, or were treated by green-synthesis silver nanoparticles. A positive relation was seen between the expressions of germacrene A synthase genes (TpGAS) and parthenolide synthase (TpPTS) genes with the amount of parthenolide itself. It can be concluded that green-synthesis silver nanoparticles and water deficit stress can cause an increase on the expressions of parthenolide and germacrene A synthase genes, and as result of that, cause an increase on parthenolide production of Tanacetum parthenium.
