Phytochemistry (extraction, identification and measurement of active components)
Pooran Golkar; Golnaz Arab; Mohammad Reza Vahabi; Hamidrez Eshghizadeh
Abstract
Background and objectives: Ammi visnaga L. is a medicinal and aromatic plant in the Apiaceae family. The essential oils of this plant have highly valuable aromatic compounds with valuable therapeutic and nutritional properties. Environmental stresses affect the quantity and quality ...
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Background and objectives: Ammi visnaga L. is a medicinal and aromatic plant in the Apiaceae family. The essential oils of this plant have highly valuable aromatic compounds with valuable therapeutic and nutritional properties. Environmental stresses affect the quantity and quality of essential oils (EOs) in medicinal plants. This study was carried out to evaluate the independent and combined effects of salinity and drought stresses on EOs (%) and their chemical constituents in different organs of A. visnaga (seeds and head branches) under field conditions.Methodology: This study was carried out in the research farm of Isfahan University of Technology located in Lavark, Najafabad, using a split-plot design with three replications. Each replicate consisted of two rows (3 m long) of A. visnaga seeds. Different levels of salinity stress and drought stress were considered as main plots and subplots, respectively. Three levels of salinity stress [S1 (0 mM NaCl), S2 (80 mM NaCl), and S3 (80 mM NaCl)] and three drought stress levels based on soil depletion rate [D1: normal 50%, D2: moderate stress 60% and D3: severe stress 85%] were applied. Applying salt and drought stresses simultaneously started at the flowering stage (10%) and continued until the full maturity stage. After the full maturity stage of the plants, seed yield was measured. Then, the EOs extraction was done based on the Clevenger method in about 4 hours from head branches and seed samples for all studied treatments. The chemical constituents were identified using Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS) techniques.Results: The independent and combined stresses of drought and salinity caused a significant increase in EOs (%) of A.visnaga. The highest EOs in the head branch (1.26%) were detected under non-drought and medium salinity stress conditions (D1S1). The lowest EOs (0.25%) were observed under non-drought and non-salinity conditions (D1S1). The highest EOs (0.25%) in seed samples were observed under D2S2 conditions (moderate drought and salinity stress), but the least amount (0.12%) was found under D1S2 conditions (moderate salinity stress) and D1S3 (severe salinity stress). The analysis of essential EOs identified 25 compounds in both seeds and head branch samples. These compounds belong to six main chemical groups. The most and lowest compounds belonged to the groups of hydrocarbon monoterpenes and oxygenated diterpenes in both branches and seeds, respectively. Cembrene was identified as the main chemical compound in seed and head branch EOs. The lowest values for cembrene were reported as 23.32 % and 26.16% in seed and head branch EOs, respectively. The highest values for cembrene were reported as 33.31% and 36.62% in seed and head branch EOs under treatment. The compound 2-methyl-2-methylbutyl ester was identified as the second most abundant compound in the head branches and seeds of A. visnaga. The highest amount of this compound was observed in seed EOs (9.38%) under mild drought and mild salinity stress (D2S2), and the highest amount of this compound was observed in head branch EOs (18.5%) under mild drought stress (D2S1) conditions.Conclusion: According to the study results, drought and salinity stress caused a quantitative and qualitative increase in A. visnaga EOs. Considering the climatic conditions of the arid and semi-arid regions of Iran and the consequent gradual salinization of soils, this plant can be considered a plant of choice in medicinal plant breeding programs, as well as goals related to the food and pharmaceutical industries.
Phytochemistry (extraction, identification and measurement of active components)
F. Askari; F. Sefidkon; M.A. Soltanipour; M. Mirza; S. Fekri Qomi
Abstract
The genus Pycnocyla belongs to the Apiaceae family and has eight perennial species in Iran. In this study, the essential oil content and chemical compounds of P. nodiflora Decne ex. Boiss. and P. flabellifolia Boiss. were investigated. The aerial parts of plants at seeding stage were collected from two ...
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The genus Pycnocyla belongs to the Apiaceae family and has eight perennial species in Iran. In this study, the essential oil content and chemical compounds of P. nodiflora Decne ex. Boiss. and P. flabellifolia Boiss. were investigated. The aerial parts of plants at seeding stage were collected from two habitats in Hormozgan province through two consecutive years and Kermanshah province in one year, respectively. Stem+leaf [SL] and seed [S] were separated and shade-dried. The essential oil was extracted by Clevenger apparatus and hydrodistillation method for three hours. Percentage and chemical composition of the essential oils were determined by GC and GC/MS. The essential oil content of P. nodiflora was obtained [SL]: 0.23 and 1.21% (two years) and [S]: 0.23% in Tangzagh habitat of Hormozgan and [SL]: 0.02 and 0.06% (two years) and [S]: 0.01% in Abmah habitat. Caryophyllene oxid (7.8-28.1%) and b-eudesmol (15.5-57.2%) were recognized as the main compounds of [SL] and [S] essentials oils of both habitats in this species. In P. flabellifolia, the essential oil content of [SL] and [S] was 0.66 and 0.76%, respectively. The main compounds in [SL] essential oil of this species were cis-β-ocimene (33.8%), trans-β-ocimene (39.6%), and terpinolene (12.9%) and in [S] essential oil were trans-β-ocimene (28%), cis-β-ocimene (31%), and terpinolene (12.6%). According to previous reports on the antimicrobial properties of trans-β-ocimene and cis-β-ocimene compounds on bacteria like Staphylococcus aureus and S. epidermidis and the predominance of these compounds in the essential oil of P. flabellifolia, studies on antimicrobial properties of this species essential oil is recommended.
Identification, introduction, ecology and ethnobotany
M. Ghadimi Joboni; M. Ghavam
Abstract
Ethnobotany is the recording of traditional unwritten information for the rational exploitation of resources and the effective protection of biodiversity and cultural information in any community. The present study aimed at recording the local's indigenous knowledge of Jubon village in Rudbar city of ...
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Ethnobotany is the recording of traditional unwritten information for the rational exploitation of resources and the effective protection of biodiversity and cultural information in any community. The present study aimed at recording the local's indigenous knowledge of Jubon village in Rudbar city of Guilan province about using medicinal and edible plants. The research data were obtained through the semi-structured interviews with 35 local experts. The descriptive statistics models such as the relative frequency of citation, use value, and cultural significance indices were used to analyze the data. The results showed that 34 plant species from 19 families were used for therapeutic and edible purposes in the study area. Plants of the families apiaceae, lamiaceae, and asteraceae with the frequencies of 19, 16, and 9%, respectively, were the most used in the region. The most important medicinal plants in the region were Malva sylvestris L., Echium amoenum Fisch. & C.A.Mey., Urtica dioica L., Eryngium planum L., and Foeniculum vulgare Mill., respectively, which had the highest cultural significance and relative frequency of citation indices among the medicinal plants of the region. The findings also indicated that the most common purpose of therapeutic use of the plants was about the gastrointestinal diseases, which were mainly used orally in cooking. In general, the people of Jubon village have a rich knowledge about the use of medicinal and edible plants, and this knowledge has existed in the region for generations.
E. Aryakia
Abstract
The family Apiaceae contains valuable commercial species including medicinal, spicy, vegetable, and ornamental species. Although there are many reports on medicinal properties and phytochemical content of commercial Apiaceae species, little information is available about other relatives of this family. ...
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The family Apiaceae contains valuable commercial species including medicinal, spicy, vegetable, and ornamental species. Although there are many reports on medicinal properties and phytochemical content of commercial Apiaceae species, little information is available about other relatives of this family. In this study, the medicinal properties including anti-acetylcholinesterase, anti-tyrosinase, and antioxidant activities along with total phenolic content (TPC) of nine Apiaceae species were assessed. Our results revealed a wide variation of medicinal properties and phytochemical content between different species. The studied plants were identified as potential sources of anti-acetylcholinesterase, anti-tyrosinase, and antioxidant properties. The highest antioxidant, anti-acetylcholinesterase and anti-tyrosinase properties were found in Conium maculatum, Prangos uloptera and Malabaila secacul, respectively. Moreover, the results showed that the TPC and consequently antioxidant properties of methanolic extract were several times more than that of ethyl acetate extract in each species. Overall, the species studied in the present research possessed favorable to extreme amounts of medicinal properties and phytochemical content that was first reported here and could be considered in the pharmaceutical, food, and agricultural industries.
M. Najafpour Navaei; M. Mirza
Abstract
Scaligeria (Apiaceae) is represented in Iran by seven species, which is located in natural habitat. In this Research the fruits and leaves of Scaligeria assyriaca Freyn & Bornm. were collected from Khorasan province.The oil constituents from fruits and leaves of this plant have been studied by GC ...
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Scaligeria (Apiaceae) is represented in Iran by seven species, which is located in natural habitat. In this Research the fruits and leaves of Scaligeria assyriaca Freyn & Bornm. were collected from Khorasan province.The oil constituents from fruits and leaves of this plant have been studied by GC and GC/MS. The major constituents in leaf oil were found to be germacrene D (21.1%), β-caryophyllene (13.4%) and α-copaene (10.2%). In the fruit oil the main components were myristicin (24.3%), germacrene D (13.7%) and elemicin (11%). β-Sesquiphellandrene and kessanwere identified ranging (10% and 8.7%) and (7.4% and 9.5%) in leaf and fruit oils, respectively.
R. Omidbaigi; K. Sadrai Menjili; F. Sefidkon
Abstract
To study the effect of sowing date on fennel CV. Soroksari, this research work was carried out on randomized complete block design with three replications. The effects of sowing dates of 6 of March, 20 of March, 4 of April, 19 of April, 5 of May, 21 of May, 5 of June and 21 of June, were studied on growth, ...
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To study the effect of sowing date on fennel CV. Soroksari, this research work was carried out on randomized complete block design with three replications. The effects of sowing dates of 6 of March, 20 of March, 4 of April, 19 of April, 5 of May, 21 of May, 5 of June and 21 of June, were studied on growth, development, seed yield and essential oil and anethole content of fennel CV. Soroksari. The results show that sowing dates hade significant effect on growth, development and quality and quantity of active substances of fennel. On the basis of the results the seeds were sown on 21 of May the seeds were germinated and developed faster. The highest plant (150.8 cm) and the largest number of branches per plant (10.1) was obtained from the plants were sown on 4 of April. There was no significant effect among 1000 seeds weight and essential oil content of different sowing dates. The largest seed yield (1864.3Kg/ha) and essential oil yield (91.3Kg/ha) was obtained from the seeds were sown on 4 of April. Anethole content were decreased from the plants were sown from 6 of March to 21 of June, the lowest amount (58.2%) was extracted from the plants were sown on 21 of June. According to the results of this resesrch trial the most suitable date for sowing of fennel cv. Soroksari is recommended 20 of March to 4 of April.
