Agriculture and horticulture
H. Shabkhiz; A. Javanmard; A. Ostadi; M.R. Morshedloo
Abstract
To determine the effects of Myco-Root biofertilizer and different irrigation levels on the content and essential oil compounds of thyme (Thymus daenensis Celak.), a factorial experiment was conducted in a completely randomized design with eight treatments and three replications in the greenhouse of Plant ...
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To determine the effects of Myco-Root biofertilizer and different irrigation levels on the content and essential oil compounds of thyme (Thymus daenensis Celak.), a factorial experiment was conducted in a completely randomized design with eight treatments and three replications in the greenhouse of Plant Production and Genetics department, Faculty of Agriculture, University of Maragheh, Iran, in 2020. The factors included drought stress at four levels of without stress (irrigation at field capacity (FC) as a control), mild (irrigation at 75% FC), moderate (irrigation at 55% FC), and severe (irrigation at 35% FC) stresses and biofertilizer at two levels of inoculation and non-inoculation with Myco-Root. The results demonstrated that the fresh and dry weight of leaves, stem dry weight, total dry weight, leaf relative water content, chlorophylls a and b and total chlorophyll content, and root colonization percentage decreased significantly with increasing the water deficit level. However, the carotenoid content and essential oil percentage showed an increasing trend with increasing drought stress. The highest carotenoid content and essential oil percentage was obtained by using Myco-Root under moderate drought stress. Thymol, carvacrol, p < /em>-cymene, 1,8-cineole, (E)-caryophyllene, borneol, γ-terpinene, and α-pinene were identified as the major constituents of essential oil in the all treatments. The highest content of thymol was obtained under mild drought stress with the Myco-Root application. Furthermore, the highest content of carvacrol was recorded under mild, moderate, and severe drought stresses, respectively with the Myco-Root application. On average, the application of Myco-Root biofertilizer increased the content of thymol, carvacrol, 1,8-cineole, E-caryophyllene, borneol, and α-pinene by 4.19, 48.28, 15.93, 16.33, 14.15, and 3.78%, respectively compared to not using this biofertilizer. Overall, the results showed that some morphological and physiological traits of thyme decreased significantly with increasing the water deficit levels, but the application of Myco-Root biofertilizer could amend drought stress and improve the growth, percentage and essential oil quality of the plant. Thus, the use of Myco-Root biofertilizer instead of chemical fertilizers could be recommended to achieve the sustainable agriculture in the thyme cultivation.
Agriculture and horticulture
M. Amani Machiani; A. Javanmard; A. Ostadi; M.R. Morshedloo; J. Chabokpour
Abstract
To evaluate the effects of mycorrhizal fungus Funneliformis mosseae application and harvest time on the quantity and quality of thyme (Thymus vulgaris L.) essential oil in the different irrigation regimes, a field experiment was carried out as a split-split plot in a randomized complete blocks design ...
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To evaluate the effects of mycorrhizal fungus Funneliformis mosseae application and harvest time on the quantity and quality of thyme (Thymus vulgaris L.) essential oil in the different irrigation regimes, a field experiment was carried out as a split-split plot in a randomized complete blocks design (RCBD) with 12 treatments and three replications at the Agriculture Faculty, University of Maragheh, Iran, in 2019. The factors and their levels included 1- the main factor: irrigation regime at three levels of irrigation after 20% (W20 as control), 50% (W50 as moderate stress), and 80% (W80 as severe stress) of maximum allowable depletion, 2- the subfactor: application and non-application of mycorrhiza, and 3- sub-sub factor: harvest time at two levels of June harvest (first harvest) and September harvest (second harvest). The results demonstrated that the highest amounts of plant height, canopy diameter, and dry matter yield of thyme were obtained in the W20 (control)+ application of mycorrhiza fungus+ first harvest treatment. In addition, the highest essential oil content was recorded in W50 followed by W80, which were 38.2 and 23.7% higher than W20 (control), respectively. Also, the application of mycorrhiza fungus increased the essential oil of thyme by 8.5% compared to the non-application of fungus. The highest and lowest content of thymol, γ-terpinene, and p < /em>-cymene were obtained in W50 and W20, respectively. Also, the application of mycorrhiza fungus increased the amount of mentioned compounds, respectively by 3.8%, 3.9%, and 7.1% compared to the non-application of fungus. Overall, it can be concluded that W50 (moderate stress) along with the application of mycorrhizal fungus F. mosseae improved the essential oil quality and quantity of thyme.
A. Javanmard; P. Karimi Karamjavan; M. Amani Machiani; A. Ostadi; S. Shahbazi Doorbash
Abstract
In order to investigate the effects of combined and separate application of macro and micro nano fertilizers with chemical fertilizers on quantitative and qualitative traits of medicinal plant dragon’s head (Lallemantia iberica (MB) Fischer & Meyer), a field experiment was performed based on ...
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In order to investigate the effects of combined and separate application of macro and micro nano fertilizers with chemical fertilizers on quantitative and qualitative traits of medicinal plant dragon’s head (Lallemantia iberica (MB) Fischer & Meyer), a field experiment was performed based on the randomized complete block design (RCBD) with seven treatments and three replications on the research farm of Maragheh University in 2017. Experimental treatments included the use of 100% conventional chemical fertilizer (urea and triple superphosphate with values of 150 and 100 kg ha-1, respectively), 100% macro nano fertilizer, 100% micro nano fertilizer, 50% macro nanofertilizer+50% micro nano fertilizer, 50% chemical fertilizer+50% macro nano fertilizer, 50% chemical fertilizer+50% micro nano fertilizer, and 50% chemical fertilizer+25% macro nanofertilizer+25% micro nano fertilizer. The results showed that the highest (73.3) and lowest (59.1) chlorophyll indices were obtained by applying 50% chemical fertilizer+25% macro nano fertilizer+25% micro nano fertilizer, and separate application of 100% micro nano fertilizer, respectively. Also, the highest values of number of lateral branches, main stem length, number of flower cycles per plant, number of seeds per plant, biological yield, seed yield, 1000-seed weight and seed mucilage content were obtained in 50% chemical fertilizer+25% macro nanofertilizer+25% micro nanofertilizer treatment. Chemical analysis of the essential oils demonstrated that linalool, menthol, geraniol, geranial, menthyl acetate, β-bourbonene, geranyl acetate, (E)-caryophyllene, germacrene D, spathulenol and caryophyllene oxide were the main constituents of essential oils. The highest content of β-bourbonene, caryophyllene oxide, (E)-caryophyllene, menthol, and geraniol was obtained with the combined use of 50% chemical fertilizer+25% macro nanofertilizer+25% micro nano fertilizer. In general, based on the results of the present research, the combined use of chemical fertilizers and macro and micro nano fertilizers can be recommended due to the increased seed yield and dragon’s head qualitative traits under rainfed conditions.