F. Nosrati; B. Fakheri; M. Solouki; N. Mahdi Nezhad; M. Valizadeh
Abstract
Sarcocolla(Astragalus fasciculifolius Boiss.), belonging to Fabaceae family, is a medicinal plant distributed in the south of Sistan and Baluchestan province. Due to presence of compounds like saponins, flavonoids, and polysaccharides, it has an effective role in treating heart disease and cancer and ...
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Sarcocolla(Astragalus fasciculifolius Boiss.), belonging to Fabaceae family, is a medicinal plant distributed in the south of Sistan and Baluchestan province. Due to presence of compounds like saponins, flavonoids, and polysaccharides, it has an effective role in treating heart disease and cancer and eliminating chemotherapy and improving immunity. In this study, plant samples including eight A. fasciculifolius were collected from different habits in the province, and some phytochemical properties of root, leaves (total phenol, total flavonoid, and polysaccharide) and of flowers (total phenol, total flavonoid, and anthocyanin) were analyzed in the plant flower. The results of variance analysis showed a significant difference among different habitats (α>0.01). Phytochemical studies revealed significant amounts of phenolic compounds in the leaf with the highest rate recorded in Poshtkooh (14.62-32.55 mg GAE/g). In the root, the highest value was in Anjirok (20.39-53.84 mg GAE/g) and in the flower, the highest value was in BAirk (5.11-15.82 mg GAE/g), representing flavonoid compounds in the leaf in which the highest value was in Nahook (8.76-12.72 mg QE/g). Also, in root, the highest value was in Panjangosht (10 fingers) (0.25-1.03 mg QE/g). In the flower, the highest value was in Nahook (6.35-9.28 mg QE/g). In the polysaccharide compounds of the leaf, the highest value was in Anjirok (60.89-93.29 mg/g). In root, the highest values were in Pangangosht (94.33-209.38 mg/g) and anthocyanin of flower had the highest values in Pangangosht (36.34-77.58 mg/g). The results of this study represent the ability of different habitats of the province in producing valuable herbal compounds. Besides, because of its high levels of phenolic and polysaccharide compounds, this plant has a remarkable capability to be used in food and pharmaceutical industries.
H. Mousavi; N. Mahdi Nezhad; B. Fakheri; M. Majdi; F. Heidari
Abstract
To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium ...
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To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium L., a factorial experiment based on randomized complete block design was conducted with three replications. The experiment took place in the greenhouse of Zabol University in Iran. At first, the total RNA from samples of leaves, and then the synthesis of cDNA were extracted. Afterward, Real Time PCR analysis was used to determine gene expression patterns. Parthenolide concentration was measured by high-performance liquid chromatography (HPLC). The analysis of the data was conducted by SAS software, and there was a significant increase in the expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes of treated plants with green-synthesis silver nanoparticles in compare with the control plants, and some plants which were treated by chemical- synthesis silver nanoparticles and nano-chelate of Iron. Furthermore, the maximum expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes were identified in groups of plants which were under the influence of drought stress, or were treated by green-synthesis silver nanoparticles. A positive relation was seen between the expressions of germacrene A synthase genes (TpGAS) and parthenolide synthase (TpPTS) genes with the amount of parthenolide itself. It can be concluded that green-synthesis silver nanoparticles and water deficit stress can cause an increase on the expressions of parthenolide and germacrene A synthase genes, and as result of that, cause an increase on parthenolide production of Tanacetum parthenium.
D. Rasouli; B. Fakheri
Abstract
In order to evaluate the effects of drought stress on quantity and quality of yield, physiological characteristics, and essential oil of Ocimum basilicum L. and Ocimum americanum L., a factorial experiment was carried out in a randomized complete block design with three replications in greenhouse condition. ...
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In order to evaluate the effects of drought stress on quantity and quality of yield, physiological characteristics, and essential oil of Ocimum basilicum L. and Ocimum americanum L., a factorial experiment was carried out in a randomized complete block design with three replications in greenhouse condition. Drought stress treatments consisted of control (without drought stress), 80, 60, and 40% of field capacity on two species of Ocimum. The traits measured were fresh and dry weights, essential oil, essential oil performance, carbohydrates, proline, nitrogen, protein, phosphorus and potassium contents. The results indicated that with increasing levels of drought stress from control to 40 percent of field capacity, the content of essential oil, carbohydrates, and proline were increased (28, 46, and 50%) in both species. However, the content of fresh and dry weight, essential performance, nitrogen, protein, phosphorus and potassium were reduced (57, 57.8, 44, 19, 16, 39 and 23%). In O. americanum, the content of fresh and dry weight, carbohydrate, proline, nitrogen, phosphorus, and protein were significantly increased under drought stress; while in O. basilicum, only essential oil content showed higher values significantly. It was observed that the majority of growth factors had the highest performance under low stress (80% of field capacity). Essential oil content, on the other hand, increased significantly under high stress condition, so that in O. basilicum, the highest essential oil content was obtained at 60 percent of field capacity. Our findings indicated that the plant performance and essential oil content were increased under average and moderately severe stress conditions, respectively.
D. Rasouli; M. Solouki; B. Fakheri; S. Esmaelzadeh Bahabadi
Abstract
This study was aimed to investigate the effects of manganese stress and salicylic acid treatments at three different times on activities of antioxidant enzymes, proline, soluble sugars, photosynthetic pigments and essential oils of Mentha piperita L. The research was conducted in a greenhouse at ...
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This study was aimed to investigate the effects of manganese stress and salicylic acid treatments at three different times on activities of antioxidant enzymes, proline, soluble sugars, photosynthetic pigments and essential oils of Mentha piperita L. The research was conducted in a greenhouse at the University of Zabol in 2012-13. A factorial experiment was performed in a randomized complete block design with three replications. Plant samples were harvested at three times in 1, 3 and 5 days after spraying with Mn stress (500µM) and salicylic acid (1mM). The results indicated that manganese stress significantly affected APX and proline content of peppermint leaves during all three times. The content of proline and soluble sugars significantly increased in three times compared to the control treatment under salicylic acid and manganese stress. On the other hand, the Mn stress did not significantly decrease the photosynthetic pigments. In addition, essential oils increased with manganese stress and salicylic acid. This increase was much under salicylic acid treatment. According to the obtained results, increased APX enzyme and proline content were the physiological responses of peppermint against oxidative stress. It was also observed that salicylic acid acted as a powerful growth regulator and caused modification in manganese stress response in Mentha piperita.
