F. Askari; E. Sharifi Ashorabadi; M. Mirza; M. Teimouri; E. Ehsani
Abstract
This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized ...
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This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized design with four replications. In this experiment, collection locality at six levels including the habitats of West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan, essential oil dilution at three levels including one fifth (1:5), one twenty-fifth (1:25), one fiftieth (1:50) and comparison with the antibiotics of ciprofloxacin and ceftizoxime, and studied microorganisms at five levels including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Candida albicans were investigated. The seeds were collected from different habitats and cultivated in the field of Research Institute of Forests and Rangelands. The essential oil was isolated from shoots by hydro-distillation. Chemical compositions of the oils were analyzed by GC and GC/MS. The essential oil yield obtained from different localities varied between 0.39% to 0.83% (w/w). The major constituents of the essential oil obtained from the samples collected from West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan were as follows: West Azarbaijan: E-caryophyllene (26.0%) and camphor (24.2%); Tehran: geranial (30.9%) and geranyl acetate (23.9%); Zanjan: linalool (23.5%) and 1,8-cineol (22.2%); Qazvin: thymol (30.3%) and carvacrol (30.1%); Kurdistan: linalool (17.2%) and geranyl acetate (12.7%), and Guilan: α–terpineol (31.2%) and geraniol (11.2%). Analysis of variance of inhibition zone diameter of T. pubescence essential oil showed significant difference (p<0.05) among collection locality, microorganism type, essential oil dilution and their interaction. Mean comparisons of the collection locality, microorganism type and essential oil dilution showed that the highest inhibition zone diameter (35.5mm) was recorded for the essential oil obtained from West Azarbaijan, ciprofloxacin against Bacillus subtilis. The lowest inhibition zone diameter (8.0 mm) was observed in the essential oil obtained from West Azerbaijan with essential oil dilution of 1:50 against P. aeruginosa. According to the MIC and MBC results, the most and the least antimicrobial activity was recorded for the essential oils obtained from the seeds collected from Tehran and Kurdistan, respectively.
Z. Baher Nik; M. Teimori; M. Mirza
Abstract
In this research, the effects of different irrigation treatments on chemical constituents and antibacterial effects of theessential oil of P. argentatum were studied. The experimental design was randomized complete block design with four replications. Four irrigation treatments were determined, ...
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In this research, the effects of different irrigation treatments on chemical constituents and antibacterial effects of theessential oil of P. argentatum were studied. The experimental design was randomized complete block design with four replications. Four irrigation treatments were determined, consisting of: (a) control, which was irrigated to full field capacity (FC); (b) low water stress treatment (LS1=75% of FC); (c) moderate water stress treatment (LS2=50% of FC); (d) sever water stress (HS=25% of FC). Then the volatile constituents of the flowers were isolated and their quantities, qualities and antibacterial effect determined against four gram negative and four gram positive bacteria on the basis of disc-diffusion method. The result showed that not only the quantity of the essential oils but also the qualities varied. The percentage of α-pinene, β-pinene, γ-eudesmol and β-eudesmol has changed. The LS2 treatment of essential oils showed more antimicrobial activity against both gram positive and gram negative bacteria compared to other essential oils. This higher activity of essential oil may be related to higher amount of α-pinene and β-pinene in LS2. In addition gram positive bacteria were more susceptible than gram negative bacteria.
F. Sefidkon; L. Sadeghzadeh; M. Teimouri; F. Asgari; Sh. Ahmadi
Volume 23, Issue 2 , August 2007, , Pages 174-182
Abstract
The genus Satureja represents 15 species in Iran, 9 of them are endemic. In this study, the aerial parts of Satureja khuzistanica Jamzad and Satureja bachtiarica Bunge were collected at two stage of plant growth (before flowering and full flowering) from their natural habitats. After drying the plant ...
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The genus Satureja represents 15 species in Iran, 9 of them are endemic. In this study, the aerial parts of Satureja khuzistanica Jamzad and Satureja bachtiarica Bunge were collected at two stage of plant growth (before flowering and full flowering) from their natural habitats. After drying the plant materials in shade, essential oils were obtained by hydro-distillation and analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. The results showed the oil of S. bachtiarica contained 20% carvacrol and 19% thymol before flowering and 26% carvacrol and 5% thymol at full flowering stage, as main components. The oil of S. khuzistanica, in both harvesting time contained about 90% carvacrol. Due to the antimicrobial effect of phenolic compounds, thymol and carvacrol, the antimicrobial effects of these oils were determined against five gram positive bacteria (Bacillus subtilis, Bacillus cereus, Micrococcus loteus, Staphylococcussp. and Staphylococcus areous) and three gram negative bacteria (Kellebsiella pneumonia, Kellebsiella oxytoca and Pseudomonas aeroginosa). The result showed the oil of S. khuzistanica had strong anti-bacterial effect in both harvesting stage. The anti-bacterial effect of S. bachtiarica oil was stronger before flowering stage, because of more percentage of phenolic compounds. So these oils can be used instead of synthetic antibiotics that their resistance against bacteria increased daily.
