E. Afshari; G.A Ranjbar; S.K. Kazemitabar; M. Riasat; H. Kazemi Poshtmasari
Abstract
Doubling the number of chromosomes in a medicinal plant leads to variation in allelic composition and diversity of active enzymes in secondary metabolites biosynthesis route and can facilitate the process of secondary metabolism and increase secondary metabolite. This experiment was conducted in order ...
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Doubling the number of chromosomes in a medicinal plant leads to variation in allelic composition and diversity of active enzymes in secondary metabolites biosynthesis route and can facilitate the process of secondary metabolism and increase secondary metabolite. This experiment was conducted in order to study the effect of colchicine and trifluralin on ploidy induction and cytogenetic characteristics of cells in the root meristem of fenugreek. Root tips were used for karyotypic studies. Seedlings were treated by colchicine solution at 0.25, 0.5 and 1 gl-1 concentrations and trifluralin solution 48% at 7.5, 15 and 22.5 μM concentrations for 12 and 24 h. The Video Analysis System was used for karyotype analysis. The basic chromosome number was X=8. Results showed that concentration of trifluralin and colchicine, treatments duration and interaction between them on the ploidy induction were statistically significant. The maximum ploidy induction was happened by seedling immersion in 22.5 μM trifluralin for 24 h and in 0.5 gl-1 colchicine for 12 h. Treatments affected the length of chromosomes and karyotypic formula. Result of analysis of variance based on completely randomized design (CRD) showed significant differences among the karyotype of control and treated samples for all karyotypic traits (p <0.01). Induced polyploidy affected by trifluralin was more than that of colchicines, although the concentrations of trifluralin were approximately 100 times lower than concentrations of colchicine. This indicates a greater ability of trifluralin in ploidy induction in this plant.
E. Afshari; Gh. Ranjbar; S.K. Kazemitabar; M. Riasat; H. Kazemi Poshtmasari
Abstract
In order to study callus induction, somatic embryogenesis and plant regeneration in fenugreek (Trigonella foenum-graecum L.) an experiment was conducted in Sari Agricultural Sciences and Natural Resources University during 2008. In the current experiment, different explants including stem segments, embryos, ...
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In order to study callus induction, somatic embryogenesis and plant regeneration in fenugreek (Trigonella foenum-graecum L.) an experiment was conducted in Sari Agricultural Sciences and Natural Resources University during 2008. In the current experiment, different explants including stem segments, embryos, hypocotyls, etc. were cultured on two basal culture media viz. B5 and MS. Moreover, 2, 4-D, Kin, NAA, BAP and IBA were used as plant growth regulators. Result showed that the medium containing 2mgl-1 2, 4-D in combination with 0.5mgl-1Kinetin was the best treatment for callus induction in both MS and B5 media. Combination of 0.5mgl-1 NAA and 2.5mgl-1 BAP was the best treatment for somatic embryogenesis in both basal media. Also, combination of 1.5mgl-1 BAP and 0.5mgl-1 NAA was the best hormonal treatment to shoot regeneration in both basal media. According to the results, the treatment containing 1 mgl-1 IBA was optimum for root induction from regenerated shoots on MS medium.
S. Dehghan Kouhestani; A. Baghizadeh; Gh.A. Ranjbar; N.A. Babaiyan Jelodar
Volume 24, Issue 4 , February 2009, , Pages 414-427
Abstract
Persian Cumin, a medicinal and aromatic plant, belongs to Apiaceae family. The objective of this study was to assess the genetic variation in germplasm of Persian Cumin collected from Kerman province using RAPD molecular markers. The Persian Cumin fruits were collected from 43 various regions in Kerman ...
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Persian Cumin, a medicinal and aromatic plant, belongs to Apiaceae family. The objective of this study was to assess the genetic variation in germplasm of Persian Cumin collected from Kerman province using RAPD molecular markers. The Persian Cumin fruits were collected from 43 various regions in Kerman province. DNA was extracted from fruits using modified CTAB method. From the 27 used primers in PCR, 19 primers with better bands were selected for analysis. The 446 polymorphic bands obtained by these primers were scored. The binary matrix was converted to distance matrix by applying Dice similarity coefficient in NTSYS-pc (Ver 2.02) software. Then, the distance matrix was analyzed using UPGMA and the phyllogenic dendrogram was plotted. Based on the results, investigated populations were clustered in 7 groups. The obtained clusters based on RAPD markers to some extent matched with the geographical origin of the studied populations of Persian Cumin. Furthermore, the obtained results of principal component analysis method were similar to the results of cluster analysis. The results indicated that RAPD technique is an efficient tool for assessing genetic diversity in these populations.
