F. Fakhari; F. Sefidkon; Sh. Mozaffari; M.A. Alizadeh
Abstract
The Anthemis genus, belonging to Asteraceae family, has 23 endemic annual species in Iran, with the flowers and shoots containing essential oil. In this experiment, for the first time, the seeds of Anthemis tinctoria L. were collected from west Azarbayejan, Iran, and cultivated at the field of Alborz ...
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The Anthemis genus, belonging to Asteraceae family, has 23 endemic annual species in Iran, with the flowers and shoots containing essential oil. In this experiment, for the first time, the seeds of Anthemis tinctoria L. were collected from west Azarbayejan, Iran, and cultivated at the field of Alborz Research Station. In order to determine the essential oil composition of cultivated Anthemis tinctoria, random sampling was conducted at full flowering stage from flowering shoot. Flowers, leaves and stems were divided and dried in open air and their essential oil was produced separately by hydro-distillation. To analyze the essential oil composition, GC and GC-MS were used. Results indicated that essential oil percentage in flower, leaf, stem and total shoot was 0.0892, 0.0487, 0.0466 and 0.0792%, respectively. Analysis of leaf essential oil indicated that 32 compounds were detected; five main compounds were camphor (15.8%), α-eudesmol (10.3%), guaiol (9.6%), chrysanthenone (8.4%) and 1,8-cineol (8.0%); contributing to more than 53% of the essential oil composition. Twenty-one compounds were detected in stem essential oil; four main compounds were spathulenol (25.6%), α-eudesmol (14.5%), caryophyllene oxide (11.8%) and β-eudesmol (5.9%). Moreover, 22 compounds were detected in flower essential oil; five main compounds were α-eudesmol (27.5%), β-eudesmol (14.3%), spathulenol (13.3%), 10-epi-g-eudesmol (7.2%), 1,8-cineol (4.2%). The results also indicated that 29 compounds were detected in shoot essential oil; four main compounds were g-eudesmol (39.92%), caronene (9.1%), camphor (6.5%), spathulenol (5.1%) and 1,8-cineol (4.3%). According to the obtained results, the percentage of α-eudesmol as main component of this essential oil varied from 10 to 40% in different plant parts, the lowest in leaf oil and the highest in aerial part oil. The highest amount of β-eudesmol was found in flower and stem oil. The presence of high amount of chrysanthenone and camphor in leaf oil was also remarkable. It can be concluded that by using flowering shoot or total aerial parts of A. tinctoria in full flowering stage, more essential oil yield would be produced. In addition, for special purposes and obtaining some special compounds, special plant parts could be distilled.
A.A Hossienpour Ggazviniy; M.A. Alizadeh; A.A. Jafari; A.R. Valadabadi
Abstract
In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised ...
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In order to study the dormancy breaking ways and germination enhancment, seeds of 8 ecotypes of 4 species Satureja sahendica Bornm., S. bachtiarica Bunge, S. khuzistanica Jamzad and S. hortensis L. were collected on the base of geographycal distributions. A factorial experiment based on completely randomised design with three replications were used. The different treatments including: cold stratification, after-ripening, physical scarification (sand paper), chemical scarification (Ethylic alcohol 70%) and control were used for dormancy breaking. The treated seeds were sown in 3 petri dishes as replications. Then, all samples were placed in a germinator with condition of 20°C and light-to-dark cycle of 16 hours light (1000 lux) to 8 hours dark. The germination characteristics including: germination percentage, speed of germination, length of root and shoot, vigour index, fresh weight and dry weight were evaluated during 18 days of the experiment. There were significant differences amoung species for all seed germination characteristics. The ecotype of Khuzistan from S. khuzistanica had more seed germination and vigour compared to the other three species. The seed characteristics specially speed of germination and vigour index of ecotypes from cold zone were affected by cold treatment more than the other treatments.
M.A. Alizadeh
Abstract
Model of seed storage behavior has high importance for seed conservation. With determination model of seed storage behavior, collection and conservation of plant seed would be recognized. If the plant seeds have orthodox behavior, their conservation will be carried out in out of provenance and it is ...
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Model of seed storage behavior has high importance for seed conservation. With determination model of seed storage behavior, collection and conservation of plant seed would be recognized. If the plant seeds have orthodox behavior, their conservation will be carried out in out of provenance and it is termed as Ex- situ conservation. If the plant seeds have recalcitrant behavior, their conservation would be done within provenance and it is termed as In situ conservation. In this research, model of seed storage behavior of some medicinal plant seeds were studied. The seed samplesof some medicinal plants from different state of country were processed and analyzed. The processing of the seed samples included physical and genetic purity, seed healthy test, thousand weight, moisture content determination, germination test, packaging and conservation of the seed samples in cold room of gene bank. Evaluation and determination of model of seed storage behavior was the major point of this research work. By determination of moisture content, the model of seed storage behavior of medicinal plant seeds was identified and most of them have orthodox seed storage behavior. Therefore their conservation should be done as Ex- situ conservation.
M.A. Alizadeh; H.R. Isvand
Volume 20, Issue 3 , October 2004, , Pages 301-307
Abstract
The majority of the crop seeds have physiological dormancy after harvesting. This phenomenon is depending to different physiological dormancy. This phenomenon can overcome by pre-treatment including prechilling, pre-heating, dry storage condition, using chemical for promoting germination and overcoming ...
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The majority of the crop seeds have physiological dormancy after harvesting. This phenomenon is depending to different physiological dormancy. This phenomenon can overcome by pre-treatment including prechilling, pre-heating, dry storage condition, using chemical for promoting germination and overcoming of inhibitor substances. In this study the seeds of two medicinal species (Eruca sativa Lam. and Anthemis altissima L.) under two cold room (4°C) and dry storage (room temperature) conditions were maintained over six months and tested by standard germination test. The physiological quality of the seed rocket plant had not any significance differences in two conditions. The reason might be due to non-physiological dormancy of the seed species. Whereas, percent of germination in the species of chamomile was significant in the two conditions. As it is clear the percent of germination of the chamomile from dry storage condition was higher from cold room condition and this due to physiological dormancy of plant seed which overcome by dry storage condition. The speed and vigor index of plant seed had no significant differences in two conditions.
