F. Nosrati; B. Fakheri; M. Solouki; N. Mahdi Nezhad; M. Valizadeh
Abstract
Sarcocolla(Astragalus fasciculifolius Boiss.), belonging to Fabaceae family, is a medicinal plant distributed in the south of Sistan and Baluchestan province. Due to presence of compounds like saponins, flavonoids, and polysaccharides, it has an effective role in treating heart disease and cancer and ...
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Sarcocolla(Astragalus fasciculifolius Boiss.), belonging to Fabaceae family, is a medicinal plant distributed in the south of Sistan and Baluchestan province. Due to presence of compounds like saponins, flavonoids, and polysaccharides, it has an effective role in treating heart disease and cancer and eliminating chemotherapy and improving immunity. In this study, plant samples including eight A. fasciculifolius were collected from different habits in the province, and some phytochemical properties of root, leaves (total phenol, total flavonoid, and polysaccharide) and of flowers (total phenol, total flavonoid, and anthocyanin) were analyzed in the plant flower. The results of variance analysis showed a significant difference among different habitats (α>0.01). Phytochemical studies revealed significant amounts of phenolic compounds in the leaf with the highest rate recorded in Poshtkooh (14.62-32.55 mg GAE/g). In the root, the highest value was in Anjirok (20.39-53.84 mg GAE/g) and in the flower, the highest value was in BAirk (5.11-15.82 mg GAE/g), representing flavonoid compounds in the leaf in which the highest value was in Nahook (8.76-12.72 mg QE/g). Also, in root, the highest value was in Panjangosht (10 fingers) (0.25-1.03 mg QE/g). In the flower, the highest value was in Nahook (6.35-9.28 mg QE/g). In the polysaccharide compounds of the leaf, the highest value was in Anjirok (60.89-93.29 mg/g). In root, the highest values were in Pangangosht (94.33-209.38 mg/g) and anthocyanin of flower had the highest values in Pangangosht (36.34-77.58 mg/g). The results of this study represent the ability of different habitats of the province in producing valuable herbal compounds. Besides, because of its high levels of phenolic and polysaccharide compounds, this plant has a remarkable capability to be used in food and pharmaceutical industries.
F. Nosrati; B. Fakheri; M. Solouki; N. Mahdi Nezhad; M. Valizadeh
Abstract
Development and implementation of effective and useful programs for the rehabilitation, conservation and sustainable utilization of medicinal species is possible only by identifying plant and ecological characteristics. Astragalus fasciculifolius Bioss. belonging to the Fabaceae family, is a valuable ...
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Development and implementation of effective and useful programs for the rehabilitation, conservation and sustainable utilization of medicinal species is possible only by identifying plant and ecological characteristics. Astragalus fasciculifolius Bioss. belonging to the Fabaceae family, is a valuable medicinal species. This species plays an effective role in treatment of heart disease, inhibition of cancer cell growth, and eliminating the chemotherapy effects due to the presence of compounds such as saponins, flavonoids, and polysaccharides. In this study, the distribution map of this species was prepared using available resources, and then climatic characteristics, physical and chemical analysis of soil, different phenological stages, and associated plants in the region were recorded. The results of this study showed that this plant is distributed in the cities of Khash, Saravan, Soran and Mehrestan. According to the Demartonne method, the climate of this region is arid. The average annual precipitation, relative humidity, and an average temperature of these habitats are 52 mm, 20%, and 25°C, respectively. This species is distributed between approximately 1300 and 1500 m above sea level, on the slopes and mainly in the waterways, in the bed of seasonal rivers, and in all directions, especially the northern direction and the slope between 0 to 75%. The soil texture of habits is mainly sandy loam with the pH range of 6.73 to 8.28, EC range of 0.56 to 1.06 ds/m, and 30.64-50.62% lime. A. fasciculifolius is a drought tolerant species which is capable of growing under dry climatic conditions with an average annual rainfall of less than 30mm.
H. Mousavi; N. Mahdi Nezhad; B. Fakheri; M. Majdi; F. Heidari
Abstract
To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium ...
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To investigate the effects of nano-chelate spraying of Iron, chemical -synthesis and green-synthesis silver nanoparticles on the expression of two genes, germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes which are involved in the parthenolide biosynthesis pathway of Tanacetum parthenium L., a factorial experiment based on randomized complete block design was conducted with three replications. The experiment took place in the greenhouse of Zabol University in Iran. At first, the total RNA from samples of leaves, and then the synthesis of cDNA were extracted. Afterward, Real Time PCR analysis was used to determine gene expression patterns. Parthenolide concentration was measured by high-performance liquid chromatography (HPLC). The analysis of the data was conducted by SAS software, and there was a significant increase in the expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes of treated plants with green-synthesis silver nanoparticles in compare with the control plants, and some plants which were treated by chemical- synthesis silver nanoparticles and nano-chelate of Iron. Furthermore, the maximum expressions of both germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) genes were identified in groups of plants which were under the influence of drought stress, or were treated by green-synthesis silver nanoparticles. A positive relation was seen between the expressions of germacrene A synthase genes (TpGAS) and parthenolide synthase (TpPTS) genes with the amount of parthenolide itself. It can be concluded that green-synthesis silver nanoparticles and water deficit stress can cause an increase on the expressions of parthenolide and germacrene A synthase genes, and as result of that, cause an increase on parthenolide production of Tanacetum parthenium.
