Biotechnology
Z. Abravesh; H. Zare; M. Khoshnevis
Abstract
Sorbus persica Hedl. is an Iranian endemic slow-growing tree (fam. rosaceae) and is endangered. It is important in terms of the gene storage, environmental protection, and medicinal uses. To in vitro propagate this species by the lateral bud culture, a factorial experiment was conducted in a completely ...
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Sorbus persica Hedl. is an Iranian endemic slow-growing tree (fam. rosaceae) and is endangered. It is important in terms of the gene storage, environmental protection, and medicinal uses. To in vitro propagate this species by the lateral bud culture, a factorial experiment was conducted in a completely randomized design in three levels of culture medium and three levels of cytokinin with three replications. For shooting, the DKW, WPM, and MS culture media containing the cytokinins BAP, Kin, and 2ip, and for rooting, the complete and modified DKW, WPM, and MS culture media containing the auxins NAA and IBA separately and in a consecutive application were investigated. The results showed that the best sterilization treatment was a consecutive application of sodium hypochlorite 20% (v/v, 15 min) and mercuric chloride 0.1% (3 min) in summer. The best proliferation and longitudinal growth of shoots was observed in the MS medium containing IBA (0.01 mg l-1), Kin (0.25 mg l-1), and BAP (3 mg l-1) growth regulators. Also, the best rooting was obtained in the DKW medium with a quarter of the concentration of macroelements containing the NAA+IBA hormones (0.3+0.3 mg l-1).
Z. Abravesh; M.B. Rezaee; F. Ashrafi
Volume 20, Issue 4 , January 2018, , Pages 457-468
Abstract
Many of crude products from medicinal plants, becaused of essential oils used in medical. But in most of crude products, essential oils seprated and used as a medicine. The purpose of this study was evalution of essential oils extracted by hydrodistellation method from Salvia officinalis L. ...
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Many of crude products from medicinal plants, becaused of essential oils used in medical. But in most of crude products, essential oils seprated and used as a medicine. The purpose of this study was evalution of essential oils extracted by hydrodistellation method from Salvia officinalis L. and effected on four bacteries (g+) and (g -) consist: E.coli(g-), Shigella sonnei (g), Bacillus anthracis (g+) and Bacillus cereus (g+), by using well diffusion method and chemical constituents of essential oils analyzed and determined by GC and GC/MS. Main compounds consist: pinene (5.5%), borneol (9.4%), humulene (8.4%) and globulol (9.3%) .Essential oil effective identified against above mentioned bacterial susspansion by micro organism 10 8 Colony Forming Unit / ml (cfu/ml) until is studied bacteriocide amount.Bacteriocide activity of Salvia officinalis L. shoot flowers essential oil observed later of 24-48 h zones of growth inhibition respectiviely Bacilluscereus, 40 mm,Bacillus anthracis, 25 mm,Shigella sonnei24 mm, E. coli20 mm.
M.H. Assareh; Z. Abravesh; S.R. Tabaei Aghdaei
Abstract
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature ...
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Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature shrubs. The branches were laid in sand bed, soaked for 24 h by different concentrations of IBA and NAA hormones. The results showed that the rate of rooting was high in sand bedding material in autumn and winter. The highest rooting percentage was %100 with 50 mgl-1 concentration of IBA in autumn and winter seasons and the lowest of rooting percentage was %53.33 with 50 mgl-1 concentration of NAA in autumn season. The rooting branches were cut from the parent plants after 3 months and planted in pots containing soil: sand: peatmoss complex (1:1:1) in greenhouse conditions, survival percentage was 90%.
E. Fathi; F. Sefidkon; Gh. Bakhshi Khaniki; Z. Abravesh; M.H. Assareh
Volume 25, Issue 1 , May 2009, , Pages 64-74
Abstract
In this research, the fresh leaves of Eucalyptus largiflorens were collected in the middle of spring from Kashan in Isfahan province. After drying the plant materials in oven 30°C, 40°C, 50°C, sun and shade, their essential oils were obtained by hydro-distillation. In addition, the essential ...
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In this research, the fresh leaves of Eucalyptus largiflorens were collected in the middle of spring from Kashan in Isfahan province. After drying the plant materials in oven 30°C, 40°C, 50°C, sun and shade, their essential oils were obtained by hydro-distillation. In addition, the essential oil of shade-dried sample was obtained by two other distillation methods (water and steam distillation and direct steam distillation). The oils were analyzed by GC and GC/MS. Oil yields (w/w) of the oven-dried 30°C, 40°C, 50°C, sun-dried and shade-dried sample were 1.37%, 1.58%, 1.59%, 1.31% and 1.32% respectively. 1,8-cineole (25.0%, 26.2%, 40.6%, 29.7% and 24.6%), P-cymene (17.2%, 17.4%, 20.3%, 20.5% and 17.1%) and α-pinene (16.2%, 12.6%, 14.4%, 7.2% and 9.9%) were identified as the main components in oven-dried 30°C, 40°C, 50°C, sun-dried and shade-dried sample, respectively. On the other hand, the oil yields were 0.92% and 0.77% in water and steam distillation and direct steam distillation. In different drying methods the result showed the oil yield and 1,8-cineole percentage were higher in oven-dried 50°C sample and in different distillation the oil yield in hydro-distillation was higher and the highest percentage of 1,8-cineole was obtained by water and steam distillation.
Z. Abravesh; F. Sefidkon; M.H. Assareh
Volume 23, Issue 3 , November 2007, , Pages 323-330
Abstract
The fresh leaves of five cultivated Eucalyptus species, i.e. Eucalyptus stricklandii Maiden, E. brockwayii, E. sargentii Maiden, E. largiflorens F. Muelland E. kruseana F. Muell were collected in the spring from Shushtar in Khuzestan province (South region of Iran). After drying the plant materials in ...
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The fresh leaves of five cultivated Eucalyptus species, i.e. Eucalyptus stricklandii Maiden, E. brockwayii, E. sargentii Maiden, E. largiflorens F. Muelland E. kruseana F. Muell were collected in the spring from Shushtar in Khuzestan province (South region of Iran). After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by GC and GC/MS. Seventeen components were identified in the oil of E. stricklandii with 1,8-cineole (71.2%) and α-pinene (9.2%) as the main constituents. Twenty-five compounds were identified in the oil of E. brockwayii with 1,8-cineole (17.8 %), isopentyl isovalerate (17.2%), α-pinene (14.0%), trans-pinocarveole (12%), β-pinene(7.5%) and ρ-cymene ( 5.3%) as major components. Sixteen compounds were characterized in the oil of E. sargentii with 1,8-cineole (56.7%), β-eudesmol (6.0%) and α-pinene (4.9%) as the main constituents. Fifteen components were identified in the oil of E. largiflorens with 1,8-cineole (41.3%), spathulenol (11.6%) and virdiflorol (15.9%) as major components. Fifteen components were identified in the oil of E. kruseana with 1,8-cineole (63.3%) and α-pinene (15.9%) as the main constituents. The results showed, although 1,8-cineole was the main component of the essential oils of all the studied Eucalyptus species, its relative content was higher in the oil of E. stricklandii.
F. Sefidkon; M.H. Assareh; M. Mirza; Z. Abravesh; M.H. Salehe Shushtari
Volume 23, Issue 1 , May 2007, , Pages 39-50
Abstract
In this investigation, the leaves of five Eucalyptus species as Eucalyptus microtheca F. Muell, E. gongylocarpa Blakely, E. gillii Maiden, E. woodwardi Maiden and E. salubris F. Muell were collected from two regions of Khuzistan province (Shushtar and Dezful, South Iran) in spring. After drying the plant ...
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In this investigation, the leaves of five Eucalyptus species as Eucalyptus microtheca F. Muell, E. gongylocarpa Blakely, E. gillii Maiden, E. woodwardi Maiden and E. salubris F. Muell were collected from two regions of Khuzistan province (Shushtar and Dezful, South Iran) in spring. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. The oils of E. microtheca were obtained at yields of 0.43% and 0.37% (w/w) from Shushtar and dezful, respectively. These yields were 2.37% and 1.78% for E. gongylocarpa, 3.5% and 3.9% for E. gillii, 1.18% and 1.13% for E. woodwardi, 2.05% and 1.02% for E. salubris. The main compounds of E. microtheca oils were 1,8-cineole (26.7% and 16.9%), P-cymene (14.8% and 20.4%) and a-pinene (12.5% and 12.0%) in the Shushtar and Dezful samples, respectively. The major constituents of E. gongylocarpa oils were 1,8-cineole (66.8% and 78.6%) and a-pinene (5.2% and 7.2%). The main components of E. gillii oils were 1,8-cineole (58.5% and 81.3%) and a-pinene (13.9% and 11.0%). The main compounds of E. woodwardi oils were 1,8-cineole (58.7% and 45.1%) and a-pinene (23.1% and 21.4%). The major constituents of E. salubrisoils were 1,8-cineole (62.0% and 71.0%) and a-pinene (8.0% and 10.0%). Differences in the percentage of the main components of these oils in two regions could be due to some differences in habitats like soil structure or humidity.
M.H. Assareh; Z. Abravesh; M.B. Rezaee
Abstract
The leaves of Eucalyptus caesia were collected from north khuzistan in March 2003. The essential oil of Eucalyptus caesia (Myrthaceae) was prepared by hydro-distillation and analyzed by GC and GC/MS. The essential oil was produced at yield of 0.97% (based on dry weights). Twenty-one components were identified, ...
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The leaves of Eucalyptus caesia were collected from north khuzistan in March 2003. The essential oil of Eucalyptus caesia (Myrthaceae) was prepared by hydro-distillation and analyzed by GC and GC/MS. The essential oil was produced at yield of 0.97% (based on dry weights). Twenty-one components were identified, among them α-pinene (9.3%), 1,8-cineole (69.4%), trans-pinocarveole (2.4%), caryophyllene (6.1%) and globulol (2.8%) were the major constituents.
K. Jaimand; M.B. Rezaee; Z. Abravesh; M. Golypour; M. Sharifee
Abstract
Oleuropein, a compound that cause the bitter taste of olive, has many pharmacological properties. It is a natural antioxidant. In this study, the leaves of nine cultivars of Olea europaea L. named: Olive Begonia, Olive Gorgan, Olive Dezful, Olive Khshavy, Olive Khoramabady, Olive Dagal, Olive Barbar, ...
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Oleuropein, a compound that cause the bitter taste of olive, has many pharmacological properties. It is a natural antioxidant. In this study, the leaves of nine cultivars of Olea europaea L. named: Olive Begonia, Olive Gorgan, Olive Dezful, Olive Khshavy, Olive Khoramabady, Olive Dagal, Olive Barbar, Olive Zahedy and Olive Red were collected from Fadak station in Dezful city (Khozestan province, western south of Iran) at September 2004. After extraction of leaves by methanol, the oleuropein contents of extracts were determined by HPLC. Maximum amount of oleuropein was found in Olive Khoramabady (0.08 mg/ml, 0.24% w/w) and minimum in Olive Dagal (0.04 mg/ml, 0.13% w/w).
Z. Abravesh; A. Majd; M.B. Rezaee; S. Mehrabian
Abstract
Man used to take a lot of medicinal plants, but a little to take care of their duration. To find methods for increasing their products always paid attention to plant. Essential oil of the flowering shoot of Ammi visnaga was extracted by hydro-distillation method and the essential oil inhibitory effects ...
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Man used to take a lot of medicinal plants, but a little to take care of their duration. To find methods for increasing their products always paid attention to plant. Essential oil of the flowering shoot of Ammi visnaga was extracted by hydro-distillation method and the essential oil inhibitory effects have been studied by using well diffusion method on four bacteria consists of: Streptococcus viridans, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus acidophilus (control: cetylpyridinium chloride 0.05%). Effective essential oil against above mentioned bacteria suspension were studied by micro organism 108 colony forming unit/ml (cfu/ml). Bactericide activity of essential oil of Ammi visnaga flowering shoot observed later of 24-48 hours. The zones of growth inhibition were as follow: Streptococcus viridans 35mm, Lactobacillus casei 50mm, Lactobacillus plantarum 35mm, Lactobacillus acidophilus 35mm and control 12mm.
